BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,154,002	T→G	G341G (GGA→GGC)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,154,002	0	T	G	98.3%	2002.3 / NA	585	G341G (GGA→GGC)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand): ref base T (1/9); new base G (192/383); total (193/392)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 606 total aligned reads displayed.

CCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTA  >  NZ_CP025268/2153865‑2154138

ccTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGcc                                                                                                                                        <  2:4679247/140‑1 (MQ=255)

   aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                     <  2:1771798/140‑1 (MQ=255)

   aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                     >  1:1622248/1‑140 (MQ=255)

   aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                     <  1:5346662/140‑1 (MQ=255)

    aaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGTCTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgaga                                                                                                                                    >  2:1277482/1‑140 (MQ=255)

    aaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgaga                                                                                                                                    >  1:10784024/1‑140 (MQ=255)

    aaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgaga                                                                                                                                    >  1:1100442/1‑140 (MQ=255)

     aGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                   <  2:66415/140‑1 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                               >  2:2076900/1‑140 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                               >  1:1006304/1‑140 (MQ=255)

          gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTCCTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                              >  2:7441023/1‑140 (MQ=255)

          gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                              <  1:2076900/140‑1 (MQ=255)

            ggCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGATACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa                                                                                                                            <  1:9636040/140‑1 (MQ=255)

             gCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATaaaa                                                                                                                           >  1:7373195/1‑140 (MQ=255)

                  ctaGTCGGTTGGATTTGATCGGTTAATGGGTGTTTCTTTGCTGGCTGTGCTTCTGTGGACATCTCCATCGCCATGAATTTCGCCAGCACAAAATTCCCGCCGGTGGTTTTGTCCTCTTCGCCGAGATCAATAAAATAGAt                                                                                                                      <  2:8484943/138‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCTCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  2:7002345/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:4961550/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:1054777/140‑1 (MQ=255)

                            ggAAATGTTCGTGTAATGGCTGTTGCATTGCTGGCGGTTCTTCTGTCGCCATCACCATCGCCCAGACTTTCGCGAGCACAATTTTCCCGCCGGTGGTAATGACCTCTTCGTCGAGTTCAATAAAATAGATAACACCTTGa                                                                                                            <  2:1020926/140‑1 (MQ=255)

                            ggAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGTTCAATAAAATAGTTAACACCTTgg                                                                                                            >  1:6165172/1‑139 (MQ=255)

                            ggAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGa                                                                                                            <  2:8160064/140‑1 (MQ=255)

                               aaTGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACACAATTCCCGCCGGTGGTAATTACCTCTTCGCCGAGATCAAGATAAAAGATAACACCTTGATTa                                                                                                         >  2:9005333/1‑140 (MQ=255)

                                aTGATCTGGTAATGTATGTTGCAATGCTGGCGGTGATACTGACGACATCTGCTTCGCCCAGACTTTCGCCTGCTCAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                        <  2:1937390/140‑1 (MQ=255)

                                aTGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                        <  1:9274393/140‑1 (MQ=255)

                                aTGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCAACATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                        <  2:9259368/140‑1 (MQ=255)

                                 tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                       <  1:69237/140‑1 (MQ=255)

                                  gATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATcc                                                                                                      >  2:5669018/1‑140 (MQ=255)

                                   aTCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCa                                                                                                     >  1:339318/1‑140 (MQ=255)

                                    tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCACAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACATCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAg                                                                                                    >  2:1659062/1‑140 (MQ=255)

                                      gggTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCTCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTTATTATCCAgct                                                                                                  <  1:645935/140‑2 (MQ=255)

                                      gggTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcg                                                                                                  <  1:2784684/140‑1 (MQ=255)

                                      gggTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcg                                                                                                  <  2:8572262/140‑1 (MQ=255)

                                         tAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcac                                                                                               <  2:4195749/140‑1 (MQ=255)

                                           aTGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCacac                                                                                             <  2:1006272/140‑1 (MQ=255)

                                                  ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                      >  2:4427563/1‑140 (MQ=255)

                                                  ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                      >  2:11008822/1‑140 (MQ=255)

                                                  ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                      >  2:5197965/1‑140 (MQ=255)

                                                  ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                      >  2:9784471/1‑140 (MQ=255)

                                                  ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATACAGCGCACACCACGGGc                                                                                      >  1:5650598/1‑140 (MQ=255)

                                                    gCATTGCTGGCGGTGCTACTGACGACATCACCATCGCCCATACTTTCGCCAGCACATTTTTCCCGACGGTGGTAATGACGTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                    <  2:10775662/140‑1 (MQ=255)

                                                    gCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCACAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATTGATAACACCTTGATTATCCAGCGCACCCCACGGGcag                                                                                    <  2:1440982/140‑1 (MQ=255)

                                                        tGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagtcag                                                                                <  1:4599115/140‑1 (MQ=255)

                                                            ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaa                                                                            >  1:10449951/1‑140 (MQ=255)

                                                            ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaa                                                                            >  2:7726205/1‑140 (MQ=255)

                                                                gTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAACTGa                                                                        <  2:3012630/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCTCCATCGCCCAGACTTTCGCAAGCACAAAATTCCCGCCGGTGGTTATGACCTCTTCGCCGAGATCAATAATATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  2:3765680/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:1841136/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:7031932/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAGAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  2:7134812/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAAGTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:8476553/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACGTTCGCCATCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGACAAATGAg                                                                       <  2:2257471/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGAATTTCGCCAGCACAATATTCCCGCCTGTGGTATTGACCTATTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCTCGGGCAGTCAGTCAAATGAg                                                                       <  2:3374200/140‑1 (MQ=255)

                                                                  gCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       >  2:7251846/1‑139 (MQ=255)

                                                                  gCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGc                                                                      >  1:8746109/1‑140 (MQ=255)

                                                                   cTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCg                                                                     >  1:11352414/1‑140 (MQ=255)

                                                                    tACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCgg                                                                    <  2:2795192/140‑1 (MQ=255)

                                                                    tACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCgg                                                                    <  1:4037637/140‑1 (MQ=255)

                                                                      cTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCAGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCgggg                                                                  <  2:3909181/140‑1 (MQ=255)

                                                                       tGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGt                                                                 >  1:7474954/1‑140 (MQ=255)

                                                                         aCGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTaa                                                               >  2:9104425/1‑140 (MQ=255)

                                                                           gCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAAc                                                             >  1:230207/1‑140 (MQ=255)

                                                                           gCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAAc                                                             <  2:11042130/140‑1 (MQ=255)

                                                                           gCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAAc                                                             >  1:3751104/1‑140 (MQ=255)

                                                                             cATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  2:2336617/128‑1 (MQ=255)

                                                                               tCACCATCGCCCAGACTTTCGCCAGCACAACATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATCACACCTTGATGATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGc                                                         <  1:11134962/140‑1 (MQ=255)

                                                                               tCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGc                                                         <  1:4821775/140‑1 (MQ=255)

                                                                               tCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGc                                                         <  1:5877177/140‑1 (MQ=255)

                                                                                cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCTAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                        >  1:6595650/1‑140 (MQ=255)

                                                                                cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                        >  1:7225391/1‑140 (MQ=255)

                                                                                cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                        <  1:9056309/140‑1 (MQ=255)

                                                                                    aTCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGc                                                    <  2:6321184/140‑1 (MQ=255)

                                                                                               tttCGCCATCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc                                         <  2:3219009/140‑1 (MQ=255)

                                                                                               tttCGCCAGCACAAAATTCCCGCCGGTGGTTATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCATGATTATCCATCGCACACCAAGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATGGCTGAAACGATAc                                         <  2:4907536/140‑1 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                                       <  1:4395388/140‑1 (MQ=255)

                                                                                                  cGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcg                                                                                                  <  2:958312/80‑1 (MQ=255)

                                                                                                     cAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCt                                   <  1:8257406/140‑1 (MQ=255)

                                                                                                       gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCttt                                 >  1:7663854/1‑140 (MQ=255)

                                                                                                       gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCttt                                 <  1:5188953/140‑1 (MQ=255)

                                                                                                          caAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAAc                              <  2:834558/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:452253/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:3509153/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:251780/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:9520307/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:4315245/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAAGAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:7957324/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCAGGTGGTAATGACCTCTTCGCCTAGATCAATAAAATATATAACAACTTTATTATCCAGCGCACTCCACTGTCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:1050259/140‑1 (MQ=255)

                                                                                                               ttCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTg                         <  1:1391004/140‑1 (MQ=255)

                                                                                                                 cccGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGc                       <  2:5392142/140‑1 (MQ=255)

                                                                                                                  ccgccgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCg                      >  2:5077512/1‑140 (MQ=255)

                                                                                                                  ccgccgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCg                      >  2:4069095/1‑140 (MQ=255)

                                                                                                                   cgccgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTATACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGt                     <  2:11336529/140‑1 (MQ=255)

                                                                                                                      cgGTGTTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGTTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                  <  2:7929879/140‑1 (MQ=255)

                                                                                                                      cgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                  <  2:5983319/140‑1 (MQ=255)

                                                                                                                                   ctctTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCg     >  1:9985432/1‑140 (MQ=255)

                                                                                                                                      ttcgCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                        >  1:3002664/1‑38 (MQ=255)

                                                                                                                                      ttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                    >  2:6710090/1‑58 (MQ=255)

                                                                                                                                      ttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                    <  1:6710090/58‑1 (MQ=255)

                                                                                                                                      ttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAACTGAGCGGGGTAATCATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGGGCCGCTa  >  2:8770244/1‑140 (MQ=255)

                                                                                                                                      ttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTa  >  2:7613290/1‑140 (MQ=255)

                                                                                                                                      ttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTa  >  1:3243358/1‑140 (MQ=255)

CCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTA  >  NZ_CP025268/2153865‑2154138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`