BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,280,334	A→G	342 (TAA→TAG)	yejE →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,280,334	0	A	G	99.7%	1075.4 / NA	310	342 (TAA→TAG)	yejE	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (165/143); minor base T (0/1); total (165/145)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

CTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGT  >  NZ_CP025268/2280197‑2280470

   ttACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCTATATTATTGTCTTTGCTGAGCGTTATTGGTGAAGCCGTCCTCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGa                                                                                                                                     >  2:1666722/1‑140 (MQ=255)

     aCAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACg                                                                                                                                   <  1:4496787/140‑1 (MQ=255)

       aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                 <  1:35357/140‑1 (MQ=255)

       aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                 <  1:1319183/140‑1 (MQ=255)

       aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                 <  2:113439/140‑1 (MQ=255)

        aGGGAAATATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGACCGCGACGCATTTGATCCTCATAAGGCGGTGTAGCATGACGCaa                                                                                                                                >  2:463053/1‑140 (MQ=255)

        aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCaa                                                                                                                                >  2:4005284/1‑140 (MQ=255)

         gggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCaaa                                                                                                                               <  2:5696779/140‑1 (MQ=255)

          ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                                                                                                                              >  1:2815847/1‑140 (MQ=255)

          ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                                                                                                                              >  2:1459914/1‑140 (MQ=255)

          ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                                                                                                                              >  1:3927212/1‑140 (MQ=255)

          ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAc                                                                                                                              >  1:1412320/1‑140 (MQ=255)

           gAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTAGTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAct                                                                                                                             <  2:3665737/140‑1 (MQ=255)

             aaaaTAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGAGATAGTATTGTCTTTGCTGTGCTGTATTGGTGTAGCCGTCCGCGACGCATTTGATCCTATTTAGGCGGTGTAGCATGACGCAAActct                                                                                                                           >  2:3016603/1‑140 (MQ=255)

                   aCCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGc                                                                                                                     >  1:874894/1‑140 (MQ=255)

                    ccTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCg                                                                                                                    >  1:2221763/1‑140 (MQ=255)

                    ccTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCg                                                                                                                    >  1:2765897/1‑140 (MQ=255)

                    ccTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTGTATTGGTGAAGCCGTCCGCGACGCATTTGATCCGAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCg                                                                                                                    >  1:5244217/1‑140 (MQ=255)

                        cAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTg                                                                                                                >  2:6301776/1‑140 (MQ=255)

                           gCCCCGTGGCTTGGGATCACCGCCTGCGTGTCGGTGGCGAGCTTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGaaa                                                                                                             >  2:2514513/1‑140 (MQ=255)

                               cGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAAttt                                                                                                         >  2:3333664/1‑140 (MQ=255)

                               cGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATAGTCTTGTCTTTTCTGATCTTTATTGGTGAAGCCGTCCGCGACGCAGTTGATCCTAATAAGGCGGTGGAGCATGACGCCAACTCTGTGAGCGATTGaaacgtg                                                                                                         >  1:1177976/1‑136 (MQ=255)

                                     ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                   <  1:2964791/140‑1 (MQ=255)

                                     ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                   <  1:6301777/140‑1 (MQ=255)

                                     ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                   >  1:3399731/1‑140 (MQ=255)

                                     ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                   <  1:1429556/140‑1 (MQ=255)

                                          aTCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGtt                                                                                              <  1:4808162/140‑1 (MQ=255)

                                              ccGCCTTCTTGTCGGTGGCGNTATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTNTGTTAGCNATTGAAAATTTGTCGGTGGGTTTTCg                                                                                          >  1:5630973/1‑140 (MQ=255)

                                               cGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTAGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTCGCGATTGTCACTTTGTCGGTGGGTTTTCGc                                                                                         >  2:6013940/1‑140 (MQ=255)

                                                 ccTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCa                                                                                       <  1:5722217/140‑1 (MQ=255)

                                                 ccTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCa                                                                                       <  2:4091633/140‑1 (MQ=255)

                                                 ccTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCa                                                                                       <  1:3639016/140‑1 (MQ=255)

                                                 ccTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCa                                                                                       <  2:2776658/140‑1 (MQ=255)

                                                      ttGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGc                                                                                  <  1:4435862/140‑1 (MQ=255)

                                                      gtGTCGGTGGCGATAATTTTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGATAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGc                                                                                  <  2:200878/139‑1 (MQ=255)

                                                       tGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCa                                                                                 <  1:1039191/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGCTCTTTATTGGAGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  2:2153186/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTACTAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  2:4763981/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  1:1459914/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  2:3622405/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  1:1862826/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  2:1229111/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                                <  2:3900574/140‑1 (MQ=255)

                                                        gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTCAGCGATTGAAAATTTGTCGGTGGTTTTTCGCCATCAGCaa                                                                                <  1:3054987/140‑1 (MQ=255)

                                                          cGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                              <  1:1527147/140‑1 (MQ=255)

                                                           ggTGGCGATATTATTGTCTTTGCTGATCTGTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATACGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATGGAAATTTTGGCGGTGGGGGTTCGCCATCCGCAAAcc                                                                             >  2:560528/1‑140 (MQ=255)

                                                           ggTGGCGATATTAGTGTCTTTGCTGATCTTGATTGGTGAAGCCGTCCGCGACGCATTGGATCCTCATAAGGCGGTGTAGCAGGACGCAATCTCTGTTAGCGAGTGCAAATTGGTCGGGGGGTTTTCGCCATCAGCAAAcc                                                                             >  2:1058305/1‑140 (MQ=255)

                                                             tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                           <  1:6397566/140‑1 (MQ=255)

                                                               gCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtac                                                                         <  1:203903/140‑1 (MQ=255)

                                                                cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacg                                                                        <  1:6186724/140‑1 (MQ=255)

                                                                  atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                      <  2:4939573/140‑1 (MQ=255)

                                                                  atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                      <  1:3843227/140‑1 (MQ=255)

                                                                  atatTAGTGTCTTTGCTGTTCTTTATTGGTGAAGCCGTCCGCGACGCTTTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGGTAGCGATTGATAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                      <  2:3549224/140‑1 (MQ=255)

                                                                  atatTAGTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                      <  2:2425157/140‑1 (MQ=255)

                                                                   tattatTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCgtacgtac                                                                     >  1:5999771/1‑140 (MQ=255)

                                                                    attattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa                                                                    <  1:1378361/140‑1 (MQ=255)

                                                                    attattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa                                                                    <  2:90220/140‑1 (MQ=255)

                                                                      tattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGTCGCTTTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCATCAAACCGTACGTACagt                                                                  <  2:5147663/140‑1 (MQ=255)

                                                                      tattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACagt                                                                  <  1:1104750/140‑1 (MQ=255)

                                                                       attGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGTGTTTTCGCCATCAGCAAACCGTACGTACagta                                                                 >  1:1663539/1‑140 (MQ=255)

                                                                           tCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCa                                                             >  2:6422456/1‑140 (MQ=255)

                                                                             tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAat                                                           >  2:1812643/1‑140 (MQ=255)

                                                                             tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAat                                                           >  1:4363426/1‑140 (MQ=255)

                                                                              ttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatg                                                          >  2:2956642/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATCAGGCGGTGTAGCATGACGCTAACTCTGTTAGCGATTGACAATTTGACGGTGGGATTTCGAGATCAGCAAACCGTACGTACAGTAGTCAatgt                                                         >  2:6153945/1‑139 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                 <  1:3240502/68‑1 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  2:4185099/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  2:4135029/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  2:6239543/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  1:1059579/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  2:1933213/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  1:970939/1‑140 (MQ=255)

                                                                               tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  1:5544437/1‑140 (MQ=255)

                                                                                    aTCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGttt                                                    <  2:5556552/140‑1 (MQ=255)

                                                                                         tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                               >  1:5421582/1‑140 (MQ=255)

                                                                                                gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTg                                        >  2:5356081/1‑140 (MQ=255)

                                                                                                 aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGa                                       >  1:785255/1‑140 (MQ=255)

                                                                                                 aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGa                                       >  1:722917/1‑140 (MQ=255)

                                                                                                   gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAgg                                     >  2:3169433/1‑140 (MQ=255)

                                                                                                   gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAgg                                     <  2:1382050/140‑1 (MQ=255)

                                                                                                    ccGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGc                                    <  1:5282839/140‑1 (MQ=255)

                                                                                                         cgcgACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCg                               >  1:4106829/1‑140 (MQ=255)

                                                                                                         cgcgACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCg                               >  1:4903532/1‑140 (MQ=255)

                                                                                                              cGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcg                          >  1:1804335/1‑140 (MQ=255)

                                                                                                                  tttGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctgg                      <  1:2489595/140‑1 (MQ=255)

                                                                                                                        ccTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggcgctgg                >  2:5083585/1‑140 (MQ=255)

                                                                                                                           aataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg             >  1:6130137/1‑140 (MQ=255)

                                                                                                                           aataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg             >  1:3339478/1‑140 (MQ=255)

                                                                                                                             taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt           <  1:5072129/140‑1 (MQ=255)

                                                                                                                             taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt           >  1:2372061/1‑140 (MQ=255)

                                                                                                                             taaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtgggt           <  2:220603/140‑1 (MQ=255)

                                                                                                                                  cGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTc      >  2:4391802/1‑140 (MQ=255)

                                                                                                                                    gtgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACt                                                >  2:5324586/1‑96 (MQ=255)

                                                                                                                                    gtgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAg    >  2:3774269/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                         >  1:2701985/1‑86 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCNAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  1:5662660/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  2:4833888/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  2:4332380/1‑140 (MQ=255)

                                                                                                                                     tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg   >  2:5823384/1‑140 (MQ=255)

                                                                                                                                      gtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGt  <  1:2112132/140‑1 (MQ=255)

CTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGT  >  NZ_CP025268/2280197‑2280470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`