Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268922,3060AT21.7% 351.9 / 25.3 165intergenic (‑342/+153)lysO/aqpZL‑lysine exporter LysO/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base A (105/14);  new base T (1/32);  total (109/56)
Fisher's exact test for biased strand distribution p-value = 9.42e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 165 total aligned reads displayed.

GGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAG  >  NZ_CP025268/922173‑922444
                                                                                                                                      |                                                                                                                                           
ggggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGggc                                                                                                                                       >  2:4902399/1‑140 (MQ=32)
  ggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGTGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGggcgg                                                                                                                                     <  2:323007/140‑1 (MQ=17)
  ggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGggcgg                                                                                                                                     >  2:1575670/1‑140 (MQ=32)
  ggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGggcgg                                                                                                                                     >  1:555536/1‑140 (MQ=32)
  ggCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGggcgg                                                                                                                                     <  1:3018915/140‑1 (MQ=32)
       cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGgcggcggcg                                                                                                                                <  2:2797640/140‑1 (MQ=32)
            aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTgg                                                                                                                           >  1:5570401/1‑140 (MQ=32)
             tgtttgtgGGCTGAGGGCGCTTTTTACAGGGATGTTACCTCGCGCCCGACCCGTTAGCCGTAAGGGATACGTGGTGGGCACCGCGCAGCGGCTATTTTGTT‑CGCCAGAGCCCGGGGGTGCTGGGGGGGGGGGCGTTTGg‑c                                                                                                                         <  1:2878697/136‑1 (MQ=11)
             tGGATGTGGGCTGCGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGCGCCCGGGGGTGCAGGGGGCGGCGGCGATTGg‑c                                                                                                                         <  1:121604/140‑1 (MQ=17)
             tGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGg‑c                                                                                                                         <  2:1042839/140‑1 (MQ=32)
              gtaTGTGGGCTGTGGGCGCGTTTTACTGGGATGTTACCTCGCGCCCGACCGGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGTTTTTGTT‑CGCCAGAGCCGGGGGGTGCTGGGGGCGGCGGCGTTTGg‑cc                                                                                                                        <  2:969020/138‑1 (MQ=11)
                 tgtgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGg‑ccgcc                                                                                                                     >  2:5246881/1‑140 (MQ=32)
                 tgtgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGg‑ccgcc                                                                                                                     >  1:3462765/1‑140 (MQ=32)
                 tgtgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGAT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGg‑ccgcc                                                                                                                     >  1:4550489/1‑140 (MQ=17)
                   tgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCCGGGGGCGGCGGCGATTGG‑CCGcccc                                                                                                                   >  2:5639955/1‑140 (MQ=17)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGccccca                                                                                                                 >  1:1892658/1‑139 (MQ=17)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCt                                                                                                                 >  1:3345949/1‑140 (MQ=32)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCt                                                                                                                 >  2:5078753/1‑140 (MQ=32)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCt                                                                                                                 >  1:552731/1‑140 (MQ=32)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCt                                                                                                                 >  2:2966518/1‑140 (MQ=32)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCt                                                                                                                 >  1:6083610/1‑140 (MQ=32)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCt                                                                                                                 >  2:1099902/1‑140 (MQ=32)
                     ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCt                                                                                                                 >  2:4077212/1‑140 (MQ=32)
                      gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTg                                                                                                                >  1:5289777/1‑140 (MQ=32)
                      gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTg                                                                                                                >  1:5289766/1‑140 (MQ=32)
                      gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTg                                                                                                                >  2:1651887/1‑140 (MQ=32)
                          aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGggcgg                                                                                                                                     <  1:3235672/116‑1 (MQ=32)
                          aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGggcgg                                                                                                                                     >  2:3235672/1‑116 (MQ=32)
                          aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTgcgcg                                                                                                            >  1:2313979/1‑140 (MQ=32)
                          aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTgcgcg                                                                                                            >  2:3933365/1‑140 (MQ=32)
                          aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGG‑CCGCCCCCTgcgcg                                                                                                            >  1:748319/1‑140 (MQ=17)
                          aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAGTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTgcgcg                                                                                                            >  1:1717175/1‑140 (MQ=17)
                           gggCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTgcgcgc                                                                                                           >  1:4521600/1‑140 (MQ=32)
                                cgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCtt                                                                                                      >  1:6015688/1‑140 (MQ=32)
                                    ttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTgcgc                                                                                                  >  1:6401026/1‑140 (MQ=32)
                                            ttGTAACCCCGCGCCCGCCCGGGTCCCCTTTAGGGAAAATTCGTGGGCACCGCGCTGCGGC‑TTTTTGTTGCGCATCAGCCGGTGGGTGCGGGGGGCGGCGCCGATTGC‑CCGCCCCTTCCGCGCTCCTTGCGCCAGTGGCa                                                                                          <  2:1445158/139‑1 (MQ=2)
                                            aTGTTCCCTCTCGCCCGACCCGGTAGCCTTATGGTATAATTCGAGGGCACCGCGCTGCGGTTATTTTGTT‑CGCCTGAGCCCGGGGGTGTAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                          <  2:5991515/140‑1 (MQ=11)
                                            aTGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                          >  1:2068910/1‑140 (MQ=34)
                                             tGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGG‑CCGCCCCCAGCGCGCACCTTGCGCCCGTGGCaa                                                                                         >  1:4184332/1‑140 (MQ=255)
                                             tGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCac                                                                                         >  2:2678259/1‑139 (MQ=21)
                                             tGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCaa                                                                                         >  1:5383404/1‑140 (MQ=35)
                                             tGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCaa                                                                                         >  1:271576/1‑140 (MQ=35)
                                             tGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCaa                                                                                         >  1:2684166/1‑140 (MQ=35)
                                               ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAata                                                                                       >  1:1624473/1‑140 (MQ=37)
                                                     cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGTGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCTGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGc                                                                                 >  2:3869837/1‑140 (MQ=255)
                                                     cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCCGTGGCAATATGTTGc                                                                                 >  1:3885149/1‑140 (MQ=255)
                                                     cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGc                                                                                 >  2:6115625/1‑140 (MQ=255)
                                                     cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGc                                                                                 >  2:4525598/1‑140 (MQ=255)
                                                      gcgcCCGACCCGGTTGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCTGGGGGGGGCGGCTATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                <  2:6178482/140‑1 (MQ=255)
                                                      gcgcCCGACCCGGTAGCCGTACGGGATCAGTCGAGGGCACCGCGCAGCGGCGATTTTGGT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                >  2:3728022/1‑140 (MQ=255)
                                                      gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCCGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                >  1:3826816/1‑140 (MQ=255)
                                                      gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                >  1:1373971/1‑140 (MQ=255)
                                                      gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                >  1:5610696/1‑140 (MQ=255)
                                                      gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAAAATGTTTCt                                                                                >  1:4115656/1‑140 (MQ=255)
                                                       cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCtt                                                                               >  1:1706975/1‑140 (MQ=255)
                                                       cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGA‑GGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTa                                                                              >  2:2489518/1‑140 (MQ=255)
                                                                  gTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGaaa                                                                    >  2:4187479/1‑140 (MQ=255)
                                                                        gTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAgcg                                                              >  1:2739640/1‑140 (MQ=255)
                                                                          acgggATATGTCGCGGCCACCTGGCAGCGGCTTTTTTGGT‑CGCCAGAGCCGGGGGGTGACGGGGGTGGCGGCGTTGGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGAGTAGCTCATGAAAGGAGCGca                                                            <  2:2472388/138‑1 (MQ=17)
                                                                             ggATATGTGGTGGGCCCCGCGCAGGGGTTATTTTTTT‑CGCAAGCGCGGGGGGGTGCTGGGGGGGGGGGCGTTTGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGcaaca                                                         <  2:3379903/140‑1 (MQ=18)
                                                                              gATAAGTCGAGGCCACCGCGCAGCGGCGATTTTGTT‑CGCCAGTGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGcaacaa                                                        <  2:1599468/140‑1 (MQ=255)
                                                                               ttAAGTCGAGGGCACCGCGCAGCGGCGTTTTTGTT‑CGCCTGAGCCCGGGGGTGCTGGGGGCGGCGGCGTTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAg                                                       <  2:5614279/139‑1 (MQ=255)
                                                                               aTACGTCGTGGGCACTGGGTAGGGGCTTTTTTGTT‑CGCTATCCCCCGGGGGGGATGGTGGCGGCGTCTTTTGG‑CCGCCACCTGCGCGCTCCTTGCTACAGTGGCAATATGTTGCTTATCTCATGAAAGGATCGCAACAAg                                                       <  2:1483438/140‑1 (MQ=255)
                                                                                tAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAATATTTTGCTTAGCTCATGAAAGGAGCGCAACAAga                                                      >  1:1276109/1‑140 (MQ=255)
                                                                                tAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGG‑CCGCCCCCTGCGCCCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAga                                                      >  1:5542034/1‑140 (MQ=255)
                                                                                 aaGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGGGGCGATTGG‑CGGCCCCCTGCGCGCTCCTTGCGCCAGTGGCTATATGTTGGTTAGCTCATGATAGGAGCGCTACATgat                                                     >  2:2408806/1‑140 (MQ=17)
                                                                                 aaGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgat                                                     >  1:4694926/1‑140 (MQ=255)
                                                                                   gTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatga                                                   >  1:5937567/1‑140 (MQ=255)
                                                                                       aGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGaa                                               >  1:254621/1‑140 (MQ=255)
                                                                                        gggCACCGCGCTGGGGCTTTTTTGTT‑CGCCAGCGCCGGGGGGTGGTGGGGGGGGGGGCGTTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                              <  2:4114867/140‑1 (MQ=18)
                                                                                        gggCACCGCGCTGCGGCGATTTTTTA‑CGCCAGAGCCCGGGGGTGCTGGGGGCGGCGGCGATTGC‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                              <  2:2751295/140‑1 (MQ=18)
                                                                                        gggCACCGCGCAGNGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCCNGAAAGGTGCGCAACAAGATGTTGGAt                                              >  1:5646743/1‑140 (MQ=18)
                                                                                        gggCACCGCGCAGCGGCTATTTTGTT‑CGCCAGAGCCGGGGGGTGCTGGGGGCGGCGGCTATGGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                              <  1:6252441/140‑1 (MQ=18)
                                                                                        ggccACCGCGCGGGGGCTTTTTTGTG‑CGCCAGCGCCGGGGGGTGCTGGGGGGGGGGGCGTTGGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                              <  1:5485809/137‑1 (MQ=18)
                                                                                        gcccccggcgcCGCGGGGATTGTGTT‑GGCCCGCGGCGGGGGGGGGGGGGGGGGGGGGCTCTGGG‑CCCCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                              <  1:4079274/133‑1 (MQ=16)
                                                                                         ggCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATc                                             >  1:883098/1‑140 (MQ=255)
                                                                                         ggCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATc                                             >  2:5450667/1‑140 (MQ=255)
                                                                                             ccGCGCAGCGGTGTTTTTGTT‑CGCCTGAGCCGGGGGGGGCTGGGGGCGGCGGCGTTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACa                                         <  2:1225988/140‑1 (MQ=21)
                                                                                             ccGCGCAGCGGCGATTTTGTT‑CGCCTGAGCCCGGGGGGGCAGGGGGCGGCGGCTATGGG‑CCGCCCCCGGCGCGCGCCGTGCGCCAGTGGCAATATGTTGCTTAGCTCATGCCAGGAGCGCAACAAGCGGATGAATCaccc                                         <  2:4524336/140‑4 (MQ=17)
                                                                                             ccGCGCAGCGGCGATGTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACa                                         >  2:1728626/1‑140 (MQ=255)
                                                                                              cgcgCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                        >  1:4558061/1‑140 (MQ=255)
                                                                                              cgcgCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCGCCTTGCGCCCGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                        >  1:5245899/1‑140 (MQ=255)
                                                                                                cgcAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACatat                                      >  2:4276192/1‑140 (MQ=255)
                                                                                                   cgcggcgATTTGGTG‑CGCCTGAGCCCGGGGGTGCTGGGGGCGGCGGCGATTGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCACTATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaac                                   <  2:413229/139‑1 (MQ=21)
                                                                                                        cgtttttGTT‑CGCCTGAGCCGGGGGGTGCTGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACAt                              <  2:1706975/137‑1 (MQ=255)
                                                                                                        cgATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGTATCAACATATAACAACAt                              >  1:1789118/1‑140 (MQ=255)
                                                                                                                 t‑CGCCAGCGCCCGGGGGTGCCGGGGGCGGCGGCGTGGGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaa                     <  2:740607/140‑1 (MQ=25)
                                                                                                                   cGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaaa                    >  1:1786510/1‑140 (MQ=255)
                                                                                                                      cAGAGCCGGGGGGTGTCGGGGGGGGCGGTGATTGG‑CCCCCCCTGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGc                 <  2:2167455/140‑1 (MQ=37)
                                                                                                                       tgagCTCGGGGGTGCTGGGGGGGGCGGCGATTGT‑CCGCCCCGTGCGCGCTCCTTGCGACAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                <  2:1387947/139‑1 (MQ=255)
                                                                                                                        gcgcgggggggTGC‑GGGGGGGGGGGGGTTGGGCCCCCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt               <  1:3976531/134‑1 (MQ=255)
                                                                                                                        gcgCCGGGGGGTGTTGGGGGCGGGGGGGATGGG‑CCGCCCCCGGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt               <  1:6166378/138‑1 (MQ=255)
                                                                                                                        gacccggggggTGCCGGGGGCGGCGGCTCTGGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt               <  1:6166336/134‑1 (MQ=37)
                                                                                                                         agCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATTTTGCTTAGCTCATGTAAGGAGCGCAACAATATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTg              >  1:1026648/1‑140 (MQ=255)
                                                                                                                            cggggggTGCTGGGGGGGGGGGCTTTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACa           <  2:254621/138‑1 (MQ=37)
                                                                                                                              gggggTGCAGGGGGCGGCGGCGTTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCAAGTGGCAATATGTTGCTTAGGTCATGGAAGGAGCGCAACAAGATGATGCAGCAACATAGAACCAGAGCTTAAAAAAAGCCCTGAcctg         >  2:6446700/1‑137 (MQ=21)
                                                                                                                                    gCAGGGGGCGGCGGCGATTGG‑CCGCCCCCGGCGCGCGCCATGCGCCAGTGGCAAAATGTTGCTTATCTAATGAAAGGAGCGCAACAAGAAGATGAATAAACATATAACAACATCTTAAAAAAATGCCTGAAATTACGCCa   >  1:1010410/1‑140 (MQ=37)
                                                                                                                                     cAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCTGGGGCAATATGTTGCTTAGCTCATGAATGGAGCGCAACAAGATGATGAATCACCATATCACACCAACTGAAAAAATGGCCTGACATGTCGCCAg  >  2:5946496/1‑140 (MQ=25)
                                                                                                                                     cAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAg  >  1:3606372/1‑140 (MQ=255)
                                                                                                                                     cAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAg  >  1:3606173/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                           
GGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTT‑CGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAG  >  NZ_CP025268/922173‑922444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑