BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,466,923	(C)_8→9	intergenic (+242/‑123)	fadL → / → CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,466,915	1	.	C	93.1%	509.4 / 31.2	161	intergenic (+234/‑131)	fadL/CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (0/11); new base C (14/135); total (14/147)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATG  >  NZ_CP025268/2466778‑2467048

ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cc                                                                                                                                       >  1:4536724/1‑140 (MQ=255)

 ttcAAACAGCCTGTTTGCAGCTTGCACTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGTCATATTTTCAGGCGTTCGGCTCGTCCTTCTCAAAGAGTTTACTATTCTGCATTTCCAGGATACT‑‑‑ccc                                                                                                                                      <  2:2492857/138‑1 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑ccc                                                                                                                                      <  1:3069221/140‑1 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑ccc                                                                                                                                      <  2:2187447/140‑1 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑ccc                                                                                                                                      <  1:4453272/140‑1 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTGGCCCTTTCAAATCAATACGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑ccc                                                                                                                                      <  2:5235911/140‑1 (MQ=255)

  gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc                                                                                                                                     <  1:5354212/140‑1 (MQ=255)

  gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAACCGGTATCCACAGACAAACCTGACCGCACAGGCATATTTTCAGGCGTTCGGCTCGTCCTTCACAAAGAGTTTACTTTTCTGCATATCCAGGATACT‑‑‑cccc                                                                                                                                     <  2:3407865/140‑1 (MQ=255)

   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑c                                                                                                                                   <  1:6222439/140‑1 (MQ=255)

     aaCCGCCTGTAGGCAGCTTGCCCATACACTTCAATACCCGGTATCCACAGACAAACCTGAAGGGAACGTCATAATTTCAGGCGCACAGCTCGTCCTTCTCAAACAGTGTACGTTTCTGCAGTGCCCGGATACG‑‑‑cccc‑ccc                                                                                                                                 <  2:3001452/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATTAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccc                                                                                                                                 <  1:3911182/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccc                                                                                                                                 <  1:473038/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccc                                                                                                                                 <  1:3380917/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccc                                                                                                                                 <  1:6182814/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccc                                                                                                                                 <  1:3316996/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccc                                                                                                                                 <  1:3242670/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTACTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccc                                                                                                                                 <  2:5477614/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATACACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑ccac‑ccc                                                                                                                                 <  2:5366568/140‑1 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATACGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAATAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccccc                                                                                                                               <  2:5602179/140‑2 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccccc                                                                                                                               <  1:104540/140‑2 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAAGAAGCGGTATCCACAGACAAACCTGAAGGGATAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccccc                                                                                                                               <  2:314523/140‑2 (MQ=255)

          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccccctgg                                                                                                                            <  1:6263887/140‑5 (MQ=255)

           cTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccccctggc                                                                                                                           <  1:1989596/140‑6 (MQ=255)

               tGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTAtt                                                                                                                       <  1:4239457/140‑1 (MQ=255)

               tGGCAGCTTGCCCTATCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTTCTCGTCATTCTCAAAGAGTTTACTTTTATGCATTTCCAGGATACT‑‑‑CCCCACCCC‑TGGCTAtt                                                                                                                       <  1:467134/140‑1 (MQ=255)

                 gCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑CCCC‑CCCC‑TGGCTATtgtg                                                                                                                    <  1:2815554/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGATGGGAAAGGCATATTTTCAGCCGTTCTGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCAGTATACTC‑‑CCCC‑CCCC‑TGGCTATtgtg                                                                                                                    <  2:1315505/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATtgtg                                                                                                                    <  1:1231279/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATtgtg                                                                                                                    <  1:3261578/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATtgtg                                                                                                                    <  2:1645097/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATtgtg                                                                                                                    <  1:1565744/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATtgtg                                                                                                                    <  1:3058401/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATtgtg                                                                                                                    <  1:1499335/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑GGGCTATtgtg                                                                                                                    <  1:5247666/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTGCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTgcg                                                                                                                  <  2:4024285/140‑1 (MQ=255)

                    gCTAGCCCATTCAACTCACTAAGCGGTATCCACAGACACAGCTGCAGGGAAAGGCATCATTTCTGGCTGTCTGCTCGTCCTTCTCAAAGAGTTTACTATGCAGCTTTTCCAGGATACTC‑‑CCCC‑CCCC‑TCGCTATTGTgct                                                                                                                  <  2:5629318/140‑2 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCa                                                                                                              <  1:3797162/140‑1 (MQ=255)

                             ttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccccc                                                                                                                               <  2:2319610/118‑2 (MQ=255)

                             ttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccccc                                                                                                                               >  1:2319610/1‑117 (MQ=255)

                             ttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACt                                                                                                        <  1:2427171/140‑1 (MQ=255)

                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACt                                                                                                        <  1:974610/140‑1 (MQ=255)

                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACt                                                                                                        <  1:2189571/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                       <  1:5350655/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                       <  1:5972413/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                       <  1:23697/140‑1 (MQ=255)

                                  aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCaaa                                                                                                    >  2:3200855/1‑140 (MQ=255)

                                  aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCaaa                                                                                                    >  2:6264536/1‑140 (MQ=255)

                                     aaTAAGCGGTATCCACATACAAACCTGAAGGTAAAGGCATATTTTCCGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATGGTGCGCTCATACACTCAAATTa                                                                                                 <  2:3477426/140‑1 (MQ=255)

                                     aaTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTa                                                                                                 <  1:2846807/140‑1 (MQ=255)

                                      aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑AGGCTCTTGTGCGCTCATACACTCAAATTaa                                                                                                >  1:6323042/1‑140 (MQ=255)

                                          gCGGTATCCACATACAAACCTGATGGGAAAGGCATATTTTAAGGCGTTCTGATCGTCCTTCTAAAAGAGTTTACTTTTCTGAATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:1824891/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAACCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:1926935/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:3909125/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:3870468/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:2694939/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:551112/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTAAAATTAAAGAt                                                                                            <  2:720537/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑GGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:5171483/140‑1 (MQ=255)

                                          gCGGTATCCACAGAAAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:1222223/140‑1 (MQ=255)

                                             gTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTTTTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                         <  1:3293758/140‑1 (MQ=255)

                                               aTCCACATACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGATTTTTCTTTTTTGCATTTCCAGGATACTCCCCCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                         <  1:1522747/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                       <  2:580701/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                       <  1:2092785/140‑1 (MQ=255)

                                                       aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataa                                                                               <  1:1128953/140‑1 (MQ=255)

                                                         aaaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTaaataaat                                                                             <  1:3767831/140‑1 (MQ=255)

                                                            ccTGAAGTGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCTGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTTAAGATAGGTTCTAAATAAATGAg                                                                          <  2:5982438/140‑1 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                          <  1:602584/140‑1 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                          <  2:4536724/140‑1 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                          <  1:4784691/140‑1 (MQ=255)

                                                                      caGGCATCTGGTCAGGCGTGCTGCACGGCCGTCTCACAGAGTTTACTTTTCTGCATGTCCAGGCCACT‑‑‑CCCC‑CCCCATGGCTATTGTGCGCTCATACACTCAAATTCAAGATAGGTTCTAAAGACAGGCGCGTATGTTGa                                                                <  2:3636219/139‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  1:6404819/140‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  1:3540497/140‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  2:1910170/140‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  1:568891/140‑1 (MQ=255)

                                                                      aaGGCATATTGTCAGGCGTTCTGCTCGTCCTTCTCAACGAGTTTACTGTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  2:4805951/140‑1 (MQ=255)

                                                                      aaGCCATATTTTCAGGCGTTCTGCTCGTCCTTCACAAAGAGTTTACTTTTCGGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  1:489591/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccccc                                                                                                                               >  2:442812/1‑75 (MQ=255)

                                                                        ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                              <  1:5430352/140‑1 (MQ=255)

                                                                          cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGATTACTTTTCTGCATTTCAAGGATACTA‑‑CCCC‑CCCC‑TGGCTATTGTGCGATAATACTCTCAAAATAAAGATAGGTTCTAAATACATGAGCGTTTTTTGATAGt                                                            <  2:5576199/140‑1 (MQ=255)

                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                         <  1:1795421/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:695697/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:1708112/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCAAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGAGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCTTTTTTTGATAGTCTATTTCa                                                    <  2:1505186/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑cccc‑ccc                                                                                                                                 >  1:562471/1‑62 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCTAAGAGTTTACTTTTCTGCATTTCCAGTATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCAATTTCAtt                                                  <  2:4692468/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCACAGAGTTTACTTGTCTGCATGTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCAATGGTGCGCACATACACTCAAATTAAAGATAGGTTCTAACTAAATGAGCGTTTTTGGATAGTCTATTTCAtt                                                  <  2:2698870/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  1:2474567/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  1:5623683/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑GGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  2:3262226/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTAATTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  2:2045713/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCACGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  2:2768189/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCACGACCTTCTCAAAGAGTTAACTTTTCGCCATTCCCAGCATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCACATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCagt                                                  <  2:2698300/140‑3 (MQ=255)

                                                                                      cGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg                                                <  2:3087896/140‑1 (MQ=255)

                                                                                       gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                               <  1:3200855/140‑1 (MQ=255)

                                                                                       gTTATGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                               <  2:6141100/140‑1 (MQ=255)

                                                                                                          aaGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAaaaa                            >  2:6442913/1‑140 (MQ=255)

                                                                                                          aaGAGTTTACTTTTCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACaa                            >  2:6443807/1‑140 (MQ=255)

                                                                                                                     tttCTGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                 <  1:2034606/140‑1 (MQ=255)

                                                                                                                         tGCATTTCCAGGATACTC‑‑CCCC‑CCCC‑TGGCTATTGTGCTCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGa             >  2:53291/1‑140 (MQ=255)

                                                                                                                                    gATACTC‑‑CCCC‑CCCC‑TGTCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATg  <  2:1707257/140‑1 (MQ=255)

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑‑CCCC‑CCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATG  >  NZ_CP025268/2466778‑2467048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`