BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,014,858	+G	intergenic (+159/+61)	arcC → / ← yqeB	carbamate kinase/selenium‑dependent molybdenum cofactor biosynthesis protein YqeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,014,857	1	.	G	98.3%	1024.8 / 8.2	304	intergenic (+158/+62)	arcC/yqeB	carbamate kinase/selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
Reads supporting (aligned to +/- strand): ref base . (4/1); new base G (164/133); total (168/136)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 317 total aligned reads displayed.

ATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG  >  NZ_CP025268/3014719‑3014991

   ccATCCGGCCAAAGATTGTCAAGTAAGTGCGCTGTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CA‑ggca                                                                                                                                    >  2:5307570/1‑137 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAATTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCACATTCAATCGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAAtt                                                                                                                                  >  2:6024060/1‑140 (MQ=25)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAAtt                                                                                                                                  >  1:4201044/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAAtt                                                                                                                                  >  2:5048658/1‑140 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CA‑ggcaat                                                                                                                                  <  2:1489207/139‑6 (MQ=255)

      tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGACATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAAtt                                                                                                                                  >  2:212039/1‑140 (MQ=255)

      tCCGGCCAAAGATGGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACACAACATCGCAAATTCAATAGATTGCCATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CA‑ggccctt                                                                                                                                 >  2:5120180/1‑134 (MQ=25)

       ccGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAAttt                                                                                                                                 >  1:1542122/1‑140 (MQ=255)

        cGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTg                                                                                                                                >  2:1873052/1‑140 (MQ=255)

        cGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTg                                                                                                                                >  1:246260/1‑140 (MQ=255)

          gCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGc                                                                                                                              >  1:5518593/1‑140 (MQ=255)

          gCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGc                                                                                                                              >  2:1787776/1‑140 (MQ=255)

           ccAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCg                                                                                                                             >  2:685905/1‑140 (MQ=255)

                   tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGtcat                                                                                                                     <  2:4048422/140‑1 (MQ=255)

                   tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGtcat                                                                                                                     <  1:3662755/140‑1 (MQ=255)

                   tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGtcat                                                                                                                     <  2:3993634/140‑1 (MQ=255)

                   tGTCAAGTAAGTGCGCTTTGATGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGtcat                                                                                                                     <  2:5211199/140‑1 (MQ=255)

                    gTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGtcatc                                                                                                                    >  1:5844633/1‑140 (MQ=255)

                             gtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAgg                                                                                                           <  1:2594248/140‑1 (MQ=255)

                              tGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGc                                                                                                          <  1:5066275/140‑1 (MQ=255)

                               gcgcTTTGTTGATGCCGTATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGcc                                                                                                         >  1:2130354/1‑140 (MQ=255)

                               gcgcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGcc                                                                                                         >  1:6301664/1‑140 (MQ=255)

                               gcgcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGcc                                                                                                         >  1:3262243/1‑140 (MQ=255)

                               gcgcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGcc                                                                                                         >  1:1409091/1‑140 (MQ=255)

                                cgcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCg                                                                                                        <  1:685905/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:6285037/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:2491941/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:1257939/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:1325551/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:1482400/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       >  1:4434854/1‑140 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:4287106/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:3627131/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:3751130/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:4435162/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCGACAAAACATCGCATATTCAATAGATGGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:6336745/140‑1 (MQ=21)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTGATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:3347548/140‑1 (MQ=255)

                                 gcTTTGTTGATGCCGGATGCGGCGCGAGCGACTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:2445538/140‑1 (MQ=255)

                                   tttGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggt                                                                                                     <  1:6089215/140‑1 (MQ=255)

                                   ggtgatgATGCCGGATGCGGCGCGAGCGCCTTATCCGACCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAAATTGGCGTTTGTCATCAGTCTCAGGCCgggt                                                                                                     <  1:4066753/135‑1 (MQ=21)

                                          aTGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGtcatc                                                                                                                    >  2:5384843/1‑118 (MQ=255)

                                          aTGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAAt                                                                                              >  2:2377456/1‑140 (MQ=255)

                                          aTGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAAt                                                                                              >  1:2178207/1‑140 (MQ=255)

                                           tGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATa                                                                                             <  1:2758878/140‑1 (MQ=255)

                                               ggATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAACTTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCAt                                                                                         <  1:1176126/140‑1 (MQ=255)

                                               ggATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCAt                                                                                         >  2:264057/1‑140 (MQ=255)

                                                      gcgcGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCTATAGAGTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCa                                                                                  >  2:4630787/1‑140 (MQ=255)

                                                      gcgcGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCa                                                                                  >  1:6389106/1‑140 (MQ=255)

                                                        gcgAGCGCCTTATCCGTCCTACAAAACATCGCACATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAtt                                                                                <  1:4516946/140‑1 (MQ=255)

                                                        gcgAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGCGTAATACCATCCGGCCAtt                                                                                <  1:3241516/140‑1 (MQ=255)

                                                         cgAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCAttt                                                                               <  1:887283/140‑1 (MQ=255)

                                                                       gTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGtt                                                                                                                           <  1:3531596/82‑1 (MQ=255)

                                                                        tCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACtt                                                                >  1:2945857/1‑140 (MQ=255)

                                                                            aCAAAACATCGCAAATTCAATAGATTGCAATGCACTGGTAGGCCTGATAAGCGGAGCG‑CATCCG‑GCAAGTTGGCGTTTGTCAGCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTGCCAg                                                            >  2:4281129/1‑140 (MQ=37)

                                                                                aaCATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCAct                                                        <  1:1989057/140‑1 (MQ=255)

                                                                                  cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCActtc                                                      >  1:2010035/1‑140 (MQ=255)

                                                                                     cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCttt                                                   >  1:666827/1‑140 (MQ=255)

                                                                                          aTTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCg                                              <  1:5282160/140‑1 (MQ=255)

                                                                                            tCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCt                                            >  1:4391257/1‑140 (MQ=255)

                                                                                             cAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCtt                                           <  2:497573/140‑1 (MQ=255)

                                                                                              aaTAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCttt                                          >  1:5601251/1‑140 (MQ=255)

                                                                                                tAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa                                        <  2:6060586/140‑1 (MQ=255)

                                                                                                  gATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACg                                      <  2:3896848/140‑1 (MQ=255)

                                                                                                  gATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACg                                      <  1:3924561/140‑1 (MQ=255)

                                                                                                    ttGCAAGGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGcc                                    <  2:1122936/140‑1 (MQ=255)

                                                                                                     tGCAATGCACTTGTAGGCCTGATAATCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  1:3436601/1‑140 (MQ=255)

                                                                                                     tGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  1:1751264/1‑140 (MQ=255)

                                                                                                     tGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTGTCACGccc                                   >  1:1056702/1‑140 (MQ=255)

                                                                                                     tGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACAATCCGGCCATTTTCATTTAAGCCACTTCCAGCGCTTCTTTTGTCGCTTTCACGccc                                   >  2:645662/1‑140 (MQ=255)

                                                                                                      gCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCt                                  <  1:4951671/140‑1 (MQ=255)

                                                                                                        aaTGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGa                                <  2:1239332/140‑1 (MQ=255)

                                                                                                          tGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATg                              >  1:394171/1‑140 (MQ=255)

                                                                                                          tGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATg                              >  1:5402053/1‑140 (MQ=255)

                                                                                                             aCTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCACTTTGGCGTTTGTCATCAGTCTCCGGCCGGGTGGGTAATACCATCCGGCCATTTTCAGTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGcct                           >  2:1629067/1‑138 (MQ=37)

                                                                                                             aCTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTGCGTTTGTCGCTTTCACGCCCTGATGCAt                           >  2:899278/1‑140 (MQ=255)

                                                                                                              cTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATc                          <  1:6050986/140‑1 (MQ=255)

                                                                                                                tGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCaa                        <  2:384889/140‑1 (MQ=255)

                                                                                                                tGTAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCACAGGCCGGGTGGGTAATACCATCCGGCCAATTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCaa                        <  2:3538853/140‑1 (MQ=255)

                                                                                                                 gttttccTGTTTAGCGTAGCGAAAC‑CG‑GCAATTTGGCGTTTGTAATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAc                       <  2:4768310/135‑1 (MQ=255)

                                                                                                                 gTAGGCCTGATAAGCGTAGCT‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAc                       <  1:3565018/140‑1 (MQ=255)

                                                                                                                  tAGGCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCAGCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAca                      >  1:5993897/1‑140 (MQ=255)

                                                                                                                    ggCCTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCAATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatc                    >  2:3064727/1‑140 (MQ=255)

                                                                                                                      ccTGATAAGCGTAGCG‑CAT‑CAGGCACTTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCNCTTTCACGCCCTGATGCATCAAcatcat                  >  1:5671505/1‑140 (MQ=255)

                                                                                                                      ccTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACGATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatcat                  >  2:2424322/1‑140 (MQ=255)

                                                                                                                      ccTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatcat                  >  1:6361925/1‑140 (MQ=255)

                                                                                                                      ccTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatcat                  >  1:2352261/1‑140 (MQ=255)

                                                                                                                       cTGATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCTTTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCAtt                 <  1:2905774/140‑1 (MQ=255)

                                                                                                                         gATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  1:2312286/1‑140 (MQ=255)

                                                                                                                         gATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  2:6155938/1‑140 (MQ=255)

                                                                                                                         gATAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTGTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  2:3161791/1‑140 (MQ=255)

                                                                                                                          aTAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAAc              <  1:2699717/140‑1 (MQ=255)

                                                                                                                          aTAAGCGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAAc              >  1:952453/1‑140 (MQ=255)

                                                                                                                               cGTAGCG‑CAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGcc                                    <  1:2396726/113‑1 (MQ=255)

                                                                                                                               cGTAGCG‑CAT‑CAGGCAATTTGGAGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCAACTTCCAGCACTTCTTTTGTCGATTTCACGCCCTGATGCATCAAAATCATTAACGCCTc         >  2:5617159/1‑140 (MQ=39)

                                                                                                                                   gcg‑cAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGt     <  2:2834338/140‑1 (MQ=255)

                                                                                                                                    cg‑cAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa    >  1:3370269/1‑140 (MQ=255)

                                                                                                                                    cg‑cAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa    >  1:4634559/1‑140 (MQ=255)

                                                                                                                                    cg‑cAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa    >  1:5729474/1‑140 (MQ=255)

                                                                                                                                    cg‑cAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa    >  2:3494288/1‑140 (MQ=255)

                                                                                                                                    cg‑cAT‑CAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATAAACGCCTCAAGTa    >  2:1702379/1‑140 (MQ=255)

ATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCG‑CAT‑CA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG  >  NZ_CP025268/3014719‑3014991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`