BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,207,982	A→G	V51A (GTC→GCC)	xisE ←	excisionase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,207,982	0	A	G	99.8%	1490.5 / NA	429	V51A (GTC→GCC)	xisE	excisionase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (224/204); minor base T (1/0); total (225/204)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 429 total aligned reads displayed.

CCTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGT  >  NZ_CP025268/1207845‑1208118

ccTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCACAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCg                                                                                                                                        >  2:103876/1‑140 (MQ=255)

ccTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAATATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCg                                                                                                                                        >  2:3926119/1‑140 (MQ=255)

ccTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCg                                                                                                                                        <  1:5424177/140‑1 (MQ=255)

ccTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCg                                                                                                                                        <  1:6249530/140‑1 (MQ=255)

 ctactaTAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGt                                                                                                                                       >  2:1818707/1‑140 (MQ=255)

   actaTAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTcc                                                                                                                                     >  2:4890727/1‑140 (MQ=255)

       taAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCAc                                                                                                                                 >  1:4961483/1‑140 (MQ=255)

       taAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCAc                                                                                                                                 >  2:5356750/1‑140 (MQ=255)

       taAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGAGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCAc                                                                                                                                 >  2:2674956/1‑140 (MQ=255)

          aGCGGATATACGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAACAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  1:2257010/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTACTGAGTCATTTTTCTGGATTCCAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  2:2479732/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTCACTCGGCGTCCTCACGCa                                                                                                                              <  2:5597316/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  1:4445458/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  1:1079456/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  1:6392649/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  2:2057960/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  2:2752340/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  2:6445704/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  2:911867/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  1:1600981/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  1:3054866/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATATTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCTGCGTCCTCACGCa                                                                                                                              >  2:2278767/1‑140 (MQ=255)

           gCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCAc                                                                                                                             <  1:4649971/140‑1 (MQ=255)

            cGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACt                                                                                                                            >  1:3925858/1‑140 (MQ=255)

                     gTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAAc                                                                                                                   <  1:783156/140‑1 (MQ=255)

                      ttAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAAcc                                                                                                                  <  1:3029524/140‑1 (MQ=255)

                          ggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGcc                                                                                                              <  2:5971989/140‑1 (MQ=255)

                           gACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCt                                                                                                             <  2:2199367/140‑1 (MQ=255)

                              tGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGt                                                                                                          >  2:5410407/1‑140 (MQ=255)

                                aaaCATTATTCTTACGCGTTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGttt                                                                                                        >  2:3673924/1‑140 (MQ=255)

                                aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGttt                                                                                                        >  2:3841853/1‑140 (MQ=255)

                                aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTGGttt                                                                                                        >  2:595042/1‑140 (MQ=255)

                                 aaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTc                                                                                                       <  1:1861561/140‑1 (MQ=255)

                                   ccttattCTGACGCGGTCTTGCTGCCATTACTCAAAATCCGTTGCAAATGTATTGAGTCATTTTTCTGGATTACAGGTGTTACCAACTCCCCTACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCAt                                                                                                     <  2:1353393/138‑1 (MQ=255)

                                    attattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATg                                                                                                    >  2:1606776/1‑140 (MQ=255)

                                      tattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGc                                                                                                  >  1:1230247/1‑140 (MQ=255)

                                        ttCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCg                                                                                                <  1:349283/140‑1 (MQ=255)

                                           ttACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTg                                                                                             >  2:5846113/1‑140 (MQ=255)

                                              cgcgGTCTGTCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                          >  2:1405402/1‑140 (MQ=255)

                                              cgcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCACCTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                          <  2:804617/140‑1 (MQ=255)

                                                  gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaaa                                                                                      >  2:1714818/1‑140 (MQ=255)

                                                  gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaaa                                                                                      >  2:1975068/1‑140 (MQ=255)

                                                  gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaaa                                                                                      >  2:3166160/1‑140 (MQ=255)

                                                   tCTGGCTGCCAGTACTCAAAATCCGTTGCAATAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                     >  2:262794/1‑140 (MQ=255)

                                                       gCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTGTCATGGCCGGTGGACAaattaaat                                                                                 >  2:5693252/1‑140 (MQ=255)

                                                        cTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                                <  1:5972800/140‑1 (MQ=255)

                                                        cTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                                <  2:155094/140‑1 (MQ=255)

                                                        cTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                                <  1:1324397/140‑1 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           >  2:3406204/1‑140 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           >  2:2976475/1‑140 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           >  1:2865221/1‑140 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           >  2:4059511/1‑140 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGGTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATCAATTCTGcg                                                                           >  2:6230206/1‑139 (MQ=255)

                                                                 cTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGc                                                                       >  2:6339471/1‑140 (MQ=255)

                                                                     cactCCGGTGCCAAACTAATGAGGCATTGTTCGTGAGTACAGGTGTTACCAACTCCCCCACTACCTCGGCGTCCTCACGCACTCGCCAGAACCGGCCTTGGTTCATGGCCGGTGGACAAAATACAGTCTGCTTAGCagca                                                                   <  2:4293762/137‑4 (MQ=255)

                                                                     aaaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATaa                                                                   <  2:2115169/140‑1 (MQ=255)

                                                                      aaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAac                                                                  >  2:4474771/1‑140 (MQ=255)

                                                                        tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacga                                                                >  1:5774373/1‑140 (MQ=255)

                                                                        tCCGTTGCAAAAGTAATGAGTCAATTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacga                                                                <  2:6119895/140‑1 (MQ=255)

                                                                         ccGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                               <  1:500260/140‑1 (MQ=255)

                                                                          cGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                              <  1:3857114/140‑1 (MQ=255)

                                                                           gTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                             <  1:692608/140‑1 (MQ=255)

                                                                           gTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                             <  2:4685563/140‑1 (MQ=255)

                                                                           gTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                             <  1:5046893/140‑1 (MQ=255)

                                                                            ttGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCa                                                            >  2:5102141/1‑140 (MQ=255)

                                                                             tGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCaa                                                           <  1:1003262/140‑1 (MQ=255)

                                                                              gCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCACCTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAt                                                          <  1:5814660/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                     <  2:5251705/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                     <  1:6175465/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                     <  1:2317858/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACACGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGAGGa                                                     <  2:1161106/140‑1 (MQ=255)

                                                                                    gTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGac                                                    <  2:733629/140‑1 (MQ=255)

                                                                                     tAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGaca                                                   <  1:3594514/140‑1 (MQ=255)

                                                                                     tAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGaca                                                   <  2:4552053/140‑1 (MQ=255)

                                                                                     tAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGaca                                                   <  1:2063609/140‑1 (MQ=255)

                                                                                         gAGTCTTTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACt                                               <  1:3190726/140‑1 (MQ=255)

                                                                                         gAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACt                                               <  1:2522523/140‑1 (MQ=255)

                                                                                         gAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACt                                               >  2:3216588/1‑140 (MQ=255)

                                                                                         gAGTCATGTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAAGAAATTCTGCTTAGCATACCGACGCAATGTGGACACACt                                               <  1:5275231/140‑1 (MQ=255)

                                                                                            tCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                            <  1:4726766/140‑1 (MQ=255)

                                                                                                      tGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACtt                                  <  1:4528253/140‑1 (MQ=255)

                                                                                                               gtgtTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAtttt                         >  1:4178208/1‑140 (MQ=255)

                                                                                                                  ttACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTca                      >  2:658942/1‑140 (MQ=255)

                                                                                                                   tACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcag                     <  2:5556939/140‑1 (MQ=255)

                                                                                                                    aCCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                    >  1:868595/1‑140 (MQ=255)

                                                                                                                    aCCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                    >  1:3996721/1‑140 (MQ=255)

                                                                                                                    aCCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                    >  1:436749/1‑140 (MQ=255)

                                                                                                                       aaCTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCagcagc                 >  2:2414703/1‑140 (MQ=255)

                                                                                                                         cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAACTAAATACTGCTTAGCATATCGACGCAATGTGGACACACTTGGAGGAGTACTTCTGTATTTTACAGCAGccc               >  1:1458841/1‑140 (MQ=255)

                                                                                                                         cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGccc               >  1:1722783/1‑140 (MQ=255)

                                                                                                                          tCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa              <  1:1407887/140‑1 (MQ=255)

                                                                                                                          tCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa              <  2:3516167/140‑1 (MQ=255)

                                                                                                                            cccAACTAACTCGGCGTCCTCACTCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACGTCTGTATTTTTCAGCAGCCCaat            >  1:3747041/1‑138 (MQ=255)

                                                                                                                            cccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAtt            >  2:4756850/1‑140 (MQ=255)

                                                                                                                            cccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAtt            >  1:3697319/1‑140 (MQ=255)

                                                                                                                              cAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACTCTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttct          >  1:6064009/1‑140 (MQ=255)

                                                                                                                                actaactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttcttc        <  2:3550691/140‑1 (MQ=255)

                                                                                                                                 ctaactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCa       <  1:3268033/140‑1 (MQ=255)

                                                                                                                                    actCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga    >  1:562463/1‑140 (MQ=255)

                                                                                                                                    actCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga    >  1:4113198/1‑140 (MQ=255)

CCTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGT  >  NZ_CP025268/1207845‑1208118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`