BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,761,825	A→G	T437A (ACT→GCT)	pykF →	pyruvate kinase PykF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,761,825	0	A	G	99.5%	2192.3 / NA	630	T437A (ACT→GCT)	pykF	pyruvate kinase PykF
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (176/451); minor base T (0/3); total (176/454)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 630 total aligned reads displayed.

TCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAA  >  NZ_CP025268/1761688‑1761961

tCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGct                                                                                                                                        >  1:10129135/1‑140 (MQ=255)

tCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGct                                                                                                                                        >  1:9300102/1‑140 (MQ=255)

tCCCGGAGGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTACTACCGTCTGGGTAAAGAACTGGct                                                                                                                                        <  1:6762197/140‑1 (MQ=255)

     gATGCCACCATCCTGGCTCTGACCACCACCGAACAACCGGCTCATCAGTTGGTACTGAGCATAGGCGTTGTGCCGCAGCTTGTTCAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa                                                                                                                                   <  2:10441351/140‑1 (MQ=255)

      aTGCCACCATCCTGGCACTGACCACCACCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACGGGCTCTGCag                                                                                                                                  <  1:3535924/140‑1 (MQ=255)

        gCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCagag                                                                                                                                <  2:6218612/140‑1 (MQ=255)

        gCCACCATCCGGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCagag                                                                                                                                <  2:7051751/140‑1 (MQ=255)

         ccaccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGc                                                                                                                               >  2:7493089/1‑140 (MQ=255)

          caccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCg                                                                                                                              >  1:374940/1‑140 (MQ=255)

            ccaTCCTGGCACTGACCACCCACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGAAGCGTGTTAAAGAGATCACTTCGACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt                                                                                                                            <  2:9221342/140‑1 (MQ=255)

            ccaGCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt                                                                                                                            <  1:5593059/140‑1 (MQ=255)

               tCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGTACTGGCTCTGCAGAGCGGTCTg                                                                                                                         <  1:10343512/140‑1 (MQ=255)

               tCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTg                                                                                                                         <  2:3055266/140‑1 (MQ=255)

                  tGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGca                                                                                                                      >  1:6187405/1‑140 (MQ=255)

                       cTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACaa                                                                                                                 >  2:10776449/1‑140 (MQ=255)

                        tGACCACCAACGAAAACACGGCTCAACAGTTGGTACTGAGCAAAGGCGTTGAGCCGCCGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGcacacaca                                                                                                                <  2:8894400/140‑3 (MQ=255)

                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg                                                                                                              >  2:3275282/1‑140 (MQ=255)

                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg                                                                                                              >  2:4607974/1‑140 (MQ=255)

                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAgg                                                                                                              >  1:2548662/1‑140 (MQ=255)

                             accaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                                           <  1:7320247/140‑1 (MQ=255)

                              ccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTGAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGTGTAAAGAACTGGCTCTGCCGAGCGGTCTGGCACACAATGGTGag                                                                                                          >  2:7654027/1‑139 (MQ=255)

                               caccGAAAACACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTGCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACg                                                                                                         <  1:10828086/137‑1 (MQ=255)

                                aaCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                                        <  2:2705310/140‑1 (MQ=255)

                                aaCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                                        <  2:10593437/140‑1 (MQ=255)

                                  cGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                                                                                                      >  2:4809289/1‑140 (MQ=255)

                                  cGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                                                                                                      <  1:50759/140‑1 (MQ=255)

                                    aaaaaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTa                                                                                                    >  2:7775120/1‑140 (MQ=255)

                                       aaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGtt                                                                                                 <  1:1159604/140‑1 (MQ=255)

                                         aCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGAAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTAt                                                                                               <  2:5846344/140‑1 (MQ=255)

                                            gCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGt                                                                                            <  2:6496908/140‑1 (MQ=255)

                                            gCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGt                                                                                            <  2:101872/140‑1 (MQ=255)

                                            gCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGt                                                                                            <  1:4877706/140‑1 (MQ=255)

                                               catcaGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTc                                                                                         <  2:5130767/140‑1 (MQ=255)

                                                atcaGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCt                                                                                        >  2:11329931/1‑140 (MQ=255)

                                                  caGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAATGTGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCTGTCTGGCACACAACGGTGACGTTGTAGTTATGGTTTCTgg                                                                                      <  1:4646684/140‑1 (MQ=255)

                                                  caGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                      <  1:1732626/140‑1 (MQ=255)

                                                  caGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                      <  2:8134450/140‑1 (MQ=255)

                                                  caGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                      <  1:958327/140‑1 (MQ=255)

                                                  caGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                      <  2:5201224/140‑1 (MQ=255)

                                                  caGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                      <  1:8323068/140‑1 (MQ=255)

                                                  caGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTAACGTCTGGGTAAAGAACTGTCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                      <  2:9667694/140‑1 (MQ=255)

                                                  caGTTGGTACTGAGCAAAGGCGTTGAGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCGGGCACACAAAGGTGACGTTGGAGTTATGGTTTCTgg                                                                                      <  2:8431443/140‑1 (MQ=255)

                                                      tGGTACTGCGCAAAGGCGTTGTGCCGCAGCATGATAAAGAGAGCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAACGGTGACGTTGTAGTTATGGTTTCTGGTGCa                                                                                  <  2:11422361/140‑1 (MQ=255)

                                                       ggTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCAc                                                                                 >  1:5922606/1‑140 (MQ=255)

                                                          aCTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGtt                                                                                                 <  1:2870750/121‑1 (MQ=255)

                                                              aGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTa                                                                                                    >  1:9086980/1‑114 (MQ=255)

                                                              aGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTa                                                                                                    <  2:9086979/114‑1 (MQ=255)

                                                                 acaGGCGTTGAGCCGCAGCTGGTTAAAGAGATCACGTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAACGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAg                                                                       <  2:6059407/138‑1 (MQ=255)

                                                                 aaaGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAg                                                                       <  1:4434486/140‑1 (MQ=255)

                                                                   aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt                                                                                                                            >  1:11172350/1‑85 (MQ=255)

                                                                   aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCg                                                                     >  2:2878267/1‑140 (MQ=255)

                                                                     gcctcgtgCCGCAGCTTGTCAACGAGATCACTTCTACAGCTGATGACTACCGTCTGGGTAAAGAACGGGCTCTGCAGAGCGGTCTGGCACACAACGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                                   <  1:11113739/135‑1 (MQ=255)

                                                                     gCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                                   <  1:3206441/140‑1 (MQ=255)

                                                                     gCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                                   <  1:4323865/140‑1 (MQ=255)

                                                                     gCGTTGTGCCGCAGCTTGTTAAAGAGATCACGTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTCCCGAGCGGc                                                                   <  2:6824160/140‑1 (MQ=255)

                                                                           tgCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactact                                                             <  2:4247006/140‑1 (MQ=255)

                                                                           tgCCGCAGCTTGTTAAAGAGATCACTTCGACTGATGATTTCTACCGTCTGGGTAAAGCACTGGCTCTGCAGAGCGGTCTGGCACACAACGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactact                                                             <  2:2442260/140‑1 (MQ=255)

                                                                             ccGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTaa                                                           <  1:6487395/140‑1 (MQ=255)

                                                                             ccGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTaa                                                           <  1:2944035/140‑1 (MQ=255)

                                                                             ccGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTaa                                                           <  2:3469391/140‑1 (MQ=255)

                                                                                cAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAacac                                                        <  2:4135734/140‑1 (MQ=255)

                                                                                cAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAacac                                                        <  2:382207/140‑1 (MQ=255)

                                                                                cAGCTTGTGACAGAGATCACTTCTACTGCTGATTGCGACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGGAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAacac                                                        <  2:7220229/140‑1 (MQ=255)

                                                                                  gCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCg                                                      <  2:4478071/140‑1 (MQ=255)

                                                                                     tgtgAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTCGCACACAAAGGTGCCGTTGTAGTTATGGTTTCTGGTGCACGGGTACCGAGCGGCACTACTAACACCGCAt                                                   <  2:5440499/140‑1 (MQ=255)

                                                                                     tGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCAt                                                   <  2:3946347/140‑1 (MQ=255)

                                                                                     tGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCAt                                                   <  1:7833670/140‑1 (MQ=255)

                                                                                     tGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCAt                                                   <  2:535019/140‑1 (MQ=255)

                                                                                     tGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCAt                                                   >  1:197401/1‑140 (MQ=255)

                                                                                     tGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCAt                                                   >  1:11383494/1‑140 (MQ=255)

                                                                                     cgctAAAGAGACCACTTCTACTGATGCATACTACCGTCCGGGTAAAGAACTCGCTCTGCCGAGCGGTCTGGCACACAAAGGTGACGTACGAGTCATGGCTACTGCAGCACAGGTACCGAGCGGCACTACAAACACCGCaa                                                   <  2:4980757/137‑2 (MQ=255)

                                                                                      gTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATc                                                  <  1:2429169/140‑1 (MQ=255)

                                                                                      gTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATc                                                  <  1:7449178/140‑1 (MQ=255)

                                                                                      gTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATc                                                  <  1:8852075/140‑1 (MQ=255)

                                                                                           agagATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTc                                             <  2:5573216/140‑1 (MQ=255)

                                                                                           agagATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTc                                             <  1:7996869/140‑1 (MQ=255)

                                                                                            gagaTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCa                                            <  1:533253/140‑1 (MQ=255)

                                                                                            gagaTCACTTCTACGGATGATTGCTCCCGTCAGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCa                                            <  2:3171852/140‑1 (MQ=255)

                                                                                             agaTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGt                                                                                            <  1:1564679/91‑1 (MQ=255)

                                                                                              gctCACTTCTACTGATGATTTCTACCGTCGGGGTAAAGACCTGGCTCTGCAGAGCGGTCAGGCCCACAACGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                          <  2:2198485/138‑1 (MQ=255)

                                                                                              gaTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                          <  1:10001908/140‑1 (MQ=255)

                                                                                              gaTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                          <  1:7664803/140‑1 (MQ=255)

                                                                                              gaTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                          <  1:2174716/140‑1 (MQ=255)

                                                                                              gaTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                          <  2:9392278/140‑1 (MQ=255)

                                                                                                tCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTc                                        <  1:8054487/140‑1 (MQ=255)

                                                                                                    ttCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGt                                    <  2:11068304/140‑1 (MQ=255)

                                                                                                       tACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                                      <  2:3614612/87‑1 (MQ=255)

                                                                                                       tACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAat                                 >  1:1395698/1‑140 (MQ=255)

                                                                                                        aCTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAata                                <  2:7946260/140‑1 (MQ=255)

                                                                                                        aCTGATGATTTCTACCGCCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTNCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAata                                <  1:6111556/140‑1 (MQ=255)

                                                                                                            atgatTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGc                            <  1:2063579/140‑1 (MQ=255)

                                                                                                                          cTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaat              <  1:10455415/140‑1 (MQ=255)

                                                                                                                          cTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaat              <  1:8571860/140‑1 (MQ=255)

                                                                                                                           tGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaatt             >  2:1273185/1‑140 (MQ=255)

                                                                                                                           tGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaatt             >  2:1743365/1‑140 (MQ=255)

                                                                                                                            gggTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAAttt            <  1:6924100/140‑1 (MQ=255)

                                                                                                                               tAAAGAACTGGCTCTGCAGAGCGGTCGGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGta         <  1:7144706/140‑1 (MQ=255)

                                                                                                                                    aaCTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCg    <  1:2679176/140‑1 (MQ=255)

                                                                                                                                      cTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGaa  <  1:991478/140‑1 (MQ=255)

                                                                                                                                      cTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGaa  <  1:3993831/140‑1 (MQ=255)

TCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAA  >  NZ_CP025268/1761688‑1761961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`