BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,957,477	A→G	132 (TAA→TAG)	yecN →	MAPEG family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,957,477	0	A	G	99.8%	1964.5 / NA	563	132 (TAA→TAG)	yecN	MAPEG family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (279/283); minor base C (0/1); total (279/284)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 563 total aligned reads displayed.

ATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATC  >  NZ_CP025268/1957340‑1957613

aTGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCg                                                                                                                                        <  2:570802/140‑1 (MQ=255)

aTGCATTATTACGGTGTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCg                                                                                                                                        <  2:8642140/140‑1 (MQ=255)

          aCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGc                                                                                                                              >  2:646360/1‑140 (MQ=255)

           cGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGcc                                                                                                                             >  2:2559532/1‑140 (MQ=255)

             gTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAc                                                                                                                           >  2:5417035/1‑140 (MQ=255)

             gTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAc                                                                                                                           >  1:6372407/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATCTGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAcgtt                                                                                                                        >  1:3370679/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                        >  2:1956821/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                        >  1:9072167/1‑140 (MQ=255)

                         gTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTccc                                                                                                               <  2:1293607/140‑1 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                              >  1:8835558/1‑140 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                              <  1:1624256/140‑1 (MQ=255)

                            tGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                            <  1:8609045/140‑1 (MQ=255)

                            tGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTGTCCCGGa                                                                                                            >  2:8871209/1‑140 (MQ=255)

                                ccGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGAtttt                                                                                                        <  2:5903315/140‑1 (MQ=255)

                                  gCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTa                                                                                                      >  1:94281/1‑140 (MQ=255)

                                    tGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACg                                                                                                    <  1:11104333/140‑1 (MQ=255)

                                    tGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACg                                                                                                    <  2:4501608/140‑1 (MQ=255)

                                    tGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACg                                                                                                    <  1:4546566/140‑1 (MQ=255)

                                      gCGACGATCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGTTGCTGGCGAATCTTTGGTATATGCACTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACATTCTGTTTCCCGGAATTTTACGtt                                                                                                  >  2:9206632/1‑140 (MQ=255)

                                                   aTGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACcgcg                                                                                     <  1:8739194/140‑1 (MQ=255)

                                                      aGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGaca                                                                                  >  2:6257781/1‑140 (MQ=255)

                                                          ccACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTGTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                              <  1:7313648/140‑1 (MQ=255)

                                                           cACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTCTATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATGTTTACGTTATGTCTCACCGCGACACGCTa                                                                             >  2:314258/1‑140 (MQ=255)

                                                                  tgtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                      <  1:6929855/140‑1 (MQ=255)

                                                                    tgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCATTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcc                                                                    <  2:11379237/140‑1 (MQ=255)

                                                                     gcgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGccc                                                                   >  2:10652317/1‑140 (MQ=255)

                                                                     gcgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGccc                                                                   <  1:8375298/140‑1 (MQ=255)

                                                                     gcgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGccc                                                                   >  2:6690536/1‑140 (MQ=255)

                                                                      cgcTGTTGGTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGAAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  >  1:6139698/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:815474/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  >  2:1079240/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  2:10087814/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:9322192/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:1008626/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:6583517/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:5344870/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  2:3589867/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:2811164/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  >  2:7932019/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:1206228/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTCAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:5513210/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTCGGAGTTGGTTTTCTCCCTGATATAGCGCACAATACCCCACTTTCTTTTACCCGGATTTTTACGTTATGTCACACCGCGACACGCTATTATCTGcccc                                                                  >  2:2301990/1‑140 (MQ=255)

                                                                           ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                             <  2:3131589/140‑1 (MQ=255)

                                                                           ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                             <  2:4892153/140‑1 (MQ=255)

                                                                           ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                             >  1:8863996/1‑140 (MQ=255)

                                                                           ttGCTGATGGGGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                             <  2:7450630/140‑1 (MQ=255)

                                                                              cTGATGGTGCTGGCGACTCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCa                                                          >  2:9779074/1‑140 (MQ=255)

                                                                              cTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCa                                                          >  2:3498489/1‑140 (MQ=255)

                                                                                gATGGTGCTGGCGAATCTTGGGTATAGGCCCTGGGAGTTGGTGTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTGTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGa                                                        <  2:9852255/140‑1 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                     >  1:4864549/1‑140 (MQ=255)

                                                                                    gTGCTGGCGACTCAGTGGTATCTGCCCTGGGAGTTTGATTTCTCCCTGCGTTCGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTgg                                                    <  2:7178332/140‑1 (MQ=255)

                                                                                    gTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATAAGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGAATCGCTATATTCTGCCCCTATCGCCAGACTgg                                                    <  1:5041013/140‑1 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCCCTTTCTTTGTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                   >  1:10765818/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                   >  1:10506523/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                   >  1:9934382/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                   >  1:4496370/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                   >  2:3420079/1‑140 (MQ=255)

                                                                                      gCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGc                                                  >  2:6505605/1‑140 (MQ=255)

                                                                                       cTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCACGGATTTTTACGTTATGACTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGTCg                                                 >  2:506357/1‑140 (MQ=255)

                                                                                           cGAATCTTTGGTATATGCCCTGGGAGTTGGTATTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTGTCCCCGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                             <  2:4076959/140‑1 (MQ=255)

                                                                                              aTCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAc                                          <  2:1676135/140‑1 (MQ=255)

                                                                                              aTCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAc                                          <  1:3565296/140‑1 (MQ=255)

                                                                                              aTCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAc                                          <  1:2353676/140‑1 (MQ=255)

                                                                                               tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAcc                                         <  1:3124799/140‑1 (MQ=255)

                                                                                               tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAcc                                         >  2:10417851/1‑140 (MQ=255)

                                                                                                 tttGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCtt                                       <  1:198368/140‑1 (MQ=255)

                                                                                                    ggTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtga                                    >  2:2973828/1‑140 (MQ=255)

                                                                                                         atGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCTCTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCATATCGCAAGACTGTGCGACTGGACCGTTGTTGAAc                               >  2:11071544/1‑140 (MQ=255)

                                                                                                         atGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAAc                               >  1:2648681/1‑140 (MQ=255)

                                                                                                          tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTa                                                                             >  2:7000091/1‑93 (MQ=255)

                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACgg                             >  1:10318402/1‑140 (MQ=255)

                                                                                                            cccTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                            <  2:4765027/140‑1 (MQ=255)

                                                                                                            cccTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                            <  2:940156/140‑1 (MQ=255)

                                                                                                             ccTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                              <  2:8399193/89‑1 (MQ=255)

                                                                                                               tGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAg                         >  1:9238848/1‑140 (MQ=255)

                                                                                                               tGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGAGTAg                         >  1:9831587/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  1:412438/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  2:388435/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                        >  1:2893352/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCAAGACTGGGCGACTGGACCTATGATGAACGGGTAGc                        >  1:8037487/1‑140 (MQ=255)

                                                                                                                    gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                    <  1:9686812/140‑1 (MQ=255)

                                                                                                                    gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                    <  1:2155148/140‑1 (MQ=255)

                                                                                                                    gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                    >  1:5738659/1‑140 (MQ=255)

                                                                                                                    gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACGTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                    <  1:8433002/140‑1 (MQ=255)

                                                                                                                     ttGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAg                   <  1:7139791/140‑1 (MQ=255)

                                                                                                                      tGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt                  <  2:10884669/140‑1 (MQ=255)

                                                                                                                      tGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt                  <  1:1405141/140‑1 (MQ=255)

                                                                                                                      tGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt                  >  1:11293838/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                  <  1:3912267/91‑1 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                 <  1:5470504/140‑1 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                 >  2:8537177/1‑140 (MQ=255)

                                                                                                                           ttCTCCCTGCGTTAGCGCACAATACGCCCCTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATGTTCTGCCCCTATCGCCAGACTGGGCGACTGGAGCGTTGATGAACGGGTAGCTGAAGTCTTcc             >  2:610266/1‑140 (MQ=255)

                                                                                                                            tctcCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc            <  1:4673897/140‑1 (MQ=255)

                                                                                                                               cccTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGa         >  1:4330896/1‑140 (MQ=255)

                                                                                                                               cccTGCGGTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTCTTTTCTGCCCCTATCGCCAGAATGGGAGACTGGACCTGTGATGAACGGGTAGCTGAAGTCTTCACGGa         >  2:7742427/1‑140 (MQ=255)

                                                                                                                                ccagCGTTAGCGCACAATACGCCACGTCCTTTGTCCCGGAATATTACGTTATGACACACCGCGACACGCTATATTCTGCCCCTATCGCCAGACAGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGACTTCCCGGat        <  1:7836179/137‑1 (MQ=255)

                                                                                                                                 cTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata       <  1:7570318/140‑1 (MQ=255)

                                                                                                                                 cTGCGTTAGCGCACAATACGCCACTGTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata       <  1:4790676/140‑1 (MQ=255)

                                                                                                                                   gCGTTAGCGCACAATACTCCACTTTCTTTCTCCCGGATTGTTACGATCTGTCTCACCGCGACACGCTATTTTCTGCCCCTAACGCCAGACTGGGCGACTGCACCTCTGATGAACGGGTAGCTGACGTCTTCCCGGATATg     <  2:9629712/140‑1 (MQ=255)

ATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGATC  >  NZ_CP025268/1957340‑1957613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`