BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,529,956	T→C	309 (TAA→TAG)	yfeR ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,529,956	0	T	C	100.0%	990.2 / NA	284	309 (TAA→TAG)	yfeR	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (171/113); total (171/113)

Only 100 of 284 total aligned reads displayed.

CACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCA‑‑‑‑‑‑‑‑‑CACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCA  >  NZ_CP025268/2529828‑2530091

cacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggctgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCta                                                                                                                                       >  2:4832909‑M2/134‑140 (MQ=255)

  cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaagtCACTCtata                                                                                                                                     >  2:1313117‑M2/132‑140 (MQ=255)

  cattactacggggatgcagtatcgatatgcggatgacaaactgggcgaagatttttcccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCTCTCtata                                                                                                                                     <  2:1963739‑M2/9‑1 (MQ=255)

  cagtactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                     <  2:2094852‑M2/9‑1 (MQ=255)

   attactacggggattcagtttcgatatgcggatgacaaactgggcgaagatttttaccatgatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccgtcagtatgaattCACTCTatat                                                                                                                                    <  2:1481747‑M2/10‑1 (MQ=255)

   attactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTatat                                                                                                                                    >  2:4986654‑M2/131‑140 (MQ=255)

   attactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTatat                                                                                                                                    >  2:3971694‑M2/131‑140 (MQ=255)

      actacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTg                                                                                                                                 <  1:2550005‑M2/13‑1 (MQ=255)

       ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                                >  1:750806‑M2/127‑140 (MQ=255)

       ctacggggattcagtatcgatatgccgatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaaatCACTCTATATCTGa                                                                                                                                >  1:6321598‑M2/127‑140 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                          >  1:383747‑M2/121‑140 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                          >  1:5773820‑M2/121‑140 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                          >  2:123113‑M2/121‑140 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                          >  2:2865585‑M2/121‑140 (MQ=255)

             ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                          >  2:934580‑M2/121‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  2:2941587‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:1188611‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:3109119‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:331085‑M2/119‑140 (MQ=255)

               attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                        >  1:4085008‑M2/119‑140 (MQ=255)

                       tcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCtt                                                                                                                >  1:2676583‑M2/111‑140 (MQ=255)

                         gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             >  1:1100066‑M2/109‑125 (MQ=255)

                         gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                              >  1:49186‑M2/109‑140 (MQ=255)

                          atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTccc                                                                                                             >  1:3023245‑M2/108‑140 (MQ=255)

                             tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                          <  2:3825707‑M2/36‑1 (MQ=255)

                             tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                          <  1:827986‑M2/36‑1 (MQ=255)

                             tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                          <  1:1808102‑M2/36‑1 (MQ=255)

                             tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                          >  1:3558183‑M2/105‑140 (MQ=255)

                             tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctacaccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                          <  2:6350912‑M2/36‑1 (MQ=255)

                              gcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                         <  1:4578590‑M2/37‑1 (MQ=255)

                               cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                             >  1:3437417‑M2/103‑119 (MQ=255)

                               cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                        >  1:174177‑M2/103‑140 (MQ=255)

                               cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                        <  1:945650‑M2/38‑1 (MQ=255)

                               cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                        >  1:5170717‑M2/103‑140 (MQ=255)

                                 gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                                      <  1:1296702‑M2/40‑1 (MQ=255)

                                  atgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGcc                                                                                                     >  1:3161974‑M2/100‑140 (MQ=255)

                                    gacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                                   >  2:1482709‑M2/98‑140 (MQ=255)

                                    gacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                                   >  1:2595281‑M2/98‑140 (MQ=255)

                                     acaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                                  >  2:3711207‑M2/97‑140 (MQ=255)

                                           tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                            <  1:5819644‑M2/50‑1 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                           >  1:5125353‑M2/90‑140 (MQ=255)

                                            gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                           >  1:4215879‑M2/90‑140 (MQ=255)

                                              gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTg                                                                                         >  1:5573603‑M2/88‑140 (MQ=255)

                                                gaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGcc                                                                                       <  1:2352662‑M2/55‑1 (MQ=255)

                                                 aagatttttaccaggatgcgctaatctcctccatcaaatttaacttctgattgagtactgccggggctcccggcagtatgaattCACTCTATCTCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  1:2872232‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  2:441010‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      <  1:6073631‑M2/56‑1 (MQ=255)

                                                 aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                      >  2:4681121‑M2/85‑140 (MQ=255)

                                                   gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                    <  1:2300937‑M2/58‑1 (MQ=255)

                                                   gattttgaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                    <  2:5853403‑M2/58‑1 (MQ=255)

                                                    atttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg                                                                                   <  2:1721013‑M2/59‑1 (MQ=255)

                                                           ccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTa                                                                            <  2:1050677‑M2/66‑1 (MQ=255)

                                                             aggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                          <  1:350688‑M2/68‑1 (MQ=255)

                                                              ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtctgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                                         >  2:3129562‑M2/72‑140 (MQ=255)

                                                              ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                                         >  1:5070707‑M2/72‑140 (MQ=255)

                                                               gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc                                                                        >  1:1645657‑M2/71‑140 (MQ=255)

                                                                atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                       <  1:238437‑M2/71‑1 (MQ=255)

                                                                atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                       <  2:2393804‑M2/71‑1 (MQ=255)

                                                                atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                       >  1:1488297‑M2/70‑140 (MQ=255)

                                                                             ctccatcatttttaacttctgcgtgattactgccggggctcccggcagtatgaagtCCCTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCCTTAGCGCAGTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                          >  2:6417597‑M2/57‑140 (MQ=255)

                                                                             ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                          >  2:4622983‑M2/57‑140 (MQ=255)

                                                                                catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                       >  2:4853190‑M2/54‑140 (MQ=255)

                                                                                catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                       <  2:325386‑M2/87‑1 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACaa                                                                                                                           >  2:2609153‑M2/52‑70 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     <  2:40089‑M2/89‑1 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     <  2:6059772‑M2/89‑1 (MQ=255)

                                                                                  tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                                     >  2:1342219‑M2/52‑140 (MQ=255)

                                                                                   caaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg                                                    <  2:2328358‑M2/90‑1 (MQ=255)

                                                                                       tttAACTTCTGATTGATGACTGCCGGGGCTCCCGGCAGGATGAAGTCACTCTAGATCTGATACAGCGGCTCCCCTGCCCGCCCAGCCCTGAGCGCATTGCCCGGGTCTCGTACCGCGTCCCATACCGCGTCTGcggcggg                                                >  2:6122215/1‑139 (MQ=255)

                                                                                       tctaacctctgactgcttactgccggggctcccggcagtatgaattCACTCTACCTCTGAGACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATACAGCTTCAGCGGCGGt                                                <  2:5671414‑M2/94‑1 (MQ=255)

                                                                                          aacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                             >  1:4718257‑M2/44‑140 (MQ=255)

                                                                                           acttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaa                                            <  2:4085008‑M2/98‑1 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATCTCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                       <  1:3079721‑M2/103‑1 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                       >  1:1656108‑M2/38‑140 (MQ=255)

                                                                                                tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                       >  1:5255330‑M2/38‑140 (MQ=255)

                                                                                                  attgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                                     >  2:4173892‑M2/36‑140 (MQ=255)

                                                                                                      attactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtt                                 >  1:2114370‑M2/32‑140 (MQ=255)

                                                                                                      attactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtt                                 >  1:4359198‑M2/32‑140 (MQ=255)

                                                                                                       ttactgccggggctcccggcagtatgaagtCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttc                                <  2:4511112‑M2/110‑1 (MQ=255)

                                                                                                          ctgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                             >  2:554532‑M2/28‑140 (MQ=255)

                                                                                                             ccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg                          <  1:6026561‑M2/116‑1 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  2:3408361‑M2/22‑140 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  2:955403‑M2/22‑140 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  1:4275103‑M2/22‑140 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  2:4743945‑M2/22‑140 (MQ=255)

                                                                                                                gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                       >  2:1988190‑M2/22‑140 (MQ=255)

                                                                                                                gggctcccggcagtatgaatnCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTGNAGCGGCGNTGGAAAGTGAACGGTTCTTCCTgcgc                       >  1:5637483‑M2/22‑140 (MQ=255)

                                                                                                                 ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                      <  2:5179247‑M2/120‑1 (MQ=255)

                                                                                                                  gctcccggcagtctgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAc                     >  1:1005263‑M2/20‑140 (MQ=255)

                                                                                                                    tcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa                   <  1:6252875‑M2/123‑1 (MQ=255)

                                                                                                                           agtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCaa            >  1:2423303‑M2/11‑140 (MQ=255)

                                                                                                                            gtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCGGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc           <  2:6170329‑M2/131‑1 (MQ=255)

                                                                                                                               tgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTgg        >  1:1448921‑M2/7‑140 (MQ=255)

                                                                                                                               tgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTgg        >  2:3443862‑M2/7‑140 (MQ=255)

                                                                                                                               tgaattCACTCTATATCTGATACAACGGATCACCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTgg        >  2:4283620‑M2/7‑140 (MQ=255)

                                                                                                                               tgaaatCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTATTCCTGCGCACCAGCATCAACTgg        >  2:1618846‑M2/7‑140 (MQ=255)

                                                                                                                                gaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGCCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGc       >  1:4989265‑M2/6‑140 (MQ=255)

                                                                                                                                  attCACTCTATATCTGATACAACGGTTCCCATTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCCTAAAGCTTAAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCCGCATCAACTGGCGt     >  2:740535‑M2/4‑140 (MQ=255)

                                                                                                                                     cacTCTATCTCTTATACAACTGATCCCCTTACCGCCCAGACATTAGCGCTGTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTca  <  2:1788857/140‑1 (MQ=255)

                                                                                                                                     cacTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTca  <  1:6381533/140‑1 (MQ=255)

CACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCA‑‑‑‑‑‑‑‑‑CACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCA  >  NZ_CP025268/2529828‑2530091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`