BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,430,799	A→G	120 (TAA→TAG)	CXP41_RS07610 →	protein YdaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,430,799	0	A	G	99.8%	1600.9 / NA	458	120 (TAA→TAG)	CXP41_RS07610	protein YdaY
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (257/200); minor base T (0/1); total (257/201)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 458 total aligned reads displayed.

GCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAACTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTATTC  >  NZ_CP025268/1430662‑1430935

gCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCt                                                                                                                                         >  2:5227973/1‑140 (MQ=255)

gCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCt                                                                                                                                         >  2:281378/1‑140 (MQ=255)

   cTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGcc                                                                                                                                      <  2:423297/140‑1 (MQ=255)

        atatACGGCAGAACGAGGGATCGCGCTGCTTATGCAGAATCCTGAGTTGTACTGGCAGATCCGTGCAGAAGCAGCGAGAATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATATAAAAGCCATAGCTGCCCAGCg                                                                                                                                 <  2:911/140‑1 (MQ=255)

        agataCGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCg                                                                                                                                 <  1:6386630/138‑1 (MQ=255)

           taCGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCgg‑tg                                                                                                                             >  2:157049/1‑140 (MQ=255)

           taCGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCgg‑tg                                                                                                                             >  1:2211649/1‑140 (MQ=255)

                cAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTAt                                                                                                                        >  2:69362/1‑140 (MQ=255)

                cAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTAt                                                                                                                        >  1:707660/1‑140 (MQ=255)

                cAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTAt                                                                                                                        >  2:1385398/1‑140 (MQ=255)

                cAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTAt                                                                                                                        <  1:2916923/140‑1 (MQ=255)

                cAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTAt                                                                                                                        >  2:4763188/1‑140 (MQ=255)

                cAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTAt                                                                                                                        >  2:266570/1‑140 (MQ=255)

                 aGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATc                                                                                                                       >  2:3247028/1‑140 (MQ=255)

                  gAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCg                                                                                                                      <  1:370390/140‑1 (MQ=255)

                   aaCGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAANGCCATAGCTGCCCAGCGG‑TGGTTATCgg                                                                                                                     >  1:5663590/1‑140 (MQ=255)

                   aaCGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCgg                                                                                                                     >  1:1939726/1‑140 (MQ=255)

                   aaCGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCgg                                                                                                                     >  1:1043942/1‑140 (MQ=255)

                       cgggATCTCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCGTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATCGCTGCCCTGCGG‑TGGTTATCGGAGtt                                                                                                                 <  2:642126/139‑1 (MQ=255)

                         ggATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCg                                                                                                               <  1:5917511/140‑1 (MQ=255)

                         ggATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATACTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCg                                                                                                               <  2:4507083/140‑1 (MQ=255)

                             cgcgCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCggggg                                                                                                           >  2:815765/1‑140 (MQ=255)

                             cgcgCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCggggg                                                                                                           >  1:5412633/1‑140 (MQ=255)

                                              aTCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAgg                                                                                          >  1:862728/1‑140 (MQ=255)

                                              aTCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAgg                                                                                          >  2:3870054/1‑140 (MQ=255)

                                              aTCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAgg                                                                                          >  2:6273744/1‑140 (MQ=255)

                                              aTCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAgg                                                                                          >  1:2590065/1‑140 (MQ=255)

                                              aTCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAgg                                                                                          >  1:2119497/1‑140 (MQ=255)

                                              aTCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAgg                                                                                          >  1:343635/1‑140 (MQ=255)

                                              aTCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAgg                                                                                          >  1:2618235/1‑140 (MQ=255)

                                                         tACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCACGATGGAAg                                                                               >  1:6169365/1‑140 (MQ=255)

                                                         tACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAg                                                                               >  2:3586440/1‑140 (MQ=255)

                                                          aCTGGCAGATCCTTGCAGAAGCAGCCAGCCTCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGc                                                                              <  2:1637196/140‑1 (MQ=255)

                                                          aCTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGc                                                                              <  2:5043380/140‑1 (MQ=255)

                                                           cTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCg                                                                             >  1:1640372/1‑140 (MQ=255)

                                                             ggCAGATCCTTGCAGAAGCAGCCAGCATCGCCCATGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGC‑TGGTTATCGGAGTACGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCgag                                                                           <  2:5459275/140‑1 (MQ=255)

                                                                aGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGaaa                                                                        >  2:5103778/1‑140 (MQ=255)

                                                                   tCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAATGGCAAGATGGAAGCGAGAAAAAc                                                                     >  2:4926420/1‑140 (MQ=255)

                                                                   tCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGaaaatc                                                                     >  1:4647059/1‑138 (MQ=255)

                                                                   tCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAAc                                                                     >  1:5330475/1‑140 (MQ=255)

                                                                   tCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAAc                                                                     >  1:4085149/1‑140 (MQ=255)

                                                                   tCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTGATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGCAGCGAGAAAAAc                                                                     >  2:3972606/1‑140 (MQ=255)

                                                                    ccTCGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGCTAACAAAGCCATAGCTGCCCAGCGG‑TGGTTAGCGGAGTTCGGGGGAGCAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACt                                                                    <  2:5743684/140‑1 (MQ=255)

                                                                          aGACGCAGCCAGCATCGCCCAGGGTAAAGATCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAATATGGAAGCGAGAAAAACTCAGGtt                                                              <  2:1025693/140‑1 (MQ=255)

                                                                           gAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGCAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTg                                                             <  1:1972409/140‑1 (MQ=255)

                                                                            cagcagcCAGCATCGCCCAGGGTAAAGAGCAGCAGCAGCAGGATACGATAAAAAAGCCATAGCGGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGc                                                            <  1:5005970/139‑1 (MQ=255)

                                                                            aaGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGc                                                            >  1:3551432/1‑140 (MQ=255)

                                                                              gcagcCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGCGAAAGGGGGGAAAAGGCAAGATGGACGCGAGAAAAACTCAGGTTGCCa                                                          >  2:3036404/1‑140 (MQ=255)

                                                                              gcagcCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCa                                                          >  1:4199054/1‑140 (MQ=255)

                                                                              gcagaCAGCATCGCCCAGGGGAAAGAGCAGCAGAAGCAGGATACGCTAAAAAAGCCATAGCTGCCCAGCGG‑TGGTGATCGGAGTTCGGGGGAGAGAGGGGGGAAATGGCAAGATGGAAGCGAGAACGACTCAGGTGGccc                                                          >  2:3103715/1‑139 (MQ=255)

                                                                               cagccagcATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCAc                                                         <  1:181341/140‑1 (MQ=255)

                                                                                 gccagcATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCg                                                       >  2:2607483/1‑140 (MQ=255)

                                                                                  ccagcATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGa                                                      >  1:4961894/1‑140 (MQ=255)

                                                                                     gcATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATAc                                                   <  1:3900163/140‑1 (MQ=255)

                                                                                       aTCGCCCAGGTAAAAGAGCAGCAGAAGCAGGATACGATACAAAAGCCATAGCGGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCg                                                 <  2:3694968/140‑1 (MQ=255)

                                                                                       aTCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTGATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCg                                                 <  1:276110/140‑1 (MQ=255)

                                                                                         cGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGa                                               >  2:3194837/1‑140 (MQ=255)

                                                                                           cccAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAAc                                             <  1:1377330/140‑1 (MQ=255)

                                                                                           cccAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAAc                                             >  1:3078391/1‑140 (MQ=255)

                                                                                            ccAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAAcc                                            <  2:3715257/140‑1 (MQ=255)

                                                                                                     agagCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGa                                   >  1:5203634/1‑140 (MQ=255)

                                                                                                     agagCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGa                                   >  2:3422439/1‑140 (MQ=255)

                                                                                                       agcagcAGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGAcc                                 <  2:2211649/140‑1 (MQ=255)

                                                                                                       agcagcAGAAGCAGGATACGATAAAAAAGCCATAGCTGCACAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGAcc                                 <  2:2003040/140‑1 (MQ=255)

                                                                                                        gcagcaGAAGCAGGATACGATAAACAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGAccc                                <  1:5286215/140‑1 (MQ=255)

                                                                                                        gcagcaGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGAccc                                <  1:146535/140‑1 (MQ=255)

                                                                                                        gcagcaGAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGAccc                                <  1:4900744/140‑1 (MQ=255)

                                                                                                        cccccccaaGCAGGAACCGATAACCAAGCCAACGCAGCCCAGCGG‑GGGGTCTCGGAGTGCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGAccc                                <  2:5808658/133‑1 (MQ=255)

                                                                                                         catcagAAGCAGGATACTATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCg                               <  2:1708088/137‑1 (MQ=255)

                                                                                                         cagcagAAGCAGGATACGATAAACAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCg                               <  1:5133440/140‑1 (MQ=255)

                                                                                                         cagcagAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCg                               <  2:6051061/140‑1 (MQ=255)

                                                                                                         cagcagAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCg                               <  2:2881327/140‑1 (MQ=255)

                                                                                                          agcagAAGCAGGATACGATAAAACAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCgg                              <  1:3821964/140‑1 (MQ=255)

                                                                                                          agcagAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAATGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCgg                              >  2:2389359/1‑140 (MQ=255)

                                                                                                          agcagAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCACGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCgg                              >  2:4039997/1‑140 (MQ=255)

                                                                                                          agcagAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCgg                              >  1:2410667/1‑140 (MQ=255)

                                                                                                          agcagAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCgg                              >  2:6341820/1‑140 (MQ=255)

                                                                                                          agcagAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCgg                              <  1:548781/140‑1 (MQ=255)

                                                                                                          agcagAAGCAGGATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCgg                              >  1:6070877/1‑140 (MQ=255)

                                                                                                                   aGGATACGATACAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAgg                     <  1:123587/140‑1 (MQ=255)

                                                                                                                     gATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAg                   <  1:5727781/140‑1 (MQ=255)

                                                                                                                     gATACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAg                   <  1:3314799/140‑1 (MQ=255)

                                                                                                                      agaCGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGCACGGGGGGAAATGGCAAGATGGAAGCGAGAACAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAgt                  >  2:3350084/3‑140 (MQ=255)

                                                                                                                      aTACGATAAAAAAGCCATATCTGCCCTGCGG‑TGGTTATCGTAGTTCGGGGGAGAAAGGGGTGAAAAGCCAAGATGGTAGCGAGAAATACTCAGGTTGCCACCGATACCTGAACCAGAAATATACCCGGTGCTTAAGGAgt                  <  2:1299085/140‑1 (MQ=255)

                                                                                                                      aTACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAgt                  <  1:626948/140‑1 (MQ=255)

                                                                                                                      aTACGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAgt                  >  2:1464135/1‑140 (MQ=255)

                                                                                                                      aTAAGATAATAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGTGAAAAACGCAGGGTGCCACCGTTACCGGATCCAGAAATCGACCCGGGGCTTATGGAgg                  >  2:6143453/1‑139 (MQ=255)

                                                                                                                         cGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAgttgt               >  2:2286307/1‑140 (MQ=255)

                                                                                                                         cGATAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAgttgt               >  1:5853469/1‑140 (MQ=255)

                                                                                                                            tAAAAAAGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGGtgttgt            >  1:3594585/1‑140 (MQ=255)

                                                                                                                               aaaaGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACg         >  1:1521339/1‑140 (MQ=255)

                                                                                                                                aaaGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGc        <  1:2859786/140‑1 (MQ=255)

                                                                                                                                aaaGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGc        <  1:2695532/140‑1 (MQ=255)

                                                                                                                                 aaGCCATAGCTGCCCAGCGGTTGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGc        <  2:4584040/140‑1 (MQ=255)

                                                                                                                                 aaGCCATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGcc       <  2:4430395/140‑1 (MQ=255)

                                                                                                                                     cATAGCTGCCCAGCGG‑TGGTTATCGGCGTTCGGGGGAGCAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTAtt   <  1:4566156/140‑1 (MQ=255)

                                                                                                                                     cATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTAtt   <  2:5883235/140‑1 (MQ=255)

                                                                                                                                     cATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTAtt   <  1:3269649/140‑1 (MQ=255)

                                                                                                                                     cATAGCTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTAtt   <  1:3766622/140‑1 (MQ=255)

                                                                                                                                     cATAGCTGCCCAGCGG‑TGGTTAGCGGAGATCGGGGGATAAAGGGGGGAAAAGTCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTAtt   <  2:5620349/140‑1 (MQ=255)

GCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAACTGCCCAGCGG‑TGGTTATCGGAGTTCGGGGGAGAAAGGGGGGAAAAGGCAAGATGGAAGCGAGAAAAACTCAGGTTGCCACCGATACCGGAACCAGAAATAGACCCGGTGCTTAAGGAGTTGTTGTACGCCTATTC  >  NZ_CP025268/1430662‑1430935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`