BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,769,852	+C	intergenic (‑52/‑89)	yfjM ← / → rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,769,848	1	.	C	97.7%	1029.9 / 14.5	306	intergenic (‑48/‑93)	yfjM/rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA
Reads supporting (aligned to +/- strand): ref base . (2/5); new base C (113/184); total (115/191)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

CTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTG‑C‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NZ_CP025268/2769711‑2769982

cTTGTCTGCTGGCGAATAATTACTCAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCCTTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGT‑GTG‑c‑c                                                                                                                                       <  2:3814689/140‑1 (MQ=255)

  tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTG‑c‑ccc                                                                                                                                     <  2:5504110/140‑1 (MQ=255)

       gctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTG‑c‑cccctatt                                                                                                                                >  2:5410275/1‑135 (MQ=255)

              aataatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTgg                                                                                                                         <  1:829835/140‑1 (MQ=255)

                     aCTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATGGTTGGA‑GTGCC‑CCCT‑AGTCAGGTGGCTTAAtt                                                                                                                  >  1:5054162/1‑140 (MQ=255)

                        aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAg                                                                                                               <  1:1228380/140‑1 (MQ=255)

                        aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAg                                                                                                               <  2:2916860/140‑1 (MQ=255)

                        aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAg                                                                                                               <  1:3543554/140‑1 (MQ=255)

                           tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGGCCCCCCGGTAGGCGATCCTGCGGTATTGTTGGA‑GTGCC‑CCCG‑ATTCAGGTGGCTAAATTTAGCaa                                                                                                            >  2:1415346/1‑140 (MQ=255)

                             tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          <  2:4426531/140‑1 (MQ=255)

                                aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTAGAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCaa                                                                                                       >  1:3400916/1‑140 (MQ=255)

                                 gTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAAt                                                                                                      <  1:3670218/140‑1 (MQ=255)

                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:4921584/140‑1 (MQ=255)

                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  2:5703658/140‑1 (MQ=255)

                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:1657446/140‑1 (MQ=255)

                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑CTTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                  >  2:2351327/1‑140 (MQ=255)

                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                  >  1:245206/1‑140 (MQ=255)

                                        ctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTc                                                                                               <  1:779797/140‑1 (MQ=255)

                                        ctTGGTGAGATCACGAAGAGGTTACCGAAACGATGGACATCATCCCCCATATTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATACTTGGA‑GTGCC‑CCCT‑ATACAGGTGGCTAAATATAGCAAATCAATACACTTc                                                                                               <  2:3049794/140‑1 (MQ=255)

                                         ttGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                                                              <  1:882125/140‑1 (MQ=255)

                                          tGGTGAGATCAGGCAGAGGTTACCGAAACGATGGACATCCTCCCCCATTTTGTGGACGGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATGTAGCAAATCAATACACTTCAg                                                                                             <  2:1657154/140‑1 (MQ=255)

                                          tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                             <  1:4131676/140‑1 (MQ=255)

                                            gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  1:1292782/140‑1 (MQ=255)

                                            gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  1:2777519/140‑1 (MQ=255)

                                            gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  2:1089930/140‑1 (MQ=255)

                                            gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  2:490379/140‑1 (MQ=255)

                                             tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg                                                                                          <  2:4212861/140‑1 (MQ=255)

                                               agaTCAGGAAGTGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGTCGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  2:476123/140‑1 (MQ=255)

                                               agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  1:11386/140‑1 (MQ=255)

                                               agaTCAGGAAGAGGTTACCGAAACGATGGACAACATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  2:4694894/140‑1 (MQ=255)

                                                 aTCAGGAAGATGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑CTTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  1:2282780/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  1:1557078/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  1:462099/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  1:5623102/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  1:5186659/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  2:4033136/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑AGTCAGGGGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      >  2:5573744/1‑140 (MQ=255)

                                                 aTCAGGAAGAGGTTACAGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGACCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  2:1926741/140‑1 (MQ=255)

                                                  tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     <  2:2275582/140‑1 (MQ=255)

                                                  tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     >  2:748571/1‑140 (MQ=255)

                                                      gAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTAGAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACGTCAGGGGGGtattatg                                                                                 >  1:4975375/1‑139 (MQ=255)

                                                       aaGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTg                                                                                <  1:3811667/140‑1 (MQ=255)

                                                         gagGGTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTG‑CGCCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTa                                                                              <  1:5372203/140‑1 (MQ=255)

                                                           ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTCACTTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            >  2:3261243/1‑140 (MQ=255)

                                                           ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTATATTTAGCAAAACAATACACTTCAGGGGGGTATTATTGgaga                                                                            >  1:6433982/1‑140 (MQ=255)

                                                           ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            >  1:4828800/1‑140 (MQ=255)

                                                           ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGATTATTGTaga                                                                            >  1:4194003/1‑140 (MQ=255)

                                                           ggTTACCGAAACGATGGACATCATCCCCCATGTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            >  1:1485196/1‑140 (MQ=255)

                                                           ggTTACCGAAACGATGGACATAATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTACATGTAGCAAATCAATACACGTCAGGGGGGTAGTATTGTagt                                                                            >  2:2578654/1‑139 (MQ=255)

                                                             ttACAGAAACGATGGACATCATCACCAATGTTTTTGTCTGGACCCAATGTAGTCTATCATGCCCTATTGTTGGATTTT‑C‑CACT‑ATTTAGTGGTCTATTTTTAGCAAATAAATACAATTATGGTGGGTATTCTTGTagttt                                                                          <  2:5302886/140‑4 (MQ=255)

                                                                ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGGTGGA‑GTGCC‑CCCT‑ATGCAGGTGGCTAAATTTAGCAAATCAATACCCTTCAGGGGGGTATTATTGTAGAGTTTc                                                                       >  2:5071478/1‑140 (MQ=255)

                                                                 cGAAACTATGGACATCATCCCTCATTTTGTGGAATGCCCCCCCGTTAGACGATACTGCCCTAAATTTGGA‑GTGCC‑CCCT‑AGTCAGGTGTCTAAATTTAGCAAATCAAGACACTTCAGTGGGGTATTATTGTAGAGTTTcc                                                                      <  2:4842768/140‑1 (MQ=255)

                                                                 cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                      <  1:5439205/140‑1 (MQ=255)

                                                                      cGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                                                                 <  1:1778870/140‑1 (MQ=255)

                                                                       gATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCagag                                                                >  1:6320263/1‑137 (MQ=255)

                                                                        aTGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATg                                                               >  1:2537062/1‑140 (MQ=255)

                                                                                catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCAGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTagg                                                       >  2:1739968/1‑138 (MQ=255)

                                                                                catcCCCCATTCAGTGGACTGCCCCCCCGGTAGACGATCCAGCCCTATAGTTGGA‑GTGCC‑CCCT‑AGTCAGGTGGCAAAATTTAGCAAATCAATAAACGTCCGGGGGGTATTATTGTAGAGATTCCCCATATGTTTCTATg                                                       <  2:582039/140‑1 (MQ=255)

                                                                                  tcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTCTggg                                                     >  1:3216061/1‑140 (MQ=255)

                                                                                  tcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTCTggg                                                     >  1:3035963/1‑140 (MQ=255)

                                                                                   cccccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGa                                                    >  1:205247/1‑140 (MQ=255)

                                                                                     cccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATc                                                  <  2:1488566/140‑1 (MQ=255)

                                                                                          tttGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGa                                             <  1:6134249/140‑1 (MQ=255)

                                                                                            tgtgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa                                           <  1:621880/140‑1 (MQ=255)

                                                                                            tgtgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa                                           <  1:3931060/140‑1 (MQ=255)

                                                                                              tgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATTGGATCCAGGAAATg                                         >  2:5949564/1‑140 (MQ=255)

                                                                                               ggACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGa                                        <  2:3022291/140‑1 (MQ=255)

                                                                                                   tGCCCCCCCGGTCGACGATCCTGCCCTCTAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCACATCACTACACTTCAGGGGGGTATGAGTGTAGAGTTTCCCCATATGTGTCGATGGGATCCAGGACAGGACCAt                                    >  2:3642158/1‑140 (MQ=255)

                                                                                                   tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt                                    >  1:4339399/1‑140 (MQ=255)

                                                                                                    gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATc                                   >  1:1829640/1‑140 (MQ=255)

                                                                                                     cccccccGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                  >  2:2119409/1‑140 (MQ=255)

                                                                                                     cccccccGGTAGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                  >  2:1329626/1‑140 (MQ=255)

                                                                                                       cccccGCGAGACGATCCTGCCCGAGCGGTGGA‑GTGCC‑CCCT‑CTTCAGGTGGCTAACTTTAGCAAATCCATACACTTCAGGGGGGTAAGATTGTAGATTGTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAgg                                <  2:2987791/140‑1 (MQ=255)

                                                                                                           cGGTAGACGATCCTGCCCTCTAGTTGGA‑GGGCC‑CCCT‑CTTCAGGTGGCTAAATTTAGCAAATCCATACACTTCAGGGGGGTATTAGTGTAGAGTTTCCCCAGATGTGTCTCTGGGCTCCAGGACATGACAATCAGGAGtt                            >  2:2460584/1‑140 (MQ=255)

                                                                                                               aGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTATCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:1431809/140‑1 (MQ=255)

                                                                                                               aGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:738696/140‑1 (MQ=255)

                                                                                                               aGACGATCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:5925647/140‑1 (MQ=255)

                                                                                                               aGACGATCCTGCCCTAGAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  2:2000686/140‑1 (MQ=255)

                                                                                                                     tCCTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTCTGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACtt                  >  2:2422106/1‑140 (MQ=255)

                                                                                                                       cTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     >  1:4753546/1‑55 (MQ=255)

                                                                                                                       cTGCCCTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaa                >  2:6083482/1‑140 (MQ=255)

                                                                                                                          cccTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATc             >  1:4399766/1‑140 (MQ=255)

                                                                                                                          cccTATAGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATc             <  1:3972948/140‑1 (MQ=255)

                                                                                                                              ataGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTgtt         >  1:2916592/1‑138 (MQ=255)

                                                                                                                               taGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  1:262741/1‑140 (MQ=255)

                                                                                                                               taGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  2:3940336/140‑1 (MQ=255)

                                                                                                                               taGTTGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  1:2363608/140‑1 (MQ=255)

                                                                                                                               taGTTGGA‑GTGCC‑CCCT‑AGTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTGTCTATGGGCTCCAGGACCTGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:3504815/1‑140 (MQ=255)

                                                                                                                               taGTTGGA‑GTG‑C‑CCCTGATTCAGTTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  2:3409511/140‑1 (MQ=255)

                                                                                                                                   tGGA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAggg    <  1:3876875/140‑1 (MQ=255)

                                                                                                                                    ggA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:1031218/1‑140 (MQ=255)

                                                                                                                                    ggA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  2:2784983/1‑140 (MQ=255)

                                                                                                                                    ggA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:6014036/1‑140 (MQ=255)

                                                                                                                                    ggA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:4307806/1‑140 (MQ=255)

                                                                                                                                     gA‑GTGCC‑CCCT‑ATTCAGGTGTCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATGAGTGTAGAGTTTCCCCATATGGTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTACATCTGGTCAGGGCa  <  2:6439695/140‑1 (MQ=255)

                                                                                                                                     gA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  1:3723352/140‑1 (MQ=255)

                                                                                                                                     gA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  2:2537062/140‑1 (MQ=255)

                                                                                                                                     gA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  2:1288738/140‑1 (MQ=255)

                                                                                                                                     gA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  1:6276254/140‑1 (MQ=255)

                                                                                                                                     gA‑GTGCC‑CCCT‑ATTCAGGTGGCTAAATTGAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  1:3011755/140‑1 (MQ=255)

CTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGA‑GTG‑C‑CCCT‑ATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NZ_CP025268/2769711‑2769982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`