BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,858,752	+T	pseudogene (395/2078 nt)	flhA →	formate hydrogenlyase transcriptional activator FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,858,748	1	.	T	96.4%	1228.7 / 30.6	330	pseudogene (391/2078 nt)	flhA	formate hydrogenlyase transcriptional activator FlhA
Reads supporting (aligned to +/- strand): ref base . (9/3); new base T (166/152); total (175/155)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 361 total aligned reads displayed.

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑T‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCat‑a                                                                                                                                           <  2:202296/140‑1 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAGACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  2:1672540/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGTCAGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  1:1039395/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  1:4722524/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  1:3318966/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  2:3046583/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  1:4991404/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  2:3940198/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  1:911302/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATAGCCTGATGCCACTGGCGGCGGACGGGCAT‑A‑t‑t‑tt                                                                                                                                    >  1:3679822/1‑140 (MQ=255)

      caTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tttg                                                                                                                                  >  2:1563474/1‑138 (MQ=255)

        ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tttggt                                                                                                                                <  1:3237385/140‑5 (MQ=255)

        ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tttggt                                                                                                                                <  2:3689255/140‑5 (MQ=255)

        ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑t‑t‑tttggt                                                                                                                                >  2:792963/1‑136 (MQ=255)

           tGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGc                                                                                                                              >  1:6190686/1‑140 (MQ=255)

             tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCtg                                                                                                                            <  2:2622043/140‑1 (MQ=255)

                cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCCAAATTTGGTCACTATTGCCTGATGCCACAGGCGGCGGAAGGGCAG‑A‑TGT‑TTGGTGGCGGTGa                                                                                                                         >  2:2103116/1‑140 (MQ=255)

                      gATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTAt                                                                                                                   <  1:5965838/140‑1 (MQ=255)

                       aTACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTAtt                                                                                                                  >  2:5536051/1‑140 (MQ=255)

                             tGCATTGCAGTTANGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑T‑TNTTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:5646562/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCATATTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTag                                                                                                            >  2:2710448/1‑139 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:460522/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:3218585/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:3875210/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:196411/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCGGGACGCTGGTGGGCTATACCAAAAATTTGGTCACTGTTGCGTGATGCCAGTGGCGGCGGATGGGCGT‑G‑TGT‑TTGGTGGGTGTGAGGTTATTCGttgg                                                                                                            >  1:5876772/1‑138 (MQ=255)

                              gCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTACTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATg                                                                                                           <  1:5096334/140‑1 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGTCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:1914460/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:3889318/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  2:2408631/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAAATTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:278430/1‑140 (MQ=255)

                                 ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        <  2:5504551/140‑1 (MQ=255)

                                 ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        <  2:5126811/140‑1 (MQ=255)

                                  tGCAGTTACGAATAAATTTGTGAAACCTGGCCGAAGCTGGACGCAGGTGGGCTATACAAAAAATTTGGAAACTATTGCCTGATGCCACAGGCGTCGGAAGGGCAT‑A‑TAT‑TTGGATGCTGTGAATTAATTCTTTATGACGa                                                                                                       >  2:1778666/1‑140 (MQ=255)

                                  tGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGa                                                                                                       >  1:5049038/1‑140 (MQ=255)

                                   gctgTTACGAAGTAGTTTGTGAAACCTTGACTCAGCTGTAATTAGGTGGGATATTCGCTTTTTTTGGTAACTTTTTCCTTATGACTCTGGCGTCGGTAGGTCATAA‑T‑T‑TTTGTGTTTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:5349745/137‑1 (MQ=255)

                                   gCAGTTACGTAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:17438/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:3046583/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:5061732/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:3423140/140‑1 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                <  1:4045549/140‑1 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  1:608770/1‑140 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                <  1:6337049/140‑1 (MQ=255)

                                           gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                                                                                              <  1:1826245/140‑1 (MQ=255)

                                           gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                                                                                              <  2:4544567/140‑1 (MQ=255)

                                           gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                                                                                              <  1:5277456/140‑1 (MQ=255)

                                              gaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGTTGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAg                                                                                           >  1:2533771/1‑140 (MQ=255)

                                              gaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAg                                                                                           >  2:5454730/1‑140 (MQ=255)

                                               aaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGc                                                                                          >  2:2777966/1‑140 (MQ=255)

                                                         aaCCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGt                                                                                >  1:2091598/1‑140 (MQ=255)

                                                            cTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAATTGGACACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCa                                                                             <  2:1130152/140‑1 (MQ=255)

                                                              ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt                                                                           >  1:807653/1‑140 (MQ=255)

                                                                ccGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGTTGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg                                                                         >  1:2360424/1‑140 (MQ=255)

                                                                     gCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGc                                                                    >  1:2984834/1‑140 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  1:6118192/140‑1 (MQ=255)

                                                                        ggACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCaaa                                                                 <  2:4122627/140‑1 (MQ=255)

                                                                           cGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACAt                                                              >  2:5928599/1‑140 (MQ=255)

                                                                              aGGTGGGCTATACCCAAAATTTGGTCTCTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTNATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCNAACATTTa                                                           <  1:5711232/140‑1 (MQ=255)

                                                                              aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTa                                                           >  1:4487060/1‑140 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         >  2:2819928/1‑140 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         <  2:1855585/140‑1 (MQ=255)

                                                                                   ggCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAATAGAGTTCAATCGTCTGCAATCATTTACGCAg                                                      >  2:3386826/1‑140 (MQ=255)

                                                                                   ggCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAg                                                      >  2:52361/1‑140 (MQ=255)

                                                                                    gCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGa                                                     <  1:5333575/140‑1 (MQ=255)

                                                                                    gCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGa                                                     >  2:618581/1‑140 (MQ=255)

                                                                                     cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                    <  1:3325814/140‑1 (MQ=255)

                                                                                     cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                    >  1:1461457/1‑140 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  1:4360271/1‑140 (MQ=255)

                                                                                          cccAAAATTTGGTCAATATTGCCTGATGACACTGGCGGCGGTAGGGCAT‑ATT‑T‑TTGGTGGCTGTGACTTTATTCGTTATGTCGAGCGCCCCTGGAGCGAATTAGAGTTCAATCGTCTGCAAACATTTACGGAGTTCGttt                                               >  2:3120552/1‑140 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           >  2:1428082/1‑140 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGGTATGTCGATAGCCGGAGGAGCGAAAAAGAGTTCAATCGGCTGCAATCTTTTACGCAGATCGTTTCTgt                                           >  2:2530895/1‑140 (MQ=255)

                                                                                              aaaTTTGGTCACGATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGACTTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           <  1:1132959/140‑1 (MQ=255)

                                                                                                   tGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCa                                      <  2:295114/140‑1 (MQ=255)

                                                                                                    ggTCACTAGTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                     <  1:5179314/140‑1 (MQ=255)

                                                                                                     gTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAcc                                    >  1:3138503/1‑140 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGTAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:604311/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:637612/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:1637915/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:4548098/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:6380347/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:3213635/140‑1 (MQ=255)

                                                                                                           aTTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGACTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACaa                              <  2:6405326/140‑1 (MQ=255)

                                                                                                               ccTGATGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATcc                          >  2:598730/1‑140 (MQ=255)

                                                                                                                   aTGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCagag                      >  2:6130939/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:5313004/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:1402716/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:4893455/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:3973043/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:3281287/1‑140 (MQ=255)

                                                                                                                     gCCACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                    <  2:3350399/140‑1 (MQ=255)

                                                                                                                      ccACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAATAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcg                   >  2:6079026/1‑140 (MQ=255)

                                                                                                                      ccACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcg                   >  2:5815753/1‑140 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  1:4389142/140‑1 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  2:440273/140‑1 (MQ=255)

                                                                                                                        aCTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgcg                 <  1:4539955/140‑1 (MQ=255)

                                                                                                                         cTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgt                >  2:1274478/1‑140 (MQ=255)

                                                                                                                         cTGGCGGCGGAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgt                <  2:3570823/140‑1 (MQ=255)

                                                                                                                         cTGGCGGAGGAAAGGCATAA‑T‑A‑ATGGTTCCTGAGAATAAAATCCTAATGAAGATCGCCCCTGGAGCTAAAAAGACATCAATCCACTGAAAACATTTAAGCAGATCGAATCTGTCGTCACCGAACAAATCCAGAGCcgcca                >  2:4944694/1‑138 (MQ=255)

                                                                                                                                cggAAGGGCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAAc         >  1:694450/1‑140 (MQ=255)

                                                                                                                                     gggCAT‑ATT‑T‑TTGGTGGCTGTGAATTTATTCGTTCTGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGt    <  2:4237799/140‑1 (MQ=255)

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑T‑T‑TTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`