BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,415,086	G→C	T101S (ACC→AGC)	yfbS ←	SLC13 family permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,415,086	0	G	C	99.7%	2232.9 / ‑5.1	646	T101S (ACC→AGC)	yfbS	SLC13 family permease
Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (318/325); minor base A (1/1); total (320/326)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 647 total aligned reads displayed.

TAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414949‑2415222

       cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:9766636/1‑140 (MQ=255)

       cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  2:10907634/1‑140 (MQ=255)

           gCACAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATcaacaa                                                                                                                             <  2:5009630/140‑1 (MQ=255)

            cAAAACTTAACGGCATCATCAGACGTTACGGCGACGTTTGCATAAGAATGGCAACGCTTAACACCACGGTAATAAAGATAGCGACAACGCCGGATGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACaaa                                                                                                                            >  2:1630239/1‑140 (MQ=255)

               aaCTTATCTGAATCATCAGACGTGACGGCGACTTTTGCATACGCATGGCAACGATTAACACAAAGGGAATAAATATAGCGACAAAGCCGTTTGAACTCATAAACGCGCCACGCCACGAGAAGCTCAGCATCAACAAAAcc                                                                                                                         <  2:3673492/140‑1 (MQ=255)

                 cTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCaa                                                                                                                       >  1:7809643/1‑140 (MQ=255)

                    aaCGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAt                                                                                                                    >  1:10113486/1‑140 (MQ=255)

                     aCGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAtt                                                                                                                   <  2:10409183/140‑1 (MQ=255)

                      cGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACAttt                                                                                                                  >  1:2104210/1‑140 (MQ=255)

                       ggCATCATCCGCCGAGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACACAACCAACAtttt                                                                                                                 <  2:4211811/140‑1 (MQ=255)

                         catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGa                                                                                                               >  2:1762241/1‑140 (MQ=255)

                         catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGa                                                                                                               >  2:2567098/1‑140 (MQ=255)

                         catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGa                                                                                                               >  1:2154413/1‑140 (MQ=255)

                           tcatcaGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGCCAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGAtt                                                                                                             <  2:8949925/140‑1 (MQ=255)

                           tcatcaGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGAtt                                                                                                             >  1:34582/1‑140 (MQ=255)

                                   cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATCGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCACCGCGACGCTCAGCCTCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  1:2448005/1‑140 (MQ=255)

                                   cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  2:10040256/1‑140 (MQ=255)

                                   cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGAATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  2:7185383/1‑140 (MQ=255)

                                   cGTGACGGCGACGGTTGCATACGCATGGCAACGCTTAACACCACGGGCATAAAGATAGCGACAACGACGGTTGAACTCATAAACGCGCCAAGCCCCGCGTCGCTCAGCATCAACAAAACCAACATTTTGATTTCGCTAtt                                                                                                     >  2:6690101/1‑140 (MQ=255)

                                     tGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATATTGATTTCACTATTGc                                                                                                   >  2:3314374/1‑140 (MQ=255)

                                       aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 >  1:3308448/1‑140 (MQ=255)

                                       aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 >  2:4627911/1‑140 (MQ=255)

                                        cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  1:230345/140‑1 (MQ=255)

                                        cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                >  1:3047927/1‑140 (MQ=255)

                                            gACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAAc                                                                                            >  1:2885078/1‑140 (MQ=255)

                                                  tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  1:5007946/140‑1 (MQ=255)

                                                  tGCATACGCATGGCAACGCATAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  1:8535361/140‑1 (MQ=255)

                                                       aCGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGcc                                                                                 <  2:4922389/140‑1 (MQ=255)

                                                        cGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCa                                                                                >  2:1840051/1‑140 (MQ=255)

                                                         gCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCAt                                                                               <  1:8074966/140‑1 (MQ=255)

                                                          cATGGCAACGCTTAACACCACGGGAATAACGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACCAAACCACCATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATg                                                                              <  1:7384628/140‑1 (MQ=255)

                                                           aTGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGt                                                                             >  2:10667787/1‑140 (MQ=255)

                                                           aTGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGt                                                                             <  1:11467357/140‑1 (MQ=255)

                                                             ggCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTc                                                                           >  2:8541680/1‑140 (MQ=255)

                                                                  cGCTTAACACCTCGTGAATAAAGATAGCGAAAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCTACATTATGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                      <  2:5117681/140‑1 (MQ=255)

                                                                  cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCagt                                                                      >  2:10188010/1‑138 (MQ=255)

                                                                   gCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTa                                                                     >  2:8408238/1‑140 (MQ=255)

                                                                     ttAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACg                                                                   <  1:5778029/140‑1 (MQ=255)

                                                                       aaCACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCACCTTTGACCAGCCATGTTCCCATTACGGt                                                                 >  2:2338563/1‑140 (MQ=255)

                                                                          cccacGGGAATAAAGAGAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:11286960/139‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCCGGTTCCCAGTACGGTGGc                                                              <  2:8755998/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  1:6711107/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  1:2812294/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:615014/140‑1 (MQ=255)

                                                                           ccacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCa                                                             <  1:6361074/140‑1 (MQ=255)

                                                                            cacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  2:3470202/1‑140 (MQ=255)

                                                                            cacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCAGCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  2:7514203/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  1:8776621/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  1:11174298/1‑140 (MQ=255)

                                                                              cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:1637068/1‑140 (MQ=255)

                                                                              cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  2:5299663/1‑140 (MQ=255)

                                                                              cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          <  1:3621466/140‑1 (MQ=255)

                                                                               gggAATAAAGATAGCGTCAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTTTTGCCCGCAACTTTGACCAGCCATGTTCCCTGTACGGTGGCAacac                                                         <  2:1147562/140‑1 (MQ=255)

                                                                               gggAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGcaccac                                                         >  2:567356/1‑140 (MQ=255)

                                                                               gggAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAacac                                                         <  1:4861602/140‑1 (MQ=255)

                                                                                 gAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCg                                                       >  1:7566525/1‑140 (MQ=255)

                                                                                   aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     <  1:419717/140‑1 (MQ=255)

                                                                                   aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTGTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     <  2:832429/140‑1 (MQ=255)

                                                                                    gaaaGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATGGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  2:303088/139‑1 (MQ=255)

                                                                                      aaGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACg                                                  >  2:3206738/1‑140 (MQ=255)

                                                                                         aTAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGAc                                               >  2:9230090/1‑140 (MQ=255)

                                                                                         aTAGCGACAACGCCGGATGAACTCATAAACGCGCCTAGCCCCGCGACGCTCAGCATCAACAACACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGAc                                               <  2:3800446/140‑1 (MQ=255)

                                                                                          tAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGAcc                                              <  1:175626/140‑1 (MQ=255)

                                                                                           aGCGACAANGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACNAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAATACGGTGGCAACACCGGTACGGACCa                                             >  1:6133911/1‑140 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAacac                                                         <  1:7064860/128‑1 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             <  1:5634594/140‑1 (MQ=255)

                                                                                             cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                           >  1:5296901/1‑140 (MQ=255)

                                                                                             cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                           >  1:10320545/1‑140 (MQ=255)

                                                                                             cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                           >  2:6522764/1‑140 (MQ=255)

                                                                                               aCAACGCCGTTTGAACTCATAAACGCGCCAAGCCCAGCGACGCTCAGCATAAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAAcc                                         <  2:7015011/140‑1 (MQ=255)

                                                                                                cAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAAcc                                         >  1:10182867/1‑139 (MQ=255)

                                                                                                    gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:5535750/140‑1 (MQ=255)

                                                                                                        gTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaat                                <  2:6015219/140‑1 (MQ=255)

                                                                                                         ttGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaata                               <  2:2097452/140‑1 (MQ=255)

                                                                                                          tGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaataa                              >  1:6005415/1‑140 (MQ=255)

                                                                                                          tGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaataa                              >  1:7708331/1‑140 (MQ=255)

                                                                                                             aCTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAAtaataa                           >  2:9756420/1‑140 (MQ=255)

                                                                                                                cATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACa                        <  2:141450/140‑1 (MQ=255)

                                                                                                                cATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACa                        >  1:5441653/1‑140 (MQ=255)

                                                                                                                     aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  2:1249350/140‑1 (MQ=255)

                                                                                                                     aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  2:7974688/140‑1 (MQ=255)

                                                                                                                     aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCAATATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  1:9438482/140‑1 (MQ=255)

                                                                                                                      cgcgCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcgg                  >  1:6570279/1‑140 (MQ=255)

                                                                                                                        cgcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCa                <  2:7353288/140‑1 (MQ=255)

                                                                                                                         gcCAAGCCGCGCGTCGCTCAGCATCAACAAAACCAAGAGTTTGAGTTCACTCTTGCCCGCAACTTTGACCAGCCATGTGGCCATGACGGTGGCAACTCCGGTCCGGACCAAACCATCGGCACTGATAGACAAGGCGGCag               >  2:4075810/1‑139 (MQ=255)

                                                                                                                         gcCAAGCCCCGGGACGATCAGCATCAACAAATCCAACATTTTGAATTCACTATAGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCTACAACGGTACGTACCAAACCATGGCCAATAATAAACTTggcggcta               >  2:7129660/1‑138 (MQ=255)

                                                                                                                         gcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACGAAACCATCGCCAATAATAAACAAGGCGGCaa               >  1:8195724/1‑140 (MQ=255)

                                                                                                                         gcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCaa               >  2:9594305/1‑140 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCACCAAACCCAACATTTTGATTTCACTATTGACAGAAACTTTGATCAGCCATGTTCCCTTTACGGTGGCAANACCGGTACGGACCAAACCATCGCCAATAAGAACCAAGGCGGCAATCAGGa         <  1:10626888/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACCTTTTGATTTCACTCTTGCCCGCAACTTTGACCAGCCATGGTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATTATAAACAAGGCGGCAATCAGGa         <  2:2755802/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:9181936/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:10742750/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:11035980/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:1386354/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:3984284/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:4602678/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:5836379/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:6901600/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTGTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCACTCAGGa         <  2:7646/140‑1 (MQ=255)

                                                                                                                                 ccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa       <  1:8934475/140‑1 (MQ=255)

TAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414949‑2415222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`