BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	663,151	T→C	363 (TAA→TAG)	rlpA ←	endolytic peptidoglycan transglycosylase RlpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	663,151	0	T	C	100.0%	1391.2 / NA	398	363 (TAA→TAG)	rlpA	endolytic peptidoglycan transglycosylase RlpA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (218/180); total (218/180)

Only 100 of 399 total aligned reads displayed.

GTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGG  >  NZ_CP025268/663014‑663287

gTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTa                                                                                                                                        <  2:6070355/140‑1 (MQ=255)

 tCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTAc                                                                                                                                       <  2:208680/140‑1 (MQ=255)

  cTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACt                                                                                                                                      >  2:3112525/1‑140 (MQ=255)

  cTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACt                                                                                                                                      >  2:1438906/1‑140 (MQ=255)

  cTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACt                                                                                                                                      >  2:5731339/1‑140 (MQ=255)

   tGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTg                                                                                                                                     >  1:5472244/1‑140 (MQ=255)

    gAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCTAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTgc                                                                                                                                    <  1:3069017/140‑1 (MQ=255)

     aaCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTgcg                                                                                                                                   <  1:1098553/140‑1 (MQ=255)

     aaCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTgcg                                                                                                                                   <  2:2460779/140‑1 (MQ=255)

     aaCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTgcg                                                                                                                                   <  2:738953/140‑1 (MQ=255)

     aaCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACGATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTgcg                                                                                                                                   <  2:2670456/140‑1 (MQ=255)

         aCGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGgt                                                                                                                               <  1:664273/140‑1 (MQ=255)

         aCGACATCAGTGATGGAATTAAAAAAGTGCCCTACTAGAGCAAATGCACTACCGGCAGTCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGgt                                                                                                                               <  2:5632875/140‑1 (MQ=255)

          cGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGgta                                                                                                                              >  2:5258230/1‑140 (MQ=255)

          cGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGgta                                                                                                                              >  1:410953/1‑140 (MQ=255)

          cGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGgta                                                                                                                              >  1:2871541/1‑140 (MQ=255)

          cGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGgta                                                                                                                              >  2:2356885/1‑140 (MQ=255)

          cGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGgta                                                                                                                              >  1:1450576/1‑140 (MQ=255)

           gACATCCGTGATGGAATTAAAAAAGTGCCCTAATATAGCAACTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGgtag                                                                                                                             <  2:1379693/140‑1 (MQ=255)

               tCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGtaat                                                                                                                         >  1:893276/1‑140 (MQ=255)

               tCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGtaat                                                                                                                         >  2:4248847/1‑140 (MQ=255)

               tCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGtaat                                                                                                                         >  2:3527137/1‑140 (MQ=255)

               tCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACGTTGTTAACGTCATTTACAGAAATTGACACGTCAGTTGCCTGCTTTACGCTACTGCGCGGTAGtaat                                                                                                                         >  2:5857915/1‑140 (MQ=255)

               tCCGTGATGGAATTAAAAAAGTGCCCTACTATAGAAATTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTTACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGtaat                                                                                                                         >  2:6451403/1‑140 (MQ=255)

               tCCGTGATGGAATTAAAAAAGAGCCCTACTATAGCAAATGCACTACCGGCAGGAATCTGACTTTCAGCGTGACTTTTTTAACGTCATTTACAGAAATTGAAACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGtaat                                                                                                                         >  1:1184939/1‑140 (MQ=255)

                ccGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGtaata                                                                                                                        >  2:6002026/1‑140 (MQ=255)

                ccGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGtaata                                                                                                                        <  2:3021358/140‑1 (MQ=255)

                  gTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTCATaaa                                                                                                                      >  2:293996/1‑140 (MQ=255)

                  gTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATaaa                                                                                                                      <  1:1727492/140‑1 (MQ=255)

                   tGATGGAATTAAAACAGTGCCCTACTATAGCACATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACATTGTTAACGTCATTTACAGAAATTGCCACATCAGAGGCCTGCTTTACGCTACTGCGCGGTAGTAATAAAt                                                                                                                     <  2:1979115/140‑1 (MQ=255)

                   tGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAAt                                                                                                                     <  1:3562274/140‑1 (MQ=255)

                   tGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAAt                                                                                                                     <  2:1162622/140‑1 (MQ=255)

                   tGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAAt                                                                                                                     <  2:4063918/140‑1 (MQ=255)

                      tGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGAc                                                                                                                  <  2:2878993/140‑1 (MQ=255)

                         aaTTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGt                                                                                                               >  2:6150525/1‑140 (MQ=255)

                         aaTTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGt                                                                                                               >  2:3937178/1‑140 (MQ=255)

                            tAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAAt                                                                                                            <  1:608560/140‑1 (MQ=255)

                                  aGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCt                                                                                                      <  1:375777/140‑1 (MQ=255)

                                     gCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCg                                                                                                   <  1:4851476/140‑1 (MQ=255)

                                     gCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCg                                                                                                   >  1:4461641/1‑140 (MQ=255)

                                              taGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCaaa                                                                                          >  1:6402087/1‑140 (MQ=255)

                                              taGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCaaa                                                                                          >  1:2364163/1‑140 (MQ=255)

                                                 cAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTCGTACTAAATGACTGTAATTGGGCTTCGGTTTGCAAACGt                                                                                       >  1:5891829/1‑140 (MQ=255)

                                                 cAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGt                                                                                       >  1:4654356/1‑140 (MQ=255)

                                                 cAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGt                                                                                       >  2:1952062/1‑140 (MQ=255)

                                                 cAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGt                                                                                       >  1:958928/1‑140 (MQ=255)

                                                 cAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTGCGGTTTGAAAACGt                                                                                       >  2:1745757/1‑140 (MQ=255)

                                                 cAAAGGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTGTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGGCATTGGGCTTCGGTTTGCAAACGt                                                                                       >  2:5444246/1‑140 (MQ=255)

                                                   aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtg                                                                                     <  1:1250817/140‑1 (MQ=255)

                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgct                                                                                   >  1:430469/1‑140 (MQ=255)

                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgct                                                                                   >  2:3308007/1‑140 (MQ=255)

                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgct                                                                                   >  1:3339651/1‑140 (MQ=255)

                                                     tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgct                                                                                   >  2:4330630/1‑140 (MQ=255)

                                                     tGCACTACCGGCAGGCATCTGACTTGCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgct                                                                                   >  2:2834615/1‑140 (MQ=255)

                                                      gCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGTAATTGACACATCAGATGCGTGCTTTACGCTACTGCGCGGTAGTAATAAATGTCTGTAATTGGGCTTCGGTTTGCAAACGTtgctg                                                                                  >  2:4840912/1‑140 (MQ=255)

                                                        aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGNTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                                <  1:5686070/140‑1 (MQ=255)

                                                        aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTCATAATTGACTGTCATTGGGCTTCGGTTTGCAATCGTtgctgct                                                                                >  2:3228184/1‑139 (MQ=255)

                                                        aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                                >  2:3899575/1‑140 (MQ=255)

                                                        aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                                <  2:5566499/140‑1 (MQ=255)

                                                        aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                                <  2:900115/140‑1 (MQ=255)

                                                        aCTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                                <  1:2542151/140‑1 (MQ=255)

                                                         cTACCGGCAGGCATCTGTCTTTCCGCGTGACTGGGTTAACGTCTTTGACAGAAATTGACACATCAGATGCCTGCTTTTCGCTCCTGCGCGGTAGTAATAAATGTCTGTAAGTGGGCTTCGGTGTGGAAACGTTGCTGCat                                                                               >  2:2248853/1‑139 (MQ=255)

                                                          tACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAg                                                                              >  2:378644/1‑140 (MQ=255)

                                                           aCCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAgg                                                                             >  1:3260148/1‑140 (MQ=255)

                                                             cGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTa                                                                           <  1:2116250/140‑1 (MQ=255)

                                                               gcaggcaTCTGACTTTCCGCGTGACTTTGTTAACGTCCTTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACt                                                                         >  1:536386/1‑140 (MQ=255)

                                                                    caTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCtt                                                                    >  1:1000289/1‑140 (MQ=255)

                                                                    caTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCtt                                                                    >  1:866023/1‑140 (MQ=255)

                                                                    caTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCtt                                                                    >  1:1597146/1‑140 (MQ=255)

                                                                    caTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGGTGCTGCAAGGTACTGGCtt                                                                    >  1:48980/1‑140 (MQ=255)

                                                                        tGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGc                                                                >  1:1491879/1‑140 (MQ=255)

                                                                         gACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCt                                                               >  2:1863139/1‑140 (MQ=255)

                                                                                    tGACTTGGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGaa                                                    <  2:5127958/140‑1 (MQ=255)

                                                                                         ttGTTAACGTCATGTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATACATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGc                                               <  2:335344/140‑1 (MQ=255)

                                                                                             tAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAg                                           <  1:4029395/140‑1 (MQ=255)

                                                                                             tAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAg                                           <  1:1391336/140‑1 (MQ=255)

                                                                                                 gTCATTTACAGAACTGGACACATCAGATGCCTGCTTTCCGCTACCGCGCGGTAGTAATAAATGACTGTACTTGGGCTTCGGAGTGCAACCGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                                       <  2:2526485/140‑1 (MQ=255)

                                                                                                 gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                                       <  2:2157078/140‑1 (MQ=255)

                                                                                                 gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                                       <  1:2740460/140‑1 (MQ=255)

                                                                                                 gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                                       <  1:6215177/140‑1 (MQ=255)

                                                                                                 gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                                       <  2:1085952/140‑1 (MQ=255)

                                                                                                 gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGGTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                                       <  1:4372427/140‑1 (MQ=255)

                                                                                                    aTTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATc                                    >  1:4553830/1‑140 (MQ=255)

                                                                                                      ttACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCg                                  >  1:3843124/1‑140 (MQ=255)

                                                                                                              aTTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCGTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACcg                          >  2:4592160/1‑140 (MQ=255)

                                                                                                               ttGACACCTCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACcgc                         <  2:1498408/140‑1 (MQ=255)

                                                                                                                      aTCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCAttt                  >  1:5219813/1‑140 (MQ=255)

                                                                                                                          gATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAg              >  1:106675/1‑140 (MQ=255)

                                                                                                                          gATGCCTGCTGTACGCTACTGCGCGGTAGTACTAAATGACTGTAAGTGGGCTTCGGTTTGCCAACGTTGCTGCAAGGTACTGGCTTCGGCGTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCAGTTTGAg              >  2:2227991/1‑140 (MQ=255)

                                                                                                                           aTGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCGTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTGTGCTGGCGAAGGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGt             >  2:1205518/1‑140 (MQ=255)

                                                                                                                             gCCTGCTTTTCGCTACTGCGCGGGAGTAATATATGACGTTAATTGGGCTTGGGTGAGCAACAGTTGCTTCAAGGTACGGGCTTCGGCTTTGCTGGAGTTTGGGCCAAGCTGGATCCGCCAGAGCGCGCCATTTTGTGTTa           <  2:4475536/140‑1 (MQ=255)

                                                                                                                              ccatatttACGCTACTGCGCGGTAGAAATAAAAGACTGTAATTGTGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGACAAATTTAGTTAc          >  2:6303792/6‑140 (MQ=255)

                                                                                                                               cTGCTTTACGCTACTGCGCGGTATTAATAAATGACTGTAATTGTGCTTCGGTTTGCAAACGTTGCTGCTAGGTTCTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTAcg         <  2:5779937/140‑1 (MQ=255)

                                                                                                                               cTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTAcg         <  1:3633637/140‑1 (MQ=255)

                                                                                                                               cTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTAcg         <  1:2212231/140‑1 (MQ=255)

                                                                                                                                tGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTAcgc        >  2:6118139/1‑140 (MQ=255)

                                                                                                                                  cTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGa      >  1:3503263/1‑140 (MQ=255)

                                                                                                                                  cTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGa      >  1:1857517/1‑140 (MQ=255)

                                                                                                                                     tACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCg   >  1:2033440/1‑140 (MQ=255)

                                                                                                                                      aCGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCgg  >  1:562527/1‑140 (MQ=255)

GTCTGAACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCGACCGG  >  NZ_CP025268/663014‑663287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`