BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,183,367	C→T	S170S (AGC→AGT)	yegT →	nucleoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,183,367	0	C	T	99.2%	1900.3 / NA	499	S170S (AGC→AGT)	yegT	nucleoside permease
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (296/196); minor base G (2/2); total (300/199)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 527 total aligned reads displayed.

GTGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTC  >  NZ_CP025268/2183231‑2183502

gTGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttct                                                                                                                                      >  2:1327884/1‑140 (MQ=255)

 tGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctg                                                                                                                                     >  2:3138658/1‑140 (MQ=255)

 tGATTTCCCGCGCATTCGTGTGATGGGCAATATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCAGCGAGATACATGCCGATGCGGATTTCCGACGGTAggtctg                                                                                                                                     >  2:11428406/1‑140 (MQ=255)

  gATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctgc                                                                                                                                    <  2:2696589/140‑1 (MQ=255)

    tttCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctgctc                                                                                                                                  <  1:9803887/140‑1 (MQ=255)

        ccGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTctgctctgct                                                                                                                              <  1:8982301/140‑1 (MQ=255)

        ccGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTctgctctgct                                                                                                                              <  1:1297309/140‑1 (MQ=255)

        ccGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGACTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTctgctctgct                                                                                                                              <  1:10612404/140‑1 (MQ=255)

            gcATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGgt                                                                                                                          <  1:9500006/140‑1 (MQ=255)

                   tgtgATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTg                                                                                                                   <  2:9485050/140‑1 (MQ=255)

                      gATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGt                                                                                                                <  2:1117092/140‑1 (MQ=255)

                        tGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGACGTTCTGCTCTGCTCGGTGTGTTTGCGttt                                                                                                              >  2:10987340/1‑140 (MQ=255)

                        tGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGttt                                                                                                              >  1:271482/1‑140 (MQ=255)

                        tGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGgtt                                                                                                              >  1:7203296/1‑140 (MQ=255)

                          ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGttttt                                                                                                            >  1:8444830/1‑140 (MQ=255)

                          ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGttttt                                                                                                            >  1:9248583/1‑140 (MQ=255)

                          ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGttttt                                                                                                            >  1:2968012/1‑140 (MQ=255)

                          ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTAACGCCGGAAGTTCTGCTCTGCTCGGTGTGTGTGCGttttt                                                                                                            >  2:6304063/1‑140 (MQ=255)

                           gCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTc                                                                                                           <  1:5688401/140‑1 (MQ=255)

                           gCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTc                                                                                                           <  2:1534034/140‑1 (MQ=255)

                             aCTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCt                                                                                                         >  1:9130111/1‑140 (MQ=255)

                                    gCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGac                                                                                                  >  1:7640276/1‑140 (MQ=255)

                                    gCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGac                                                                                                  >  1:1370014/1‑140 (MQ=255)

                                    gCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGac                                                                                                  >  1:994041/1‑140 (MQ=255)

                                        gATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                              <  1:8062085/140‑1 (MQ=255)

                                         aTTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGcc                                                                                             >  1:3798352/1‑140 (MQ=255)

                                           tGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATCTCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGGGTTTGCGTTTTGCCTGCCCGACACGccac                                                                                           >  1:615186/1‑140 (MQ=255)

                                              cTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCaa                                                                                        >  1:4771593/1‑140 (MQ=255)

                                               tCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTGTGCGTTTTTCCTGCCCGACACGCCACCcaa                                                                                       >  1:11304586/1‑140 (MQ=255)

                                                  ggTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAg                                                                                    >  2:9265747/1‑140 (MQ=255)

                                                   gTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGc                                                                                   >  1:5142072/1‑140 (MQ=255)

                                                   gTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGc                                                                                   >  1:10825777/1‑140 (MQ=255)

                                                   gTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGc                                                                                   >  1:4801155/1‑140 (MQ=255)

                                                   gTCAGGCATGTCGATTCTTGCCTCAAATACTGGGGTATGCCGAAATCTCACCGACTAACAACACGCTGCTGATAAACGCCGGACGATCTGCTCAGCTCGCACTGTATGCGTTTGTCCGGCGCCCAACGCCACCAAAAAGc                                                                                   >  2:7423803/1‑140 (MQ=255)

                                                     cTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCAc                                                                                 <  1:10254413/140‑1 (MQ=255)

                                                     cTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCAc                                                                                 <  2:244853/140‑1 (MQ=255)

                                                              ggTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                        <  2:810050/140‑1 (MQ=255)

                                                              ggTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                        <  2:5467855/140‑1 (MQ=255)

                                                              ggTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCGGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                        <  1:8900980/140‑1 (MQ=255)

                                                               gTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATg                                                                       <  1:3100505/140‑1 (MQ=255)

                                                                 ttCTTGCCGCAAATACTGAGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTGTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGa                                                                     <  2:888704/140‑1 (MQ=255)

                                                                  tCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGat                                                                    >  1:702667/1‑140 (MQ=255)

                                                                      gCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTa                                                                >  1:871084/1‑140 (MQ=255)

                                                                          cAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGt                                                            <  2:9738023/140‑1 (MQ=255)

                                                                                cTGGGGTATGCCGCTATCTCACCGACTAACATCCCGCTGCTGATGACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                      <  2:7928836/140‑1 (MQ=255)

                                                                                cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGTAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCTTGCt                                                      <  1:5025987/140‑1 (MQ=255)

                                                                                cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                      <  1:45482/140‑1 (MQ=255)

                                                                                cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                      <  1:2163397/140‑1 (MQ=255)

                                                                                cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                      <  1:5952539/140‑1 (MQ=255)

                                                                                   gggTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCgg                                                   <  2:2296187/140‑1 (MQ=255)

                                                                                   gggAATGCCGATATCTCACCGACGAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCgg                                                   <  2:6669803/140‑1 (MQ=255)

                                                                                    ggTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGc                                                  >  2:10397302/1‑140 (MQ=255)

                                                                                     gTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                                 <  1:4025754/140‑1 (MQ=255)

                                                                                     gTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                                 <  1:1446795/140‑1 (MQ=255)

                                                                                        tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAATGTCATGCTCGGCCTgg                                              >  2:10849894/1‑140 (MQ=255)

                                                                                        tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                              <  1:10057586/140‑1 (MQ=255)

                                                                                        tGCCGATATCTCACCGACTAACATCCCGATGCTGATTACAGCAGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCAGACAAGCCACCAAAAAGCACAGGCAAAATGGATATTAATGTCATGATCGGCCTgg                                              >  2:7308588/1‑140 (MQ=255)

                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAACATGGATATTAAAGTCATGCTCGGCCTGGa                                             <  2:4808004/140‑1 (MQ=255)

                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                             >  2:4186525/1‑140 (MQ=255)

                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                             <  1:11002804/140‑1 (MQ=255)

                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                             >  1:790192/1‑140 (MQ=255)

                                                                                         gCCGATATCTCACCGACTAACATCCCGCTGCAGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTGTTTCCGGCCCGATCCGACACCAAAATGCTCCGGCGCACTGGATCTTACAGTCATGCTCGGCCTGGa                                             >  2:10010992/1‑140 (MQ=255)

                                                                                            gATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgc                                          <  2:5278102/140‑1 (MQ=255)

                                                                                             atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATg                                                        >  1:10003448/1‑125 (MQ=255)

                                                                                              tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcgc                                        <  2:6862835/140‑1 (MQ=255)

                                                                                              tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcgc                                        <  2:8597834/140‑1 (MQ=255)

                                                                                              tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcgc                                        <  2:2938164/140‑1 (MQ=255)

                                                                                              tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcgc                                        >  2:8699737/1‑140 (MQ=255)

                                                                                               atCTCTCCGACTAACATCCCGCTGCTGATTTCCGCCGGATGTTCGGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAGTTGGAGATTAAAGTCATGCTCGGCCTGGATGCGCt                                       >  2:6406346/1‑140 (MQ=255)

                                                                                               atCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                      >  1:3428926/1‑125 (MQ=255)

                                                                                                 ctcACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTCCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGa                                     <  1:1964163/140‑1 (MQ=255)

                                                                                                   cACCGACTAACATCCCGCTGCTGCTTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAACATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATc                                   <  2:5778221/140‑1 (MQ=255)

                                                                                                    aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc                                  >  2:905525/1‑140 (MQ=255)

                                                                                                    aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc                                  >  1:11467785/1‑140 (MQ=255)

                                                                                                    aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc                                  >  2:10649059/1‑140 (MQ=255)

                                                                                                    aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAGGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc                                  >  1:5405546/1‑140 (MQ=255)

                                                                                                     ccGACTAAGATCCGGCTGCTGATTACCGCCGGAAGTTCTGCTCTACTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCct                                 >  1:6136089/1‑140 (MQ=255)

                                                                                                        aCTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgct                              >  1:228906/1‑140 (MQ=255)

                                                                                                        aCTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgct                              >  2:8247372/1‑140 (MQ=255)

                                                                                                        aCTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGAGATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgct                              >  1:11131369/1‑140 (MQ=255)

                                                                                                          tAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAATCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCtgctgc                            <  2:501681/140‑1 (MQ=255)

                                                                                                           caCATCCCGCTGCTGAGTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCATGCCCGACACGCCACCAAAAAGCACCTGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCTCTGATCCTGCTgcg                           <  2:7624758/139‑1 (MQ=255)

                                                                                                            aCATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTgcgc                          >  2:4731561/1‑140 (MQ=255)

                                                                                                              aTCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGa                        >  2:10577912/1‑140 (MQ=255)

                                                                                                                  cGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATaaa                    >  1:2470188/1‑140 (MQ=255)

                                                                                                                  cGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCGCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAATATGGATAGTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATaaa                    >  2:6170915/1‑140 (MQ=255)

                                                                                                                   gctgcgGATGACCGCCGGAACGTCTGCACTGCTCGGTGTGCTTGCGAGGTACCTGCCCGCCACGCCACCAAAAAGCACCGGCACAATGGATAATAACGTCATGCTCGGCCTGGATGCGCTGCTCCTGCTGCGCGATaaaa                   <  2:10162197/140‑1 (MQ=255)

                                                                                                                    ctgctgATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATaaaaa                  >  2:4038487/1‑140 (MQ=255)

                                                                                                                       ctgATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCTAATTGGAGATTAAAGTCTTGCTCGGCCTGGCGGCGCTGAGCCTGCTGCGCGCTCAAAACtt               >  2:7150276/1‑140 (MQ=255)

                                                                                                                         gATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTcc             <  1:6479816/140‑1 (MQ=255)

                                                                                                                          aTTACCGCCGGCAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCGGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTccg            >  2:3389022/1‑139 (MQ=255)

                                                                                                                          aTTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCt            <  1:8229727/140‑1 (MQ=255)

                                                                                                                            tACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAACAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg          <  2:3709824/140‑1 (MQ=255)

                                                                                                                            tACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg          <  2:255709/140‑1 (MQ=255)

                                                                                                                            tACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg          <  1:656668/140‑1 (MQ=255)

                                                                                                                            tACCGCCGGAAGTTCTGCTCTGCTCGGTGTGATTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg          <  1:6498432/140‑1 (MQ=255)

                                                                                                                              ccgccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTc        >  1:4224284/1‑140 (MQ=255)

                                                                                                                              ccgccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTc        <  1:6789621/140‑1 (MQ=255)

                                                                                                                               cgccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTATAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCt       >  2:1842369/1‑140 (MQ=255)

                                                                                                                                 ccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCttt     >  1:5554274/1‑140 (MQ=255)

GTGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTC  >  NZ_CP025268/2183231‑2183502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`