Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,219,333 G→A intergenic (‑230/+27) iraM ← / ← CXP41_RS24360 anti‑adapter protein IraM/protein YmgK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,219,3330GA100.0% 175.0 / NA 46intergenic (‑230/+27)iraM/CXP41_RS24360anti‑adapter protein IraM/protein YmgK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/27);  total (19/27)

TAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATGCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTC  >  NZ_CP025268/1219198‑1219466
                                                                                                                                       |                                                                                                                                     
taaatTAATTAAAATGTTGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACAtt                                                                                                                                   >  1:2948973/1‑140 (MQ=255)
    ataattaaAATGATGGCGTATATAAAATAAAATTTCAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCaaa                                                                                                                               <  1:9074283/139‑1 (MQ=255)
       attaaAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGa                                                                                                                            <  1:6344842/140‑1 (MQ=255)
                                                      aaTGGATGATAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGATTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCa                                                                             <  1:8733978/140‑1 (MQ=255)
                                                            tGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTCTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGc                                                                       >  1:7953151/1‑140 (MQ=255)
                                                            tGTTAAACAAAATGAGATTTTGAGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACAAAGCCTTTATGCTACTTTGTGCCAATTAGc                                                                       >  1:7953155/1‑140 (MQ=255)
                                                             gTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCt                                                                      >  1:7373050/1‑140 (MQ=255)
                                                              ttAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTa                                                                     >  1:3153022/1‑140 (MQ=255)
                                                              ttAAACAAAATGAGATTTTGAGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTa                                                                     <  1:11340777/140‑1 (MQ=255)
                                                                  aCAATATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACa                                                                 <  2:4120484/140‑1 (MQ=255)
                                                                  aCAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACa                                                                 <  2:4120292/140‑1 (MQ=255)
                                                                  aCAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACa                                                                 <  2:4120489/140‑1 (MQ=255)
                                                                  aCAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACa                                                                 <  1:9068863/140‑1 (MQ=255)
                                                                  aCAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACa                                                                 <  1:10141643/140‑1 (MQ=255)
                                                                           gaTTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTg                                                        <  1:8774172/140‑1 (MQ=255)
                                                                           gaTTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTg                                                        <  1:1192012/140‑1 (MQ=255)
                                                                                      aGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTtatat                                             >  2:10490941/1‑140 (MQ=255)
                                                                                        cAATAAATTATTGACTTCGTTTTAGATTTGTATAGCGATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAACACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATaa                                           <  2:8850387/140‑1 (MQ=255)
                                                                                                  ttGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATaataat                                 <  1:7468738/140‑1 (MQ=255)
                                                                                                  ttGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATaataat                                 >  1:2203589/1‑140 (MQ=255)
                                                                                                   tGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTTTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAAAGATAATAATg                                >  2:8186168/1‑140 (MQ=255)
                                                                                                       ttCGTTTTAGATTTTTTTAGCTATAATGTTATACATTCATATGAATGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAata                            <  2:7953154/140‑1 (MQ=255)
                                                                                                       ttCGTTTTAGATTTGTCTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAata                            <  2:7953150/140‑1 (MQ=255)
                                                                                                        tCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAatat                           >  2:7569945/1‑140 (MQ=255)
                                                                                                         cGTTTTAGATTTGTTTAGCTATAATGTTATACATTCa                                                                                                                                 >  1:9663628/1‑37 (MQ=255)
                                                                                                         cGTTTTAGATTTGTTTAGCTATAATGTTATACATTCa                                                                                                                                 <  2:9663627/37‑1 (MQ=255)
                                                                                                                 aTTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACt                                                                                     <  2:4589540/73‑1 (MQ=255)
                                                                                                                 aTTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACt                                                                                     <  2:4589528/73‑1 (MQ=255)
                                                                                                                 aTTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACt                                                                                     <  2:9704416/73‑1 (MQ=255)
                                                                                                                 aTTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACt                                                                                     >  1:4589528/1‑73 (MQ=255)
                                                                                                                 aTTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACt                                                                                     >  1:4589540/1‑73 (MQ=255)
                                                                                                                 aTTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACt                                                                                     >  1:9704417/1‑73 (MQ=255)
                                                                                                                  tttGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCATGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGcatgaca                 <  2:2948973/140‑1 (MQ=255)
                                                                                                                     gTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGt                                                          >  2:9635140/1‑96 (MQ=255)
                                                                                                                     gTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGt                                                          <  1:9635141/96‑1 (MQ=255)
                                                                                                                     gTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGcatgacatga              >  2:4847543/1‑140 (MQ=255)
                                                                                                                          gCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATa         <  1:4847543/140‑1 (MQ=255)
                                                                                                                              taATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGt     >  2:4194398/1‑140 (MQ=255)
                                                                                                                                aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTa                                                                     >  2:2061157/1‑74 (MQ=255)
                                                                                                                                aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTa                                                                     <  1:2061157/74‑1 (MQ=255)
                                                                                                                                aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGtt                                                         >  2:11017427/1‑86 (MQ=255)
                                                                                                                                aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGtt                                                         <  1:11017428/86‑1 (MQ=255)
                                                                                                                                aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGt                                                          <  2:11111983/85‑1 (MQ=255)
                                                                                                                                aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGt                                                          >  1:11111984/1‑85 (MQ=255)
                                                                                                                                aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGttt   <  1:4194398/140‑1 (MQ=255)
                                                                                                                                 tGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTc  <  2:10320230/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATGCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTC  >  NZ_CP025268/1219198‑1219466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑