BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	95.1%	1254.5 / 48.0	350	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (10/7); new base T (213/119); total (223/127)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 391 total aligned reads displayed.

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

cAACGACAGCGAACCGTGGGCGTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCg                                                                                                                                            >  1:1714724/1‑140 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑tt                                                                                                                                  >  2:5602964/1‑140 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑tt                                                                                                                                  >  1:1772755/1‑140 (MQ=255)

      cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑ttt                                                                                                                                 >  2:1557555/1‑139 (MQ=255)

      cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑ttt                                                                                                                                 >  1:5912025/1‑139 (MQ=255)

       cgCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑a‑t‑t‑ttta                                                                                                                                <  2:5391521/139‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑ttta                                                                                                                                <  1:3373622/140‑3 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTCCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑tttacg                                                                                                                              >  2:872387/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAATACAAATGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑tttacg                                                                                                                              >  2:6405070/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑tttacg                                                                                                                              >  1:1844855/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑t‑t‑t‑tttacg                                                                                                                              <  1:4423516/140‑5 (MQ=255)

              cGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGtt                                                                                                                          >  1:513802/1‑140 (MQ=255)

               gTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTta                                                                                                                         <  2:2415562/140‑1 (MQ=255)

                 gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTtata                                                                                                                       >  1:5508755/1‑140 (MQ=255)

                 gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTtata                                                                                                                       >  1:2095466/1‑140 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAgc                                                                                                                   <  1:1168213/140‑1 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAgc                                                                                                                   <  2:3649403/140‑1 (MQ=255)

                        agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCa                                                                                                                >  2:4013761/1‑140 (MQ=255)

                            gCGGCAACACAGGCGTAGCATACAGTTGGCACATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGGTATAAAGCGCAAAtt                                                                                                            >  2:4470935/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTa                                                                                                           <  2:1075779/140‑1 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTaaa                                                                                                         >  1:4989789/1‑140 (MQ=255)

                                   cacaGGCGTAGCATCCAGTGGGCAACTGTAGTACGGGGGGCATATGACCTGGTTTAGTGACCGGCGTCTGAACTGCAAAAACAAAACCATGGTGAATCGCCAGCGA‑T‑T‑T‑TTACGCCGTTATAACGCGCAAATTAAACAGt                                                                                                     <  2:1746308/140‑1 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   <  1:5388519/140‑1 (MQ=255)

                                      aGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGa                                                                                                  <  1:2373646/140‑1 (MQ=255)

                                       ggCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGAt                                                                                                 >  2:1940920/1‑140 (MQ=255)

                                       ggCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGAt                                                                                                 >  2:718724/1‑140 (MQ=255)

                                           tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  1:892011/140‑1 (MQ=255)

                                             tcATACAGGTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACCGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑T‑T‑TATTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  2:5993761/139‑1 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           >  1:1112715/1‑140 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  2:4085517/140‑1 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  1:128231/140‑1 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           >  1:3219232/1‑140 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  1:3693918/140‑1 (MQ=255)

                                              cATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTCTAAAGCGCAAATTAAACAGTCGATCTCCGAg                                                                                          >  2:2208444/1‑140 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:1051036/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  2:2895211/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:6296871/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:1618681/140‑1 (MQ=255)

                                                 aCAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                                       <  2:3731175/140‑1 (MQ=255)

                                                 aCAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                                       <  2:3828034/140‑1 (MQ=255)

                                                    gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTTTTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑T‑TGT‑GTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                    >  2:3675099/1‑140 (MQ=255)

                                                    gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGACTCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                    >  2:177366/1‑140 (MQ=255)

                                                     ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATta                                                                                   >  2:5173615/1‑140 (MQ=255)

                                                     ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTGATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATta                                                                                   >  2:5470616/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:6325578/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:2571252/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGAAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  1:4498353/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                <  1:2005249/140‑1 (MQ=255)

                                                          aaaTGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                              >  1:3455012/1‑140 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                         >  1:4113609/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:3083958/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:1526908/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGGTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:2029673/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  2:4360900/1‑140 (MQ=255)

                                                                           cATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGt                                                             >  1:1472397/1‑140 (MQ=255)

                                                                            atatGTCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACTAATGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGGTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGACATTAATAAGCGTTCGGTGTCTGACGTc                                                            >  2:4996835/1‑140 (MQ=255)

                                                                             tatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCg                                                           >  1:3396361/1‑140 (MQ=255)

                                                                               tGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATACGCGTTCGGTGACTGACGTCGAc                                                         <  1:2049886/140‑1 (MQ=255)

                                                                               tGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGAc                                                         <  1:4359695/140‑1 (MQ=255)

                                                                               tGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGAc                                                         <  1:5154974/140‑1 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTCTAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAAGATGCGTTCGGTGACTGACGTCGACTgcggc                                                   >  2:787602/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  2:2668395/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   <  1:4787729/140‑1 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  1:6053041/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGACAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  1:267226/1‑140 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:3831989/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  1:2154129/1‑140 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:1177898/140‑1 (MQ=255)

                                                                                        ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGaa                                                >  1:2929229/1‑140 (MQ=255)

                                                                                          aTTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                              <  2:2948138/140‑1 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:5783995/1‑140 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             <  2:1820906/140‑1 (MQ=255)

                                                                                            tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                            >  1:2739952/1‑140 (MQ=255)

                                                                                                cGGGGTCGGACCGGCAAAAACAAAAGCATGGTGCATCGCCAGCG‑‑TGT‑T‑GTACGCCGTTATACAGCGCCACTTAAACAGTCGAGCTCCGAGGCCATTAATGAGCGTTCGGTGCGTGACGTCGACTGCGGCGACTCCGTagc                                        >  2:664402/1‑138 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:3126269/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:2093302/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:5582644/1‑140 (MQ=255)

                                                                                                 ggCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:5885735/1‑140 (MQ=255)

                                                                                                 ggCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATcc                                       >  2:5293868/1‑140 (MQ=255)

                                                                                                   cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     >  2:3512292/1‑140 (MQ=255)

                                                                                                   cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     >  2:4541819/1‑140 (MQ=255)

                                                                                                     tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                   >  2:3597751/1‑140 (MQ=255)

                                                                                                       tGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTccc                                 <  1:6514/140‑1 (MQ=255)

                                                                                                       tGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTccc                                 <  1:772530/140‑1 (MQ=255)

                                                                                                        gAACGGCAAAAACAACAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCTTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  2:4794462/140‑1 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  1:4892061/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  2:1457174/140‑1 (MQ=255)

                                                                                                             gCAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                           >  2:1258345/1‑140 (MQ=255)

                                                                                                              cAAAAACAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                          >  1:4641233/1‑140 (MQ=255)

                                                                                                               aaaaaCAAAAGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                         <  1:6325579/140‑1 (MQ=255)

                                                                                                               aaaaaCAAAACCAGGGAGAATCGCCAGCG‑‑TAT‑T‑ATACGCCGTTATAAAGCGCAAAGTCAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGCCGTCGACAGCGGCGAATCCGTATCCATTCCCCCGGAAGa                         <  2:3478943/140‑1 (MQ=255)

                                                                                                                        aGCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGa                >  2:3939644/1‑140 (MQ=255)

                                                                                                                         gCATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGaa               <  2:3184198/140‑1 (MQ=255)

                                                                                                                          cATGGTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAc              <  1:244211/140‑1 (MQ=255)

                                                                                                                              gTGAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATGAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt          <  2:1668069/140‑1 (MQ=255)

                                                                                                                                gAATCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCTCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  1:5227101/140‑1 (MQ=255)

                                                                                                                                   tCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATCAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtc     >  2:1130772/1‑140 (MQ=255)

                                                                                                                                   tCGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtc     >  2:48994/1‑140 (MQ=255)

                                                                                                                                    cGCCAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              >  2:6276263/1‑114 (MQ=255)

                                                                                                                                      ccAGCGT‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATAGTAGCGAACCGATGTGtcatc  >  1:976383/1‑140 (MQ=255)

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`