Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	390,887	G→A	A356V (GCG→GTG)	CXP41_RS02035 ←	IS3‑like element IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	390,887	0	G	A	94.7%	30.6 / ‑6.0	19	A356V (GCG→GTG)	CXP41_RS02035	IS3‑like element IS3 family transposase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (17/1);  total (18/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACGCCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTC  >  NZ_CP025268/390750‑390926                                                                                                                                           |                                        gggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACAcc                                       >  1:10968694/1‑140 (MQ=18) gggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACAcc                                       >  2:5005847/1‑140 (MQ=18) gggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACAGTCGATA‑TAATTAAACAcc                                       >  2:10039831/1‑140 (MQ=16)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCATATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGAGTGTAATCACACTCGATA‑TAATTAAACACCg                                      >  2:10128550/1‑140 (MQ=11)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCg                                      >  1:11214888/1‑140 (MQ=17)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCg                                      >  2:8153026/1‑140 (MQ=17)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCg                                      >  2:7259574/1‑140 (MQ=17)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCg                                      >  2:6005984/1‑140 (MQ=17)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCg                                      >  2:10036349/1‑140 (MQ=17)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCg                                      >  1:9024340/1‑140 (MQ=17)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCg                                      >  1:2946244/1‑140 (MQ=17)  ggggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCg                                      >  1:1209327/1‑140 (MQ=17)  ggggCTGATTGATCCTACCCACGTAATATGGACAAAGGCATAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACAcct                                      >  2:3683396/1‑139 (MQ=12)  ggggCTGATAGAACCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCTGACAGAGGCCGCCACACCAACTGTGCCGCCGCCACCGAATGTAATCACATTCGATA‑TAATTAAACACCg                                      >  2:6309873/1‑140 (MQ=11)   gggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCGt                                     >  2:4301185/1‑140 (MQ=14)    ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCGtt                                    >  1:4974298/1‑140 (MQ=11)    ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCGtt                                    <  1:3920592/140‑1 (MQ=11)    ggCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACACCGtt                                    >  2:8795164/1‑140 (MQ=11)                                      cgcctatgCGAGGTTCTGGGTTTCAAAGTGTTCCGGACTGAGGCCGCCACCCCAACTGTGCCGCCGCCACCGAGGGTAGTCACATTCGTGAGTATTGATACG‑CGTGGCGCGCATTCTTTGCCGGCTGATAAGGTGTtgtc  >  2:7152150/8‑140 (MQ=2)                                                                                                                                           |                                        GGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATA‑TAATTAAACGCCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTC  >  NZ_CP025268/390750‑390926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑