BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,415,086	G→C	T101S (ACC→AGC)	yfbS ←	SLC13 family permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,415,086	0	G	C	99.5%	1471.4 / NA	425	T101S (ACC→AGC)	yfbS	SLC13 family permease
Reads supporting (aligned to +/- strand): ref base G (2/0); new base C (200/222); total (202/223)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 425 total aligned reads displayed.

TAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414949‑2415222

    cAGCCCGGCAAAACGTCACGGCATCATCAGACGTGACCGCGACGTTTGCAAACGCATCGCCACGCTTCACACCACGGGAATAAAGAAAGCGACACCGCCGGTTGACCTCATAAACGCGCCAAGCCCCGCGACGCTCCGCa                                                                                                                                    <  2:3530734/140‑1 (MQ=255)

     aGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCAt                                                                                                                                   >  1:380780/1‑140 (MQ=255)

     aGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCAt                                                                                                                                   >  2:5994460/1‑140 (MQ=255)

       cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:5430783/1‑140 (MQ=255)

            cAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACaaa                                                                                                                            >  2:5559263/1‑140 (MQ=255)

               aaCTTATCGGCATCATCAGACGTGACGGCGACTTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCTACAACGCCGGTTGAAATCATAAACGCTCCAAGCCCCGCGACGCTCAGCATCTACATAAcc                                                                                                                         <  2:125384/140‑1 (MQ=255)

               aaCTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAAcc                                                                                                                         <  1:4646149/140‑1 (MQ=255)

               aaCTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAAcc                                                                                                                         <  2:202383/140‑1 (MQ=255)

                aCTTAACGGCATCATCAGAAGTGACGGCGACGTTGGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACACCGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCa                                                                                                                        <  2:41797/140‑1 (MQ=255)

                          atcatcAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGTAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGAt                                                                                                              <  1:3330303/140‑1 (MQ=255)

                           tcatcaGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGAtt                                                                                                             >  1:710913/1‑140 (MQ=255)

                           tcatcaGACGTGACGGCGACGTTTGCATACGCATGGAAACGATTAACACCACGGGACTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGAtt                                                                                                             >  2:2081086/1‑140 (MQ=255)

                                   cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  2:378014/1‑140 (MQ=255)

                                     tGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGc                                                                                                   >  2:2970018/1‑140 (MQ=255)

                                     tGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGc                                                                                                   >  2:2900470/1‑140 (MQ=255)

                                       aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 >  2:4608046/1‑140 (MQ=255)

                                       aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 >  2:608668/1‑140 (MQ=255)

                                       aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGGTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATGTTGATTTCACTATTGccc                                                                                                 >  1:4439822/1‑140 (MQ=255)

                                        cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  2:5081102/140‑1 (MQ=255)

                                        cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  1:1977300/140‑1 (MQ=255)

                                            gACGTTTGCATACGCATGGCAACGCTNAACACCAGGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCANTATTGCCCGCAAc                                                                                            <  1:5640057/140‑1 (MQ=255)

                                                       aCGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGcc                                                                                 <  2:3040134/140‑1 (MQ=255)

                                                       aCGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGcc                                                                                 <  2:4880600/140‑1 (MQ=255)

                                                       aCGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGGTGACCTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCCGcc                                                                                 >  1:659962/1‑140 (MQ=255)

                                                         gCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCAt                                                                               <  1:3057763/140‑1 (MQ=255)

                                                         gCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCAt                                                                               >  1:1537002/1‑140 (MQ=255)

                                                         cccaggCACCGCCTAACACCACGCGCCTAAAGCTAGCGCCACCGCCGGGAGACCGCATCACCGCGCCAAGCCCCGCGACGCTCCGCATCCACCAACCCCCCCCCTTGATTTCACGCGAGCCCGCACCAATGACCAGccca                                                                               <  2:5838419/136‑3 (MQ=255)

                                                          cATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATg                                                                              <  1:4306192/140‑1 (MQ=255)

                                                          cATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATg                                                                              <  2:4929640/140‑1 (MQ=255)

                                                          cATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATg                                                                              <  1:5144527/140‑1 (MQ=255)

                                                            tGGCAACGCTTAACACCACGGGAATAAAGATAGAGACAACGCCGGTTGAAATCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGtt                                                                            <  2:3588359/140‑1 (MQ=255)

                                                              gCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTcc                                                                          >  1:4594273/1‑140 (MQ=255)

                                                                  cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                      >  1:4961851/1‑140 (MQ=255)

                                                                  cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                      <  1:3865433/140‑1 (MQ=255)

                                                                  cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                      <  1:5869367/140‑1 (MQ=255)

                                                                   gCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTa                                                                     >  2:636919/1‑140 (MQ=255)

                                                                    cTTAACACCACGGGACTAAAGATAGCGACAACGCCGGTTGAACTCAGAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATAGTGAGTTCACTATTGCCCGCAACGTTGACCAGCCATGTTCCCATTAc                                                                    <  2:1180555/140‑1 (MQ=255)

                                                                    cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                    <  2:2844197/140‑1 (MQ=255)

                                                                     ttAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACg                                                                   <  1:1191349/140‑1 (MQ=255)

                                                                          accacTGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:452119/140‑1 (MQ=255)

                                                                          accacGTGAATAAAGATAGCGACAACGCCTGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATATTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:3861182/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:895699/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:69540/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  1:2841885/140‑1 (MQ=255)

                                                                          accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  1:424995/140‑1 (MQ=255)

                                                                            cacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  1:1886246/1‑140 (MQ=255)

                                                                            cacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  1:209090/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAATCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  2:3288755/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCTac                                                           >  1:5509673/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  1:3998695/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  1:6055428/1‑140 (MQ=255)

                                                                             acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  2:3112156/1‑140 (MQ=255)

                                                                              cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:5072524/1‑140 (MQ=255)

                                                                              cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:6295152/1‑140 (MQ=255)

                                                                               gggAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAacac                                                         >  2:1072422/1‑140 (MQ=255)

                                                                               gggAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAacac                                                         <  1:1453958/140‑1 (MQ=255)

                                                                               gggAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAacac                                                         >  1:895579/1‑140 (MQ=255)

                                                                                   aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     <  1:785179/140‑1 (MQ=255)

                                                                                   aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     <  1:1785191/140‑1 (MQ=255)

                                                                                    taccgATCGCGCCATCGCCGGATGAACGCATACCCGCGCCAAGCCCCGCGCCGCGCAGCATCAACAAACCCACCACTCGGAGTTCACTCTTGCCCGCAACATAGACCCGCCATGTTCCCATTACGGTGGCAACCCCggaa                                                    <  2:730222/136‑3 (MQ=255)

                                                                                    tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  1:4707527/140‑1 (MQ=255)

                                                                                    tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  2:1904805/140‑1 (MQ=255)

                                                                                    tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  1:2987288/140‑1 (MQ=255)

                                                                                      aaGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTCTTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACg                                                  >  1:4813544/1‑140 (MQ=255)

                                                                                      aaGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCCGCCATGTTCCCATTACGGTGGCAACACCGGTACg                                                  <  2:5591776/140‑1 (MQ=255)

                                                                                         aTAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGAc                                               >  2:5700760/1‑140 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCACCACCGGTACGGACCa                                             >  2:2538930/1‑140 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  1:242040/133‑1 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             >  1:3282458/1‑140 (MQ=255)

                                                                                           aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                             >  2:2395645/1‑140 (MQ=255)

                                                                                            gCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaa                                            >  1:2617731/1‑140 (MQ=255)

                                                                                             cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                           >  2:5132702/1‑140 (MQ=255)

                                                                                                cAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCAGCAACAAAACCAACATTTTGATTTCACTATTGCCCGCACCTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGCCCAAACCa                                        >  2:3442886/1‑140 (MQ=255)

                                                                                                 aaCGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCAt                                       >  1:2755273/1‑140 (MQ=255)

                                                                                                   cGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTCATGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCg                                     >  2:4693030/1‑140 (MQ=255)

                                                                                                   cGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCg                                     >  1:1603376/1‑140 (MQ=255)

                                                                                                    gCCGTTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:3361526/140‑1 (MQ=255)

                                                                                                    gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  1:2852293/140‑1 (MQ=255)

                                                                                                     ccGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGcc                                   <  1:2210232/140‑1 (MQ=255)

                                                                                                      cGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCa                                  >  2:2309235/1‑140 (MQ=255)

                                                                                                       ggTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaa                                 >  2:1498720/1‑140 (MQ=255)

                                                                                                             aCTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAAtaataa                           >  2:4381652/1‑140 (MQ=255)

                                                                                                                   aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATATTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAgg                     <  2:3177578/140‑1 (MQ=255)

                                                                                                                     aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  2:3981977/140‑1 (MQ=255)

                                                                                                                     aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGGTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  2:2207175/140‑1 (MQ=255)

                                                                                                                        cgcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCa                >  2:1815828/1‑140 (MQ=255)

                                                                                                                         gcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCaa               >  2:534463/1‑140 (MQ=255)

                                                                                                                         gcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCaa               >  1:3413975/1‑140 (MQ=255)

                                                                                                                          ccAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAAt              <  2:6218891/140‑1 (MQ=255)

                                                                                                                           cacgCCCCGCGACGCTCAGCATCAACACCACCAACATTTTGATTTCACTAGTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCCATAATAAACAAGGCGGCAATc             <  1:3261754/137‑1 (MQ=255)

                                                                                                                           cAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATc             >  1:5383486/1‑140 (MQ=255)

                                                                                                                           cAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATc             >  1:4033691/1‑140 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:4855978/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:1697689/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:1328932/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:1015414/140‑1 (MQ=255)

                                                                                                                               ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:5727857/140‑1 (MQ=255)

                                                                                                                                 ccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa       <  2:1636499/140‑1 (MQ=255)

                                                                                                                                 ccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa       <  1:5797221/140‑1 (MQ=255)

                                                                                                                                      aCGCTCAGCATCAACAAAACCACCATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTTCGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGtt  <  2:5457076/140‑1 (MQ=255)

TAATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414949‑2415222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`