BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	833,226	T→C	378 (TAA→TAG)	ybhS ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	833,226	0	T	C	99.5%	1310.0 / NA	379	378 (TAA→TAG)	ybhS	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (180/196); minor base A (2/0); total (182/197)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 379 total aligned reads displayed.

GCGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGA  >  NZ_CP025268/833089‑833362

     gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                   >  1:3820002/1‑140 (MQ=255)

     gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                   >  2:3766338/1‑140 (MQ=255)

      cggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAg                                                                                                                                  <  1:3419054/140‑1 (MQ=255)

       ggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGc                                                                                                                                 <  1:2675063/140‑1 (MQ=255)

              gggAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGtt                                                                                                                          <  1:1542315/140‑1 (MQ=255)

                gAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTg                                                                                                                        >  1:536544/1‑140 (MQ=255)

                      gATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCNACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTc                                                                                                                  >  1:5683343/1‑140 (MQ=255)

                        tCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAg                                                                                                                <  1:3004555/140‑1 (MQ=255)

                          aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGcc                                                                                                              >  1:1732348/1‑140 (MQ=255)

                          aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTATGCAGAGTAACGTCCATACGCGATGAAACAAGCTCTTCTCCCTAATCCAGACGACGTTTGGTTTTCAGcc                                                                                                              >  2:4410161/1‑140 (MQ=255)

                           ccTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCa                                                                                                             >  2:6073385/1‑140 (MQ=255)

                              gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                          >  2:4536011/1‑140 (MQ=255)

                              gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                          <  2:6201974/140‑1 (MQ=255)

                              gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCGTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                          >  2:2846293/1‑140 (MQ=255)

                              gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGATCTTCTCACTAATCCAGCCGACGTTTGGTTTTCAGACACGt                                                                                                          >  2:5900678/1‑140 (MQ=255)

                               aaTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTc                                                                                                         >  2:1515840/1‑140 (MQ=255)

                                aTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                        >  1:995652/1‑140 (MQ=255)

                                    gCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGcc                                                                                                    >  1:849291/1‑140 (MQ=255)

                                    gCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGcc                                                                                                    >  1:2844078/1‑140 (MQ=255)

                                     cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                   <  1:1443328/140‑1 (MQ=255)

                                     cACGGGTAAAATCAGAATCGCGCGGGATTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                   <  1:2098100/140‑1 (MQ=255)

                                        ggttAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATa                                                                                                <  1:2365573/137‑1 (MQ=255)

                                         ggTAAAATCAGACTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGAGaa                                                                                               >  1:4761506/1‑140 (MQ=255)

                                         ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                               >  1:4646847/1‑140 (MQ=255)

                                         ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                               >  1:1088674/1‑140 (MQ=255)

                                         ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                               >  2:4870401/1‑140 (MQ=255)

                                           tAAAATCAGAATCGCGCGGGTTTGCGGTTCGAGCAGCAACGACTTCAACTCTATGCGGAGTAACGTGCATAAGAGATGAAACATGCTCTTCTCCATAATAAAGACGAAGATTTGTTTTCAGCCAGGTCTGGCCGATAAAc                                                                                             >  2:3231963/1‑140 (MQ=255)

                                             aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                           <  2:4335075/140‑1 (MQ=255)

                                                  aGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAAAATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGAGAAACATCAccg                                                                                      >  2:3913420/1‑140 (MQ=255)

                                                    aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATCAACATCAccgcc                                                                                    >  1:1165717/1‑140 (MQ=255)

                                                    aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCAccgcc                                                                                    >  1:3097520/1‑140 (MQ=255)

                                                        gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                                <  1:4734883/140‑1 (MQ=255)

                                                        gcgcgGGTTTGCGGTTCGCGCAGCAACGACAGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                                <  1:5212172/140‑1 (MQ=255)

                                                         cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGc                                                                               >  1:5487216/1‑140 (MQ=255)

                                                               tttGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTACTCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCaa                                                                         <  2:513718/140‑1 (MQ=255)

                                                                    ggTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACa                                                                    <  1:3173496/140‑1 (MQ=255)

                                                                     gTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAg                                                                   <  2:1944739/140‑1 (MQ=255)

                                                                       tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                 <  1:2491531/140‑1 (MQ=255)

                                                                       tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                 <  2:566155/140‑1 (MQ=255)

                                                                       tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                 <  1:1970514/140‑1 (MQ=255)

                                                                       tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                 <  2:3864577/140‑1 (MQ=255)

                                                                        cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                                >  1:448184/1‑140 (MQ=255)

                                                                         gcgcAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACg                                                               <  2:2560189/140‑1 (MQ=255)

                                                                           gcagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACCGCACGtt                                                             <  2:5010396/140‑1 (MQ=255)

                                                                           gcagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGtt                                                             <  1:5438935/140‑1 (MQ=255)

                                                                            cagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGttt                                                            <  1:5758213/140‑1 (MQ=255)

                                                                            cagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGttt                                                            <  2:1470927/140‑1 (MQ=255)

                                                                             atcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCTAAGCGATCAAAAACAGCACGTTTa                                                           <  2:3563239/138‑1 (MQ=255)

                                                                                aaCGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTacca                                                        >  1:3980203/1‑140 (MQ=255)

                                                                                 aCGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTaccac                                                       <  1:63061/140‑1 (MQ=255)

                                                                                    aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                    <  1:4033968/140‑1 (MQ=255)

                                                                                    aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                    <  1:5115104/140‑1 (MQ=255)

                                                                                    aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                    <  2:1640902/140‑1 (MQ=255)

                                                                                    aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTAAACACCAg                                                    >  2:6385857/1‑140 (MQ=255)

                                                                                     cTGCAACTCTTTGCGTATTACCGTCCATAAGCGATGAACCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAACCATCACCGCCGAAGCGATCAAAAACAGCACGTGTACCACCAGc                                                   <  2:5493670/140‑1 (MQ=255)

                                                                                     cTGCAACTCTTTGCGGATTACCGTCCATACGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCTCGTCTGGCCGATACTCATCACGGGCGATGCGATCAAAATCAGCACGTGTACCACCAGc                                                   <  2:4069775/140‑1 (MQ=255)

                                                                                     cTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGc                                                   <  2:2159504/140‑1 (MQ=255)

                                                                                         aaCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                               >  2:222880/1‑140 (MQ=255)

                                                                                         aaCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                               >  2:2649972/1‑140 (MQ=255)

                                                                                              tttGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGa                                          >  1:3641720/1‑140 (MQ=255)

                                                                                               ttGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGaa                                         >  2:1142362/1‑140 (MQ=255)

                                                                                                tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGact                                        >  2:2786570/1‑138 (MQ=255)

                                                                                                tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAat                                        >  2:661087/1‑140 (MQ=255)

                                                                                                tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAat                                        >  1:4345739/1‑140 (MQ=255)

                                                                                                  cGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCCCCACTGGAatat                                      >  2:5985791/1‑140 (MQ=255)

                                                                                                     aTTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTcc                                   <  2:4168820/140‑1 (MQ=255)

                                                                                                         aCGTCCATAAGCGATGAAACCTGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAACCATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGc                               <  2:509144/140‑1 (MQ=255)

                                                                                                          cGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCg                              <  2:1247861/140‑1 (MQ=255)

                                                                                                            tCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAg                            >  1:1851226/1‑140 (MQ=255)

                                                                                                              cATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGa                          >  1:5892489/1‑140 (MQ=255)

                                                                                                               aTAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGaa                         >  1:2155248/1‑140 (MQ=255)

                                                                                                               aTAAGCGATGAAACATGCTATTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGaa                         >  2:2430143/1‑140 (MQ=255)

                                                                                                                tAAGCGATGAAACATGCTCTTCTCCCTACTCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                        <  2:2696989/140‑1 (MQ=255)

                                                                                                                tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                        <  1:1317691/140‑1 (MQ=255)

                                                                                                                tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                        <  2:5472642/140‑1 (MQ=255)

                                                                                                                tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                        <  2:1274032/140‑1 (MQ=255)

                                                                                                                tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATAGTCCCGGCGAGGAAc                        <  1:4912997/140‑1 (MQ=255)

                                                                                                                 aaGCGATGAAACATGCTCTACTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACa                       <  1:751684/140‑1 (MQ=255)

                                                                                                                    cGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGc                    <  2:5521350/140‑1 (MQ=255)

                                                                                                                    cGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGc                    >  2:2113453/1‑140 (MQ=255)

                                                                                                                      aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt                  >  1:4947842/1‑140 (MQ=255)

                                                                                                                       tGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCCCCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCttt                 >  1:5814927/1‑140 (MQ=255)

                                                                                                                       tGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCttt                 >  1:4368303/1‑140 (MQ=255)

                                                                                                                        gAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTg                <  2:5197936/140‑1 (MQ=255)

                                                                                                                         aaaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGc               <  1:4113340/140‑1 (MQ=255)

                                                                                                                          aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAACACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa              <  1:599834/140‑1 (MQ=255)

                                                                                                                          aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa              <  1:389318/140‑1 (MQ=255)

                                                                                                                          aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa              <  2:6000127/140‑1 (MQ=255)

                                                                                                                          aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa              <  1:344800/140‑1 (MQ=255)

                                                                                                                          aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa              <  1:2252403/140‑1 (MQ=255)

                                                                                                                          aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa              <  1:5271269/140‑1 (MQ=255)

                                                                                                                          aaCATGCTCGTCTCCCTAATCCAGCCGCCGATTGGTTTTCAGCCACGTCAGGCCGAGAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa              <  1:3729218/140‑1 (MQ=255)

                                                                                                                              tGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGt          >  1:888917/1‑140 (MQ=255)

                                                                                                                                   ttctCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGa     >  2:4759976/1‑140 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTTGAATATTCCCGGCGAGGAACAGGCTTTGCAGTGTGCTGACg   >  2:1347741/1‑140 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACg   >  1:4237135/1‑140 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACg   >  1:5070881/1‑140 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACg   >  1:10411/1‑140 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGTNTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATNCCCGGCGNGGAACAGGCTTTGCAGGGTGCTGACg   >  1:5637427/1‑140 (MQ=255)

                                                                                                                                      tcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATCTTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGa  >  2:3327161/1‑140 (MQ=255)

GCGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACGA  >  NZ_CP025268/833089‑833362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`