BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,002,534	T→C	329 (TAA→TAG)	dcyD ←	D‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,002,534	0	T	C	99.7%	2417.7 / NA	698	329 (TAA→TAG)	dcyD	D‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (287/409); minor base A (1/1); total (288/410)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 698 total aligned reads displayed.

CGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT  >  NZ_CP025268/2002397‑2002670

ctcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAAGAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTa                                                                                                                                        <  2:9024525/138‑1 (MQ=255)

cgcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTa                                                                                                                                        >  1:11242323/1‑140 (MQ=255)

cgcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAGCAATAACCAGTTGGATACTTTCTTGCATGCGCGGTTCCGGAGCTa                                                                                                                                        >  2:9311650/1‑140 (MQ=255)

  cgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTaaa                                                                                                                                      >  2:2946145/1‑140 (MQ=255)

   gagaATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAAc                                                                                                                                     <  1:11087063/140‑1 (MQ=255)

       aTAACCCCGAGCAGTAAGCCAGAAATCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTAAGCAGGTACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTGCCGGAGCTAAACgggg                                                                                                                                 >  2:180494/1‑140 (MQ=255)

        tAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTggg                                                                                                                                <  1:2059655/140‑1 (MQ=255)

           aCCCGAGCAGTAAGCCAAAAAACATGCCGCCGAGACTGAGTTGCAGCGTATACCCAGCCCCTGTCAGCAGGAACGGCAAGGAATCCCGAACCAGGTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat                                                                                                                             <  2:1383254/140‑1 (MQ=255)

               gAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATa                                                                                                                         >  1:2952996/1‑140 (MQ=255)

                 gCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGACTCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAgg                                                                                                                       >  1:7616589/1‑140 (MQ=255)

                   aGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCg                                                                                                                     <  1:118483/140‑1 (MQ=255)

                    gTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGa                                                                                                                    >  1:3908415/1‑140 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                                   <  2:6531867/140‑1 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                                   <  1:2852119/140‑1 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                                   >  1:3022297/1‑140 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGAAGACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                                   <  2:5414059/140‑1 (MQ=255)

                        gCCAAACTACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCGTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAg                                                                                                                <  2:6178658/140‑1 (MQ=255)

                                    gccgccGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAcc                                                                                                    <  2:317314/140‑1 (MQ=255)

                                     ccgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                                   <  2:172986/140‑1 (MQ=255)

                                     ccgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                                   <  1:2528245/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  2:52502/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTATCAGCACGAACGGCAAGGAATCACTAACCAGTGGAATACGATCGTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  2:8122000/140‑1 (MQ=255)

                                              cTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAAGAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGa                                                                                          <  2:5612498/140‑1 (MQ=255)

                                               tGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGaa                                                                                         <  1:9932522/140‑1 (MQ=255)

                                               tGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGaa                                                                                         >  2:960100/1‑140 (MQ=255)

                                               tGAGTTGCAGCGTATACCCAGCCCCGTTCATCAGGAACGGCAAGGAATCTCTAACCAGTTGTATACTTTATTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATTaa                                                                                         <  2:7757178/140‑1 (MQ=255)

                                                  gTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATaa                                                                                      <  1:519718/140‑1 (MQ=255)

                                                      cAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAg                                                                                  >  1:4658402/1‑140 (MQ=255)

                                                          gTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATc                                                                              <  1:1805039/140‑1 (MQ=255)

                                                           tataCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCg                                                                             >  1:10567839/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGACTCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:1046384/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  2:1592747/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:7420347/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCAGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:42534/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTAGTATACTTTCTTGCATGAGCGTTTCCTGAGCTAAACGTGGGGATGAAAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:4837702/1‑140 (MQ=255)

                                                                   gCCCCGTTCAGCAGGACCGGCAAGGAATCAATAACCAGTTGTATACTTTCGTGCAGGTGCGTGTCCGGAGCGAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTGCa                                                                     >  2:1247463/1‑140 (MQ=255)

                                                                   gCCCCGTTCAGCAGGAACGGCAAGGAATCAATAACCAGGTGTAGCCGTTCTTGCATGAGCGTTTCCGGAGCTAATGGTGGGGATGATAGGCGAACCGCGCAGGCGCACCGCCGGTCTGACGACACAGAATCGGCCCGTcc                                                                     >  2:1965635/1‑139 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                    >  1:6004404/1‑140 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGAGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCCGTATGAATAAACAGAATCGGCCCTTCAt                                                                    >  2:6347900/1‑140 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                                   >  1:7151720/1‑140 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                                   >  1:4970295/1‑140 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                                   >  2:442374/1‑140 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                                   >  2:3346738/1‑140 (MQ=255)

                                                                      ccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCt                                                                  >  1:5682637/1‑140 (MQ=255)

                                                                           cagcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGaa                                                             <  1:6397767/140‑1 (MQ=255)

                                                                           cagcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGaa                                                             <  2:11242322/140‑1 (MQ=255)

                                                                            agcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               >  1:8569620/1‑105 (MQ=255)

                                                                            agcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAg                                                            <  2:4642806/140‑1 (MQ=255)

                                                                              cagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCg                                                          >  2:3956803/1‑140 (MQ=255)

                                                                              cagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCg                                                          >  2:4347426/1‑140 (MQ=255)

                                                                                ggAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGtt                                                        >  2:3788770/1‑140 (MQ=255)

                                                                                 gAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                       <  1:9629846/140‑1 (MQ=255)

                                                                                 gAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                       <  1:10307154/140‑1 (MQ=255)

                                                                                         aGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTctgactga                                               <  1:8146462/140‑1 (MQ=255)

                                                                                         aGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTctgactga                                               <  1:6506935/140‑1 (MQ=255)

                                                                                                   caCCAGGTGAATACTTTCTTGCAAGAGCGTTTCCGGAGCTAAACGTGGGGATGAAAGGCGAACAGCGCAGGAGCACCGCCGGTATGAAAAAACAGAATCCGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAAt                                     <  2:6864573/139‑1 (MQ=255)

                                                                                                    aCCAGTTGTATACTTTCTTGCATGAGCGTGTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATc                                    <  1:1463377/140‑1 (MQ=255)

                                                                                                     ccAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCa                                   >  1:3661455/1‑140 (MQ=255)

                                                                                                        gTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                                <  1:912834/140‑1 (MQ=255)

                                                                                                         ttGGATACTTTCTTGCGTGAGCGTTTCCGGAGCTATACGTGGGGATGATCGGCGAACAGCGCAGGCGCACCGCCGGGCGGAATAAACCGACTCGGCCGTTCATCTGTGAAGCGGTTCTGACTGTTACCGTCATGCAGccc                               >  2:2340565/1‑140 (MQ=255)

                                                                                                            tataCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGTTAGGCGAACAGCGCAGGCGCAACGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGATTCTGACTGATACCGTAAATCAGCCCCGc                            >  2:5832599/1‑140 (MQ=255)

                                                                                                            tataCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGc                            >  1:5863537/1‑140 (MQ=255)

                                                                                                             ataCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc                           <  2:24244/140‑1 (MQ=255)

                                                                                                              taCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCa                          >  2:7319206/1‑140 (MQ=255)

                                                                                                              taCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCa                          >  2:8800839/1‑140 (MQ=255)

                                                                                                               aCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCAt                         <  1:10176876/140‑1 (MQ=255)

                                                                                                                 tttCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCg                       <  2:2113364/140‑1 (MQ=255)

                                                                                                                 tttCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCg                       >  2:7210227/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAAc                                                                                                                  <  1:3538684/47‑1 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  2:3543886/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  2:8606258/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  1:343647/1‑140 (MQ=255)

                                                                                                                    cTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtt                    <  1:7925287/140‑1 (MQ=255)

                                                                                                                    cTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtt                    <  1:5397763/140‑1 (MQ=255)

                                                                                                                    cTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtt                    <  1:8328772/140‑1 (MQ=255)

                                                                                                                    cTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtt                    <  1:1329162/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  <  1:6732567/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  >  2:848717/1‑140 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  >  2:11110578/1‑140 (MQ=255)

                                                                                                                       gCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTc                 <  1:9437246/140‑1 (MQ=255)

                                                                                                                         aTGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGACTCGGCCCCTCATCTTTGAAGCGTTTCTGACTGATACCGTCACTCAGCCCCGCCATCGCATTTCCg               <  2:6049600/140‑1 (MQ=255)

                                                                                                                         aTGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCg               <  1:5523446/140‑1 (MQ=255)

                                                                                                                         aTGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCg               <  2:10936183/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCTTACAGCGCAGGCGCCCCGCCGGTAGGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCATCCACGCCATCGCATTTCCGGt             <  2:7093762/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGTTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCCTCTCTTTTCAGGt             >  2:4722531/1‑140 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  >  1:2352994/1‑135 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  2:101537/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  1:442374/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  1:7802686/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             >  2:4963244/1‑140 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATAGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  2:10580509/140‑1 (MQ=255)

                                                                                                                            aGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGta            <  1:6267344/140‑1 (MQ=255)

                                                                                                                                tttCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca        <  1:1084954/140‑1 (MQ=255)

                                                                                                                                tttCCGGAGCTAAACGTGGGGATGAGAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTGTCCGGGATaca        <  2:4194915/140‑1 (MQ=255)

                                                                                                                                  tCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca      >  1:10011989/1‑140 (MQ=255)

                                                                                                                                  tCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca      <  2:10297974/140‑1 (MQ=255)

                                                                                                                                  tCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca      <  1:1603100/140‑1 (MQ=255)

                                                                                                                                  tCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca      <  2:3598755/140‑1 (MQ=255)

                                                                                                                                      gAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAt  >  1:172652/1‑140 (MQ=255)

                                                                                                                                      gAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAt  <  2:5032515/140‑1 (MQ=255)

CGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT  >  NZ_CP025268/2002397‑2002670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`