Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,232,860 T→C N170N (AAT→AAC)  fadH → NADPH‑dependent 2,4‑dienoyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,232,8600TC99.6% 2606.7 / NA 749N170N (AAT→AAC) fadHNADPH‑dependent 2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (346/399);  minor base A (2/1);  total (348/401)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 749 total aligned reads displayed.

AACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGAC  >  NZ_CP025268/3232723‑3232996
                                                                                                                                         |                                                                                                                                        
aaCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGa                                                                                                                                        <  2:8678247/140‑1 (MQ=255)
  ccGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAAt                                                                                                                                      <  1:7267769/140‑1 (MQ=255)
     tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                                                                   >  1:5759495/1‑140 (MQ=255)
      ttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCt                                                                                                                                  >  1:3029344/1‑140 (MQ=255)
       tCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTg                                                                                                                                 >  2:3724786/1‑140 (MQ=255)
            ccccATTAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGTCGCGGGAGGCAGGATACGTCGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCt                                                                                                                            <  1:6145913/140‑1 (MQ=255)
                aTGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATGTCTGACGCTgcgc                                                                                                                        >  1:10611208/1‑140 (MQ=255)
                 tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                                                                                                       <  1:2457372/140‑1 (MQ=255)
                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                     <  1:2208436/140‑1 (MQ=255)
                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                     <  1:7777810/140‑1 (MQ=255)
                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                     <  1:3969203/140‑1 (MQ=255)
                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                     <  2:1249979/140‑1 (MQ=255)
                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                     <  2:8905072/140‑1 (MQ=255)
                    gTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCa                                                                                                                    >  1:10326115/1‑140 (MQ=255)
                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                  <  2:1743334/140‑1 (MQ=255)
                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                  <  1:7911486/140‑1 (MQ=255)
                       aaGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTCTTTGATCAACGAATTTCTGACGCTGCGCACCAATc                                                                                                                 >  1:8041884/1‑140 (MQ=255)
                       aaGCCATGAAGAGATCCTGCAACTGAGCGACAATGTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGCTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATc                                                                                                                 <  2:7210347/140‑1 (MQ=255)
                        agacATGAAGAGATACTGCAACTGATCGACAATTTCGCCCGCTGCGCTCAACTGGCGCGGGAGGCAGGATACGACGGTGTATAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                                <  2:4485575/137‑1 (MQ=255)
                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                                <  1:5623173/140‑1 (MQ=255)
                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                                <  1:4904051/140‑1 (MQ=255)
                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                                <  2:10739460/140‑1 (MQ=255)
                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                                <  1:4715972/140‑1 (MQ=255)
                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGGTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                                <  1:8586576/140‑1 (MQ=255)
                         gCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGTGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAg                                                                                                               >  2:3599076/1‑140 (MQ=255)
                           cATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCg                                                                                                             <  1:9232720/140‑1 (MQ=255)
                            aTGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGt                                                                                                            >  2:1112/1‑140 (MQ=255)
                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           >  1:2685278/1‑140 (MQ=255)
                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           >  1:9540260/1‑140 (MQ=255)
                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           >  2:3980904/1‑140 (MQ=255)
                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTACCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           >  2:1819018/1‑140 (MQ=255)
                             tGAAGAGATCCTGCAACTGATCGACAAAATCGCCCGCAGCGCGCAACTGGCGCGTGAGGCAGGATACGACGGTGAACAGGTGCAGGGTACCGAACGGTATATGAACAACCAAAATCAGACGCTGCGCACCAATCAGCGTa                                                                                                           >  2:8555756/1‑140 (MQ=255)
                               aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGt                                                                                                         >  1:8695533/1‑140 (MQ=255)
                               aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGt                                                                                                         >  2:2319825/1‑140 (MQ=255)
                               aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGt                                                                                                         <  1:4383040/140‑1 (MQ=255)
                                  agaTCGTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAc                                                                                                      <  2:9107864/140‑1 (MQ=255)
                                  agaTCCTGCAACTGATCGACATTTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAc                                                                                                      <  1:5463564/140‑1 (MQ=255)
                                    ggccTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                    >  2:3333769/3‑140 (MQ=255)
                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                    >  2:6463331/1‑140 (MQ=255)
                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                    >  1:1812835/1‑140 (MQ=255)
                                     tCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAg                                                                                                   <  1:7091723/140‑1 (MQ=255)
                                            aCTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTATAGGTGATGGGTTCCGAAGGGTATTTGATCAACGCATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcg                                                                                            >  2:7057862/1‑140 (MQ=255)
                                              tGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcggc                                                                                          >  1:10173317/1‑140 (MQ=255)
                                               caTCGACTACTGCGACCGCTCCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTAATTGATCACCGAAGTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGgcggcg                                                                                         <  2:5485052/139‑1 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGTGGGGTTGCGAAGGGTATTTGATCCTCGACGTGCTGACGCGGCGCGCCAGTCAGCGGAGGGAGCGGTGGGGGGGCGa                                                                                        >  2:6152994/1‑140 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGACTTTCTGACGCTGCGCACCAATCAGCGTAGTGACCCGTGGGGCGGCGa                                                                                        >  2:762707/1‑140 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                        >  2:7909654/1‑140 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                        >  1:75538/1‑140 (MQ=255)
                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATGTGATCCACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                        >  1:6315039/1‑140 (MQ=255)
                                                          tCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAAc                                                                              <  1:3804202/140‑1 (MQ=255)
                                                           cGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAAcc                                                                             >  1:8928431/1‑140 (MQ=255)
                                                                  tGCGCGCAACTGGCGCGGGAGGCCGGATACGAGGGTGTAGAGGTGATGGGTTCCGACGGGGATTTGATCAACGAATTTGTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGAGTAGCGCACGCGGATGCg                                                                      >  2:8347112/1‑140 (MQ=255)
                                                                    cgcgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAATGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAt                                                                    <  1:11105903/140‑1 (MQ=255)
                                                                      cgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGTGGAGGCGTTTACCGCAACCGGATGCGAttt                                                                  <  2:4161754/140‑1 (MQ=255)
                                                                      cgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAttt                                                                  <  1:6545007/140‑1 (MQ=255)
                                                                       gcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTg                                                                 <  2:4313/140‑1 (MQ=255)
                                                                        caccTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGACTTTCTGACGCTGCGCACCACTCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                                <  2:9724568/137‑1 (MQ=255)
                                                                        cAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                                <  2:2175442/140‑1 (MQ=255)
                                                                        cAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                                <  1:9734862/140‑1 (MQ=255)
                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTCGAGGTGAGGGGTTCCGAAGGGTAATTGATCAACGACAGTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATGACCGCACCCGGATGCGAAGTGCCg                                                              <  2:681323/140‑1 (MQ=255)
                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                              <  2:28594/140‑1 (MQ=255)
                                                                            tGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTa                                                            >  2:670278/1‑140 (MQ=255)
                                                                                   ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagtag                                                     <  2:4825000/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  1:8166995/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  2:10039032/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  1:11458236/140‑1 (MQ=255)
                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                               <  2:3260691/140‑1 (MQ=255)
                                                                                           gATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCACTCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGtgcgtgcg                                             <  2:8121347/140‑1 (MQ=255)
                                                                                           gATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGtgcgtgcg                                             <  1:1614799/140‑1 (MQ=255)
                                                                                               cgacgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCgtgcggtgc                                         >  2:10199751/1‑140 (MQ=255)
                                                                                                gacgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCgtgcggtgcg                                        >  2:1552453/1‑140 (MQ=255)
                                                                                                  cgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGcgcg                                      >  1:4104661/1‑140 (MQ=255)
                                                                                                    gtgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCCACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGaa                                    <  2:2004879/140‑1 (MQ=255)
                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           <  1:10886071/68‑1 (MQ=255)
                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                           >  2:10886070/1‑68 (MQ=255)
                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                   >  2:3887592/1‑140 (MQ=255)
                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                   >  1:10012464/1‑140 (MQ=255)
                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                   >  1:3348918/1‑140 (MQ=255)
                                                                                                     agtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCAGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                   >  2:11421881/2‑140 (MQ=255)
                                                                                                       tAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                              >  2:5405906/1‑111 (MQ=255)
                                                                                                            gTGATTGGTTCCCAACGCAATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTTGTGACCGGTGGGGCGGCGAGTACCGCATCCGGCTGCGGGGTGGCGTCGAAGGGGGGCGTGCGGTGCGCGGCGGCGTCgg                            >  2:7450943/1‑140 (MQ=255)
                                                                                                               ctGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGGAGTGACCCGTGGGGCGGCGATTACCGCAACCGGCTGCGATTTGCCGTAGAAGTCGTGCGTGCGGTGCGCGAACGCGTCGGcca                         >  2:11229170/2‑138 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTTGTGACCAGTGGGGCGGCGATTACAGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGcta                         >  2:9639709/1‑138 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  2:10606545/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  2:1424946/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         >  2:10328972/1‑140 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGAGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                         <  2:9286925/140‑1 (MQ=255)
                                                                                                               aTGGGTTCCGAAGGGTATTTGAACAAAGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATGTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCac                         >  2:5737502/1‑139 (MQ=255)
                                                                                                                   gTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGAc                     <  2:9425878/140‑1 (MQ=255)
                                                                                                                   gTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGAc                     <  1:4586324/140‑1 (MQ=255)
                                                                                                                     tCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACtt                   <  2:5948240/140‑1 (MQ=255)
                                                                                                                     tCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACtt                   <  1:4244698/140‑1 (MQ=255)
                                                                                                                       cGAAGGGTATTTGATCAACGAATGTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGAGCGGTGCGCGAACGCGTCGGCAACGACTTCa                 <  2:10479982/140‑1 (MQ=255)
                                                                                                                           gggTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCattat             <  1:6825672/140‑1 (MQ=255)
                                                                                                                              tATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATtattat          >  1:835059/1‑140 (MQ=255)
                                                                                                                              tATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATtattat          >  2:5249169/1‑140 (MQ=255)
                                                                                                                               aTTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACGTCATTATTATc         >  2:5069520/1‑140 (MQ=255)
                                                                                                                                tttGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGACCGCGTCGGCAACGACGTCATTATTATCt        >  2:8258211/1‑140 (MQ=255)
                                                                                                                                   gATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTAcc     >  2:2456865/1‑140 (MQ=255)
                                                                                                                                      cAACGAATTTCTGACGCTGCGCACCAATCAGCGACGTGACCAGTGGGGCGGCGTTTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGTACGCGGCGGCAACGCCTTGGTGAGTATCTACCGag  >  2:2671481/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGAC  >  NZ_CP025268/3232723‑3232996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑