BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,496,208	G→A	R163K (AGG→AAG)	cybB →	cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,496,208	0	G	A	98.5%	934.8 / NA	273	R163K (AGG→AAG)	cybB	cytochrome b561
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (63/203); minor base T (0/4); total (63/210)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

ACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTATTTG  >  NZ_CP025268/1496072‑1496344

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCTGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                       <  1:3016581/140‑1 (MQ=255)

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                       <  2:2110400/140‑1 (MQ=255)

   aTGCCTTACGCTTCAGAGGCCAATTTCGACCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaa                                                                                                                                    <  1:2704673/140‑1 (MQ=255)

    tGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaac                                                                                                                                   >  1:5948525/1‑140 (MQ=255)

     gCCTTACTCTTCATAGGCCAAGTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCTAATCTGGGAGATTTTGTCATCGGTTTGCACGCAGCGGCTGCACAGGCACACCACTATATCTGGAAGGacaaca                                                                                                                                  <  2:4679683/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  1:1578043/140‑1 (MQ=255)

      ccTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGCCTCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacac                                                                                                                                 >  2:2763813/1‑140 (MQ=255)

      ccTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacac                                                                                                                                 >  2:5443939/1‑140 (MQ=255)

       cTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacaca                                                                                                                                <  2:5984935/140‑1 (MQ=255)

          aCGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACtt                                                                                                                             <  2:4734463/140‑1 (MQ=255)

            gCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCt                                                                                                                           <  1:670694/140‑1 (MQ=255)

                cAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGc                                                                                                                       >  1:538523/1‑140 (MQ=255)

                 cgacgCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGACTCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCa                                                                                                                      <  2:1271024/136‑1 (MQ=255)

                 agagGCCAATTTCGAACGGGTAGAGAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCa                                                                                                                      >  1:397059/1‑140 (MQ=255)

                    ggCCAATTTCGAACGGGTAGATAGCTAAACGTCGTGTCAGGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCGGCACTGGCACACCACTATTTCTGGCAGGACAACACACTACTACGCatga                                                                                                                   <  2:5497796/140‑1 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                                 <  2:3141857/140‑1 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                                 <  2:826187/140‑1 (MQ=255)

                       cAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGc                                                                                                                <  2:3020626/140‑1 (MQ=255)

                        aaTTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCGCGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGcc                                                                                                               >  1:4038972/1‑140 (MQ=255)

                          tgtCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCcgc                                                                                                             <  2:227234/138‑1 (MQ=255)

                             cGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTa                                                                                                          <  2:2142146/140‑1 (MQ=255)

                             cGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTa                                                                                                          <  2:1798217/140‑1 (MQ=255)

                             cGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTa                                                                                                          <  1:1098267/140‑1 (MQ=255)

                               aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        <  1:4228121/140‑1 (MQ=255)

                               aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        <  2:5032545/140‑1 (MQ=255)

                                aCGGGTAGATAGCAGAATGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGACCTCGGGTTGCACGCTGCGGCGGCACGGGCACCCCACTATTGCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAAc                                                                                                       <  2:4851782/140‑1 (MQ=255)

                                 cGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACg                                                                                                      <  2:1846061/140‑1 (MQ=255)

                                 cGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACg                                                                                                      <  1:4994583/140‑1 (MQ=255)

                                 cGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACGTCTACGCATGATGCCGCGTAAACg                                                                                                      <  2:4792451/140‑1 (MQ=255)

                                      aGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCt                                                                                                 <  1:4598489/140‑1 (MQ=255)

                                          aGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAg                                                                                             >  2:610983/1‑140 (MQ=255)

                                            cTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGa                                                                                           >  2:5245668/1‑140 (MQ=255)

                                              tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGata                                                                                         >  2:2193723/1‑140 (MQ=255)

                                                 aGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATAttt                                                                                      >  2:2545904/1‑140 (MQ=255)

                                                  gTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTa                                                                                     <  1:583515/140‑1 (MQ=255)

                                                      tGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGa                                                                                 >  1:5458922/1‑140 (MQ=255)

                                                       ggCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGaa                                                                                <  2:4409304/140‑1 (MQ=255)

                                                       ggCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGaa                                                                                >  1:3722185/1‑140 (MQ=255)

                                                             aaaCGCTGGCTAATGTGGGATTTTTTGTATTGGGGTTGCACGCTGCGGCTTCACTGGCACACCACTATTTATGGAAGGAAAACAAAATTCTACGCATTATGCCGCTTAAACTTTCCTGATGGATTTTTAAACAAAACGcc                                                                          <  1:3006122/140‑1 (MQ=255)

                                                             aaaCGCTGGCTAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:6023974/140‑1 (MQ=255)

                                                             aaaCGCTGGCTAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCAATATTTCTGGAAGTACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTTAAGGATATTTAAAGAAAACGcc                                                                          <  1:2158091/140‑1 (MQ=255)

                                                             aaaCGCTGGCGACTCTGGGATATTTGGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:5660401/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGTATTTTTTGTCATCGGGTTGCACGCTGCGGCTGCACGGGCACACCTCTATTTCTGTATGGACAACACACTTCTAGGCTTGATGCCGCTTAAACTTTCCTGAAGTATATTTAATGAAACCGcc                                                                          <  1:2980408/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCTGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGTAAGGACAACACACTTATACGCATGATGCCGCGTAAACTTTCCTTAAGGATATTTAAAGAAAACGcc                                                                          <  1:4544199/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGTCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:5759176/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:3213195/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:4352216/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          >  1:3779172/1‑140 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:348284/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:5659966/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAAACTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTTCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTAAGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:4951233/140‑1 (MQ=255)

                                                                  cTGGCGAATCTGGGTTATTTTTTCATGGGGTTGCAGGCTGCGGCTGCACTGGCACACCAATATTTCTGGAAGGACAACACACTTATACGATTGATGCGGCTTAAACGTTCCTGATGTTTTTTTAAAGAAAACGCCTGTAc                                                                     <  1:2562086/140‑1 (MQ=255)

                                                                  cTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTAc                                                                     <  2:168608/140‑1 (MQ=255)

                                                                    ggCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGATGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGACGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTa                                                                   <  2:461841/140‑1 (MQ=255)

                                                                        aaTCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCTTGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAAc                                                               <  1:4255666/140‑1 (MQ=255)

                                                                          tCTGGTATATTTTGTCATGGGGTTGCACGCTGCGGCTGCACTGGCACACAACTTTTTCTGGAAGGACAACACACTTCTACGCATTATGCCGCTTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCg                                                             <  1:2822559/140‑1 (MQ=255)

                                                                          tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                             <  2:2177686/140‑1 (MQ=255)

                                                                          tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                             <  2:1724908/140‑1 (MQ=255)

                                                                            tGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGAGGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAc                                                           <  1:1251833/140‑1 (MQ=255)

                                                                             gggATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAACACCGAcc                                                          <  2:5477047/140‑1 (MQ=255)

                                                                             gggATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc                                                          <  2:3722185/140‑1 (MQ=255)

                                                                               gttatTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGTAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGTTATTTAAAGAAAACGCCTGTACTAAAACCGACCCg                                                        <  1:6385810/138‑1 (MQ=255)

                                                                               gATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCg                                                        <  2:341760/140‑1 (MQ=255)

                                                                                atatTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGt                                                       <  1:5247016/140‑1 (MQ=255)

                                                                                    tttGTCATGGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGNGTAAACGTTCCTTCAGTATATTTAAAGAAAACGCCTGTACTAACACCGANCCGTGGTa                                                   <  1:5713809/140‑1 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  1:3545821/140‑1 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGAACTGGAACACCAATTTTTCTGGAAGGACAACACACTTATACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  1:3270221/140‑1 (MQ=255)

                                                                                    gctGTCATCGGGGTGCACGCGGCGGCGGCACTGGCACACCACTATTTCTGGAAGGACAACACACTACTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGtc                                                   <  2:1509700/138‑2 (MQ=255)

                                                                                      tGTCATCGGGTTGCGCGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACa                                                 <  2:4038972/140‑1 (MQ=255)

                                                                                       gTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCAATATTTCTGTAAGGACAACACACTTCTACGCATGATGCCGCTTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAg                                                <  1:146915/140‑1 (MQ=255)

                                                                                         cATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGc                                              >  1:1045571/1‑140 (MQ=255)

                                                                                         cATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGc                                              <  1:5063315/140‑1 (MQ=255)

                                                                                          aTCGGGTTGCACGCTGCGGCTGCACTTTCAAACAACTATTTCTGTAAGGACAACAAACTTATACGCATTATGCCGAGTAAACTTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGTTACAGGct                                             <  2:2059141/140‑2 (MQ=255)

                                                                                          aTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                             <  2:3262923/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:4459100/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:2519077/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:3183810/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  2:5006931/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCAAACCACTATTTCTGGAAGTACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAAACGACCCGTGGTACAGGCGAAGAATa                                      <  2:2643715/140‑1 (MQ=255)

                                                                                                 tGCACGCGGCGGCTGAACGGGCACACCACTATTTCTGGACGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGTTATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:1906804/140‑1 (MQ=255)

                                                                                                       cTGGGGCTGCACTGGCACACCACTTTTTCTGGATGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGTTTATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  1:2334594/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTTTTTATGGAAGGACAACACACTTCTACGCATGTTGCCGCGTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  1:4964317/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  2:2016942/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGAGGCCGCGTAAACGTTCCTGATGGATATTTAAATAACACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  1:4317223/140‑1 (MQ=255)

                                                                                                         gCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTa                              >  2:3184151/1‑140 (MQ=255)

                                                                                                                cACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGa                       <  2:5169568/140‑1 (MQ=255)

                                                                                                                 aCTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGATCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGaa                      <  2:6059558/140‑1 (MQ=255)

                                                                                                                  cTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAg                     >  2:4520739/1‑140 (MQ=255)

                                                                                                                  cTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAg                     <  1:5595635/140‑1 (MQ=255)

                                                                                                                  cTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAg                     <  1:4726470/140‑1 (MQ=255)

                                                                                                                   tGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgc                    <  1:2291255/140‑1 (MQ=255)

                                                                                                                     gCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc                  <  1:6163534/140‑1 (MQ=255)

                                                                                                                     gCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc                  <  2:397059/140‑1 (MQ=255)

                                                                                                                     gCACACCACTATTACTGGAAGGACAACACACTACTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTGAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc                  <  2:6207647/140‑1 (MQ=255)

                                                                                                                          ccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGACGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCtatt             <  1:966488/140‑1 (MQ=255)

                                                                                                                          ccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCtatt             >  2:1253860/1‑140 (MQ=255)

                                                                                                                                tttCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTAtattt       >  1:4833896/1‑140 (MQ=255)

                                                                                                                                tttCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTAtattt       >  2:5173947/1‑140 (MQ=255)

                                                                                                                                  tCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTAtatttat     <  2:2933483/140‑1 (MQ=255)

                                                                                                                                     ggAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTATTTg  >  2:5310247/1‑140 (MQ=255)

ACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTATTTG  >  NZ_CP025268/1496072‑1496344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`