BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,496,208	G→A	R163K (AGG→AAG)	cybB →	cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,496,208	0	G	A	99.2%	1314.6 / NA	375	R163K (AGG→AAG)	cybB	cytochrome b561
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (80/290); minor base T (0/3); total (81/294)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 377 total aligned reads displayed.

GACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTATTTG  >  NZ_CP025268/1496071‑1496344

gACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAagg                                                                                                                                        >  1:2741166/1‑140 (MQ=255)

 aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                       <  1:4210211/140‑1 (MQ=255)

 aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                       <  2:10426338/140‑1 (MQ=255)

    aTGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCTCTTTTTATGGAAGGacaa                                                                                                                                    <  2:11106507/140‑1 (MQ=255)

      gCCTTACGCTTCAGAGGCCTATTTCGTACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  2:8957039/140‑1 (MQ=255)

      gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  1:8821927/140‑1 (MQ=255)

      gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  1:9164484/140‑1 (MQ=255)

      gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  1:11476875/140‑1 (MQ=255)

      gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  2:1269062/140‑1 (MQ=255)

        cTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATCGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAccaca                                                                                                                                <  2:2639331/140‑1 (MQ=255)

         ttACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacacac                                                                                                                               <  1:964903/140‑1 (MQ=255)

             gCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCt                                                                                                                           <  2:10466471/140‑1 (MQ=255)

             gCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCt                                                                                                                           <  1:4649310/140‑1 (MQ=255)

             gCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCt                                                                                                                           <  1:10375784/140‑1 (MQ=255)

             gCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGCTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCt                                                                                                                           <  1:10279316/140‑1 (MQ=255)

               ttCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTAc                                                                                                                         <  1:10614245/140‑1 (MQ=255)

                      gCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaa                                                                                                                                    <  1:1785939/122‑1 (MQ=255)

                      gCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgat                                                                                                                  <  2:10216815/140‑1 (MQ=255)

                      gCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgat                                                                                                                  >  1:5725659/1‑140 (MQ=255)

                       ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                                 <  1:6238116/140‑1 (MQ=255)

                       ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                                 >  2:8797376/1‑140 (MQ=255)

                       ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACCGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                                 <  2:10731301/140‑1 (MQ=255)

                         aaTTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGcc                                                                                                               >  2:5438327/1‑140 (MQ=255)

                         aaTTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGcc                                                                                                               >  2:3637969/1‑140 (MQ=255)

                           tttCGATCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCcgc                                                                                                             <  1:7281238/140‑1 (MQ=255)

                                aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        <  1:11048418/140‑1 (MQ=255)

                                aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        <  1:8154354/140‑1 (MQ=255)

                                   gggTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGt                                                                                                     >  2:1147582/1‑140 (MQ=255)

                                       aGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCt                                                                                                 <  2:2253913/140‑1 (MQ=255)

                                           aGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGGTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAg                                                                                             <  2:1792065/140‑1 (MQ=255)

                                               tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGata                                                                                         >  2:8233357/1‑140 (MQ=255)

                                                   gTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTa                                                                                     <  1:3533982/140‑1 (MQ=255)

                                                   gTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTa                                                                                     <  1:4011901/140‑1 (MQ=255)

                                                   gTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTa                                                                                     <  1:320517/140‑1 (MQ=255)

                                                   gTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTa                                                                                     <  2:2088916/140‑1 (MQ=255)

                                                        ggCATGAAACTCGTGCGAATCGGGGATTATTTGTCAAAGTTTTTCAAGCTGCTGCTGCACACTCACACCTCTATTTCTTTCATGACATCTCACTTTTACGCTTGTTTCCTCTTAATCGTTCCTGAAGGATATTTagtgaa                                                                                <  2:1984920/140‑6 (MQ=255)

                                                        ggCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTACTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGaa                                                                                <  2:5502529/140‑1 (MQ=255)

                                                          cATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGaaaa                                                                              <  2:5082944/140‑1 (MQ=255)

                                                              aaaCGCTGGCGAATCTGGGATATTTTGTCATGGGGTTGCACGCTGCGGCTGAACTGGCAAACCACTATTTCTGGATGGACAACACACTTCTACGCTTGATGCCGCGTAAACGGTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:3637969/140‑1 (MQ=255)

                                                              aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:8361316/140‑1 (MQ=255)

                                                              aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:6451702/140‑1 (MQ=255)

                                                              aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:6775051/140‑1 (MQ=255)

                                                              aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGAACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:2126952/140‑1 (MQ=255)

                                                              aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGATGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:9429694/140‑1 (MQ=255)

                                                                   cTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTAc                                                                     <  2:8410445/140‑1 (MQ=255)

                                                                     ggCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          >  1:7745637/1‑133 (MQ=255)

                                                                          aTCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAAcc                                                              <  1:10045412/140‑1 (MQ=255)

                                                                           tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                             <  2:2972215/140‑1 (MQ=255)

                                                                             tGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAc                                                           <  1:6833377/140‑1 (MQ=255)

                                                                              gggATATTTTGTCATCGGGTTGCTCGCTGCGGCTGCACTGGCACACCAATATTTATGGAAGGACAACACACTTCTACGAATGATGCAGCTTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc                                                          <  2:8586844/140‑1 (MQ=255)

                                                                              gggATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGACGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc                                                          <  1:478419/140‑1 (MQ=255)

                                                                              gggATATTTTGTCATCGGGTGGCACGCGGCGGCTGCACTGGCACACCAATATTTCGGCAAGGACAACACACTTCTACGCATGATGCCGCGAAACCGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc                                                          <  1:7151139/140‑1 (MQ=255)

                                                                                gctatTTTGTCATCGGGTGGCACGCTGCGGCGGCACTGGCACACCCCTATTTCTGGAAGGACACCCCACTTCTCCGCAGGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAACACGCCTGTACTAAacccgacccg                                                        <  1:2571405/138‑1 (MQ=255)

                                                                                gatttgttGTCATCGTGTTGCACGCTGATGCTGCACGGGCACACCACGATTTATGGAAGGACAACACACTTCTAAGAATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGACAACGCCTGTACTCAAACCGACCCg                                                        <  2:1910264/134‑1 (MQ=255)

                                                                                gATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCg                                                        <  1:7826794/140‑1 (MQ=255)

                                                                                     tttGTCATGGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCGGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  1:90383/140‑1 (MQ=255)

                                                                                     tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  2:118047/140‑1 (MQ=255)

                                                                                     tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  1:1929767/140‑1 (MQ=255)

                                                                                     tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   >  2:3932867/1‑140 (MQ=255)

                                                                                     tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  2:9574705/140‑1 (MQ=255)

                                                                                     tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  1:8492445/140‑1 (MQ=255)

                                                                                     tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  2:7895977/140‑1 (MQ=255)

                                                                                     tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTATACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  2:3132648/140‑1 (MQ=255)

                                                                                     ttggTCATCGGGTTGCACGCGGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCAGGATGCCGCGTAACCGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  2:11033611/137‑1 (MQ=255)

                                                                                       tGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACa                                                 >  1:9974316/1‑140 (MQ=255)

                                                                                       tGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTAAATCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCGGAAGGAGATTTAAAGAAAACGCCTGTTCTAAAACCGCCCCGTGGTAcc                                                 >  2:1628575/1‑139 (MQ=255)

                                                                                           aTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                             <  2:2707626/140‑1 (MQ=255)

                                                                                           aTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGAATGATGCCGCGTAAACGTTCCTGATGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                             <  1:3034299/140‑1 (MQ=255)

                                                                                             ggggTTGCACGGTGGGGCTGCAGTGGCACACCACTATTTCTGGAAGGACAACACTCTTCTACGCATGATGCCGCGTAAACTTTCCTGAAGTTTATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCgaa                                           <  1:1247904/139‑1 (MQ=255)

                                                                                             cGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCgaa                                           <  2:5113217/140‑1 (MQ=255)

                                                                                                  tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCTTGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:2674684/140‑1 (MQ=255)

                                                                                                  tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGACGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:1528297/140‑1 (MQ=255)

                                                                                                  tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  2:7001555/140‑1 (MQ=255)

                                                                                                  tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:8635573/140‑1 (MQ=255)

                                                                                                  tGCACGCTGCGGCTGCACTGGCACACCACTATGTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  2:7691339/140‑1 (MQ=255)

                                                                                                   gCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGACTAc                                     <  2:1837238/140‑1 (MQ=255)

                                                                                                     aCGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGAATGATGCCGCGTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACgg                                   <  1:10743585/140‑1 (MQ=255)

                                                                                                        cTGCGGCTGCACTGGCACACGAATATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAATACGCCTGTACTATAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  2:6159496/140‑1 (MQ=255)

                                                                                                        cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAATACGCCTGTACTAAATCAGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  2:4085874/140‑1 (MQ=255)

                                                                                                        cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  2:9215187/140‑1 (MQ=255)

                                                                                                        cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  2:6864647/140‑1 (MQ=255)

                                                                                                        cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  1:10102207/140‑1 (MQ=255)

                                                                                                        cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGAAAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  2:3287665/140‑1 (MQ=255)

                                                                                                               tGCACTGTAACAACAATATGTATGGAAGGAAAACACAGTTCTACGATTGTTGCCGCGTAAACGTTCCTTAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCg                         <  1:5372173/140‑1 (MQ=255)

                                                                                                                 cACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGa                       <  2:5895936/140‑1 (MQ=255)

                                                                                                                 cACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGa                       >  2:3770424/1‑140 (MQ=255)

                                                                                                                 cACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGa                       >  1:5441614/1‑140 (MQ=255)

                                                                                                                   cTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAg                     >  1:5491663/1‑140 (MQ=255)

                                                                                                                    tGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgc                    <  1:11388187/140‑1 (MQ=255)

                                                                                                                     ggCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcg                   >  2:9300249/1‑140 (MQ=255)

                                                                                                                      gCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc                  >  1:6587034/1‑140 (MQ=255)

                                                                                                                      gCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc                  <  1:1407204/140‑1 (MQ=255)

                                                                                                                           ccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCGGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCtatt             <  1:3487820/140‑1 (MQ=255)

                                                                                                                           ccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAATTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGTAAGCGCCtatt             <  2:4838938/140‑1 (MQ=255)

                                                                                                                              cTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATtata          >  2:9656161/1‑140 (MQ=255)

                                                                                                                                 tttCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                             <  2:7225864/86‑1 (MQ=255)

                                                                                                                                      ggAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTTTAGAAAACGCCTGTACTAATACCGACCCGTGGTACAGGCGAAGATTACGGGTCGACATCGGACGCGCCTATTATATTTATTTg  >  2:453169/1‑140 (MQ=255)

                                                                                                                                      ggAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          >  1:2126627/1‑68 (MQ=255)

                                                                                                                                      ggAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCtattat           >  1:5079812/1‑131 (MQ=255)

                                                                                                                                      ggAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTATTTg  >  1:10448925/1‑140 (MQ=255)

GACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTATTTG  >  NZ_CP025268/1496071‑1496344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`