BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,075,358	A→G	58 (TAA→TAG)	ymdF →	general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,075,358	0	A	G	99.4%	1770.9 / NA	511	58 (TAA→TAG)	ymdF	general stress protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (230/278); minor base T (2/1); total (232/279)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 511 total aligned reads displayed.

GAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTT‑G‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGAT  >  NZ_CP025268/1075221‑1075492

gaagaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGcc                                                                                                                                         <  2:10775779/140‑1 (MQ=255)

   gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  2:10509521/140‑1 (MQ=255)

   gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  1:6612561/140‑1 (MQ=255)

   gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  1:2024684/140‑1 (MQ=255)

   gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  1:2076826/140‑1 (MQ=255)

      cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTACTTTCAAGAATGACCCGCCGCGCGCATCTGAAGCAGGTACAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                   >  2:6628243/1‑140 (MQ=255)

      cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                   >  2:11055732/1‑140 (MQ=255)

      cgagAAAGAGCATCAGAAGCAGGTAAAAAAGGTTGACAGAACAGCGGGGGTAATTTAAAGAATGACCCGCATAGAGAATCTGAAGCAGGTAAAAAAGGTGTTAAGAGAAGTCACGGCAAAAGCGACAACTATCCAGGCTa                                                                                                                                   >  2:1604056/4‑140 (MQ=255)

        cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                                                                                                                 >  2:8836583/1‑140 (MQ=255)

                        gCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAAtgcatt                                                                                                                 <  1:9794848/140‑6 (MQ=255)

                         cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttt                                                                                                                <  2:8603954/140‑1 (MQ=255)

                         cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttt                                                                                                                <  1:1609000/140‑1 (MQ=255)

                           ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTCAAACAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttttt                                                                                                              >  2:7294625/1‑140 (MQ=255)

                           ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttttt                                                                                                              <  2:7719088/140‑1 (MQ=255)

                            gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑g                                                                                                            >  1:1751116/1‑140 (MQ=255)

                            gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑g                                                                                                            >  1:5034678/1‑140 (MQ=255)

                             tAAACACGGTGGACCGCTCAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTATAAAAGGTGGTAAGAGCAGTCACGGCAACCGCGACAACTCGCCGGGCTAATCAATGACGAATGCATTTTT‑gt                                                                                                           >  2:1072018/1‑139 (MQ=255)

                                aaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACACCTAGCCGGGCTAATCAATGACGAATGCATTTTT‑gtctg                                                                                                        >  2:7937188/1‑136 (MQ=255)

                                     gTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCt                                                                                                   <  2:2820773/140‑1 (MQ=255)

                                     gTGGACAGCACAGCGGGGGTAATGTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCt                                                                                                   <  2:7091929/140‑1 (MQ=255)

                                      tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTc                                                                                                  >  1:9253232/1‑140 (MQ=255)

                                      tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTc                                                                                                  >  2:137933/1‑140 (MQ=255)

                                       ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAATAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGG‑CTGTAGCTCg                                                                                                 >  2:9337223/1‑140 (MQ=255)

                                       ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCg                                                                                                 >  1:5356870/1‑140 (MQ=255)

                                             cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCaaat                                                                                           >  1:9531895/1‑139 (MQ=255)

                                              acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCaaaaa                                                                                          >  2:4164354/1‑140 (MQ=255)

                                                  cGGGGGTAATTTCAAGAATGACCCGCCGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCa                                                                                      >  2:5194631/1‑140 (MQ=255)

                                                     gggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCa                                                                                   >  2:2059428/1‑140 (MQ=255)

                                                     gggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCAc                                                                                  >  1:3963217/1‑140 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                 >  1:6348821/1‑140 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                 >  1:9749034/1‑140 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                 >  1:7770420/1‑140 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                 >  1:10014995/1‑140 (MQ=255)

                                                          aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCAccgcc                                                                              >  2:2583701/1‑140 (MQ=255)

                                                          aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCAccgcc                                                                              >  2:3037746/1‑140 (MQ=255)

                                                             tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                           <  1:1191872/140‑1 (MQ=255)

                                                              cAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGtt                                                                          >  1:516313/1‑140 (MQ=255)

                                                               aaGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTa                                                                         >  1:3187728/1‑140 (MQ=255)

                                                                aGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAATAAGCCATCACCGCCGGTTAc                                                                        >  1:11159018/1‑140 (MQ=255)

                                                                aGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAc                                                                        >  1:2173637/1‑140 (MQ=255)

                                                                 gactGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                       <  1:10956386/137‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAGAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     <  1:867969/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     <  2:11261263/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     <  1:10132799/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     <  1:4429336/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     <  1:10291911/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     <  1:2448222/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     <  1:10747866/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     <  1:5749750/140‑1 (MQ=255)

                                                                      aCCCGCCGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                  >  1:424088/1‑140 (MQ=255)

                                                                      aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                  >  2:3690956/1‑140 (MQ=255)

                                                                      aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                  >  1:6833719/1‑140 (MQ=255)

                                                                       cccGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                     >  1:9645387/1‑136 (MQ=255)

                                                                        ccGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                                <  1:10408270/140‑1 (MQ=255)

                                                                          gCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTg                                                              <  1:1446542/140‑1 (MQ=255)

                                                                           cAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGa                                                             >  1:9001403/1‑140 (MQ=255)

                                                                                cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                        >  1:3642834/1‑140 (MQ=255)

                                                                                cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                        >  2:6993100/1‑140 (MQ=255)

                                                                                    tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAATTTT‑GTCTGTATCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgatga                                                    <  2:5935390/140‑1 (MQ=255)

                                                                                     cTGCAGCAGGCACACCAGGTGGTACGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAGCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                   <  2:9709775/140‑1 (MQ=255)

                                                                                     cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                   <  2:5577079/140‑1 (MQ=255)

                                                                                     cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                   <  2:3990933/140‑1 (MQ=255)

                                                                                     cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                   <  2:4808825/140‑1 (MQ=255)

                                                                                         cgCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAACAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAACGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                               <  2:6349418/139‑1 (MQ=255)

                                                                                         aGCAGTGAAACAAGGTTGTAAGAGCAGGCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACTAATGCATTTTT‑GTCTGTAGCTCTTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                               <  2:9043488/140‑1 (MQ=255)

                                                                                          gCAGGTAAAAAAGGGGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATg                                              >  2:2943717/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                           >  1:6266598/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                           >  2:2493622/1‑140 (MQ=255)

                                                                                                       gtggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGa                                 <  2:919500/140‑1 (MQ=255)

                                                                                                        tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                                <  2:1217225/108‑1 (MQ=255)

                                                                                                        tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                                >  1:4732784/1‑140 (MQ=255)

                                                                                                        tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                                >  1:8854253/1‑140 (MQ=255)

                                                                                                        tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                                >  1:8275120/1‑140 (MQ=255)

                                                                                                           tAAGCGCAGTCACGGCAAAAGCGACAACTCGCCGGGCGACTCAATGACGCATGCAATTTT‑GTCTGGAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                             <  2:3819080/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTCGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCAGATgcg                             <  2:2136242/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                             <  1:265391/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                             <  1:8452036/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                             <  1:2883786/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                             <  1:3014473/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                             <  1:3730977/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                             <  1:4823607/140‑1 (MQ=255)

                                                                                                                gCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                        <  1:3354245/140‑1 (MQ=255)

                                                                                                                  aGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCt                      >  2:1907893/1‑140 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                    >  2:3306890/1‑140 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                    <  1:7332749/140‑1 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                    <  2:7469362/140‑1 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                    <  1:5558932/140‑1 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                    <  1:11378031/140‑1 (MQ=255)

                                                                                                                    tCAAGGCAAAAGCGACAACTAGCCGGGCTAAACAAAGACGAATGCAATTTT‑GTCAGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGAGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                    <  2:10043364/140‑1 (MQ=255)

                                                                                                                         gCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg               >  2:4557252/1‑140 (MQ=255)

                                                                                                                           aaaaGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGcc             <  2:4342106/140‑1 (MQ=255)

                                                                                                                           aaaaGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGcc             <  2:6158948/140‑1 (MQ=255)

                                                                                                                           aaaaGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGcc             <  1:3862113/140‑1 (MQ=255)

                                                                                                                              aGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc          >  1:7411582/1‑140 (MQ=255)

                                                                                                                              aGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc          >  2:10174925/1‑140 (MQ=255)

                                                                                                                                cGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCa        >  1:7127986/1‑140 (MQ=255)

                                                                                                                                  aCAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAATGCCATCACCGCCGGTAACGCGGGGGTCGATTCTTTGGACAGATGAAAGCTTGGCTGTGGCGCGTTGCTTAGCAGGCCTACCaga      >  1:6939746/1‑140 (MQ=255)

                                                                                                                                  aCAACTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCaga      >  1:1881067/1‑140 (MQ=255)

                                                                                                                                   caccTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa     <  2:4983565/137‑1 (MQ=255)

                                                                                                                                     aCTAGCCGGGCTAATCAATGACGAATGCATTTTT‑GTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga   <  2:8248693/140‑1 (MQ=255)

GAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAAT‑CATTTTT‑G‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGAT  >  NZ_CP025268/1075221‑1075492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`