BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,608,154	G→A	noncoding (48/87 nt)	CXP41_RS23870 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,608,154	0	G	A	96.4%	1454.3 / NA	445	noncoding (48/87 nt)	CXP41_RS23870	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (4/2); major base A (214/209); minor base T (8/8); total (226/219)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608020‑4608290

ccACGTCCGTATGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  1:3910929/140‑1 (MQ=255)

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCTCGTATACCAATTCCGCCACCTTCTCACAGTCATCTTACTTTTTTTGATCTCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTTAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  1:475928/140‑1 (MQ=37)

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     >  2:7563498/1‑140 (MQ=255)

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATAACTTTATTAGATATAGAGTCTTTTAGTGCGA‑GGGGCTGGAGGTGAACCCCCAGGTCCGTCAGTacaat                                                                                                                                     >  1:10837732/1‑138 (MQ=17)

    gTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                                                                 >  1:1792557/1‑140 (MQ=255)

      ccGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                               <  2:9347961/140‑1 (MQ=255)

               cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                                                      <  1:2828573/140‑1 (MQ=255)

                 cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                                                    <  1:6787327/140‑1 (MQ=255)

                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTGTTTTTGATGTCGCCTCGTTTGGGGCGA‑GGGGGGGGACTTGAACCCCGACGTCCGTACGAACACTAACACCTGAAGCTAgcgc                                                                                                                   >  2:2264523/1‑140 (MQ=37)

                   aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCAACTTCGCACAGTCATCTTACTATTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                  <  2:2363074/140‑1 (MQ=255)

                    acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                                                                                                 >  1:8240303/1‑140 (MQ=255)

                    acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                                                                                                 >  1:2980924/1‑140 (MQ=255)

                       ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                                                              >  1:8519025/1‑140 (MQ=255)

                        cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                                             >  2:9709924/1‑140 (MQ=255)

                        cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                                             >  2:5491703/1‑140 (MQ=255)

                        cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                                             >  1:11321804/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                           >  2:8228237/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                           >  1:767078/1‑140 (MQ=255)

                            aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                                                         >  1:4062323/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTCCTTTATTTGATATCGCCTCGGTTGGTGCGA‑GGCGGGGGACTTGAACCCACACGTCCGTAAGAACCCTAACACCTGAAGCTAGCGCGTCTACCAAGTCCg                                                                                                     >  2:7038320/1‑140 (MQ=25)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                     >  1:5490736/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTAACTTTTTTTGATATCGCCTCGATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTCACACCTGAAGCTAGCGCGTCTACCAATTcag                                                                                                     >  2:9058561/1‑138 (MQ=25)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCAAAGACAAATTACTTAATTTGATATCGAATCTATTGGTCCGA‑TGCGTGGGACTTTAACCAAAACGTCAATAATAACACAAACACCTTGAGCTAAATCCTCTACAAATTACg                                                                                                     >  2:314565/1‑140 (MQ=255)

                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    <  1:9100517/140‑1 (MQ=255)

                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    <  2:10151828/140‑1 (MQ=255)

                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    <  1:4853902/140‑1 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    >  1:10034970/1‑140 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                   >  2:1714600/1‑140 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTGGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                   >  2:10645943/1‑140 (MQ=255)

                                     gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                                >  1:69636/1‑140 (MQ=255)

                                      tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGTAGCTAGCGCGTCTACCAATTCCGCCCCCt                                                                                               >  1:6678794/1‑140 (MQ=255)

                                      tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                                               >  1:9709046/1‑140 (MQ=255)

                                         aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                                            >  1:11096756/1‑140 (MQ=255)

                                          ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                                           <  1:4646461/140‑1 (MQ=255)

                                          ccAAATCCGGCATCTACTCACAGTCATCTAACTATTTTGGATATAGACTCTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTCTACCAATTCCGCCACCTTCGc                                                                                           <  2:3630366/140‑1 (MQ=14)

                                          acAATTCCGCCACCTTCTCACAGTCATCTTACATTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                                           <  2:10881565/139‑1 (MQ=255)

                                           ctaTTCCGCCAACTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTCGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:6098815/138‑1 (MQ=21)

                                           cccttCCGCCACCTTCGCACAGTCAACTAACTTTTTATGATATCGCCTCCTTTGGAGCGA‑GGGGGGGGACTAGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:9483744/137‑1 (MQ=18)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:3533533/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:935198/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:2165931/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:1023261/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:557130/140‑1 (MQ=255)

                                           cAACTCCGCCACCTTCGCACCGTCATCTAACTTTATTGGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGcc                                                                                          <  2:6301900/140‑1 (MQ=18)

                                            aaTTCCGCCACCTTAGCACATTCATCTTAATTTTTTTGATATAGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                                                         <  1:6261863/140‑1 (MQ=18)

                                               tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                                      <  2:1421966/140‑1 (MQ=255)

                                                  gCCACCTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGCCCAGTGCg                                                                                   <  2:725797/140‑1 (MQ=255)

                                                  gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                                   <  1:537530/140‑1 (MQ=255)

                                                  gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                                   <  2:2540021/140‑1 (MQ=255)

                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 >  2:2834725/1‑140 (MQ=255)

                                                              caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACNCCAGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCNATATCAAc                                                                       <  1:6135594/140‑1 (MQ=255)

                                                                gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                                                     >  1:7574653/1‑140 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                    >  1:7211024/1‑140 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGCGAGCAATATCAACGTg                                                                    >  1:9998406/1‑140 (MQ=255)

                                                                    tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt                                                                 >  2:5267546/1‑140 (MQ=255)

                                                                         cTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGAGCAATATCAACGTGGTTGTTgg                                                            >  2:2684224/1‑140 (MQ=255)

                                                                         cTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                                                            >  2:3356418/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                          >  1:6383917/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                          >  2:11439054/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                          >  2:5787126/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACAGCCAGCAATATCAAAGTGGTTTTTGCTg                                                          >  1:7303260/1‑140 (MQ=18)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTGGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTGTTGGTg                                                          >  2:6374627/1‑140 (MQ=255)

                                                                             ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                                        <  1:5239743/140‑1 (MQ=255)

                                                                               ttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGCGAg                                                      >  2:3279877/1‑140 (MQ=255)

                                                                                     tCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCACGTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                                                <  2:5045959/140‑1 (MQ=255)

                                                                                     tCGCCTCGTTGGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGGCTACCAAGTCCGCCACCTTCGCCCCGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                                                <  2:6634917/140‑1 (MQ=21)

                                                                                      cGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggg                                               >  1:7792563/1‑140 (MQ=255)

                                                                                        ccTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc                                             >  1:4419693/1‑140 (MQ=255)

                                                                                        ccTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc                                             <  2:4283578/140‑1 (MQ=255)

                                                                                             tttGGTGCGA‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGaa                                        <  1:2353687/140‑1 (MQ=255)

                                                                                             tttGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          >  2:7996673/1‑90 (MQ=14)

                                                                                             tttGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGaa                                        >  1:2523859/1‑140 (MQ=255)

                                                                                              ttGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAc                                       <  2:459371/140‑1 (MQ=255)

                                                                                                  tGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                                                         >  1:8912255/1‑86 (MQ=17)

                                                                                                  tGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACAACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc                                   <  2:10726372/140‑1 (MQ=255)

                                                                                                   gCGA‑GGGGGGGGACATGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                  <  1:5975958/140‑1 (MQ=255)

                                                                                                     gA‑GGGGGGGGACTTGCACCCCCACGTCCGTAAGAACAATACCACCTGAAGCTAGCGCGTCTACCACGTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                <  1:1991845/140‑1 (MQ=25)

                                                                                                     gA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                <  1:3319300/140‑1 (MQ=255)

                                                                                                     gA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                <  1:9158489/140‑1 (MQ=255)

                                                                                                      a‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGt                               >  1:4260756/1‑140 (MQ=255)

                                                                                                       gggggggggACTTGAACCCCCACGTCCGTCTGAACACTAACACCTGAAGCTCGCGCGTCTACCAATTCCGCCAACTTCTCCCAGGGCGAGCAAGATCGACGTGGTTGTGGGGGCGAGGGGGGGGAGTGGAACCCCCACGt                               >  2:11149407/2‑140 (MQ=18)

                                                                                                        ggggtgggACTTGAACCCCCACTTCCGTTAGAACACTAACACCTGAAGCTAGCGCGTCTACCATTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTc                              <  2:8571324/140‑1 (MQ=25)

                                                                                                        ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAGGGGGTGGCCTTGAACCCCCACGTc                              >  2:7651896/1‑140 (MQ=255)

                                                                                                        ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCACTATAAACGTGGTTTTTGGTGCGAGGGGGGGGACGTGAACCACCACGTc                              >  2:10561587/1‑140 (MQ=21)

                                                                                                           gggggACTTGACCCCCCACGACCGTAAGACCACTAACACCTGAAGCTAGCGAGTCAACCTCTACCGCCACCTTCGCCCATTGCGAGCAATATCATCGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCACACGTCCga                           <  2:3862834/140‑2 (MQ=25)

                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCCATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                         >  1:3202568/1‑140 (MQ=255)

                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                         >  2:4159993/1‑140 (MQ=255)

                                                                                                              ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGTGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  1:7977392/140‑1 (MQ=255)

                                                                                                                  ttGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGAAATTGCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCACCACGTCCTTAAGGaca                    >  2:1907272/1‑140 (MQ=21)

                                                                                                                   tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                   >  1:5159778/1‑140 (MQ=255)

                                                                                                                     aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                 <  1:1224997/140‑1 (MQ=255)

                                                                                                                      aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                <  2:4842252/140‑1 (MQ=255)

                                                                                                                      aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTACGCCCAGTGCGAGCAATATCAACGTGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                <  1:11442556/140‑1 (MQ=255)

                                                                                                                       cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAGTTGACCCCCCACGTCCGTTAGGACACTAac               >  1:9268952/1‑140 (MQ=255)

                                                                                                                       cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAac               >  1:10329767/1‑140 (MQ=255)

                                                                                                                           cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAGGGGGTGGACTTGAAACCCCACGTACGTAAGGACACTAACACCt           >  2:6519546/1‑140 (MQ=25)

                                                                                                                           cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt           >  1:3582327/1‑140 (MQ=255)

                                                                                                                                  gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACACCCACGTCCGAAAGGACACTAACACCTGAAGCTa    >  1:150364/1‑140 (MQ=255)

                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg   >  1:3273254/1‑140 (MQ=255)

                                                                                                                                    aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc  >  1:2107933/1‑140 (MQ=255)

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608020‑4608290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`