Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5590TA26.6% 314.2 / 33.3 163intergenic (+90/+62)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (28/88);  new base A (42/0);  total (73/90)
Fisher's exact test for biased strand distribution p-value = 7.19e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 173 total aligned reads displayed.

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698
                                                                                                                                           |                                                                                                                                            
tctGCCGCGCCAATCGCATCACCTTATCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                              <  2:3088469/140‑1 (MQ=255)
tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCTAACCct                                                                                                                                              >  1:2331215/1‑140 (MQ=255)
       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCa                                                                                                                                      <  1:3001040/140‑1 (MQ=255)
       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACAACCAAAACCACA‑ccccc                                                                                                                                      >  1:2176123/1‑139 (MQ=38)
       cgcCAATCGCATCACCTTAACTAGTGATAATAAAAGACATTCACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCGCACCCTCT‑CCCCa                                                                                                                                      <  2:1493510/140‑1 (MQ=39)
         ccACTCGCCTCACCTTCCCTATGCCTACCAAAAGCCCTTACCCGTAGGCCCGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTAGAAATTGACATCCCGTACGTTCCCCGCACCCTAACCCTCT‑CCCCaac                                                                                                                                    <  2:3023928/140‑2 (MQ=21)
         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCaaa                                                                                                                                    <  2:6105146/140‑1 (MQ=255)
           aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAgg                                                                                                                                  <  1:5729713/140‑1 (MQ=255)
           aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAgg                                                                                                                                  <  2:5965619/140‑1 (MQ=255)
           aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTAGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAgg                                                                                                                                  <  2:4455741/140‑1 (MQ=255)
                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGa                                                                                                                             <  2:4938042/140‑1 (MQ=255)
                     ccTTAACTCTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACACTCT‑CCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:5050263/140‑1 (MQ=255)
                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:3940208/140‑1 (MQ=255)
                      cTTAACTATTGATAATAAAAGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCT‑CCCCCAAGGGGCGAGGGGAc                                                                                                                       <  2:586306/140‑1 (MQ=255)
                         aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAAACCAATACCCCAAAGGGG‑GAGGGGGCCGt                                                                                                                    >  1:4059662/1‑140 (MQ=37)
                          aCTATTGTTAATAAACGACATTAACCGTAGGCCGGATATGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGTATTTTACATCCCGTACGTGCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACGGtt                                                                                                                   <  2:2013963/140‑1 (MQ=38)
                          aCTATTGATAATAACAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCACAGGGGCGAGGGGACCGtt                                                                                                                   <  1:1455821/140‑1 (MQ=255)
                          aCTATTGATAATAAAAGACAGTAACCGTAGGCCGGATAAGAGGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  1:2065356/140‑1 (MQ=255)
                          aCTAGTGCTAATAACAGACATTACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCGGGCGCGTTGAATTTTCCATCCCGTACGTTCCCCTCCCCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  2:6196594/140‑1 (MQ=38)
                             aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCGAACCCACT‑ACCCCAAGGGGCGAGGGGACCGGTCat                                                                                                                >  1:1627953/1‑139 (MQ=38)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              >  2:6211030/1‑140 (MQ=255)
                               tGATAATAAAAGACATTAACAGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAACCTAACCCACT‑ACCCAAACGGGCGAGGGGACCGTTCAGTa                                                                                                              >  2:3459908/1‑140 (MQ=39)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGAACCCTCA‑ACCCAAAGGGGCGAGGGGACCCTTCAGTAc                                                                                                             >  1:6384088/1‑140 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACA‑ACCCAAAAGGGGGAGGGGGCCGGTCAggtc                                                                                                             >  1:5143766/1‑137 (MQ=37)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCAAACCCTCT‑CCCCCACGGGGCGCGGGGACCGTTCAGTAc                                                                                                             >  2:4827382/1‑140 (MQ=39)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACT‑ACCCAAAGGGGCGAGGGGACCCGTAAGTAc                                                                                                             >  1:3608961/1‑140 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTAACCCTAACCCACT‑CCCCCAATGGGCGAGGGGACCGGTCAGTaa                                                                                                             >  1:822115/1‑139 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAAACCACT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                             >  1:5568125/1‑140 (MQ=39)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAACCCTCT‑ACCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                             >  2:1420294/1‑140 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTAACCTCACCCTAACCCACT‑CCCCAAAGGGGCGAGGGGACCGTTCAGtta                                                                                                             >  2:3925527/1‑138 (MQ=255)
                                        aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCACT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTAAGGAATTTa                                                                                                     >  2:2726273/1‑140 (MQ=255)
                                           aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                                  <  2:1075790/140‑1 (MQ=255)
                                           aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                                  <  2:1046073/140‑1 (MQ=255)
                                           aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCCAAACCACA‑ACCCAAAAGGGCGTGGGGGCCCGTCAGTAAGGCATTTAggt                                                                                                  >  1:2253940/1‑138 (MQ=25)
                                            cATTAACCGTAGGCCTGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATAGTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCtt                                                                                                 <  2:4327575/140‑1 (MQ=255)
                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCTGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAACCAAAACCTAT‑ACCCAAAGGGGCGAGGGGACCGGTCAGTACCGAAATTATCTTg                                                                                                >  2:4194528/1‑140 (MQ=37)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:4184778/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:4552161/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:4962777/140‑1 (MQ=255)
                                               tAACCGTATGCCGGATAAGATGCGCCAGCATCTCATCCGGCGATGCTGTCGCGTAGAATGTTTCCTACCGGACGTTCACCACACCCTAACCGGCT‑CCGCCACGTGGCGTGGGGAACGTTCAGTTCGGAATAGAGGTTGAc                                                                                              >  2:3538447/1‑140 (MQ=16)
                                               tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                              >  2:6315585/1‑140 (MQ=255)
                                                aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                             <  1:6315586/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTCACCCTCT‑CCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:11238/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:1987580/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:3500239/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGGTCCCCTCACCCTAACCCTCT‑CCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:4684956/140‑1 (MQ=255)
                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCACT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCt                                                                                        >  2:4622154/1‑140 (MQ=255)
                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACCCCCAAAACCCAA‑ACCCAAATGGGCGAGGGGACCGTTCCGTACCGAATTTAGGGTGACGGGGCt                                                                                        >  1:4327006/1‑140 (MQ=25)
                                                      aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:5912595/107‑1 (MQ=255)
                                                      aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGa                                                                                                                        >  2:5912594/1‑107 (MQ=255)
                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGCCCGTTCCGTACGGAAATTTGCTTGTCGGTGCTAt                                                                                      >  1:4583593/1‑140 (MQ=38)
                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  2:4148256/140‑1 (MQ=255)
                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  1:2725576/140‑1 (MQ=255)
                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  1:135487/140‑1 (MQ=255)
                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGACTTTTACATCCCGTACGTTCCCCTCACACTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  2:410906/140‑1 (MQ=255)
                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  2:5705576/140‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCCTCCGGCGCTGCAGCCGCGTGGCCTGGTCCCACCCGTACGTTCCCCTCACCCTAACCCTCC‑CCCCCCCGGGGCGAGGGGACCGTTCAGTACGGAATGGCGCTTGCCGGTGCTATCCAGCa                                                                                <  2:5064595/140‑1 (MQ=19)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                                <  2:2389480/140‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAACCAAAACCACA‑ACCCAAAAGCGCGAGGGGACCGTTACGTACGGAATTTATCAAGACGGTGATAACCAACa                                                                                >  2:2633873/1‑140 (MQ=255)
                                                               aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                              <  1:4761430/140‑1 (MQ=255)
                                                                 gATGCGCCAGCATCGCTTCCGGCGATGCTGGCGCTTTGAATTTTTCATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTAAGTAATTTAGCTTGACGGTGCTATCCAGCTTTaa                                                                            <  2:2203280/140‑1 (MQ=38)
                                                                 gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCT‑CCCCAAAGGGGCGAGGGGGCCGGTCAGTTCGGTATTTTGCGTGTCGGTGGTATCCAGCATTaa                                                                            >  1:6427406/1‑140 (MQ=38)
                                                                   tGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                          <  2:191947/140‑1 (MQ=255)
                                                                    gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                         <  1:3286018/140‑1 (MQ=255)
                                                                    gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACT‑CCCCAAAGGGGGGAGGGGCCCGGTCAGTACGGAATTTAGGTTGTAGGTGCTATCCAGCATtatata                                                                         >  1:6234079/1‑140 (MQ=255)
                                                                     cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                        <  1:3958007/140‑1 (MQ=255)
                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                       <  1:2766920/140‑1 (MQ=255)
                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAAACCACT‑ACCCAAAGGGGCGAGGGGACCGTTGAGGACGGAATTTATGTTGTCGGTTCTATCCAGCATTAAATACt                                                                       >  1:4479450/1‑140 (MQ=255)
                                                                        cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                     <  1:3660740/140‑1 (MQ=255)
                                                                             tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                                <  1:1922247/140‑1 (MQ=255)
                                                                                 atcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActct                                                            <  1:5724177/140‑1 (MQ=255)
                                                                                  tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCAAACCCACA‑CCCCAAAGGGGCGAGGGGCCCGGTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATACTGGCAATACtctc                                                           >  2:5428751/1‑140 (MQ=255)
                                                                                      gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACT‑CCCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAAATTAGCATTAAGGTTGAATCCAGCATTAAATACTTGCAATACACTCACGc                                                       >  2:4941878/1‑140 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  1:5228190/140‑1 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  2:2905889/140‑1 (MQ=255)
                                                                                           gCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAAt                                                  <  1:2586219/140‑1 (MQ=255)
                                                                                             tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCACT‑CCCCAAAGGGGCGGGGGGACCGGTCAGGACGGAAATTAGCTTGTCGGTGCTATCCAGCCATAAATACAGGCAATAATCTCACGCCAAATcc                                                >  1:6361505/1‑140 (MQ=255)
                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCG‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  1:230819/140‑1 (MQ=255)
                                                                                                    ttGAATTTTACATCCCGTACGTTACCCTAACCCAAACCCACT‑CCCCAAAGGGGCGAGGGGACCGGTCAATAAGGAAATTAGCTTGACCGTGCAAACCAGCATTAAATACTGGCAAAACACTCACGCCAAATCCgaagcaa                                         >  2:863339/1‑139 (MQ=255)
                                                                                                     tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                        <  1:2798949/140‑1 (MQ=255)
                                                                                                          tttACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGACTTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtctc                                   <  1:1410071/140‑1 (MQ=255)
                                                                                                               aTCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                              <  2:13489/140‑1 (MQ=255)
                                                                                                                 cccGTACGTTCCCCTCACCCTAACCCACT‑CCCCAAAGGGGCGAGGGGCCCGTTCAGTACGGAATTTATCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACAAACACGCAAAATCCGCAGCACCGTCTCTTCACCg                            >  2:3729182/1‑140 (MQ=39)
                                                                                                                         ttCCCCTCCCCCTAACCCTCG‑CCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                    <  2:1452723/140‑1 (MQ=255)
                                                                                                                         ttCCCCTCACCCTAACCCTCT‑CCCCTAAGGGGCGATGGGACCTTTCAGTACGGACTTTCGCTGGACGGTGCTATCCAGCATTAACTACTGTCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCCCCGCTGCGAtt                    <  2:2964262/140‑1 (MQ=38)
                                                                                                                         ttCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                    <  2:1806902/140‑1 (MQ=255)
                                                                                                                                  cccTCACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt           <  1:4183979/140‑1 (MQ=255)
                                                                                                                                     tAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCg        <  1:5461709/140‑1 (MQ=255)
                                                                                                                                      aaCCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:1033168/1‑140 (MQ=255)
                                                                                                                                      aaCCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  2:4114376/1‑140 (MQ=255)
                                                                                                                                      aaCCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       <  1:115291/140‑1 (MQ=255)
                                                                                                                                      aaCCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:2488062/1‑140 (MQ=255)
                                                                                                                                       aCCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      <  1:2832736/140‑1 (MQ=255)
                                                                                                                                        cccTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt     >  1:3145109/1‑140 (MQ=255)
                                                                                                                                         ccTCT‑CCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc    <  2:5143767/140‑1 (MQ=255)
                                                                                                                                         ccTCT‑CCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAGTCGGGTTAATGCGAATc    <  1:826755/140‑1 (MQ=255)
                                                                                                                                         ccTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc    <  2:5143766/140‑1 (MQ=255)
                                                                                                                                          ctct‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCg   >  1:5012037/1‑140 (MQ=255)
                                                                                                                                           tct‑cCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:4383294/1‑140 (MQ=255)
                                                                                                                                           tct‑cCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  2:2433198/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCT‑CCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑