BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,224,095	+GA	coding (248/249 nt)	CXP41_RS06440 →	protein YmgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,224,094	1	.	A	98.9%	2150.5 / 9.3	555	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (6/0); new base A (239/309); total (246/309)
Fisher's exact test for biased strand distribution p-value = 7.23e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	1,224,094	2	.	G	98.9%	1929.4 / 9.7	555	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (6/0); new base G (240/309); total (246/309)
Fisher's exact test for biased strand distribution p-value = 7.33e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 603 total aligned reads displayed.

TCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NZ_CP025268/1223955‑1224223

||

tCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCt‑‑t                                                                                                                                     >  2:2723668/1‑140 (MQ=255)

 cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATGTCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑a                                                                                                                                  >  1:5754347/1‑140 (MQ=255)

 cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑a                                                                                                                                  >  1:5897642/1‑140 (MQ=255)

 cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑a                                                                                                                                  >  1:2274193/1‑140 (MQ=255)

  aGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATCTCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑ag                                                                                                                                 >  2:5794612/1‑139 (MQ=255)

    gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGACTCCGTGCTCTCT‑‑T‑‑agaa                                                                                                                               >  2:6363410/1‑137 (MQ=255)

    gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaa                                                                                                                               >  1:174654/1‑137 (MQ=255)

    gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaa                                                                                                                               >  2:333193/1‑137 (MQ=255)

     cTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaac                                                                                                                              <  1:3871516/140‑5 (MQ=255)

      tttACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaact                                                                                                                             >  2:4414168/1‑135 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTa                                                                                                                         <  2:3458692/140‑1 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTa                                                                                                                         <  1:2495645/140‑1 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAg                                                                                                                       >  2:3620775/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAg                                                                                                                       >  2:3774708/1‑140 (MQ=255)

                       aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGaa                                                                                                              <  2:4255459/140‑1 (MQ=255)

                         gATGAATCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATa                                                                                                            <  2:607172/140‑1 (MQ=255)

                         gATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATa                                                                                                            <  1:1861888/140‑1 (MQ=255)

                            gAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAAc                                                                                                         <  1:4414168/140‑1 (MQ=255)

                            gAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAAc                                                                                                         >  1:4977803/1‑140 (MQ=255)

                             aaaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACa                                                                                                        <  1:196531/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTTCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:3245557/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:5337581/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:1039280/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:6213839/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:4715007/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  2:4863423/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:6305692/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:3047876/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:2151147/140‑1 (MQ=255)

                               aCCAGTGTGATTCTATCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:6023261/140‑1 (MQ=255)

                                    tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                 >  2:523104/1‑140 (MQ=255)

                                    tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                 <  2:6234325/140‑1 (MQ=255)

                                     gtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  2:279827/134‑1 (MQ=255)

                                      tgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTa                                                                                               <  2:536823/140‑1 (MQ=255)

                                           ctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCAcc                                                                                          <  1:3778662/140‑1 (MQ=255)

                                           ctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCAcc                                                                                          <  2:3125983/140‑1 (MQ=255)

                                           ctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCAcc                                                                                          <  1:2562604/140‑1 (MQ=255)

                                            tttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCa                                                                                         <  1:13/140‑1 (MQ=255)

                                              tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                       <  2:925503/140‑1 (MQ=255)

                                                  aTCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGa                                                                                   >  2:2320435/1‑140 (MQ=255)

                                                    ctctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATg                                                                                 >  2:5675677/1‑140 (MQ=255)

                                                       tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                              >  1:517862/1‑140 (MQ=255)

                                                       tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                              >  2:4691268/1‑140 (MQ=255)

                                                           tGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTg                                                                          <  1:1335042/140‑1 (MQ=255)

                                                            gCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTATTGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGa                                                                         >  2:5437181/1‑140 (MQ=255)

                                                            gCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGa                                                                         >  1:2898142/1‑140 (MQ=255)

                                                             cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:2393928/140‑1 (MQ=255)

                                                              aaaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGc                                                                       >  2:5606590/1‑140 (MQ=255)

                                                                   ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  >  1:2728762/1‑140 (MQ=255)

                                                                   ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  <  1:1647030/140‑1 (MQ=255)

                                                                    tGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCa                                                                 <  1:1175582/140‑1 (MQ=255)

                                                                    tGCAGTACATTGAAATGCATAAGAC‑ACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAg                                                                <  1:4619141/140‑1 (MQ=255)

                                                                        gTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCg                                                             >  1:997145/1‑140 (MQ=255)

                                                                          aCATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGtt                                                           >  2:3522218/1‑140 (MQ=255)

                                                                           cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                          >  1:6375360/1‑140 (MQ=255)

                                                                           cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                          >  1:6101480/1‑140 (MQ=255)

                                                                           cATTGAAATGCAAAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTATTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGATa                                                          <  2:4926688/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:4018777/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  2:2563262/140‑1 (MQ=255)

                                                                              tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                       >  1:3533874/1‑140 (MQ=255)

                                                                              tGAAATGCATAAGAATACACTAACGGATATAATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TTGAACTTGAGTAAGGTTTTAGCATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                       >  2:642032/1‑140 (MQ=255)

                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  2:1187848/140‑1 (MQ=255)

                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  2:4614345/140‑1 (MQ=255)

                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  2:512932/140‑1 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                >  2:826077/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                >  1:930979/1‑140 (MQ=255)

                                                                                      aTAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttat                                               >  1:3129945/1‑140 (MQ=255)

                                                                                         aGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTtattat                                            <  2:1312615/140‑1 (MQ=255)

                                                                                         aGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTtattat                                            <  1:5653672/140‑1 (MQ=255)

                                                                                            cTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTc                                         <  1:5470356/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                        <  2:3984334/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                        <  1:1434423/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                        <  2:2884572/140‑1 (MQ=255)

                                                                                              acacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                       <  2:1898083/140‑1 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  2:3846791/124‑1 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  1:642410/1‑140 (MQ=255)

                                                                                                acTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCa                                     <  2:2413744/140‑1 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTCTTATGTCCGCAatat                                 >  2:1655019/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  2:5904129/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  1:3696607/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTGTCTTATTATGTCCGCAatat                                 >  2:1483555/1‑140 (MQ=255)

                                                                                                    aCGGATATCAATATTAATGACAGGGCATCCGTGCTCTCTGAG‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCTCCATGCTGATGCAAAGTGAGCCTTTCTGGCGTTATGCTTTCTGATTATGTCCGCAatat                                 >  2:1116303/1‑140 (MQ=255)

                                                                                                     cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                >  1:676252/1‑140 (MQ=255)

                                                                                                     cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                >  2:2076940/1‑140 (MQ=255)

                                                                                                       gATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAg                              >  2:1282527/1‑140 (MQ=255)

                                                                                                       gATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAg                              <  2:4092272/140‑1 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                            >  1:4193959/1‑140 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                            >  2:1542036/1‑140 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCG‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATAAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                            >  1:2788451/1‑140 (MQ=255)

                                                                                                           tCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtgt                          <  1:3597403/140‑1 (MQ=255)

                                                                                                             attattAATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                 <  1:843847/67‑1 (MQ=255)

                                                                                                             aatattaATGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTCAGGGTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCAGTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCa                        >  2:713096/3‑140 (MQ=255)

                                                                                                                   aaTGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATg                  <  2:4977803/140‑1 (MQ=255)

                                                                                                                    aTGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATgg                 <  1:3442416/140‑1 (MQ=255)

                                                                                                                     tGACAGGGAATCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGga                <  2:2228819/140‑1 (MQ=255)

                                                                                                                             aaTCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtctc        <  2:2021789/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg       <  1:4806515/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg       <  2:174654/140‑1 (MQ=255)

                                                                                                                                ccGTGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGct     <  1:762310/140‑1 (MQ=255)

                                                                                                                                   tGCTCTCT‑‑TAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc  >  1:4502389/1‑140 (MQ=255)

||

TCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NZ_CP025268/1223955‑1224223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`