Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5530TA25.8% 324.8 / 27.2 168intergenic (+84/+68)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (23/95);  new base A (41/0);  total (72/96)
Fisher's exact test for biased strand distribution p-value = 7.80e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 170 total aligned reads displayed.

ACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCG  >  NZ_CP025268/3505415‑3505692
                                                                                                                                          |                                                                                                                                            
aCTCTTCTGCCGCGCCAATCGCATCACCTTATCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTa                                                                                                                                             <  2:690291/140‑1 (MQ=255)
  tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAc                                                                                                                                           <  1:2364369/140‑1 (MQ=255)
   cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                          <  2:3142546/140‑1 (MQ=255)
   cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                          <  2:1243836/140‑1 (MQ=255)
   cgtctGCCGCGCCAAGCGCATCACCTTAACTATTGAGAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                          <  2:2392287/138‑1 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAccc                                                                                                                                         >  1:2717114/1‑140 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAAccc                                                                                                                                         >  1:2801798/1‑140 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAacc                                                                                                                                         >  1:4143952/1‑140 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  1:4850809/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  2:1664381/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  1:2775375/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCTAACCct                                                                                                                                        >  1:2331215/1‑140 (MQ=255)
         ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTCCGGTCCCCTCACACTAACCCACT‑cc                                                                                                                                   >  2:3071690/1‑140 (MQ=39)
            cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCa                                                                                                                                <  1:3000909/140‑1 (MQ=255)
            cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACAACCAAAACCACA‑ccccc                                                                                                                                >  1:2176123/1‑139 (MQ=38)
                aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAgg                                                                                                                            <  1:5729713/140‑1 (MQ=255)
                aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAgg                                                                                                                            <  2:5965619/140‑1 (MQ=255)
                aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTAGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAgg                                                                                                                            <  2:4455741/140‑1 (MQ=255)
                  tCGCATCACCTTACCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAgggg                                                                                                                          <  1:6159484/140‑1 (MQ=255)
                     cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGa                                                                                                                       <  2:1430062/140‑1 (MQ=255)
                          ccTTAACTCTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACACTCT‑CCCCAAAGGGGCGAGGGGa                                                                                                                  <  1:5050263/140‑1 (MQ=255)
                          ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGa                                                                                                                  <  1:3940208/140‑1 (MQ=255)
                              aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAAACCAATACCCCAAAGGGG‑GAGGGGGCCGt                                                                                                              >  1:4059662/1‑140 (MQ=37)
                               aCTATTGATAATAACAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCACAGGGGCGAGGGGACCGtt                                                                                                             <  1:1455821/140‑1 (MQ=255)
                               aCTATTGATAATAAAAGACAGTAACCGTAGGCCGGATAAGAGGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGtt                                                                                                             <  1:2065356/140‑1 (MQ=255)
                                  aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCGAACCCACT‑ACCCCAAGGGGCGAGGGGACCGGTCat                                                                                                          >  1:1627953/1‑139 (MQ=38)
                                   ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCAAA‑CCCCAAAGGGGCCAGGGGACCGTTCAGt                                                                                                         >  2:1342647/1‑140 (MQ=38)
                                   ttGATAATAAAAGACATTAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGAAGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCAAACCCAAACCCAAA‑ACCCAAAGGGGCGAGGGGACCGGTAAGt                                                                                                         >  2:2706617/1‑140 (MQ=37)
                                    tGATAATAAAAGACATTAACNGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCCCACCCAAANCCACT‑CCNCAAAGGGGCGGGGGGGCCCGTCAGTa                                                                                                        >  1:5630117/1‑140 (MQ=37)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCCCCCGAACCCACA‑CCCCAAAGGGGCGCGGGGGCCgggcagga                                                                                                        >  1:641164/1‑133 (MQ=37)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                        >  2:6211030/1‑140 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACA‑CCCCAAAGGGGCGAGGGGACCGGTCAGTa                                                                                                        >  2:2363184/1‑140 (MQ=39)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAAACCACT‑CACCAAAGGGGCGAGGGGACCGTTGAGTa                                                                                                        >  2:5063081/1‑140 (MQ=38)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAAACCACA‑CACCAAAGGGGCGCGGGGACCGGTCAGTa                                                                                                        >  2:4170290/1‑140 (MQ=37)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCAAA‑ACCCCAACGGGCGAGGGGGCCGGTAAGTAc                                                                                                       >  1:5156638/1‑140 (MQ=37)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACA‑ACCCAAAAGGGGGAGGGGGCCGGTCAggtc                                                                                                       >  1:5143766/1‑137 (MQ=37)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACT‑ACCCAAAGGGGCGAGGGGACCCGTAAGTAc                                                                                                       >  1:3608961/1‑140 (MQ=38)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                       >  2:3683172/1‑140 (MQ=255)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTAACCCTAACCCACT‑CCCCCAATGGGCGAGGGGACCGGTCAGTaa                                                                                                       >  1:822115/1‑139 (MQ=38)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTAACCTCACCCTAACCCACT‑CCCCAAAGGGGCGAGGGGACCGTTCAGtta                                                                                                       >  2:3925527/1‑138 (MQ=255)
                                             aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACACACACCCTAACCCACA‑AACCAAAAGGGCGATGGGACCGGTCAGTAAGGAAATTa                                                                                               >  2:3010209/1‑140 (MQ=37)
                                                aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                            <  2:1046073/140‑1 (MQ=255)
                                                aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCCAAACCACA‑ACCCAAAAGGGCGTGGGGGCCCGTCAGTAAGGCATTTAggt                                                                                            >  1:2253940/1‑138 (MQ=25)
                                                 cATTAACCGTAGGCCTGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATAGTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCtt                                                                                           <  2:4327575/140‑1 (MQ=255)
                                                  aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCTGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAACCAAAACCTAT‑ACCCAAAGGGGCGAGGGGACCGGTCAGTACCGAAATTATCTTg                                                                                          >  2:4194528/1‑140 (MQ=37)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTGACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                         <  2:6127083/140‑1 (MQ=255)
                                                    tAACCGTATGCCGGATAAGATGCGCCAGCATCTCATCCGGCGATGCTGTCGCGTAGAATGTTTCCTACCGGACGTTCACCACACCCTAACCGGCT‑CCGCCACGTGGCGTGGGGAACGTTCAGTTCGGAATAGAGGTTGAc                                                                                        >  2:3538447/1‑140 (MQ=16)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTCACCCTCT‑CCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                    <  2:11238/140‑1 (MQ=255)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                    <  2:1987580/140‑1 (MQ=255)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                    <  2:1819867/140‑1 (MQ=255)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                    <  1:3500239/140‑1 (MQ=255)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGGTCCCCTCACCCTAACCCTCT‑CCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                    <  2:4684956/140‑1 (MQ=255)
                                                          tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCACT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCt                                                                                  >  2:4622154/1‑140 (MQ=255)
                                                          tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACCCCCAAAACCCAA‑ACCCAAATGGGCGAGGGGACCGTTCCGTACCGAATTTAGGGTGACGGGGCt                                                                                  >  1:4327006/1‑140 (MQ=25)
                                                          tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACT‑ACCCCAAGGGGCGAGGGGACCGGTAAGTACGGAAATTAGCATGACGGTGCt                                                                                  >  1:6267611/1‑140 (MQ=37)
                                                           aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGa                                                                                                                  >  2:5912594/1‑107 (MQ=255)
                                                           aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGa                                                                                                                  <  1:5912595/107‑1 (MQ=255)
                                                            ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGCCCGTTCCGTACGGAAATTTGCTTGTCGGTGCTAt                                                                                >  1:4583593/1‑140 (MQ=38)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                               <  2:4148256/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                               <  2:6256948/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                               <  1:135487/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                               <  1:2725576/140‑1 (MQ=255)
                                                               cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGACTTTTACATCCCGTACGTTCCCCTCACACTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                             <  2:410906/140‑1 (MQ=255)
                                                               cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                             <  2:5705576/140‑1 (MQ=255)
                                                               cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                             <  1:5517624/140‑1 (MQ=255)
                                                                 gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCAAACCCACT‑CCCCAAAGGGGGGAGGGGCCCCTGAAGGAACGCATTTATCTTGTGGGTGAGATCCAGc                                                                           >  2:4760341/1‑140 (MQ=255)
                                                                 gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCGAACCCACT‑ACCCAAATGGGCGCGGGGACCGTTCAGTACGGAATTTAGCTTGGCCGTGCTATCCAGc                                                                           >  2:379010/1‑140 (MQ=255)
                                                                 gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCAAAACCGCT‑ACCCAAAGGGGCGGGGGGCCCGTTCAGGACGGAATTTTGGTGGGCGGTGCGATCCCGc                                                                           >  1:647162/1‑140 (MQ=255)
                                                                  aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAACCAAAACCACA‑ACCCAAAAGCGCGAGGGGACCGTTACGTACGGAATTTATCAAGACGGTGATAACCAACa                                                                          >  2:2633873/1‑140 (MQ=255)
                                                                    aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                        <  1:4761430/140‑1 (MQ=255)
                                                                      gATGCGCCAGCATCGCTTCCGGCGATGCTGGCGCTTTGAATTTTTCATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTAAGTAATTTAGCTTGACGGTGCTATCCAGCTTTaa                                                                      <  2:2203280/140‑1 (MQ=38)
                                                                        tGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                    <  2:191947/140‑1 (MQ=255)
                                                                         gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                   <  1:3286018/140‑1 (MQ=255)
                                                                          cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                  <  1:3958007/140‑1 (MQ=255)
                                                                           gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAAACCACT‑ACCCAAAGGGGCGAGGGGACCGTTGAGGACGGAATTTATGTTGTCGGTTCTATCCAGCATTAAATACt                                                                 >  1:4479450/1‑140 (MQ=255)
                                                                             cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                               <  1:3660740/140‑1 (MQ=255)
                                                                                  tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                          <  1:1922247/140‑1 (MQ=255)
                                                                                      atcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActct                                                      <  1:4197374/140‑1 (MQ=255)
                                                                                       tgcGGCGATGCTGGCGCGTTGAGTTTTACTTCCCGGACGTTCCCCTCCCCCGGTGCCTCT‑CCCGCGCGGGGGGGGGGGACCGGGCAGGCCGGAATTTAGCTGGGCGGTGCGAGCCCGGGTTTAATAGTGGGAATActccc                                                     >  2:4542182/3‑138 (MQ=255)
                                                                                       tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCAAACCCACA‑CCCCAAAGGGGCGAGGGGCCCGGTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATACTGGCAATACtctc                                                     >  2:5428751/1‑140 (MQ=255)
                                                                                           gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACT‑CCCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAAATTAGCATTAAGGTTGAATCCAGCATTAAATACTTGCAATACACTCACGc                                                 >  2:4941878/1‑140 (MQ=255)
                                                                                              aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                              <  2:2139701/140‑1 (MQ=255)
                                                                                              aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                              <  1:5228190/140‑1 (MQ=255)
                                                                                               tGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCACT‑CCCCCAAGGGGCGGGGGGACCGTTCAGTACGGAATTTTGCGTGACGGGGGGATCCAGCCTTAAATAAAGGCAAAACTCACAAGCaaaa                                             >  1:6380819/1‑140 (MQ=255)
                                                                                                gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCT‑CCCCAAAGGGGCGCGGGGACCGTTCAGTACGGCAGTTATCGTGACGGTGCTATCCAGCAATAAATACTGGCAATACTCTCACGCaaaaa                                            >  2:4877317/1‑139 (MQ=255)
                                                                                                gCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAAt                                            <  1:2586219/140‑1 (MQ=255)
                                                                                                  tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCACT‑CCCCAAAGGGGCGGGGGGACCGGTCAGGACGGAAATTAGCTTGTCGGTGCTATCCAGCCATAAATACAGGCAATAATCTCACGCCAAATcc                                          >  1:6361505/1‑140 (MQ=255)
                                                                                                  tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAAACCACT‑CCCCAAAGGGGCGGGGGGACCGGTCAGTACGGCATTTAGCATGACGGTGCTATCCAGCCATAAAAACAGGCAAAACTCAAACCCaaaaaac                                          >  1:1619215/1‑137 (MQ=255)
                                                                                                       cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                     <  1:132760/140‑1 (MQ=255)
                                                                                                         ttGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCG‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                   <  1:230819/140‑1 (MQ=255)
                                                                                                         ttGAATTTTACATCCCGTACGTTACCCTAACCCAAACCCACT‑CCCCAAAGGGGCGAGGGGACCGGTCAATAAGGAAATTAGCTTGACCGTGCAAACCAGCATTAAATACTGGCAAAACACTCACGCCAAATCCgaagcaa                                   >  2:863339/1‑139 (MQ=255)
                                                                                                              ttttACATCCCGTACGTTCCCCTCCCCCTTACCCTCT‑ACCCTAAGGGGCGAGGGGACCGGTCCGTACGGAATTTAGCTTGACCGTGCTATCCAGCAGTAACTACTGGCAAGACACTCACGCAAAATCCGCAGCACCGtcg                              >  2:6000071/1‑139 (MQ=255)
                                                                                                              ttttACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCACAGGGGGGAGGGGTCCGTTCAGTACGGAATTTAGCGTGACGGTGCTATCCACCATTAAATAATGGCAATACTCACACGCAAAATCCGCAGCACCGtct                              >  2:3441237/1‑140 (MQ=38)
                                                                                                                        cGTACGTTCCCCTCACCCAAACCCTCA‑CCCCAAAGGGGCGCGGGGACCGGTCAGTAACGAATTTATCTTTACGGGGCTATACAACAATAAATAATGGCAATACTCTCACGCAAAATACGCAGCACCGACACTTAACCGcg                    >  2:138502/1‑139 (MQ=21)
                                                                                                                              ttCCCCTCCCCCTAACCCTCG‑CCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt              <  2:1452723/140‑1 (MQ=255)
                                                                                                                              ttCCCCTCACCCTAACCCTCT‑CCCCTAAGGGGCGATGGGACCTTTCAGTACGGACTTTCGCTGGACGGTGCTATCCAGCATTAACTACTGTCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCCCCGCTGCGAtt              <  2:2964262/140‑1 (MQ=38)
                                                                                                                                  ccTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAATAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg          >  2:5769194/1‑140 (MQ=255)
                                                                                                                                    gcACCCGAACCCAAA‑CACCAAAGGGGCGAGGGGACCCTTAAGTAAGGAATTTAACTTTACGGTGCTATACAACAATAAATAATGGCAATACACACAACCAAAATACGCAACAACCTATCTTAAACCCAGCGATTAGGGtt        >  2:506854/2‑140 (MQ=255)
                                                                                                                                       cccTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt     <  2:2801798/140‑1 (MQ=255)
                                                                                                                                          tAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCg  <  1:5461709/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
ACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCG  >  NZ_CP025268/3505415‑3505692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑