BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,331,820	(G)_7→6	pseudogene (473/817 nt)	rdcB →	YjcZ‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,331,814	0	G	.	99.1%	2001.5 / 6.8	423	pseudogene (467/817 nt)	rdcB	YjcZ‑like family protein
Reads supporting (aligned to +/- strand): ref base G (1/3); new base . (171/247); total (173/250)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 438 total aligned reads displayed.

AAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331677‑4331949

aaaGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggg                                                                                                                                       <  1:4544540/140‑1 (MQ=255)

 aaGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCTAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTAGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTgggg                                                                                                                                      <  1:2435483/140‑1 (MQ=255)

  aGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggg                                                                                                                                     <  1:5913217/140‑1 (MQ=255)

      gTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggggcat                                                                                                                                 <  1:3019930/140‑4 (MQ=255)

              aaCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGGATAACGCCTGGTCGTTGCCTGGGCGCTGGTTTCTCGGTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAt                                                                                                                        >  2:5936405/1‑140 (MQ=255)

               aCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCCAATGATTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCGGTGTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtg                                                                                                                       >  2:6242540/1‑140 (MQ=255)

               aCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtg                                                                                                                       >  2:3935968/1‑140 (MQ=255)

                 gAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcc                                                                                                                     >  1:2731014/1‑140 (MQ=255)

                 gAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcc                                                                                                                     <  1:227710/140‑1 (MQ=255)

                  aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAACTTTTGACCCGCTGGCGTGCTGGGGCGGATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGTCATTTGGCGAtgcct                                                                                                                    <  2:2320507/140‑1 (MQ=255)

                  aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:3537244/140‑1 (MQ=255)

                  aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:2833045/140‑1 (MQ=255)

                  aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  2:3277987/140‑1 (MQ=255)

                  aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:1662580/140‑1 (MQ=255)

                  aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:1482690/140‑1 (MQ=255)

                        aCTGGTGCAGGCAGGTGATCAGAGCGAGCCAAGTTTTACCCGCTGGCGGGGTGGGGCGTATAGCGCATGGTCGGTGGCGGGGCGCTGTGTTCTCGTTCTGTAGGAGTTGCGCT‑GGGGGGCATTTGGCGTGGGCTGCCGtg                                                                                                              >  2:5757300/1‑139 (MQ=255)

                            gTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTggg                                                                                                          <  1:2103875/140‑1 (MQ=255)

                             tGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGa                                                                                                         >  2:1830352/1‑140 (MQ=255)

                               caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       >  1:4241096/1‑140 (MQ=255)

                                 ggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGcc                                                                                                     >  2:3745420/1‑140 (MQ=255)

                                   caggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCg                                                                                                   <  2:3431994/140‑1 (MQ=255)

                                   caggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCg                                                                                                   >  2:1340288/1‑140 (MQ=255)

                                    aggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGc                                                                                                  <  1:5179585/140‑1 (MQ=255)

                                     ggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCa                                                                                                 <  1:1759628/140‑1 (MQ=255)

                                     ggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCa                                                                                                 <  2:1232454/140‑1 (MQ=255)

                                      gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCaa                                                                                                >  1:6449152/1‑140 (MQ=255)

                                      gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCaa                                                                                                >  1:2200042/1‑140 (MQ=255)

                                      gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCaa                                                                                                >  1:4250128/1‑140 (MQ=255)

                                      gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTGGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCaa                                                                                                >  2:1121963/1‑140 (MQ=255)

                                       tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               <  1:3355129/140‑1 (MQ=255)

                                              agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                                                        >  2:1431347/1‑140 (MQ=255)

                                              agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                                                        >  2:2376315/1‑140 (MQ=255)

                                               gcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCg                                                                                       <  2:617883/140‑1 (MQ=255)

                                                cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  1:4756153/1‑140 (MQ=255)

                                                cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  2:1051256/1‑140 (MQ=255)

                                                   gcAAATTTTTCCCCGCTGGCGTGCTGGGGCGGATAGCGCCTGTTCGTTGCCTGGGCGCTGGTTGTTCGGTGTGGAGGAGTGGCGGT‑GGGGGGCTGTTGGCGAGGCCTGCCGTCTGGGGCGCCCGCGAGGGGGGGCgtggg                                                                                   >  2:4754867/1‑137 (MQ=255)

                                                     aaaTTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGttgttg                                                                                 <  1:4598007/140‑1 (MQ=255)

                                                      aaTTTTTACCAGCTGGCGTGCTGGTGCGTATAACGCCTGGTCGTTGCCTGGGCGATGTTTTATCGTTATGGAGGAGTTTAGCT‑GGGGGGCATTTTGAGATTCCTGCCGTCTGGGAAGACCGCAAGCGGTGTCGttgttga                                                                                <  2:2165168/140‑2 (MQ=255)

                                                                  cTGGCGAGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGC‑AGGGGGGCATTTGGCGATGCCTGCCGGCTGGGAAGCCCGCTAGCGGTGGCGTTGTTGCTGGGTGATTTGc                                                                    >  2:2721706/1‑140 (MQ=255)

                                                                   tGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCt                                                                   >  2:5212068/1‑140 (MQ=255)

                                                                    ggCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTc                                                                  <  1:998001/140‑1 (MQ=255)

                                                                           tGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                                                           <  1:366271/140‑1 (MQ=255)

                                                                            ggggCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGc                                                          <  1:5737203/140‑1 (MQ=255)

                                                                                cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  1:3935968/140‑1 (MQ=255)

                                                                                  tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTg                                                                          >  1:1005561/1‑118 (MQ=255)

                                                                                  tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  2:3411467/140‑1 (MQ=255)

                                                                                  gataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    >  2:1779433/2‑140 (MQ=255)

                                                                                     aaCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACacaaca                                                 >  1:5095670/1‑140 (MQ=255)

                                                                                      aCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                                                <  1:2061200/140‑1 (MQ=255)

                                                                                          cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  1:5379767/140‑1 (MQ=255)

                                                                                          cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  2:5017871/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGTCTCTGTTTTATCGGTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCAGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:5351140/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:2694654/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:2984927/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:353163/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:837794/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:1987224/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:4623203/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:4074403/140‑1 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGGTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGcc                                          >  1:84755/1‑139 (MQ=255)

                                                                                            ggTCGTTGCCTGGGCGCGGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:650054/140‑1 (MQ=255)

                                                                                            ggTCGGTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:14641/140‑1 (MQ=255)

                                                                                             gTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCag                                         >  1:1596706/1‑140 (MQ=255)

                                                                                               cGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCagag                                       <  2:2765531/140‑1 (MQ=255)

                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  1:3743663/140‑1 (MQ=255)

                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  1:4967554/140‑1 (MQ=255)

                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGAGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  2:5623379/140‑1 (MQ=255)

                                                                                                        ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              >  2:384645/1‑140 (MQ=255)

                                                                                                        ggCGCTGTTTTATCGTTCTGGAGGAGGTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              <  1:5569416/140‑1 (MQ=255)

                                                                                                        ggCGCTGTGTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              >  2:4435007/1‑140 (MQ=255)

                                                                                                         gcgcTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATg                             <  1:5816042/140‑1 (MQ=255)

                                                                                                            cTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTggg                                                                                                          >  1:4339694/1‑60 (MQ=255)

                                                                                                               ttttATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCAc                       <  1:1320928/140‑1 (MQ=255)

                                                                                                                   aTCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGAc                   <  1:6160562/140‑1 (MQ=255)

                                                                                                                    tCGTTCTGGAGGAGTTGAGCT‑GGGGGGCATATGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGAAGAGAGTATCAATGCGGCACCGAcc                  >  2:6291730/1‑140 (MQ=255)

                                                                                                                     cGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCa                 <  2:2480546/140‑1 (MQ=255)

                                                                                                                     cGTTCGGGCGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCAGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACACCATCTGGCAGAGAGTATCAATGCGGCACCGACCa                 <  2:2558301/140‑1 (MQ=255)

                                                                                                                       ttCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcg               <  1:1135236/140‑1 (MQ=255)

                                                                                                                       ttCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcg               <  1:2982273/140‑1 (MQ=255)

                                                                                                                         gtGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGACGCCCGCAGGCGGTGGCGTTGTTGGTGGGGGAGTTGCTCGTGGAGGCGTCACAACATCTGGCGGAGTGTGTCAATGCGGCACCGACCAcgcg             >  2:3644979/2‑140 (MQ=255)

                                                                                                                         cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTTGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             <  2:6376460/140‑1 (MQ=255)

                                                                                                                         cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             >  1:1121623/1‑140 (MQ=255)

                                                                                                                         cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             <  1:2477782/140‑1 (MQ=255)

                                                                                                                         cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             >  2:773506/1‑140 (MQ=255)

                                                                                                                         cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             >  1:3204309/1‑140 (MQ=255)

                                                                                                                          tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:4851916/140‑1 (MQ=255)

                                                                                                                          tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:3805390/140‑1 (MQ=255)

                                                                                                                          tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:4836506/140‑1 (MQ=255)

                                                                                                                          tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:5474859/140‑1 (MQ=255)

                                                                                                                          tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:3172363/140‑1 (MQ=255)

                                                                                                                          tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:2793919/140‑1 (MQ=255)

                                                                                                                          tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:6273284/140‑1 (MQ=255)

                                                                                                                          aggaggaggaGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:1543773/139‑1 (MQ=255)

                                                                                                                            gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  2:4291990/140‑1 (MQ=255)

                                                                                                                            gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  2:5967351/140‑1 (MQ=255)

                                                                                                                              ggagTTGCGCT‑GGGGGGCACTTGGCGATGCCTGCCGACTGGGACGCCCGCAAGCGGTGGCGTTGGTGCCGGGGGCTCGGCACGACACAGCGCCCCCACAGCTGGCCGACCGGACCACTGCGGCCCCGCCCCCGCGTCACt        <  2:5244971/140‑1 (MQ=255)

                                                                                                                               gagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTa       <  2:2271096/140‑1 (MQ=255)

                                                                                                                                 gTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAtt     <  1:2939837/140‑1 (MQ=255)

                                                                                                                                 gTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAtt     <  1:6049516/140‑1 (MQ=255)

                                                                                                                                   tGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTAc   <  2:5430747/140‑1 (MQ=255)

AAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331677‑4331949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`