BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,033,768	(C)_5→6	pseudogene (190/920 nt)	yedA →	drug/metabolite exporter YedA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,033,763	1	.	C	97.0%	1455.6 / 34.0	429	pseudogene (185/920 nt)	yedA	drug/metabolite exporter YedA
Reads supporting (aligned to +/- strand): ref base . (8/5); new base C (204/212); total (212/217)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 480 total aligned reads displayed.

TGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑CC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NZ_CP025268/2033625‑2033897

 gTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTCTTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑cc                                                                                                                                        >  2:5922075/1‑140 (MQ=255)

 gTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑cc                                                                                                                                        >  1:6210400/1‑140 (MQ=255)

  tataTCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑cc‑c                                                                                                                                      <  1:3318612/140‑1 (MQ=255)

   atatCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑cc‑cc                                                                                                                                     >  2:1505877/1‑140 (MQ=255)

   atatCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑cc‑cc                                                                                                                                     >  1:4230941/1‑140 (MQ=255)

   atatCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑cc‑cc                                                                                                                                     >  1:6189177/1‑140 (MQ=255)

        aTTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑cc‑ccccgct                                                                                                                                <  2:1902035/140‑5 (MQ=255)

          ttGGGGCTCACCCTATTTGGTCATGCGGATTGGCGTGGAAAGCTGGCCGCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTAc                                                                                                                              <  2:6315026/140‑1 (MQ=255)

          ttGGGGCTCAACCTATTTGGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTAc                                                                                                                              <  1:981370/140‑1 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  1:3210380/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  1:6303343/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  1:3154392/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  2:394704/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  1:5554517/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  2:5037483/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  1:5855028/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  2:5381866/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  2:4377053/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGAAACTAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  2:1032054/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGAGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  2:6383593/1‑140 (MQ=255)

            ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTAAATGATGTCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGt                                                                                                                            >  2:2134608/1‑140 (MQ=255)

             gggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTc                                                                                                                           <  2:1248948/140‑1 (MQ=255)

               gCTCATCCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCg                                                                                                                         <  1:1938508/140‑1 (MQ=255)

                   aaCCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGAGGGCGTTCGATTCATGGCGGCCGGGATGTTATTGCTGGCATTTGTGCTTCTGCGCGGAGACAATC‑TCCC‑CCC‑GCTAGGTCCgctgc                                                                                                                     >  2:5649828/1‑140 (MQ=255)

                    aCCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCgctgct                                                                                                                    <  1:3095851/140‑1 (MQ=255)

                         ttttGTCAGTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATg                                                                                                               <  2:4453115/140‑1 (MQ=255)

                             gTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCcgc                                                                                                           >  2:1573407/1‑140 (MQ=255)

                               cATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCgcgc                                                                                                         >  1:4055609/1‑140 (MQ=255)

                                aTTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCt                                                                                                        >  2:3020622/1‑140 (MQ=255)

                                   cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                     >  2:2963279/1‑140 (MQ=255)

                                   cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                     <  1:2036847/140‑1 (MQ=255)

                                     gATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTg                                                                                                   <  2:65790/140‑1 (MQ=255)

                                      aTTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  <  2:1091074/140‑1 (MQ=255)

                                        tGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGcc                                                                                                >  2:307342/1‑140 (MQ=255)

                                        tGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGcc                                                                                                >  2:3632420/1‑140 (MQ=255)

                                         ggCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCt                                                                                               >  2:5241223/1‑140 (MQ=255)

                                          gCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTg                                                                                              <  1:1147059/140‑1 (MQ=255)

                                           cGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGt                                                                                             >  2:1687665/1‑140 (MQ=255)

                                                aaaGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTg                                                                                        >  1:2388189/1‑140 (MQ=255)

                                                    cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    <  1:3428879/140‑1 (MQ=255)

                                                    cTGGCCTACGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTCTTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCCATGCCGCGCTGATTGGCGTGGTATTgctgg                                                                                    >  2:4965264/1‑140 (MQ=255)

                                                     tGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggc                                                                                   >  1:1021003/1‑140 (MQ=255)

                                                     tGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggc                                                                                   <  1:918213/140‑1 (MQ=255)

                                                     tGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggc                                                                                   >  1:4857371/1‑140 (MQ=255)

                                                      ggCCTCCGTTACTGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggct                                                                                  <  1:3665070/140‑1 (MQ=255)

                                                        ccTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                <  2:3157082/140‑1 (MQ=255)

                                                          tCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCg                                                                              >  2:4823567/1‑140 (MQ=255)

                                                            cGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTAGGTGATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCGACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGt                                                                            >  2:5089355/1‑140 (MQ=255)

                                                                      ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATgg                                                                  >  2:2810360/1‑140 (MQ=255)

                                                                      ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATgg                                                                  >  1:765191/1‑140 (MQ=255)

                                                                      ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATgg                                                                  >  2:910014/1‑140 (MQ=255)

                                                                              ttCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGtt                                                          >  1:4372191/1‑140 (MQ=255)

                                                                                  aTTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCg                                                      <  1:1664154/140‑1 (MQ=255)

                                                                                   ttCCTGGCAGCCGGTATTTTATTGCTGGCAATTGAGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGAGTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGa                                                     <  2:3626659/140‑1 (MQ=255)

                                                                                      cTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACa                                                  <  1:4730272/140‑1 (MQ=255)

                                                                                        ggCAGCCGGTATTTTATTGCTGGCATTATTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGACCGCTGCTCAATGCCGCGCTGATTGGCCTGATATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATc                                                >  2:5559136/1‑140 (MQ=255)

                                                                                                       aTTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCg                                 >  1:5466720/1‑140 (MQ=255)

                                                                                                        ttGCTGGCAGTTTTGCTACTGCGCGGACACCAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCgg                                >  2:1360002/1‑140 (MQ=255)

                                                                                                         tGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGc                               >  1:1217535/1‑140 (MQ=255)

                                                                                                           cTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAATCTGTTCCTTCCGGCag                             >  2:3843529/1‑139 (MQ=255)

                                                                                                           cTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             >  1:3596506/1‑140 (MQ=255)

                                                                                                           cTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  1:4316346/140‑1 (MQ=255)

                                                                                                           cTGGCATTTGTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGAGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  1:568579/140‑1 (MQ=255)

                                                                                                           cTGGCATTATTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCACTGCCGCGCTGATGGCCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  2:2265853/140‑1 (MQ=255)

                                                                                                             ggCATTTTTGCTACTGCGCGGACACAAACGC‑CC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGACCATCAAAATGTTCCTTCCGGCATcg                           <  2:4238860/140‑1 (MQ=255)

                                                                                                             ggCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           >  1:2995742/1‑140 (MQ=255)

                                                                                                             ggCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           >  2:5807943/1‑140 (MQ=255)

                                                                                                             ggCATTTTTGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           <  1:5242224/140‑1 (MQ=255)

                                                                                                                 tttttGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcgccgc                       <  1:4499787/140‑1 (MQ=255)

                                                                                                                 tttttGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcgccgc                       <  2:3355497/140‑1 (MQ=255)

                                                                                                                   tttGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGccgccg                     <  1:2576265/140‑1 (MQ=255)

                                                                                                                     tGCTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  1:5037483/140‑1 (MQ=255)

                                                                                                                     tGCTACTGCGCGAACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  1:63568/140‑1 (MQ=255)

                                                                                                                       cTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGt                 <  2:3278016/140‑1 (MQ=255)

                                                                                                                       cTACTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGt                 <  2:6031030/140‑1 (MQ=255)

                                                                                                                         aCTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   >  1:4622208/1‑136 (MQ=255)

                                                                                                                          cTGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGt              >  1:2878546/1‑140 (MQ=255)

                                                                                                                           tGCGCGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCGGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGtt             >  1:2711419/1‑140 (MQ=255)

                                                                                                                            gcgcGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTg            >  2:5325681/1‑140 (MQ=255)

                                                                                                                            gcgcGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTg            >  2:1940433/1‑140 (MQ=255)

                                                                                                                            gcgcGGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTg            >  1:3825389/1‑140 (MQ=255)

                                                                                                                             cgcgGACACCCAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGAGTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAACATGTTCCTTACGGCATCGCCGCCGTAGTGGTTGc           <  2:2391914/140‑1 (MQ=255)

                                                                                                                             cgcgGACACAATC‑T‑CCGCCC‑GCTACGTCCGCTGCTCTATGCCGCGCTGAGTGGGCTGTTATTGATGGCTGTCGGTGCTGGCATGGTGACGGTTGCCGACCCTCTAAATGTGCCTTCCGGGAGCGCCGCGGTAGTGGGTGc           >  2:3049734/1‑140 (MQ=255)

                                                                                                                             cgcgGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGc           >  1:6095309/1‑140 (MQ=255)

                                                                                                                               cgGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAATTGTTCCTTCCGGCCTCGCCGCCGTAGTGGTTGCaa         >  2:548865/1‑140 (MQ=255)

                                                                                                                               cgGACACAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCaa         >  2:4647333/1‑140 (MQ=255)

                                                                                                                                  acacAAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCg      >  2:6260205/1‑140 (MQ=255)

                                                                                                                                    acaAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCggg    <  2:4062194/140‑3 (MQ=255)

                                                                                                                                    acaAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    <  2:1189594/140‑1 (MQ=255)

                                                                                                                                    acaAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    <  1:2134608/140‑1 (MQ=255)

                                                                                                                                    acaAAC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    >  2:2573277/1‑140 (MQ=255)

                                                                                                                                      caaC‑T‑CC‑CCCAGCTACGTCCGCTGCTCATTGCCTCGCTGATTGTCCTGTTATTGGTTGCTGTCGGTAATTGCATGTTGAAGGTTGCCGAACATCTAAATGTTCCTTACGGAATCGCCTCCGTAGTGGTTGCAACCGTgag  <  2:4193309/139‑3 (MQ=255)

                                                                                                                                      aaaC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:3986254/140‑1 (MQ=255)

                                                                                                                                      aaaC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:4932565/140‑1 (MQ=255)

                                                                                                                                      aaaC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:2755548/140‑1 (MQ=255)

                                                                                                                                      aaaC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:1551763/140‑1 (MQ=255)

                                                                                                                                      aaaC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:5381887/140‑1 (MQ=255)

                                                                                                                                      aaaC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:5847080/140‑1 (MQ=255)

                                                                                                                                      aaaC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:2809794/140‑1 (MQ=255)

                                                                                                                                      aaaC‑TCCC‑CCC‑GCTACGTCCGCTGCTCAAGGCCGCGCTGATTGGCCTGTTATTGCGGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:880578/140‑1 (MQ=255)

TGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑CC‑CCC‑GCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NZ_CP025268/2033625‑2033897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`