BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,131,886	A→G	I200T (ATC→ACC)	yghO ←	protein YghO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,131,886	0	A	G	99.7%	2139.6 / NA	614	I200T (ATC→ACC)	yghO	protein YghO
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (245/365); minor base T (1/1); total (246/368)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 614 total aligned reads displayed.

AGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGATCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATACCACGG  >  NZ_CP025268/3131751‑3132022

aGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCAc                                                                                                                                       >  1:3967964/1‑140 (MQ=255)

    gATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGACTGGTCACCttt                                                                                                                                   >  2:9151994/1‑140 (MQ=255)

    gATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCttt                                                                                                                                   >  1:272538/1‑140 (MQ=255)

     aTCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCtttt                                                                                                                                  >  2:9691381/1‑140 (MQ=255)

     aTCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCtttt                                                                                                                                  >  1:3010665/1‑140 (MQ=255)

     aTCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCtttt                                                                                                                                  >  2:6363658/1‑140 (MQ=255)

     aTCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCtttt                                                                                                                                  >  2:7936681/1‑140 (MQ=255)

       cGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACTAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTg                                                                                                                                <  2:8560897/140‑1 (MQ=255)

       cGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTg                                                                                                                                <  1:3727045/140‑1 (MQ=255)

       cGCCCATGGTCGCGAAGTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTg                                                                                                                                <  2:5858578/140‑1 (MQ=255)

       cGCACATGGTCGCGAATTCATGTTCGTTAAACTGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTg                                                                                                                                <  2:1326414/140‑1 (MQ=255)

         cccATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTgcg                                                                                                                              <  1:5349791/140‑1 (MQ=255)

           cATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCa                                                                                                                            <  1:7202773/140‑1 (MQ=255)

           cATGGTCGCGAATTCATGTTCGCTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCa                                                                                                                            <  2:3106534/140‑1 (MQ=255)

             tGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCAcc                                                                                                                          <  1:1337507/140‑1 (MQ=255)

             tGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCAcc                                                                                                                          <  2:1014003/140‑1 (MQ=255)

                   cgAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATcc                                                                                                                    >  2:3213505/1‑140 (MQ=255)

                   cgAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATcc                                                                                                                    >  2:6160198/1‑140 (MQ=255)

                    gAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCa                                                                                                                   >  1:6138730/1‑140 (MQ=255)

                         cATGTTCGGTACACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑t                                                                                                             <  1:7869446/140‑1 (MQ=255)

                            gTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑tttt                                                                                                          >  1:8233064/1‑140 (MQ=255)

                                ggTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAAAGGTCACCTTTTTGCGCACCTGATCCATCAG‑tttttttc                                                                                                      >  2:10533541/1‑139 (MQ=255)

                                  tAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAgc                                                                                                    >  2:1762468/1‑140 (MQ=255)

                                  tAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAgc                                                                                                    >  2:2701823/1‑140 (MQ=255)

                                    aaCGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAgcgc                                                                                                  <  2:4297568/140‑1 (MQ=255)

                                    aaCGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAgcgc                                                                                                  <  2:10669535/140‑1 (MQ=255)

                                      cGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCg                                                                                                <  1:4751660/140‑1 (MQ=255)

                                          actcaTCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCTGGATCTGCATCTCTTCGGCAAACCGCTGACGTTTGTTGCAGCTTTTGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTTGCGCCGgaga                                                                                            <  2:7315593/136‑1 (MQ=255)

                                          acaAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGgaga                                                                                            <  1:426829/140‑1 (MQ=255)

                                           caAATACACAGTTGTGCTGCCATCCAGAGTTGAAAATCTCTCGCATTATATGCATCTCTTCGTCAAACCTCTGACGATTGATGCAGCGTATGTTCACCTTTTTGCGCACCTGATCCATCAT‑TTTTTTTAGCGCCTgagag                                                                                           <  2:11263369/140‑1 (MQ=255)

                                             aaTCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGaa                                                                                         <  1:6717738/140‑1 (MQ=255)

                                                 cccAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTg                                                                                     >  1:9222434/1‑140 (MQ=255)

                                                   cAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTgag                                                                                   >  1:1200734/1‑140 (MQ=255)

                                                    aGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTGAGCGCCGGAGAGAAAGTgaga                                                                                  <  2:4867956/140‑1 (MQ=255)

                                                      ttGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATc                                                                                >  1:10758175/1‑140 (MQ=255)

                                                       tgtgCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCg                                                                               <  1:1789409/140‑1 (MQ=255)

                                                        gtgCTGCCAGCCAGATGTGAAAATCTAACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTCGCGCCGGAGAGACCGTGAGATCgg                                                                              >  2:2819065/1‑140 (MQ=255)

                                                         tgctgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGt                                                                             >  1:1973786/1‑140 (MQ=255)

                                                         tgctgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGt                                                                             <  1:7984327/140‑1 (MQ=255)

                                                           ctgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTc                                                                           >  2:4463195/1‑140 (MQ=255)

                                                           ctgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTc                                                                           <  1:10650051/140‑1 (MQ=255)

                                                            tgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCg                                                                          <  2:9361745/140‑1 (MQ=255)

                                                              ccagccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCt                                                                        >  1:5809344/1‑140 (MQ=255)

                                                              ccagccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCt                                                                        <  2:1097686/140‑1 (MQ=255)

                                                                agccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGc                                                                      >  1:11377931/1‑140 (MQ=255)

                                                                 gccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCa                                                                     <  1:10360232/140‑1 (MQ=255)

                                                                   cagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATc                                                                   >  2:5289638/1‑140 (MQ=255)

                                                                   cagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACATTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGCAAGTGAGATCGGTTCGCTGCATc                                                                   >  2:7529419/1‑140 (MQ=255)

                                                                      agTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATccac                                                                >  2:7094952/1‑140 (MQ=255)

                                                                      agTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATccac                                                                >  1:11118395/1‑140 (MQ=255)

                                                                        ttGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATccacca                                                              <  2:9600652/140‑1 (MQ=255)

                                                                        ttGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATccacca                                                              <  1:7002187/140‑1 (MQ=255)

                                                                         tGAAAATCTCACGCAGTATCTGCATCTCTTCCGCACACAGCCGACGATTGATGCAGCGCATGGTCACCCTTTTGCGCACCAGATCCACCAC‑TAATTTTATCGCCGGAGAGAACGAGAGATCGGTTCGCTGCATccacccc                                                             <  2:1839056/140‑3 (MQ=255)

                                                                         tGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCcaccac                                                             <  1:6487521/140‑1 (MQ=255)

                                                                               tctcACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGt                                                       <  2:3998559/140‑1 (MQ=255)

                                                                               tctcACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGt                                                       <  1:10033221/140‑1 (MQ=255)

                                                                               tctcACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGt                                                       <  1:2566801/140‑1 (MQ=255)

                                                                                ctcACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTa                                                      >  2:346135/1‑140 (MQ=255)

                                                                                     gCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCa                                                 <  2:2212997/140‑1 (MQ=255)

                                                                                      cAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCaa                                                <  2:9907796/140‑1 (MQ=255)

                                                                                      cAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCaa                                                <  2:3758323/140‑1 (MQ=255)

                                                                                      cAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCaa                                                <  1:5984149/140‑1 (MQ=255)

                                                                                       aGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTTAGCGCCGTAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCaa                                                <  2:5551373/140‑1 (MQ=255)

                                                                                       aGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAAt                                               <  1:9616885/140‑1 (MQ=255)

                                                                                         gATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGc                                             >  1:859912/1‑140 (MQ=255)

                                                                                           tCTGCATCACGTCGGCCAACCGCAGACGATCGATGCAGCGAATGGTCACCTTTTGGCGCACCTGATCCAGCAG‑TTTTTTTAGCGCCGGAGAGAACGTGAGATCGGTTCGCTGCATCCACCACGCCACTAAATCAATGCCt                                           <  2:11161012/140‑1 (MQ=255)

                                                                                            cTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCtt                                          <  2:10778054/140‑1 (MQ=255)

                                                                                            cTGCATCACTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCtt                                          <  1:8765979/140‑1 (MQ=255)

                                                                                               cATCTCTTCGGCAACCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTtgt                                       <  2:5815757/140‑1 (MQ=255)

                                                                                               cATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCTTCCACCACGCCAGTAAATCAATGCCTTtgt                                       <  2:4999253/140‑1 (MQ=255)

                                                                                                   tctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATa                                   >  2:8901282/1‑140 (MQ=255)

                                                                                                   tctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATa                                   >  1:5126440/1‑140 (MQ=255)

                                                                                                    ctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATaa                                  >  1:1047636/1‑140 (MQ=255)

                                                                                                     ttCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAc                                 >  1:9522422/1‑140 (MQ=255)

                                                                                                     ttCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAc                                 >  1:6544049/1‑140 (MQ=255)

                                                                                                     ttCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCAGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAc                                 >  2:2399944/1‑140 (MQ=255)

                                                                                                      tCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAcc                                <  1:3522963/140‑1 (MQ=255)

                                                                                                      tCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAcc                                <  2:3686870/140‑1 (MQ=255)

                                                                                                      tCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAcc                                <  1:9743805/140‑1 (MQ=255)

                                                                                                      tCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAcc                                <  1:6370593/140‑1 (MQ=255)

                                                                                                        ggCAATCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCa                              <  1:8432291/140‑1 (MQ=255)

                                                                                                        ggCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCa                              <  1:7398003/140‑1 (MQ=255)

                                                                                                         gCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAg                             <  1:5533786/140‑1 (MQ=255)

                                                                                                         gCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAg                             <  2:6642670/140‑1 (MQ=255)

                                                                                                             aCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGACAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAgttgt                         >  2:5070986/1‑140 (MQ=255)

                                                                                                             aCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAACGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAgttgt                         >  2:5162491/1‑140 (MQ=255)

                                                                                                             aCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAgttgt                         >  1:9041257/1‑140 (MQ=255)

                                                                                                             aCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAgttgt                         >  1:450034/1‑140 (MQ=255)

                                                                                                               cGCTGACGATTGATGCAGCGACTGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTc                       <  2:6880393/140‑1 (MQ=255)

                                                                                                               cGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTc                       <  1:10922399/140‑1 (MQ=255)

                                                                                                                  tGACGATTGATGCAGCGAATGGTCACCTTTTGGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAat                    <  2:8014835/140‑1 (MQ=255)

                                                                                                                      gATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATgc                >  2:6777869/1‑140 (MQ=255)

                                                                                                                       aTTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATgcg               <  2:7763075/140‑1 (MQ=255)

                                                                                                                       aTTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTGAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATgcg               <  1:7633071/140‑1 (MQ=255)

                                                                                                                            tGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTACATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCa          <  2:8304986/140‑1 (MQ=255)

                                                                                                                                gCGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGTTTCGCTGCATACACCACGCCAGTAAATCAATGCGTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATAcc      >  2:10395914/1‑140 (MQ=255)

                                                                                                                                 cGAATGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATACCa     <  1:10566266/140‑1 (MQ=255)

                                                                                                                                   aaTGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGTGGCATACCACg   >  1:3305332/1‑140 (MQ=255)

                                                                                                                                   aaTGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATACCACg   <  1:2364301/140‑1 (MQ=255)

                                                                                                                                    aTGGTCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATACCACgg  >  1:149884/1‑140 (MQ=255)

AGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGATCACCTTTTTGCGCACCTGATCCATCAG‑TTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATACCACGG  >  NZ_CP025268/3131751‑3132022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`