BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,654,514	T→C	M600V (ATG→GTG)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,654,514	0	T	C	99.9%	2339.9 / NA	669	M600V (ATG→GTG)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (359/309); minor base G (0/1); total (359/310)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 669 total aligned reads displayed.

GTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCATCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGTG  >  NZ_CP025268/2654377‑2654650

   aCTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGc                                                                                                                                     >  2:2883883/1‑140 (MQ=255)

      gCCTTCATCAACAATGGGCTGTTTCGCCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGAc                                                                                                                                  <  1:90711/140‑1 (MQ=255)

           catcaACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGccc                                                                                                                             >  1:4316241/1‑140 (MQ=255)

              caacaaTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGAt                                                                                                                          <  2:6084501/140‑1 (MQ=255)

              caacaaTGGGCTGTTTCACCGTGCCGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGAt                                                                                                                          <  2:1266374/140‑1 (MQ=255)

                  aaTGGGCTGTTTCACCGTGCTTTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCa                                                                                                                      <  2:312787/140‑1 (MQ=255)

                   aTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAg                                                                                                                     >  2:1421383/1‑140 (MQ=255)

                      ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATCGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                                  >  1:4994602/1‑140 (MQ=255)

                      ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                                  >  1:6200920/1‑140 (MQ=255)

                      ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                                  >  1:4676783/1‑140 (MQ=255)

                      ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                                  >  1:509586/1‑140 (MQ=255)

                      ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                                  >  1:6532066/1‑140 (MQ=255)

                       gCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaaa                                                                                                                 <  1:7214732/140‑1 (MQ=255)

                       gCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaaa                                                                                                                 <  1:10559907/140‑1 (MQ=255)

                       gCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaaa                                                                                                                 <  1:3959046/140‑1 (MQ=255)

                          gTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGCTTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTa                                                                                                              <  2:7598704/140‑1 (MQ=255)

                          gTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTa                                                                                                              <  1:1250118/140‑1 (MQ=255)

                           tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACNGGGCGACCGCCCGATTCCCGCAAACtcc                                                                                                             >  1:6095053/1‑138 (MQ=255)

                            ttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAcc                                                                                                            <  1:8561721/140‑1 (MQ=255)

                             tCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAccc                                                                                                           <  1:9710428/140‑1 (MQ=255)

                              cACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGAGACCGCCCGATTCCAGCAAACTACCCt                                                                                                          <  1:5685209/140‑1 (MQ=255)

                                 cGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGa                                                                                                       >  1:5842822/1‑140 (MQ=255)

                                   tGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGaaa                                                                                                     >  1:5530941/1‑140 (MQ=255)

                                    gcggTCAGTACGATAACCCAACACCGATTACGAGGCGCCCAGCGGACGGATCCCCGGCACTGCCTCGGCAGGCCAGATACCCTGCTCAGCGCGGCTCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGaaaa                                                                                                    <  2:10941196/137‑1 (MQ=255)

                                     cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   >  2:1118128/1‑140 (MQ=255)

                                     cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   <  1:9238060/140‑1 (MQ=255)

                                     cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   >  2:2660424/1‑140 (MQ=255)

                                     cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   >  1:1985710/1‑140 (MQ=255)

                                     cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   >  2:3923800/1‑140 (MQ=255)

                                     cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                                   >  1:10443170/1‑140 (MQ=255)

                                        tctgttcGATACTCGTAAACCGATTTCGAGGCGCACTGAGGACGGATCACCGGAATTGAATAGGCAGGCCAGATAGCCTGCTCAGCGCGTAGCGTCTCCGGGCGACCGCCCGATTCCAGCAATCTACCCTGGAAAATAAc                                                                                                <  2:9698095/134‑1 (MQ=255)

                                        tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                <  2:4251234/140‑1 (MQ=255)

                                        tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                <  2:51677/140‑1 (MQ=255)

                                        tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                <  1:10926726/140‑1 (MQ=255)

                                        tCAGTACGATAATCGTAAACCGATTTAGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                                <  1:9417/140‑1 (MQ=255)

                                          aGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCt                                                                                              >  1:3255840/1‑140 (MQ=255)

                                             aCGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTa                                                                                           <  1:3175636/140‑1 (MQ=255)

                                                  aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                      >  2:4080959/1‑140 (MQ=255)

                                                  aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                      >  2:3660486/1‑140 (MQ=255)

                                                  aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                      <  1:8906004/140‑1 (MQ=255)

                                                   aTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATgggg                                                                                     <  1:4520581/140‑1 (MQ=255)

                                                    tCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGa                                                                                    >  2:4608861/1‑140 (MQ=255)

                                                    tCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGa                                                                                    >  1:8203441/1‑140 (MQ=255)

                                                    tCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGa                                                                                    >  1:3609570/1‑140 (MQ=255)

                                                    tCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGa                                                                                    >  1:6342382/1‑140 (MQ=255)

                                                    acGTAAAACGATTTAGAGGCGAACTCCGGACGGATCACCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCTGCGCGACACAGGGCGACCGCCCGATTCCAGCACACTACCCTCAACAATAAACTGTAATGGGGa                                                                                    >  2:10107994/2‑140 (MQ=255)

                                                      gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTCATGGGGaag                                                                                  >  2:11314767/1‑139 (MQ=255)

                                                      gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                                  >  2:3033084/1‑140 (MQ=255)

                                                      gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                                  >  2:1511700/1‑140 (MQ=255)

                                                      gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                                  >  2:7346676/1‑140 (MQ=255)

                                                       tAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACGACCCTGGAAAATAACCTGTAATGGGGAATg                                                                                 <  1:5330641/140‑1 (MQ=255)

                                                          aCCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGt                                                                              >  1:343857/1‑140 (MQ=255)

                                                           ccGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGtt                                                                             >  2:6923820/1‑140 (MQ=255)

                                                              aTTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttcc                                                                          >  1:1751843/1‑140 (MQ=255)

                                                              aTTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttcc                                                                          <  1:2588085/140‑1 (MQ=255)

                                                                    aGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCAc                                                                    <  2:6811436/140‑1 (MQ=255)

                                                                        gAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGc                                                                <  1:2224325/140‑1 (MQ=255)

                                                                            tGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTc                                                            <  1:2146267/140‑1 (MQ=255)

                                                                                    gaTCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGaaa                                                    >  2:8817962/1‑140 (MQ=255)

                                                                                    gaTCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAACCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGaaa                                                    >  2:1840660/1‑140 (MQ=255)

                                                                                     aTCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAAc                                                   >  1:4801124/1‑140 (MQ=255)

                                                                                      tCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACt                                                  >  1:9572347/1‑140 (MQ=255)

                                                                                        cccGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTc                                                >  1:8040498/1‑140 (MQ=255)

                                                                                             cAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgccgc                                           >  1:1066081/1‑140 (MQ=255)

                                                                                              actGCATCGGCAGGCCAGCGAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                          <  1:10019456/138‑1 (MQ=255)

                                                                                              aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                          <  2:5715290/140‑1 (MQ=255)

                                                                                              aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                          <  1:747456/140‑1 (MQ=255)

                                                                                               aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCgcgc                                         >  1:10276011/1‑140 (MQ=255)

                                                                                               aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCgcgc                                         <  1:8386367/140‑1 (MQ=255)

                                                                                               aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCgcgc                                         >  1:3750665/1‑140 (MQ=255)

                                                                                                tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                        >  2:8059158/1‑140 (MQ=255)

                                                                                                tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                        <  2:556403/140‑1 (MQ=255)

                                                                                                  cATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCt                                      <  2:10723993/140‑1 (MQ=255)

                                                                                                      ggCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGAGACCGCCCGATTCCAGCAAACTACCCTGGAAAAGAACCTGTAATGGTGAATGCGTTTCCTTCCACTGGCTTTCTGGAGAAACTTCGCCGCGCCCTTTat                                  <  2:6516719/140‑1 (MQ=255)

                                                                                                         aGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCNCGCCCTTTatcat                               <  1:9844298/140‑1 (MQ=255)

                                                                                                         aGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTatcat                               <  2:8512951/140‑1 (MQ=255)

                                                                                                          ggCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTatcatc                              >  1:11252653/1‑140 (MQ=255)

                                                                                                            ccAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCa                            >  2:7840418/1‑140 (MQ=255)

                                                                                                              aGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGc                          <  2:6211411/140‑1 (MQ=255)

                                                                                                                aTAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGt                        <  2:3355209/140‑1 (MQ=255)

                                                                                                                    ccTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAAc                    >  2:2475311/1‑140 (MQ=255)

                                                                                                                     cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                   >  1:1868601/1‑140 (MQ=255)

                                                                                                                     cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt                   <  2:6005659/140‑1 (MQ=255)

                                                                                                                      tGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTg                  >  2:9205795/1‑140 (MQ=255)

                                                                                                                       gCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGa                 >  2:9385846/1‑140 (MQ=255)

                                                                                                                       gCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGa                 <  1:7047767/140‑1 (MQ=255)

                                                                                                                       gCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGa                 <  2:10270987/140‑1 (MQ=255)

                                                                                                                       gCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGa                 <  1:7571992/140‑1 (MQ=255)

                                                                                                                        cTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGaa                >  1:289339/1‑140 (MQ=255)

                                                                                                                         tCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAAc               >  1:8643182/1‑140 (MQ=255)

                                                                                                                         tCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAAc               >  2:8704802/1‑140 (MQ=255)

                                                                                                                             cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa           >  1:1585860/1‑140 (MQ=255)

                                                                                                                             cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa           >  2:3958173/1‑140 (MQ=255)

                                                                                                                             cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa           >  2:4567030/1‑140 (MQ=255)

                                                                                                                             cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa           >  2:2249441/1‑140 (MQ=255)

                                                                                                                             cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCAGCCAGCGTCAACTGAACTTCa           >  2:8213998/1‑140 (MQ=255)

                                                                                                                               cggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATc         <  2:10492157/140‑1 (MQ=255)

                                                                                                                                  cgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAg      >  1:11112554/1‑140 (MQ=255)

                                                                                                                                    cgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCg    >  2:4833093/1‑140 (MQ=255)

                                                                                                                                     gTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGt   >  1:6630580/1‑140 (MQ=255)

GTTACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCATCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAGCGTG  >  NZ_CP025268/2654377‑2654650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`