BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,236,662	T→C	A277A (GCA→GCG)	hlyE ←	hemolysin HlyE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,236,662	0	T	C	99.8%	6632.8 / NA	1897	A277A (GCA→GCG)	hlyE	hemolysin HlyE
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (1170/722); minor base A (2/1); total (1174/723)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1904 total aligned reads displayed.

TGTTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCTGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCG  >  NZ_CP025268/1236525‑1236798

 gtTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGgccgct                                                                                                                                       <  1:8452926/140‑1 (MQ=255)

           gtgATAAACCTTATACCTTGAGTACATGGAGAGAATACTCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCa                                                                                                                             <  1:4520761/140‑1 (MQ=255)

           gtgATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCa                                                                                                                             <  1:1111180/140‑1 (MQ=255)

              aTAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCaaag                                                                                                                          >  2:5534839/1‑140 (MQ=255)

                aaaCCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCaaagaa                                                                                                                        <  2:4256987/140‑1 (MQ=255)

                aaaCCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCaaagaa                                                                                                                        <  2:11417502/140‑1 (MQ=255)

                     ttATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAt                                                                                                                   <  2:8971461/140‑1 (MQ=255)

                      tataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAtt                                                                                                                  >  2:8338032/1‑140 (MQ=255)

                      tataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAtt                                                                                                                  >  2:4766305/1‑140 (MQ=255)

                       ataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTa                                                                                                                 >  1:2630744/1‑140 (MQ=255)

                        taCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaa                                                                                                                <  1:4000049/140‑1 (MQ=255)

                        taCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaa                                                                                                                >  1:2325939/1‑140 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  2:5321292/140‑1 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  2:7634545/140‑1 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:2828327/140‑1 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:10952497/140‑1 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  2:1254699/140‑1 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:8077116/140‑1 (MQ=255)

                           cTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatc                                                                                                             >  2:8100883/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:527321/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:6819369/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:4242646/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  2:320809/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCAGATACTCATTACAGGTGTTAATCATTATTTTGGCCGCTGCTTGTAGCACAGAAAGCATTAActcag                                                                                                           >  2:9311521/1‑139 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTATTTACCGTGTCTTTTCTGATACTCATTACATGTGTTAATCATTTTTTTGGCCGCTTCTTTTATCAAAGAAAGCATTAAatcaa                                                                                                           >  2:1461632/1‑139 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTACAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACATGTGTTAATCATTTTTTTGGCCGCATCATTTAGCAAAGAAAGCATTAAaacat                                                                                                           >  2:3376123/1‑140 (MQ=255)

                              gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          <  2:2186074/140‑1 (MQ=255)

                              gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  2:8604397/1‑140 (MQ=255)

                              gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  2:10309233/1‑140 (MQ=255)

                              gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  1:10581369/1‑140 (MQ=255)

                               aGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAAtcatca                                                                                                         >  2:3718159/1‑140 (MQ=255)

                                 tACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGc                                                                                                                              <  2:9755441/117‑1 (MQ=255)

                                    aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTTAATCATCATAATc                                                                                                    <  1:6070328/140‑1 (MQ=255)

                                    aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    <  2:158320/140‑1 (MQ=255)

                                    aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    >  1:7184395/1‑140 (MQ=255)

                                    aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    >  2:4565554/1‑140 (MQ=255)

                                     tGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCa                                                                                                   >  2:7249775/1‑140 (MQ=255)

                                        agagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                >  1:6440014/1‑140 (MQ=255)

                                        agagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                <  2:3030801/140‑1 (MQ=255)

                                         gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  2:1939388/1‑140 (MQ=255)

                                         gagaATAATCGCTTATCAGACGTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  1:2180683/1‑140 (MQ=255)

                                          agaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTa                                                                                              >  1:914983/1‑140 (MQ=255)

                                           gaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAg                                                                                             >  2:6330517/1‑140 (MQ=255)

                                           gaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAg                                                                                             >  2:441236/1‑140 (MQ=255)

                                            aataatCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGa                                                                                            >  1:10299511/1‑140 (MQ=255)

                                              taatCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAAt                                                                                          >  1:317242/1‑140 (MQ=255)

                                              taatCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAAt                                                                                          <  1:1695076/140‑1 (MQ=255)

                                                 tCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTg                                                                                       >  1:1887243/1‑140 (MQ=255)

                                                    cTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgtt                                                                                    >  1:8721599/1‑140 (MQ=255)

                                                    cTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgtt                                                                                    >  2:347960/1‑140 (MQ=255)

                                                    cTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgtt                                                                                    <  1:4934889/140‑1 (MQ=255)

                                                      tATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgttgt                                                                                  >  2:711852/1‑140 (MQ=255)

                                                      tATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgttgt                                                                                  >  1:747852/1‑140 (MQ=255)

                                                      tATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgttgt                                                                                  >  2:10914312/1‑140 (MQ=255)

                                                       aTCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgttgtt                                                                                 <  2:2377039/140‑1 (MQ=255)

                                                              ttCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGttt                                                                          <  2:4071811/140‑1 (MQ=255)

                                                               tCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTc                                                                         >  2:10095371/1‑140 (MQ=255)

                                                                      ccTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGttttt                                                                  <  1:6610369/140‑1 (MQ=255)

                                                                      ccTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGttttt                                                                  >  2:11259367/1‑140 (MQ=255)

                                                                        tCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTAt                                                                >  2:7419305/1‑140 (MQ=255)

                                                                        tCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTAt                                                                >  1:9409840/1‑140 (MQ=255)

                                                                        tCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTAt                                                                >  1:965260/1‑140 (MQ=255)

                                                                                tgtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGa                                                        >  2:9513168/1‑140 (MQ=255)

                                                                                tgtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGa                                                        >  1:8910143/1‑140 (MQ=255)

                                                                                 gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          <  2:11055039/89‑1 (MQ=255)

                                                                                 gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGag                                                       >  1:6267696/1‑139 (MQ=255)

                                                                                 gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  1:1430151/1‑140 (MQ=255)

                                                                                 gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  2:9143753/1‑140 (MQ=255)

                                                                                 gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGGTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  2:6885994/1‑140 (MQ=255)

                                                                                  tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  2:5126630/83‑1 (MQ=255)

                                                                                            gtgtCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTAttt                                            >  1:1172429/1‑140 (MQ=255)

                                                                                            gtgtCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTAttt                                            >  1:565113/1‑140 (MQ=255)

                                                                                            gtgtCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTAttt                                            >  1:7730311/1‑140 (MQ=255)

                                                                                              gtCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCg                                          >  1:10806487/1‑140 (MQ=255)

                                                                                               tCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCgg                                         >  2:6152207/1‑140 (MQ=255)

                                                                                                             cATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAAt                           >  1:301822/1‑140 (MQ=255)

                                                                                                              aTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       <  1:5105385/111‑1 (MQ=255)

                                                                                                              aTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAAtt                          >  1:11351494/1‑140 (MQ=255)

                                                                                                              aTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAAtt                          >  1:7008494/1‑140 (MQ=255)

                                                                                                                tACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTg                        >  1:3569214/1‑140 (MQ=255)

                                                                                                                   aGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcg                     <  1:7935833/140‑1 (MQ=255)

                                                                                                                     gtgtTAGTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   >  1:4736356/1‑140 (MQ=255)

                                                                                                                     gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAATCATTAAATAATCATACTCAACGTAGAATCTGTTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   >  2:7863722/1‑140 (MQ=255)

                                                                                                                      tgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCa                  <  2:2783359/140‑1 (MQ=255)

                                                                                                                        ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                >  1:5836806/1‑60 (MQ=255)

                                                                                                                        ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                <  2:5836806/60‑1 (MQ=255)

                                                                                                                        ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCAt                 <  2:5692921/139‑1 (MQ=255)

                                                                                                                        ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  2:6669688/1‑140 (MQ=255)

                                                                                                                        ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  1:8275410/1‑140 (MQ=255)

                                                                                                                        ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  1:3838846/1‑140 (MQ=255)

                                                                                                                          aaTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              >  2:10689221/1‑140 (MQ=255)

                                                                                                                          aaTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              >  2:2608295/1‑140 (MQ=255)

                                                                                                                          aaTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              >  2:10462691/1‑140 (MQ=255)

                                                                                                                           aTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGat             <  1:5477618/140‑1 (MQ=255)

                                                                                                                           aTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGat             <  1:3424229/140‑1 (MQ=255)

                                                                                                                            tCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGata            <  2:5998149/140‑1 (MQ=255)

                                                                                                                             cATTTTTTTGGCCGCATCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGatat           <  2:1756554/140‑1 (MQ=255)

                                                                                                                                  ttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTa      <  1:10002023/140‑1 (MQ=255)

                                                                                                                                     tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATTGATATCTTTATTc   <  1:9856165/140‑1 (MQ=255)

                                                                                                                                     tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTc   <  2:8781513/140‑1 (MQ=255)

TGTTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCTGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCG  >  NZ_CP025268/1236525‑1236798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`