BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	71,946	A→G	255 (TAA→TAG)	yabI →	DedA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	71,946	0	A	G	99.1%	1184.2 / NA	345	255 (TAA→TAG)	yabI	DedA family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (110/232); minor base T (3/0); total (113/232)
Fisher's exact test for biased strand distribution p-value = 3.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

CATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTG  >  NZ_CP025268/71809‑72082

 attattTGTCCCGCGGTCGTGTTTTTTTGCAGACGCCCTTGATTTCATCCAACGGCGTGGTGGCGCAGGTGGTGTTAATTAGCCCCCCCTTTATTCCCGTGTAAATCGATAATTTGCGTAAAGTGGTTGGGGTTTAggag                                                                                                                                       >  1:1640538/1‑140 (MQ=255)

    attTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAACGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATa                                                                                                                                    >  1:6128060/1‑140 (MQ=255)

         tCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTGTAGGAGATAGTCtt                                                                                                                               >  2:2791780/1‑140 (MQ=255)

           ccGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTtgt                                                                                                                             <  2:1732454/140‑1 (MQ=255)

            cgcgGTGGTTTGTTGTGGCTGTCGCCGTTGTTTTCTGCCTTCGGCGGGGTGGCGCTGGTGGTGTTTATTCGCCACCCGTTGATGCCGGTGTATCTCGGGATTTTGCGGTAAGGGGTTGGGGTTTAGGAGATAGTCTtgtg                                                                                                                            >  2:2711224/1‑140 (MQ=255)

             gcgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTTCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCACCCGTTGATGCCGGTGTATATAGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTTTGc                                                                                                                           >  2:730546/1‑140 (MQ=255)

              cgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAACGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGGGGATATAGATATATTTCGTAAATTGGTTTGGGTTTAGGCGATAGTCTTGTGCg                                                                                                                          >  2:3646646/1‑140 (MQ=255)

                     ttgttgTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGcc                                                                                                                   <  1:1899823/140‑1 (MQ=255)

                     gtgttgaGGCTGACGCCGGGGCTTTCTGCCCTCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGGTGATGCCGGTGTATAACGAAATTGTGCGTAAAGTGGTTGGGGTATAGGAGATCGTCGTGTGCGGGGTGcc                                                                                                                   <  2:1797323/139‑1 (MQ=255)

                           tgGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGc                                                                                                             >  2:4319394/1‑140 (MQ=255)

                               tGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACg                                                                                                         <  1:3919945/140‑1 (MQ=255)

                               tGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACg                                                                                                         <  1:3945381/140‑1 (MQ=255)

                               tGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACg                                                                                                         <  2:6336451/140‑1 (MQ=255)

                               tGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTGTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACg                                                                                                         <  1:213169/140‑1 (MQ=255)

                                  cGCCGGTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCtt                                                                                                      >  1:5096957/1‑140 (MQ=255)

                                       ttGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgc                                                                                                 <  1:4737104/140‑1 (MQ=255)

                                              cTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                          <  2:6063294/140‑1 (MQ=255)

                                              cTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                          <  2:4095197/140‑1 (MQ=255)

                                              cTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                          <  1:5779222/140‑1 (MQ=255)

                                               tGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                         >  2:4711816/1‑140 (MQ=255)

                                               tGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                         >  2:2146938/1‑140 (MQ=255)

                                                 ccATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                       >  2:205445/1‑140 (MQ=255)

                                                 ccATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                       >  2:502618/1‑140 (MQ=255)

                                                 ccATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTGTAGGAGAGAGTCTTGTGCGGGTTGCCTGATGCGTCGCGTGCCGCGGCtgagctg                                                                                       >  1:6027913/1‑134 (MQ=255)

                                                   ctCGGCGGGGTGGCGCTGGTGGTGTTAAGTCGCCACCCGTTGATGCCGGTGTATATCGCTATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                     <  2:1017652/139‑1 (MQ=255)

                                                   aTCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                     <  1:371413/140‑1 (MQ=255)

                                                    tCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                    >  2:5916007/1‑140 (MQ=255)

                                                    tCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                    >  1:3250075/1‑140 (MQ=255)

                                                     cGGCGTTGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAATTTGCGTAAAGTGGTTGGGGTATAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                   <  2:2527477/140‑1 (MQ=255)

                                                     cGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                   <  2:3435513/140‑1 (MQ=255)

                                                         gtggtgGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaa                                                                               <  2:4427364/140‑1 (MQ=255)

                                                         gtggtgGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaa                                                                               <  1:1798726/140‑1 (MQ=255)

                                                             tggCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACg                                                                           <  1:1091403/140‑1 (MQ=255)

                                                               gcgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                                         <  1:5405578/140‑1 (MQ=255)

                                                               gcgcTGGTGGTGTTAAGTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                                         <  1:357622/140‑1 (MQ=255)

                                                                cgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCAc                                                                        <  2:2273823/140‑1 (MQ=255)

                                                                cgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCAc                                                                        <  2:5511771/140‑1 (MQ=255)

                                                                  cTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTa                                                                      >  2:1357540/1‑140 (MQ=255)

                                                                  cTGGTGGGGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGAGATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTa                                                                      >  2:5318252/1‑140 (MQ=255)

                                                                     gtggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTAccc                                                                   <  1:2972842/140‑1 (MQ=255)

                                                                      tggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCg                                                                  <  1:6269880/140‑1 (MQ=255)

                                                                       ggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGt                                                                 <  1:1352347/140‑1 (MQ=255)

                                                                           ttAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGt                                                             <  1:2992950/140‑1 (MQ=255)

                                                                           ttAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGt                                                             <  2:3158555/140‑1 (MQ=255)

                                                                           ttAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTGTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGt                                                             <  2:1583038/140‑1 (MQ=255)

                                                                                  gCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATaa                                                      >  2:1074418/1‑140 (MQ=255)

                                                                                   ccACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                                    >  1:1003309/1‑93 (MQ=255)

                                                                                        cGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGtt                                                >  1:343229/1‑140 (MQ=255)

                                                                                            gATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcg                                            <  1:2753794/140‑1 (MQ=255)

                                                                                              tGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcgcc                                          <  1:4893193/140‑1 (MQ=255)

                                                                                              tGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcgcc                                          <  1:945103/140‑1 (MQ=255)

                                                                                               gCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTGTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcgccg                                         <  1:46279/140‑1 (MQ=255)

                                                                                                 cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCa                                       <  1:2850785/140‑1 (MQ=255)

                                                                                                 cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCa                                       >  1:2016261/1‑140 (MQ=255)

                                                                                                     gtATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCg                                   <  1:3301571/140‑1 (MQ=255)

                                                                                                     gtATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGC‑‑‑‑‑‑‑‑CGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCa                           <  2:3966660/140‑1 (MQ=25)

                                                                                                      tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                  <  2:4506448/140‑1 (MQ=255)

                                                                                                      tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                  <  1:5934206/140‑1 (MQ=255)

                                                                                                      tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                  <  1:1206653/140‑1 (MQ=255)

                                                                                                      tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTAGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                  <  1:4399363/140‑1 (MQ=255)

                                                                                                      gagatCGATCTTTTGCGTAAAGTGGTTGCGGTTTAGGAGCTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                  <  2:380967/137‑1 (MQ=37)

                                                                                                        tatCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACa                                >  2:6283537/1‑140 (MQ=255)

                                                                                                        tatCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACa                                >  2:3012726/1‑140 (MQ=255)

                                                                                                         atCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                          >  2:6014367/1‑81 (MQ=255)

                                                                                                          tCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGt                              <  1:2608416/140‑1 (MQ=255)

                                                                                                          tCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGt                              <  1:710682/140‑1 (MQ=255)

                                                                                                          tCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGt                              <  1:5618169/140‑1 (MQ=255)

                                                                                                          tCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGt                              <  1:1546852/140‑1 (MQ=255)

                                                                                                           cGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTg                             <  2:4930643/140‑1 (MQ=255)

                                                                                                             atatTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCa                           >  2:2003147/1‑140 (MQ=255)

                                                                                                             atatTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCa                           >  1:6168460/1‑140 (MQ=255)

                                                                                                             atatTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCa                           >  2:2942646/1‑140 (MQ=255)

                                                                                                              tatTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCat                          >  1:5699200/1‑140 (MQ=255)

                                                                                                               atTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCata                         <  1:1686430/140‑1 (MQ=255)

                                                                                                               atTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCata                         <  2:5699200/140‑1 (MQ=255)

                                                                                                                ttttGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                  <  2:6166927/130‑1 (MQ=255)

                                                                                                                 tgagCGTACAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGAAGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCatact                       <  1:3821750/137‑3 (MQ=37)

                                                                                                                  ttGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTa                      >  1:2229839/1‑140 (MQ=255)

                                                                                                                  ttGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTa                      <  1:3616042/140‑1 (MQ=255)

                                                                                                                   tGCGGAAAGGGGTTGGGGTGTAGGTGCTTGTGTTGGGCGGGGTGCCTGATGCGACGGTTGCCGCGTCTTAGCTGGGCTTCAATTCGCTCTACCGGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTag                     >  2:326273/1‑139 (MQ=19)

                                                                                                                    gCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAAc                    <  1:4319394/140‑1 (MQ=255)

                                                                                                                     cGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAAcc                   >  2:1629123/1‑140 (MQ=255)

                                                                                                                       tAAAGTGGTTGGGGTTTAGGATATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGTCCTACAAAACGCACTACCCGTATGTCGGATAAGGATTTCACGCCGCATCCGACAGTGCATACTAACCCg                 <  2:1137563/140‑1 (MQ=37)

                                                                                                                       tAAAGTGGTTGGGGTTTAGGATATAGTCTGGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTCCCCGTAGGTCGGATAAGGCGTTCACGCCGCAACCGACAGTGCATACTAACCCg                 <  2:801160/140‑1 (MQ=37)

                                                                                                                       tAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCg                 <  1:835173/140‑1 (MQ=255)

                                                                                                                       tAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCg                 <  1:4982791/140‑1 (MQ=255)

                                                                                                                       tAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCg                 <  1:4198767/140‑1 (MQ=255)

                                                                                                                       tAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCg                 <  1:2412010/140‑1 (MQ=255)

                                                                                                                       tAAAGTGGTGGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCg                 <  2:4565943/140‑1 (MQ=255)

                                                                                                                       gaaaGTGGTTGGGGTTTAGGAGATCGTCTTGTGCGGGTTGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCTCTTCCCGTAGGTCGGATAAGGGGTTCTCGCCGCATCCGACAGTGCATACTAACCCg                 <  2:5606672/139‑1 (MQ=37)

                                                                                                                         aaGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa               <  2:4181282/140‑1 (MQ=255)

                                                                                                                         aaGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa               <  1:2575911/140‑1 (MQ=255)

                                                                                                                         aaGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa               <  1:2489542/140‑1 (MQ=255)

                                                                                                                            tGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATc            <  2:5418143/140‑1 (MQ=255)

                                                                                                                            tGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATc            <  1:3157137/140‑1 (MQ=255)

                                                                                                                              gTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATccc          >  1:6378055/1‑140 (MQ=255)

                                                                                                                                 ggggTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCaa       <  2:5825468/140‑1 (MQ=255)

                                                                                                                                 ggggTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCaa       <  1:4164853/140‑1 (MQ=255)

                                                                                                                                 ggggTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCaa       <  2:6255396/140‑1 (MQ=255)

                                                                                                                                 ggggTTTAGGAGAGAGACTTGGGCGGGTTGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACAAACCGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCCTCCGACAGTGCATACTACCCCGTAATCCCCaa       <  2:5337279/140‑1 (MQ=25)

CATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTG  >  NZ_CP025268/71809‑72082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`