BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,298,720	C→T	G35E (GGA→GAA)	yraQ ←	permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,298,720	0	C	T	99.5%	2993.8 / NA	771	G35E (GGA→GAA)	yraQ	permease
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (352/413); minor base A (0/4); total (353/418)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 775 total aligned reads displayed.

CCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAG  >  NZ_CP025268/3298583‑3298856

ccgcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTtcc                                                                                                                                        >  1:4815453/1‑140 (MQ=255)

  gcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCa                                                                                                                                      <  1:5087613/140‑1 (MQ=255)

  gcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCa                                                                                                                                      <  2:296944/140‑1 (MQ=255)

  gcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCa                                                                                                                                      <  2:1523923/140‑1 (MQ=255)

    tttCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAtt                                                                                                                                    <  2:5880545/140‑1 (MQ=255)

      tCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTc                                                                                                                                  <  2:9355812/140‑1 (MQ=255)

        cATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCAGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTcac                                                                                                                                <  1:3019679/140‑1 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATTGGTTAGCATCCGCCTGCGCAAGTATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                          <  2:8738682/140‑1 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                          >  1:6441811/1‑140 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                          <  1:6290346/140‑1 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                          >  2:11299069/1‑140 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                          <  1:1341242/140‑1 (MQ=255)

               cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                                                                                                                         >  2:2718521/1‑140 (MQ=255)

               cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTCCGCATGGCTCGTTGTACCGATACGGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCTCATACCag                                                                                                                         >  2:7109574/1‑140 (MQ=255)

                gAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCaga                                                                                                                        >  2:803613/1‑140 (MQ=255)

                  ggAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAgaga                                                                                                                      <  2:1303548/140‑1 (MQ=255)

                  ggAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAgaga                                                                                                                      <  2:2082368/140‑1 (MQ=255)

                  ggAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAgaga                                                                                                                      <  1:9542666/140‑1 (MQ=255)

                        aGATCATCGCGTACTCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCa                                                                                                                <  1:7145364/140‑1 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCTCATACCAGAGACCGGCAATGACa                                                                                                          >  1:561296/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                          >  1:1860264/1‑140 (MQ=255)

                                    aaTCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCACTGACAACgaga                                                                                                    >  1:617606/1‑140 (MQ=255)

                                         aaCGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGagaaagaa                                                                                               <  1:2875399/140‑1 (MQ=255)

                                           cgccgcCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGaaaa                                                                                             >  2:3260093/1‑140 (MQ=255)

                                           cgccgcCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGaaaa                                                                                             >  1:2254199/1‑140 (MQ=255)

                                            gccgccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAg                                                                                            >  2:4564481/1‑140 (MQ=255)

                                               gccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                                         <  2:5575393/140‑1 (MQ=255)

                                               gccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                                         <  1:2456882/140‑1 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACACCGAGAAAGAAAAGcgcg                                                                                        >  2:6211254/1‑140 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGCAAGAAAAGcgcg                                                                                        >  1:10662754/1‑140 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                        >  1:9024885/1‑140 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                        >  2:6971003/1‑140 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                        >  2:8888584/1‑140 (MQ=255)

                                                     cATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCTATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGttt                                                                                   <  1:397308/140‑1 (MQ=255)

                                                         ggTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccac                                                                               <  1:3883562/140‑1 (MQ=255)

                                                                    gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                    >  1:6775045/1‑140 (MQ=255)

                                                                    gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                    >  2:6627867/1‑140 (MQ=255)

                                                                    gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                    >  1:1122301/1‑140 (MQ=255)

                                                                         cgcAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTg                                                               <  1:2005605/140‑1 (MQ=255)

                                                                          gccaGGATAGATTTACCGATACGGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGAAAGGTTCACATCTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTgc                                                              <  2:8673458/137‑1 (MQ=255)

                                                                           cAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTgcc                                                             <  1:9911844/140‑1 (MQ=255)

                                                                           cAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTgcc                                                             <  1:9384459/140‑1 (MQ=255)

                                                                              ggATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAACAGAGACCGGCAATGACAACGAGAAAGACAAGCGCGGGTTTCCACCACTGCAGGGGCGTTgccgcc                                                          >  1:8435750/1‑140 (MQ=255)

                                                                               gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                         <  1:921390/140‑1 (MQ=255)

                                                                               gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                         <  1:4207189/140‑1 (MQ=255)

                                                                                  aGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTgag                                                      <  1:11042206/140‑1 (MQ=255)

                                                                                   gATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAACGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTgaga                                                     >  2:9069178/1‑140 (MQ=255)

                                                                                        aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAg                                                >  2:1752484/1‑140 (MQ=255)

                                                                                        aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAg                                                >  2:5461675/1‑140 (MQ=255)

                                                                                        aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAg                                                >  2:2538243/1‑140 (MQ=255)

                                                                                        aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAg                                                >  1:1605587/1‑140 (MQ=255)

                                                                                         ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                               >  2:10593067/1‑140 (MQ=255)

                                                                                         ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                               <  2:2412066/140‑1 (MQ=255)

                                                                                         ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                               <  1:2718521/140‑1 (MQ=255)

                                                                                          cGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCAGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGAc                                              >  2:5019596/1‑140 (MQ=255)

                                                                                             tACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                                           <  1:5874137/140‑1 (MQ=255)

                                                                                             tACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACAGAACAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCTGGTTTCCACCTCTGAATGGGCGTTGCAGCCTGAGATGAAGACAGa                                           <  2:468804/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCACTGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:7737776/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:3494845/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:3581164/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:5327348/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:8839355/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          >  1:3624957/1‑140 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:8123066/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:8656974/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGGAAACGCTTGGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:10939493/140‑1 (MQ=255)

                                                                                              aCTGTCGGCTTCGGCAGCAGTAAACGCTTTGCCGAAGTAAGGGTCCCAGTTCCCATACCAGAGACCGGCAATGACAACGAGCAAGACAAGCGCGGGTTTCCACCACTGAAGGGGCGTTGCCGCCTGAGATGCAGCCTGAc                                          <  2:11440959/140‑1 (MQ=255)

                                                                                               cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAcc                                         <  2:4901398/140‑1 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          >  1:9658939/1‑136 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      <  1:3177614/140‑1 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:10478188/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  2:3718438/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:7428441/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:821226/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:4407964/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  2:437784/1‑140 (MQ=255)

                                                                                                   gggTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCAATTTCACATACCAGAGACCGGCAATGACAACCATAAAGAAAAGAGCGGGTTTCCACCACAGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTc                                     >  2:2009814/1‑140 (MQ=255)

                                                                                                       ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGCAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAg                                 >  2:5701374/1‑140 (MQ=255)

                                                                                                         cGGCAGCAGTAACCGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  2:9244219/140‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  1:3290066/140‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  1:1514369/140‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  1:7805915/140‑1 (MQ=255)

                                                                                                          ggCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCAt                              <  1:698477/140‑1 (MQ=255)

                                                                                                               cagTAAACGCTTTGCAGTATTAAGGTTAACATTTCACATAACAGAGACCGGCACTGACAACGAGAAAGAAAAGCGCGGGTTTCCACCAATGATTGGGGGTTGCCGCCTGAGATGAAGACTGACCAGTCTTAGCTTTcccc                         <  2:760497/140‑1 (MQ=255)

                                                                                                               cagTAAACGCTGTGCCGTAGTAAGGGTCCCAGTTCACAAACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTcccc                         <  2:7345239/140‑1 (MQ=255)

                                                                                                                       gCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATaa                 <  2:10468628/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCCAGTCACAGACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCTGTCATAGCATTCCCCAGGAATAAGt               <  2:5375364/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:957224/113‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  1:10036751/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCAGTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCAACCACTGAATGGGCGTTGCCGCCTGAGATGAATACTTACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:4024667/140‑1 (MQ=255)

                                                                                                                          ttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGtt              >  1:8009091/1‑140 (MQ=255)

                                                                                                                           tGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa             <  1:10156616/140‑1 (MQ=255)

                                                                                                                           tGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa             <  1:4548879/140‑1 (MQ=255)

                                                                                                                           tGCCGGAGTAAGGTTCCCATTTCACATACCAGAGGACGGCAATTACAACGTGAAAGAATAGCGCGGGGTTCCACCACTGAATGGGCGTTGCCGCCTGATATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa             <  2:10088932/140‑1 (MQ=255)

                                                                                                                            gCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGAAATGAAAACTAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCTCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCATGAATAAGTTAt            <  2:3950286/140‑1 (MQ=255)

                                                                                                                               gtagtaAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGAt         <  1:11427721/140‑1 (MQ=255)

                                                                                                                                    aaGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTg    <  2:8277793/140‑1 (MQ=255)

                                                                                                                                      ggTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAg  <  2:6477896/140‑1 (MQ=255)

                                                                                                                                      ggTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAg  <  1:7580641/140‑1 (MQ=255)

                                                                                                                                      ggTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAg  <  2:9769166/140‑1 (MQ=255)

CCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAG  >  NZ_CP025268/3298583‑3298856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`