BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,385,625	A→G	220 (TAA→TAG)	pgmB →	beta‑phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,385,625	0	A	G	99.5%	1926.4 / NA	556	220 (TAA→TAG)	pgmB	beta‑phosphoglucomutase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (258/294); minor base T (1/2); total (259/297)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 556 total aligned reads displayed.

GCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGC  >  NZ_CP025268/1385488‑1385761

 cGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCaa                                                                                                                                       <  2:1719055/140‑1 (MQ=255)

  gggCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCaaa                                                                                                                                      <  1:6371513/140‑1 (MQ=255)

  gggCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCaaa                                                                                                                                      >  1:1613822/1‑140 (MQ=255)

  gggCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCaaa                                                                                                                                      <  1:2601481/140‑1 (MQ=255)

     cATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGTCAAAACGTATAGCAAAGGa                                                                                                                                   <  1:845492/140‑1 (MQ=255)

     cATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGa                                                                                                                                   <  2:1178786/140‑1 (MQ=255)

       ttGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAAt                                                                                                                                 <  2:6595884/140‑1 (MQ=255)

          aCGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCaa                                                                                                                              <  2:2693361/140‑1 (MQ=255)

           cGCCATTAACTCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTAATGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAAAGTATAGCAAAGGAATAAAc                                                                                                                             >  2:3624013/1‑140 (MQ=255)

           cGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAAc                                                                                                                             <  1:10487594/140‑1 (MQ=255)

            gCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACa                                                                                                                            <  1:7589892/140‑1 (MQ=255)

            gCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACa                                                                                                                            <  1:7286666/140‑1 (MQ=255)

                ttAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCATTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAGCGTATAGCAAAGGAATCAACATGGc                                                                                                                        >  2:188831/1‑140 (MQ=255)

                 tAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCt                                                                                                                       <  2:7774561/140‑1 (MQ=255)

                 tAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCt                                                                                                                       <  1:8622264/140‑1 (MQ=255)

                 tAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCt                                                                                                                       <  1:4085446/140‑1 (MQ=255)

                 tAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCt                                                                                                                       <  2:2123877/140‑1 (MQ=255)

                    cGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATATCAAAGGAATCAACATGGCTCAg                                                                                                                    >  2:3897017/1‑140 (MQ=255)

                    cGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAg                                                                                                                    >  1:9141959/1‑140 (MQ=255)

                     gCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGACTCAACATGGCTCAGc                                                                                                                   >  1:9878746/1‑140 (MQ=255)

                      ccAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCt                                                                                                                  <  2:8812246/140‑1 (MQ=255)

                      ccAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCt                                                                                                                  <  2:1322359/140‑1 (MQ=255)

                      ccAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCt                                                                                                                  <  1:715836/140‑1 (MQ=255)

                      ccAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCt                                                                                                                  <  1:146054/140‑1 (MQ=255)

                        aGCGGGATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGAAATTTCTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGGATAGCAAAGGAAGCAAAATGGCTCAGCttt                                                                                                                >  2:2376331/1‑140 (MQ=255)

                         gCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTACCCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCACAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTc                                                                                                               >  2:7225571/1‑140 (MQ=255)

                          cGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCg                                                                                                              >  1:5686025/1‑140 (MQ=255)

                              aTGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTac                                                                                                          >  1:9698607/1‑140 (MQ=255)

                               tGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTaca                                                                                                         >  2:11307526/1‑140 (MQ=255)

                                     cGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACatat                                                                                                   >  1:10301701/1‑140 (MQ=255)

                                       gTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTc                                                                                                 >  1:9377923/1‑140 (MQ=255)

                                       gTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTc                                                                                                 >  1:8087869/1‑140 (MQ=255)

                                          gggATCGGCGCGGGCTTAAGCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCaaa                                                                                              <  2:9185960/140‑1 (MQ=255)

                                          gggATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCaaa                                                                                              <  1:3138008/140‑1 (MQ=255)

                                              tCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTAAGCTTTCGTTACAACATATTCAAAAAAt                                                                                          <  2:10374026/140‑1 (MQ=255)

                                                ggCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCt                                                                                        >  1:6544644/1‑140 (MQ=255)

                                                ggCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCt                                                                                        >  1:4107442/1‑140 (MQ=255)

                                                 gcgcGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCCGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGAAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTa                                                                                       <  1:10006488/140‑1 (MQ=255)

                                                  cgcgGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGACTCAACATGGCTCAGCTTGCGTTACAACATATTCAAAAAATCTAc                                                                                      >  1:7751019/1‑140 (MQ=255)

                                                   gcgGGCTTAACCGGGGCGCATTTACTGTTTCCTTCATCGGAATCACTCACCTGTCCGCGGTTATCGGCCTTCTTGCAATACGTATAGCAAAGGAATCATCATGGCTCAGCTTTCGTTACTACATTTTCAAAAAATCTACg                                                                                     <  2:5103022/140‑1 (MQ=255)

                                                   gcgGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTCTAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACg                                                                                     <  2:7979786/140‑1 (MQ=255)

                                                   gcgGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACg                                                                                     <  1:1145845/140‑1 (MQ=255)

                                                   gcgGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACg                                                                                     <  1:4315686/140‑1 (MQ=255)

                                                    cgGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGa                                                                                    >  1:5396689/1‑140 (MQ=255)

                                                     gggCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGAt                                                                                   <  1:8868233/140‑1 (MQ=255)

                                                     gggCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGAt                                                                                   <  1:4631788/140‑1 (MQ=255)

                                                     gggCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATAAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGAt                                                                                   <  2:4650079/140‑1 (MQ=255)

                                                      ggCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATa                                                                                  >  2:10204047/1‑140 (MQ=255)

                                                        cTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAAc                                                                                <  1:7093277/140‑1 (MQ=255)

                                                          tAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCa                                                                              <  2:5656450/140‑1 (MQ=255)

                                                          tAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCa                                                                              <  2:3788383/140‑1 (MQ=255)

                                                            acaggggCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCTGCATTCTGGCAAAACGTATAGCATAGGAATCAACATGGCTCAGATTTCGTTACAAGATATTCAAAAAATCTACGATAACCAgg                                                                            <  2:10486753/137‑1 (MQ=255)

                                                            aCCGGGGCGCACTTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTCTCGGCCTTCTGGCAAAACGTATAGCAAAGGACTCAACATGGGTCAGCTTTCGTTTCAACNTAGTCGAAAATTCTAGGATAACCAgg                                                                            >  1:6927864/1‑140 (MQ=255)

                                                            aCCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAATCGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAgg                                                                            >  2:573280/1‑140 (MQ=255)

                                                            aCCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAgg                                                                            <  2:6990540/140‑1 (MQ=255)

                                                            aCCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAgg                                                                            >  2:10776112/1‑140 (MQ=255)

                                                            aCCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAgg                                                                            >  1:7469794/1‑140 (MQ=255)

                                                               ggggCGCATTTACTGTTGCCTTCAACGTAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGc                                                                         <  2:4335173/140‑1 (MQ=255)

                                                                 ggCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCa                                                                              >  2:4024044/1‑133 (MQ=255)

                                                                  gcgcAATTACTGTTGCCTTCACCGGAAGCACTCACCTGGCCGCGGTTAACGCCCTTCTGGCAAAACGAATATCAAAGGAATCAACCTGGCTCAGCTTTCGTTACCACATATTCAACAAATCTACGATAACCAGGTGCaac                                                                      <  2:8564600/140‑3 (MQ=255)

                                                                  gcgcAATTACTGTTGCCTTCAACGGACTCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCAtg                                                                      <  2:6078382/140‑1 (MQ=255)

                                                                  gcgcAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCAtg                                                                      >  2:4460738/1‑140 (MQ=255)

                                                                  gcgcAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCAtg                                                                      <  1:8560482/140‑1 (MQ=255)

                                                                        ttACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGa                                                                <  2:1503189/140‑1 (MQ=255)

                                                                         tACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGaa                                                               <  1:11114657/140‑1 (MQ=255)

                                                                          aCTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAAACTACGAGAACCAGGTGCATGTGGTGAAg                                                              <  2:2938077/140‑1 (MQ=255)

                                                                           cTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGTAgg                                                             >  2:7629317/1‑140 (MQ=255)

                                                                               tGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACtt                                                         <  1:10805176/140‑1 (MQ=255)

                                                                                gCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTc                                                        <  1:1255787/140‑1 (MQ=255)

                                                                                gCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTc                                                        >  1:5992364/1‑140 (MQ=255)

                                                                                gCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTc                                                        >  1:5644359/1‑140 (MQ=255)

                                                                                cccTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAACGGAAACAACATGGCTCAGCTTTCGTTACCCCCTACTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTc                                                        <  2:11035294/139‑1 (MQ=255)

                                                                                  cTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATATACGATAACCAGGTGCTTGTGGTGAAGGACTTCaa                                                      >  2:6730851/1‑140 (MQ=255)

                                                                                   ttCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAAc                                                     >  1:4416681/1‑140 (MQ=255)

                                                                                   ttCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAAc                                                     <  1:4885921/140‑1 (MQ=255)

                                                                                   ttCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAAc                                                     <  1:1816503/140‑1 (MQ=255)

                                                                                   ttCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAAc                                                     <  1:3370707/140‑1 (MQ=255)

                                                                                     cAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTa                                                                                       <  1:2310420/104‑1 (MQ=255)

                                                                                      aaCGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCAtg                                                                      >  1:9519154/1‑120 (MQ=255)

                                                                                        cGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACCTGGCTCAGCTTTCGTTACAACATAGTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCACCCTGGa                                                >  2:6395663/1‑140 (MQ=255)

                                                                                           aaTCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAAt                                             <  1:10151260/140‑1 (MQ=255)

                                                                                           aaTCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAAt                                             >  2:5253074/1‑140 (MQ=255)

                                                                                           aaTCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAAt                                             >  2:3448209/1‑140 (MQ=255)

                                                                                           aaTCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAAt                                             >  2:10060083/1‑140 (MQ=255)

                                                                                             tcactcacCTGGCCGCGGTTATCGGCCTTCTGTCAAAACGTATAGCTAAGGTATCAACATGGCTCATCTTTCGTTACAACATATTCAAAATTTCTACGATAACCTGGTTCATGTGGTGAAGGACTTCATCCTGGAAATTg                                           <  2:9581842/140‑1 (MQ=255)

                                                                                             tcactcacCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTg                                           <  2:9275801/140‑1 (MQ=255)

                                                                                              cactcacCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGc                                          <  1:2659645/140‑1 (MQ=255)

                                                                                                ctcacCTGGCCGCGGTTATCGGCCTTCTGTCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCg                                        >  2:7419140/1‑140 (MQ=255)

                                                                                                  cacCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGAt                                      <  2:8999449/140‑1 (MQ=255)

                                                                                                    ccTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACCTGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATaa                                    <  1:5181542/140‑1 (MQ=255)

                                                                                                     cTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATaaa                                   <  1:2069616/140‑1 (MQ=255)

                                                                                                         ccGCGGTTATCGGCCTTCTGGCAAAACGGATAGCAAAGGAATCAACAGGGCTCAGCTTTCGTTACAACATAGTCAACAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGt                               >  2:3284644/1‑140 (MQ=255)

                                                                                                             ggTTATCGGCCTTCTGGCAAAACGTATCGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATGCAAAAAATCTAAGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGAGAAAGAGTTcgt                           >  2:3146987/1‑138 (MQ=255)

                                                                                                             ggTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCAt                           >  2:8418079/1‑140 (MQ=255)

                                                                                                             ggTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCAt                           >  2:5377971/1‑140 (MQ=255)

                                                                                                               ttATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCg                         >  1:3936286/1‑140 (MQ=255)

                                                                                                                       cTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTc                 >  2:2550907/1‑140 (MQ=255)

                                                                                                                        ttCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCg                <  1:290327/140‑1 (MQ=255)

                                                                                                                                  aCGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCggg      <  1:3649954/140‑1 (MQ=255)

                                                                                                                                  aCGTATAGCAAAGGAATCAACATGGCTCAGCTCTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCggg      <  1:4787572/140‑1 (MQ=255)

GCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGCTGC  >  NZ_CP025268/1385488‑1385761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`