BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,728,808	(C)_10→8	pseudogene (1254‑1255/1457 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,728,800	0	C	.	89.2%	345.4 / 35.0	93	pseudogene (1263/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (7/3); new base . (48/35); total (55/38)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	3,728,801	0	C	.	89.5%	293.6 / 49.2	93	pseudogene (1262/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (0/1); major base . (42/35); minor base G (9/0); total (55/38)
Fisher's exact test for biased strand distribution p-value = 9.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

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ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  1:2060953/140‑1 (MQ=255)

  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccg                                                                                                                                     >  1:283119/1‑139 (MQ=255)

      tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccc                                                                                                                                 <  2:2468919/140‑1 (MQ=255)

        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  1:66278/140‑2 (MQ=255)

           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  1:2042413/140‑5 (MQ=255)

           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  2:3478954/140‑5 (MQ=255)

            gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccgccgccgggcg                                                                                                                           >  2:594662/1‑135 (MQ=255)

              aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtacggt                                                                                                                         <  1:601842/140‑8 (MQ=255)

                  cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATc                                                                                                                   >  1:2683367/1‑140 (MQ=255)

                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                                <  2:2726798/140‑1 (MQ=255)

                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑T‑GGCCCCCCCGTACGGtctgggtg                                                                                                                >  1:3719690/1‑133 (MQ=255)

                     tGCGCATCTGGAAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGAACGGGAATCGAg                                                                                                                >  1:845925/1‑140 (MQ=255)

                        gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                             <  1:2153321/140‑1 (MQ=255)

                        gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                             <  1:2154597/140‑1 (MQ=255)

                         cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTGCGGTACTCGAgctgc                                                                                                            >  2:4179919/1‑140 (MQ=255)

                         cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCACCAT‑‑CCCCCCCCGGCCGGTCATCGAgctgc                                                                                                            >  2:4179970/1‑140 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                         <  2:6189442/140‑1 (MQ=255)

                                tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                     <  2:4996827/140‑1 (MQ=255)

                                tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCACCCGTACGGTAATCGAGCTTCTGACCGc                                                                                                     <  2:4058691/140‑1 (MQ=255)

                                   aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑‑CCGCCCCCGGCCGGTACTCGAGCTGCGGAgcgcggt                                                                                                  >  1:5298436/1‑137 (MQ=255)

                                     gCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                <  1:5265872/140‑1 (MQ=255)

                                       gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGAtctc                                                                                              >  2:6178623/1‑140 (MQ=255)

                                       gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCGCCCCCGGACGGGCAGCGGGCTGCTGACCGCGGAGATc                                                                                              >  2:6178832/1‑140 (MQ=255)

                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTGCGGTTATCGAGCTGGTGACCGCGGATGTcc                                                                                             >  2:598115/1‑140 (MQ=255)

                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTGCGGTGGGCGTGCTGCTGGCCGCTggggtgc                                                                                             >  1:5010870/1‑134 (MQ=255)

                                         cTGGTCTAACGGTAGTTGCGGCACCACTTCAATGAGCGATTGCTCTGGCTGCGCCGCGAGCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCA‑‑GGCGCCCCCGTACGGGAGGCGAGCGGCGGACCGCGGAGGTCCg                                                                                            >  2:2744405/1‑140 (MQ=255)

                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCGGCGCCCGGACGGGTATGGGGCTGCTGGCCGCTGATTGCCGCCAGCCTc                                                                                    >  1:166655/1‑140 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:2060475/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑‑CCCCCCCCGGACGGTGAGCGCGCTGCTGGCCGCTGGTGTCCGCCGGCAGCTgg                                                                                 >  1:4428449/1‑139 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  2:2147524/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCATTGAGCGATTTCTCTGTATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCACCCCCGTCCGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  2:1723679/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGGAGGGTAATCGAGCTGCTGACCGCTGATTTCCGCCAGGTTCTGACg                                                                               >  1:4196454/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCGGATATCCGCCCGCCGCTGACg                                                                               >  1:2797293/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  1:6088016/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAAGCGGGCTGCGGACCGCGGATATCCGCCAGCATCTGACg                                                                               >  2:2530639/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  1:2796862/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGCATCGAGCTGGGGACCGCTGATGTCCGGCAGCATCTGACg                                                                               >  1:6069459/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCATCAT‑‑CCCCCCCCGTTCGGGATTCGAGCTGCTGACCGATGATATCAGCAAGCATCTGACg                                                                               >  2:4334170/1‑140 (MQ=255)

                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:4259421/140‑1 (MQ=255)

                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTATGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:1263016/140‑1 (MQ=255)

                                                       gTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:195809/140‑1 (MQ=255)

                                                       gTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:5998939/140‑1 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  2:661602/136‑1 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 >  1:661602/1‑136 (MQ=255)

                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCAAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                           <  1:1780546/140‑1 (MQ=255)

                                                           cGGCAACAATTCAATGAGCGAATTCTCAGGATTCGCCGCGAACTGCGCCAGCCTTGATGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGCCCGCTGATCGCCGCCAGCAGCGGACGcccgg                                                                          >  2:105648/1‑137 (MQ=255)

                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAATCGAGCTGCTGTCCGCTGATGTCCGCCAGCATCTGACGCCGGTAc                                                                        >  2:5846334/1‑140 (MQ=255)

                                                                          taGCGATTTCTCTGGATTCGCAGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGTTAATCGAGCTGCTTAACGCTGATATCCGCCAGCATCTGACGCCATTACTCACTACGcgccc                                                           <  2:1432569/139‑1 (MQ=255)

                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGCGCGGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  2:1432552/140‑1 (MQ=255)

                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  2:4389696/140‑1 (MQ=255)

                                                                             cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc                                                        >  1:5349298/1‑140 (MQ=255)

                                                                             cGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAACCCCCCCCC‑‑TACGGCAATCGCGCGGCGGACCGCTGAGCTCCGCCAGCATCTGACGCCAGTACTCACGACGcgccccgc                                                        <  2:282811/140‑1 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                       <  1:6366134/140‑1 (MQ=255)

                                                                               cgtactctCGAGTCGCCCCGATCTGCGCCCGCCTTGCAGCCCCCAGTGCGGGCCCCCCAT‑‑CCCCCCACGAACGGCAATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGTACACACCACCcgccccgccc                                                      <  2:5762075/136‑1 (MQ=255)

                                                                               aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                                                      <  2:5761858/140‑1 (MQ=255)

                                                                                tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGCGCTGCTGAGCGCTGATATCCGCCAGCATCTGACGCGAGTACTCACTACGcgccccgccgc                                                     >  1:1192203/1‑138 (MQ=255)

                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  1:2031714/140‑1 (MQ=255)

                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  1:2031744/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:3461363/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:4199401/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATATGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:1076908/140‑1 (MQ=255)

                                                                                      tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGGTGACCGCTGTTATCCGCCCGCATCTGACGCCAGTACTCACGACGCGGCCCGCCCCCAATCa                                               >  2:2311624/1‑140 (MQ=255)

                                                                                        gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGTACTCGAGCTGCTGGCCGCTGATATCCGCCAGCCTCTGACGCCAGTCCGCACTACGCGCCCCGccccccagcgac                                             >  1:1287744/1‑133 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGGACTCGAGCGGCGGACCGCTGATCTGCGCCCGCGTCTGTCGCCCGTTCTCCCTACGCGGCCCGCCCCCAGTCGACGGAac                                        >  1:2195079/1‑140 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGCCGGTAATCGAGCTGCTGACCGCGGGTTTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCGCTCCACGTacg                                        >  1:2367067/1‑138 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACggacc                                        >  1:1118548/1‑136 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTGCGGTAAGCGGGCTGCTGACCGCTGATGTCCGCCAGCATCTGACGCCAGTCCGCACTACGCGCCCCGCCCCCAATCAACGTCac                                        >  1:1478134/1‑140 (MQ=255)

                                                                                             gcagcgATCTGCGCCAGCCTTGCAGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTAGGGAAATGGAGCTGCTGACCGCTGATATACGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCAGTCAACGTAac                                        >  2:2858680/4‑140 (MQ=255)

                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGGTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAGTCAACGTAacg                                       >  1:3952984/1‑139 (MQ=255)

                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATGGAGCTGCTGTGCGCTGTTATGCGCCTGCATCTGTCGCCTGGACTCACTACGCGCCCCGCGCCCAATCAACGTAACACttt                                   >  2:3762699/1‑140 (MQ=255)

                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGGAACACttt                                   >  1:2359511/1‑140 (MQ=255)

                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  >  1:555087/1‑140 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGGTGCTGACCGCTGCTATGGGGCAGCATCTGACGCCCGTGGTCACTACGCGCCCCGCCCGCAAGCGACGGAacggttgg                                  >  2:2205178/1‑134 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:2564490/140‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCGTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTg                                  <  2:6068008/140‑1 (MQ=255)

                                                                                                           cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCGGCTGACCGCTGATATCCGCCAGCTTCTGACGCCAGTACTCACTTCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                          <  2:2179825/140‑1 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCC‑ATCAACGTACCACTTTGCGGTTTAATAcc                      >  2:1054952/1‑140 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAttc                       >  1:984466/1‑138 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGGTTAATACCGCa                   >  2:3764061/1‑140 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGTTTTCGGCCTGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACGTTGCGGTTTAAGACCGCa                   >  1:1464569/1‑140 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAATCGAGCTGGTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                   >  2:3347760/1‑140 (MQ=255)

                                                                                                                   ctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCACCCGTACGGTAATCGAGCTGATGACCGCTGATATCCGCCAGCAACTGACTCCAGTACTCACTACGCGCACCGCCCCCAATCAACTTAACACTTTGCGTTTTAATACCTCAg                  <  2:3863486/140‑1 (MQ=255)

                                                                                                                      cgcCCAGTGCTGGCCCCCCAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  2:222717/140‑1 (MQ=255)

                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:594662/140‑1 (MQ=255)

                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:4333150/140‑1 (MQ=255)

                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGGACTCGTGCTGCTGGCCGCGGATCTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTGGCGGTTTAATACCGCAGGCATGCAc          >  1:6242713/1‑140 (MQ=255)

                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAAGACCGCAGGCATGccc          <  2:4936049/140‑3 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGTGCTGCTGACCGCTGATATCCGCCAGCATATGACGCCAGTACTCACTCCGCGCCCCGCCCCCATTCAACGTAACACTTTGCGGGTTATTGCCGCAGGCATGCACGa        >  1:1899180/1‑140 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGGTATCCGCCAGCATCTGTCGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGa        >  1:2349610/1‑140 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa        >  1:2349466/1‑140 (MQ=255)

                                                                                                                               cgggCCCCACAT‑‑CCCCCCCCGTACGGTAATCGTGCTGCTGACCGCGGTTATCCGCCAGCATATGACGCCAGTACTCACTACGCGCCCCGCCCCGATTCAACGGACCACTTTGCGGTTTAATACCGCAGGCATGCACGACa      <  2:1548436/138‑1 (MQ=255)

                                                                                                                                 ggCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATc    >  1:3132526/1‑140 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTACTCGCGCGGCTGTCCGCTGATAGCCGCCAGCAGGTGACGCCTGGACTCACTACGCGCCCCGCCCCCACTCAACGGAACGCTTTGCGGGTTAATACCGCAGGCATGCACGACCTcc   >  2:6429823/1‑140 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCAGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATca   >  2:2706091/1‑139 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  2:1468584/1‑140 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAAACCGCAGGAATGCACGACATcc   >  2:2432287/1‑140 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCGGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACATTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  2:525557/1‑140 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCGGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTTCGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  2:2632198/1‑140 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  1:449752/1‑140 (MQ=255)

                                                                                                                                   ccccACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa  <  2:3402561/140‑1 (MQ=255)

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GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`