BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,938,222	A→G	S112G (AGC→GGC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,938,222	0	A	G	99.7%	2107.9 / NA	607	S112G (AGC→GGC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (331/273); minor base T (1/1); total (333/274)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 607 total aligned reads displayed.

TGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGA  >  NZ_CP025268/2938086‑2938358

tGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCa                                                                                                                                       <  1:7284170/140‑1 (MQ=255)

  taGCCGCTGCTTGCGTAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAACCCATCGACATGGATCCAACCCGCCCGAAGGCCCTGTGGGGCTTGAACGGCACt                                                                                                                                     >  2:1845626/2‑140 (MQ=255)

   aGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCGTTAACGGCACTg                                                                                                                                    >  1:5100952/1‑140 (MQ=255)

     gctctgctTGCGAAGAATAATTCAGCAGTCAGATTTTAGGCCTCACCATTACGGTATCGACTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCAAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGaa                                                                                                                                  <  2:11444964/137‑1 (MQ=255)

     ccGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGaa                                                                                                                                  <  2:2567552/140‑1 (MQ=255)

     ccGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGaa                                                                                                                                  <  1:3351984/140‑1 (MQ=255)

     ccGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGaa                                                                                                                                  <  2:10340531/140‑1 (MQ=255)

     ccGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCCTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGaa                                                                                                                                  <  1:7180486/140‑1 (MQ=255)

        ctgctTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTATCGGCACTGAACGc                                                                                                                               >  2:1509462/1‑140 (MQ=255)

         tgctTGCGCAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGcc                                                                                                                              >  2:3817189/1‑140 (MQ=255)

            ttGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCg                                                                                                                           <  2:8930081/140‑1 (MQ=255)

             tGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCgg                                                                                                                          <  1:10762137/140‑1 (MQ=255)

             tGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCgg                                                                                                                          <  2:6837391/140‑1 (MQ=255)

             tGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCgg                                                                                                                          <  1:9083375/140‑1 (MQ=255)

               cGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGgcg                                                                                                                        <  1:6631103/140‑1 (MQ=255)

               cGAAGAAAAAGTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGgcg                                                                                                                        <  1:7395181/140‑1 (MQ=255)

                 aagaaAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCACCCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCt                                                                                                                      <  1:4189290/140‑1 (MQ=255)

                   gaaAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGt                                                                                                                    <  2:10934355/140‑1 (MQ=255)

                         ttCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCAATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCt                                                                                                              <  1:2792867/140‑1 (MQ=255)

                            agcagTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGc                                                                                                           >  2:10442293/1‑140 (MQ=255)

                             gcagTCAGACTGTAGGCCACACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCa                                                                                                          <  2:7387206/140‑1 (MQ=255)

                             gcagTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCa                                                                                                          <  2:8787251/140‑1 (MQ=255)

                             gcagTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCa                                                                                                          <  1:8922910/140‑1 (MQ=255)

                             gcagTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCa                                                                                                          <  1:6404092/140‑1 (MQ=255)

                             gcagTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCa                                                                                                          <  2:780969/140‑1 (MQ=255)

                             gcagTCAGAATGTAGGCCTCACCATTACGGAAACGCCGTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCa                                                                                                          <  2:1653168/140‑1 (MQ=255)

                                       tggaGGCCACACCATTCCGGCAACGCCTGCCTCCCGCGAGGCCAGTGACCCCATCGACATGGATCAAACCCGCCCGCAGGCAATATGGGGCTTTACCGGCCCGGACCGCCCCGGCGCTGTTTACCTGGCTGCGGctacag                                                                                                <  2:4320282/137‑5 (MQ=255)

                                        gTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAACCCATCGCCATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGc                                                                                               <  1:5613193/140‑1 (MQ=255)

                                        gTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGc                                                                                               <  2:2919628/140‑1 (MQ=255)

                                           ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACCTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCGGTTTACCTGGCAGCGGCTCTGGCAGc                                                                                            >  2:9020989/1‑140 (MQ=255)

                                           ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAAACCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCGGTTTACCTGGCAGCGGCTCTGGCAGc                                                                                            >  2:6348329/1‑140 (MQ=255)

                                              cTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTca                                                                                         <  1:6804129/140‑1 (MQ=255)

                                               tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcac                                                                                        <  1:2339596/140‑1 (MQ=255)

                                               tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcac                                                                                        <  1:6941013/140‑1 (MQ=255)

                                                cACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcaca                                                                                       <  1:6484765/140‑1 (MQ=255)

                                                 cccATTCCGGTAACGCCTTTCTGGTGCTATGGCAGGGAAACCCTCGACATGGTTCCAACCCGCCCGAAGGCCCTGTGGTGCTTTAACGGCACTGAACGCCCCGGCGCGGGTTACCTGGCAGCGGCTCTGGCAGCTCACAg                                                                                      >  2:3021019/2‑140 (MQ=255)

                                                 aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAg                                                                                      >  2:8693895/1‑140 (MQ=255)

                                                   cATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGcc                                                                                    >  1:5901955/1‑140 (MQ=255)

                                                      tACGGTACCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGACGGCCAGTTGGGGCGGTACCGGCACTGACCGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCGCGCAGcctgt                                                                                 >  2:9479368/1‑137 (MQ=255)

                                                      tACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGa                                                                                 >  1:11054743/1‑140 (MQ=255)

                                                      tACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGa                                                                                 >  2:3966456/1‑140 (MQ=255)

                                                       aCGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGaa                                                                                <  1:1654568/140‑1 (MQ=255)

                                                        cGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGaaa                                                                               >  2:3618514/1‑140 (MQ=255)

                                                          gTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAgg                                                                             <  2:3259500/140‑1 (MQ=255)

                                                          gTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAgg                                                                             >  1:10478298/1‑140 (MQ=255)

                                                          gTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAgg                                                                             <  2:6038071/140‑1 (MQ=255)

                                                           tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGc                                                                            >  1:6998232/1‑140 (MQ=255)

                                                           tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGc                                                                            >  1:8639418/1‑140 (MQ=255)

                                                           acaCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTGAACGGCACTGAACGCCCCGGCGCGGGTTACCTGGCAGCGGCTCTGGCAGCTCACAGCGAGAACGGc                                                                            >  2:8140844/3‑140 (MQ=255)

                                                             aCGCCTTGCTGGTGCTATGGNAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCGGTTTACCTGNCAGCGGCNCTGGCAGCTCACAGCCAGAAAGGCag                                                                          >  1:6046367/1‑139 (MQ=255)

                                                             aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCAt                                                                          >  2:10730730/1‑140 (MQ=255)

                                                                ccTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATccc                                                                       >  2:826808/1‑140 (MQ=255)

                                                                ccTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATccc                                                                       >  1:4820229/1‑140 (MQ=255)

                                                                 cTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCa                                                                      <  2:8538508/140‑1 (MQ=255)

                                                                   tGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGc                                                                    >  1:131468/1‑140 (MQ=255)

                                                                   tGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGc                                                                    >  2:2119907/1‑140 (MQ=255)

                                                                      tGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGCCGGCCAGTGGGGGCTTTACCGGCCCTGACCGCCCCGGCGCTGTGTCCCTGGCAGCGGCTCTGGCAGCTCACCGCCAGACAGGCCTCCCAGCCtt                                                                 <  2:1972565/140‑1 (MQ=255)

                                                                       ggTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATtc                                                                <  1:770242/140‑1 (MQ=255)

                                                                       ggTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATtc                                                                <  1:7581671/140‑1 (MQ=255)

                                                                         tGCTATGGCAGTGAAACCATCGACCTGGATCCAACCCGCCCGATGGCCATTTGGGGCTTTTACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCTCTGCGAGTTAGGCATCCCTGCATtctg                                                              >  2:5537609/1‑139 (MQ=255)

                                                                             aTGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCAtt                                                          <  1:1943461/140‑1 (MQ=255)

                                                                              tGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCAttt                                                         >  2:4551885/1‑140 (MQ=255)

                                                                               ggCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTa                                                        <  1:269605/140‑1 (MQ=255)

                                                                                   gTGAAACCATCGACATGGATCCCACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTGTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGCAAGGCCTCCCAGCATTCGCCATTTACggg                                                    >  2:7986506/1‑139 (MQ=255)

                                                                                   gTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCCTTTACGGt                                                    >  2:9687920/1‑140 (MQ=255)

                                                                                   gTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGt                                                    >  1:979128/1‑140 (MQ=255)

                                                                                   gTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGt                                                    <  1:10048491/140‑1 (MQ=255)

                                                                                   gTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGt                                                    >  1:9251006/1‑140 (MQ=255)

                                                                                   gTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGt                                                    >  1:4477207/1‑140 (MQ=255)

                                                                                     gAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCa                                                  <  1:4276939/140‑1 (MQ=255)

                                                                                     gAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCa                                                  <  2:7696838/140‑1 (MQ=255)

                                                                                      aaaCCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCAt                                                 <  2:2354251/140‑1 (MQ=255)

                                                                                       aaCCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATg                                                <  1:2478127/140‑1 (MQ=255)

                                                                                           aTCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGt                                            >  2:1275456/1‑140 (MQ=255)

                                                                                            tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcac                                                                                        <  2:5789514/95‑1 (MQ=255)

                                                                                            tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGtt                                           >  1:3234199/1‑140 (MQ=255)

                                                                                                  tGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGAt                                     <  2:11138828/140‑1 (MQ=255)

                                                                                                    gATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGc                                   <  1:10248362/140‑1 (MQ=255)

                                                                                                     aTCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGcc                                  >  1:5381718/1‑140 (MQ=255)

                                                                                                          aCCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGACAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGa                             >  2:10094819/1‑140 (MQ=255)

                                                                                                          aCCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGa                             <  1:3791563/140‑1 (MQ=255)

                                                                                                          aCCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGa                             >  1:624881/1‑140 (MQ=255)

                                                                                                           cccgcccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGac                            >  1:3064468/1‑140 (MQ=255)

                                                                                                               cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAt                        >  1:9596505/1‑140 (MQ=255)

                                                                                                               cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAt                        >  1:11224712/1‑140 (MQ=255)

                                                                                                               cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAt                        >  1:3952946/1‑140 (MQ=255)

                                                                                                               cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAt                        >  2:4809179/1‑140 (MQ=255)

                                                                                                               cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATGTACGGTCATGACGTTCAGGATGCCGATGACACAt                        >  1:9184434/1‑140 (MQ=255)

                                                                                                               cccgAAGGCCATTTGGGGCGTTAACGGCACTGAACGCCCCGGCGCGGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCTTCCCAGCAGTCTCGATGTACGGTCCTGACGTTCAGGCTGCCGCTGACACAt                        >  2:9195124/1‑140 (MQ=255)

                                                                                                                ccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATc                       <  2:6653383/140‑1 (MQ=255)

                                                                                                                        cATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTg               >  1:796014/1‑140 (MQ=255)

                                                                                                                              gggCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATg         <  1:806749/140‑1 (MQ=255)

                                                                                                                               ggCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCTTTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGt        <  2:6169079/140‑1 (MQ=255)

                                                                                                                               ggCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGt        <  1:8367200/140‑1 (MQ=255)

                                                                                                                               ggCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGt        <  2:3324552/140‑1 (MQ=255)

                                                                                                                                gCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGtt       >  1:11374883/1‑140 (MQ=255)

                                                                                                                                   ttAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTgaa    >  2:5106371/1‑140 (MQ=255)

                                                                                                                                    tAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTgaag   >  2:7245684/1‑140 (MQ=255)

                                                                                                                                    tAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTgaag   >  1:1237268/1‑140 (MQ=255)

                                                                                                                                     aaCGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTgaaga  >  1:9971517/1‑140 (MQ=255)

TGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGA  >  NZ_CP025268/2938086‑2938358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`