BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	541,580	G→A	A215T (GCG→ACG)	glxK →	glycerate 3‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	541,580	0	G	A	98.6%	1344.8 / NA	353	A215T (GCG→ACG)	glxK	glycerate 3‑kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (200/146); minor base C (1/4); total (201/152)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 359 total aligned reads displayed.

GCGCGTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATT  >  NZ_CP025268/541444‑541716

gcgcgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGTAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAacgc                                                                                                                                       >  2:3910145/1‑140 (MQ=255)

gcgcgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAacgc                                                                                                                                       >  1:5560237/1‑140 (MQ=255)

 cgcgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGcc                                                                                                                                      >  2:2089845/1‑140 (MQ=255)

   cgTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCgg                                                                                                                                    >  1:3528606/1‑140 (MQ=255)

     tGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGgag                                                                                                                                  <  1:2254387/140‑1 (MQ=255)

     tGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCAATATTGAAGTAGCATGTGACGTTGATTACCCGCTGGTAGGGGCACGCGGCGCGGCGGCTGTGTTTGGCCCGCAAAAAGGGGCAACGCCGtag                                                                                                                                  <  2:3064103/140‑1 (MQ=255)

      ggCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGgaga                                                                                                                                 <  1:4358860/140‑1 (MQ=255)

            cATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCg                                                                                                                           >  1:1605551/1‑140 (MQ=255)

             aTTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCga                                                                                                                          <  2:1668838/140‑1 (MQ=255)

             aTTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCga                                                                                                                          <  1:140886/140‑1 (MQ=255)

              ttGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCgaa                                                                                                                         >  1:5116459/1‑140 (MQ=255)

                     ggatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTg                                                                                                                  >  1:5748651/1‑140 (MQ=255)

                      gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCACAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGa                                                                                                                 >  1:3078461/1‑140 (MQ=255)

                           aTGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                                            >  1:3783086/1‑140 (MQ=255)

                           aTGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                                            <  1:4952224/140‑1 (MQ=255)

                            tGCTATCCGCGTCTGGCGAATTGAAATATTGAAGTAGCATGTGACGTTGATAAACCGCTGGGAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCACGCAAAAAGGGGCAACGCCGCAGATGGTCGAAGAACTTGAACAgtg                                                                                                           >  2:4640635/1‑138 (MQ=255)

                            tGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAggg                                                                                                           >  2:6398027/1‑140 (MQ=255)

                                 tCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                                      <  2:3455347/140‑1 (MQ=255)

                                     cgTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCaaaa                                                                                                  >  2:5266497/1‑140 (MQ=255)

                                        cTGGCGACTGGCCAGATTGAAGTCGCATGTGCCGTTGATAACCCGCGGGGAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAACACGGGGCAACGCCGGCGATGGTCGCAGAACTGGAACCGGGGCTGCAAAAGTa                                                                                               <  2:5781554/140‑1 (MQ=255)

                                          ggCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACg                                                                                             >  1:1026801/1‑140 (MQ=255)

                                           gCGAATTGCCATATTGAAGTAGCATGTGACGTTGATACCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGc                                                                                            <  1:4206880/140‑1 (MQ=255)

                                           gCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGc                                                                                            <  1:5309500/140‑1 (MQ=255)

                                              aaTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                                         <  1:2588203/140‑1 (MQ=255)

                                               aTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCgt                                                                                        >  2:3695451/1‑140 (MQ=255)

                                               aTTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCgt                                                                                        >  2:1476353/1‑140 (MQ=255)

                                                ttGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCgtg                                                                                       <  1:2384591/140‑1 (MQ=255)

                                                        ttGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTacaa                                                                               >  2:5304018/1‑140 (MQ=255)

                                                          gAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCTGCGGCGGTGTTTGGCCCGCAAAAAGGTGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCACGTGTTTTacaaca                                                                             <  2:2466506/140‑1 (MQ=255)

                                                           aaGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAg                                                                            >  1:1452989/1‑140 (MQ=255)

                                                             gTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCa                                                                          >  1:2606350/1‑140 (MQ=255)

                                                             gTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCa                                                                          >  2:871053/1‑140 (MQ=255)

                                                              tAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCaa                                                                         <  2:4443823/140‑1 (MQ=255)

                                                                gCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                                       >  1:334463/1‑140 (MQ=255)

                                                                gCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                                       >  2:3658735/1‑140 (MQ=255)

                                                                 cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACt                                                                      >  1:6306550/1‑140 (MQ=255)

                                                                  aTGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                                     >  2:1171627/1‑140 (MQ=255)

                                                                  aTGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTGTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                                     >  2:3755635/1‑140 (MQ=255)

                                                                   tgtgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGa                                                                    >  2:1542544/1‑140 (MQ=255)

                                                                     tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAATAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                                  >  2:3595375/1‑140 (MQ=255)

                                                                     tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGACGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                                  >  1:6195214/1‑140 (MQ=255)

                                                                     tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                                  >  2:2606044/1‑140 (MQ=255)

                                                                          ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAt                                                             >  1:3902770/1‑140 (MQ=255)

                                                                            gATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTACTGt                                                           <  2:1064023/140‑1 (MQ=255)

                                                                             aTAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTc                                                          >  2:957352/1‑140 (MQ=255)

                                                                               aaCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTg                                                        >  1:1284580/1‑140 (MQ=255)

                                                                                 cccGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGcc                                                      >  1:638298/1‑140 (MQ=255)

                                                                                 cccGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGGTTGGCCCGCAATAAGGGGCAACGCCGGAGATGGTCGATGAACTTGAACTGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCCAACTGACATTAATGTCTGcc                                                      >  2:4302136/1‑140 (MQ=255)

                                                                                    gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                                                   >  2:6106853/1‑140 (MQ=255)

                                                                                    gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                                                   >  1:2086725/1‑140 (MQ=255)

                                                                                    gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                                                   >  2:3381139/1‑140 (MQ=255)

                                                                                     cTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                                                  >  1:1062273/1‑140 (MQ=255)

                                                                                     cTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                                                  >  1:6401786/1‑140 (MQ=255)

                                                                                      tGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATg                                                 >  1:1819113/1‑140 (MQ=255)

                                                                                       ggTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCga                                                                                                                          >  2:2224447/1‑66 (MQ=255)

                                                                                         tAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcg                                              <  2:2765780/140‑1 (MQ=255)

                                                                                             ggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggcg                                          >  1:1528017/1‑140 (MQ=255)

                                                                                              gCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                                         >  1:161505/1‑140 (MQ=255)

                                                                                                  gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                     >  2:4112454/1‑140 (MQ=255)

                                                                                                  gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                     <  2:4982859/140‑1 (MQ=255)

                                                                                                  gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGGTTTACAACAGCCAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                     >  2:6160050/1‑140 (MQ=255)

                                                                                                   cggcgcggcgGCGGTGTTTGGCCCGCACAAAGGGGCACCGCCGGAGATGGTCGACGAACTTGCACAGGGGCTGCTATCTGACGCCCGTGTTTTACATCAGAATACTGACATTTTTGTCTGCCAGTTGGCGGgcggcggcg                                    >  2:1489915/1‑140 (MQ=255)

                                                                                                      cgcggcgGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTg                                 <  1:846169/140‑1 (MQ=255)

                                                                                                        cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg                               <  2:129681/140‑1 (MQ=255)

                                                                                                        cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg                               >  2:4947029/1‑140 (MQ=255)

                                                                                                         ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                              <  1:4629363/140‑1 (MQ=255)

                                                                                                         ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                              <  1:1684173/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAACGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                             <  2:1812360/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                             <  1:962714/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                             <  1:2021275/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                             <  1:2032110/140‑1 (MQ=255)

                                                                                                          gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCGGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                             <  2:1907014/140‑1 (MQ=255)

                                                                                                           cggctgTGTTTGGCCCGCAAAAAGGTGCACCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCAGCAAAACTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAAGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                            <  2:3304983/140‑1 (MQ=255)

                                                                                                           cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                            <  2:5681664/140‑1 (MQ=255)

                                                                                                           cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                            <  2:2928242/140‑1 (MQ=255)

                                                                                                           cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                            <  1:5864403/140‑1 (MQ=255)

                                                                                                           cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                            <  2:4639686/140‑1 (MQ=255)

                                                                                                           cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                            <  1:1755537/140‑1 (MQ=255)

                                                                                                           cggcggAGTTTTGCCCGCTAAAAGGGTCAACGACGGAGATTGTCGAAGATCTTGAACAGGGTCTGCATATGTAAGCCCGTGTTTTACAACCGCAATCTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGATgcgggc                            <  2:6348973/140‑1 (MQ=255)

                                                                                                              cggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt                         <  1:2804886/140‑1 (MQ=255)

                                                                                                              cggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt                         <  1:2914339/140‑1 (MQ=255)

                                                                                                              cggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt                         <  1:3598353/140‑1 (MQ=255)

                                                                                                                gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt                       >  1:4834212/1‑140 (MQ=255)

                                                                                                                gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGAGGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt                       <  2:423701/140‑1 (MQ=255)

                                                                                                                gtgtTTGGCCCGCAAAAAGGGGCAACGCCGGAGAGGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt                       <  1:6241815/140‑1 (MQ=255)

                                                                                                                gtgaTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt                       <  2:4780568/140‑1 (MQ=255)

                                                                                                                     tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                              >  1:1990265/1‑128 (MQ=255)

                                                                                                                     tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                              <  2:1990265/128‑1 (MQ=255)

                                                                                                                     tGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTGTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAGGGGTa                  >  2:5950648/1‑140 (MQ=255)

                                                                                                                      ggCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTAt                 <  1:6087751/140‑1 (MQ=255)

                                                                                                                        cccGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTg               >  1:3721382/1‑140 (MQ=255)

                                                                                                                           gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCTGCGGCGCTGCGGGCGGTATGGGTATTgcgg            >  2:5622380/1‑140 (MQ=255)

                                                                                                                           gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTCTGGGTATTgcgg            >  2:1930929/1‑140 (MQ=255)

                                                                                                                           gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg            >  1:1199316/1‑140 (MQ=255)

                                                                                                                           gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg            >  2:1381359/1‑140 (MQ=255)

                                                                                                                           gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg            >  1:3184370/1‑140 (MQ=255)

                                                                                                                           gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg            >  1:2746261/1‑140 (MQ=255)

                                                                                                                            cAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggc           >  1:1870949/1‑140 (MQ=255)

                                                                                                                              aacaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggcgg         <  1:6131926/137‑1 (MQ=255)

                                                                                                                                aaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggcggcg       <  2:671033/140‑1 (MQ=255)

GCGCGTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTATT  >  NZ_CP025268/541444‑541716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`