BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,622,099	T→C	234 (TAA→TAG)	hda ←	DnaA inactivator Hda

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,622,099	0	T	C	99.8%	1433.6 / NA	413	234 (TAA→TAG)	hda	DnaA inactivator Hda
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (220/191); minor base A (1/0); total (222/191)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 413 total aligned reads displayed.

GTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCCA  >  NZ_CP025268/2621965‑2622235

  gCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACt                                                                                                                                   >  2:3554325/1‑140 (MQ=255)

   cTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACtt                                                                                                                                  >  1:1333698/1‑140 (MQ=255)

        tCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACACTAAACACCTTATCTACAACTTCAGaa                                                                                                                             >  2:5958586/1‑140 (MQ=255)

        tCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGaa                                                                                                                             >  2:6222941/1‑140 (MQ=255)

           cTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAttt                                                                                                                          >  1:4910867/1‑140 (MQ=255)

             aGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAtttct                                                                                                                        >  1:2771936/1‑140 (MQ=255)

              gTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGACTACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAtttctt                                                                                                                       >  2:1919521/1‑140 (MQ=255)

               ttGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCTTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAtttcttt                                                                                                                      <  1:16064/140‑1 (MQ=255)

               ttGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAtttcttt                                                                                                                      <  1:3615757/140‑1 (MQ=255)

                tGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGAATCCGACAATAAACACCTTATCTACAACTTCAGAAtttctttc                                                                                                                     <  2:1716184/140‑1 (MQ=255)

                 gAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTca                                                                                                                    >  1:5023955/1‑140 (MQ=255)

                 gAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTca                                                                                                                    <  1:259331/140‑1 (MQ=255)

                  aaGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTcac                                                                                                                   >  2:142666/1‑140 (MQ=255)

                  aaGCACATTGCCGGATGCGACGATTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTcac                                                                                                                   <  2:4689468/140‑1 (MQ=255)

                   aGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACATTATCTACAACTTCAGAATTTCTTTcaca                                                                                                                  >  2:5611726/1‑140 (MQ=255)

                    gCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACaa                                                                                                                 <  1:3983481/140‑1 (MQ=255)

                      acaTTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAAc                                                                                                               <  1:3498939/140‑1 (MQ=255)

                          tGCCGGATGCGACGCTTGCCACGTCTTATACGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCATATCTACAACTTCAGAAATTATTTAACAAAAGGaa                                                                                                           >  2:4588501/1‑140 (MQ=39)

                           gCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAAt                                                                                                          <  1:4588501/140‑1 (MQ=255)

                           gCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAAt                                                                                                          <  1:3138786/140‑1 (MQ=255)

                           gCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAAt                                                                                                          <  2:5526115/140‑1 (MQ=255)

                             cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:767219/140‑1 (MQ=255)

                             cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  2:4860899/140‑1 (MQ=255)

                             cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:5236700/140‑1 (MQ=255)

                             cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:60301/140‑1 (MQ=255)

                              ggATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCNCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGt                                                                                                       <  1:5695477/140‑1 (MQ=255)

                                 tGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAg                                                                                                    >  1:2377963/1‑140 (MQ=255)

                                  gCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGCTAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACACCTTCAGAATTTCTTTCACAAACGGAATGGTCAGc                                                                                                   >  1:3081263/1‑140 (MQ=255)

                                  gCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGc                                                                                                   >  1:1917911/1‑140 (MQ=255)

                                    gACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTAGCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCtt                                                                                                 >  2:4027942/1‑140 (MQ=255)

                                       gCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACg                                                                                              <  2:3837982/140‑1 (MQ=255)

                                                gTCTTATCCGGCCTACTAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGt                                                                                     <  2:6065006/140‑1 (MQ=255)

                                                gTCTTATCCGGCCTACGAATACTAGGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACACTAAACACCTTATGTACAACTTCAGAAGTTCTTTCACAAACGGAAGGGGCAGCGTACGTTGCGCGGt                                                                                     >  2:1842296/1‑140 (MQ=255)

                                                     aTCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                                >  1:4659070/1‑140 (MQ=255)

                                                     aTCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                                >  1:5967898/1‑140 (MQ=255)

                                                     aTCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                                >  2:3364451/1‑140 (MQ=255)

                                                          gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:1508203/140‑1 (MQ=255)

                                                          gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:3742277/140‑1 (MQ=255)

                                                          gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:5849029/140‑1 (MQ=255)

                                                          gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  2:2956023/140‑1 (MQ=255)

                                                          gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:2177777/140‑1 (MQ=255)

                                                           ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  2:838095/1‑140 (MQ=255)

                                                           ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  2:5410512/1‑140 (MQ=255)

                                                           ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  1:3396089/1‑140 (MQ=255)

                                                               cGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATc                                                                      >  1:4330783/1‑140 (MQ=255)

                                                                  aTACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                                   <  1:2727452/140‑1 (MQ=255)

                                                                  aTACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                                   <  1:6266883/140‑1 (MQ=255)

                                                                    aCTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACt                                                                 >  1:1379762/1‑140 (MQ=255)

                                                                    aCTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACt                                                                 >  1:1757925/1‑140 (MQ=255)

                                                                     cTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTg                                                                >  1:5510634/1‑140 (MQ=255)

                                                                       aTGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGAt                                                              >  1:3453051/1‑140 (MQ=255)

                                                                        tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACTCCTTATCTACTAGTTCAGAATTTCTTTCTCATACGGAATGGTCAGCTTTCGTTGCGCGGTTATCGACGCACGATCCATCTGATc                                                             >  2:4483874/1‑140 (MQ=38)

                                                                        tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  2:5233828/97‑1 (MQ=255)

                                                                        tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             >  2:4997915/1‑140 (MQ=255)

                                                                        tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             <  1:934519/140‑1 (MQ=255)

                                                                        tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             >  1:2898816/1‑140 (MQ=255)

                                                                        tGGATTAACTACGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCGACAACTACAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             >  1:6334093/1‑140 (MQ=255)

                                                                         ggATTCACTTCTCGTAGTGCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTGCAGACTTTCGATCACCATCGGCCTGGGCAGCTTACGTTGCGCGGTCATCGTCGCACGATCCAACTGATcc                                                            >  2:961787/1‑140 (MQ=255)

                                                                         ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGTTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  2:5667690/1‑140 (MQ=255)

                                                                         ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  1:6177697/1‑140 (MQ=255)

                                                                          gATTAACTTCGCGTAGTTCTGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:163728/140‑1 (MQ=255)

                                                                          gATTAACTTCGCGTAGTTCGGATCTGGCGGTCGCGCCGCATCCGACAATAAACACCTTATCTTCAACTTCTGTTTTTCTTTCACAAACGGAATGGTAAGCTTACGTTGCGCGGTATTCGACGCACGTTCCAACTGATCCa                                                           <  2:2692435/140‑1 (MQ=255)

                                                                          gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  1:4308081/140‑1 (MQ=255)

                                                                          gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  1:5149500/140‑1 (MQ=255)

                                                                          gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:6306500/140‑1 (MQ=255)

                                                                          gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAAATGCAGAACTTCTTTCACAAACGGAATGGTCAGCTTACTTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:2481198/140‑1 (MQ=39)

                                                                            ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  1:4737883/140‑1 (MQ=255)

                                                                            ttAACTTAGCTTATTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  2:3502345/140‑1 (MQ=255)

                                                                               aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTc                                                      >  2:4010114/1‑140 (MQ=255)

                                                                               aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTc                                                      >  2:2101952/1‑140 (MQ=255)

                                                                                ctacgcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAGCGACGCACGATCCAACTGATCCAACGTCa                                                     >  2:1766317/4‑140 (MQ=255)

                                                                                cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCa                                                     >  1:1657802/1‑140 (MQ=255)

                                                                                cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCa                                                     >  2:4322684/1‑140 (MQ=255)

                                                                                   cgcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaa                                                  >  1:58884/1‑140 (MQ=255)

                                                                                    gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                                 >  2:5144926/1‑140 (MQ=255)

                                                                                    gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                                 >  1:987607/1‑140 (MQ=255)

                                                                                     cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  2:1341519/1‑140 (MQ=255)

                                                                                          ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                           >  1:2669554/1‑140 (MQ=255)

                                                                                               aTAAGGCGTTCGCGCGGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCa                                      >  1:56450/1‑140 (MQ=255)

                                                                                               aTAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCa                                      >  2:6116732/1‑140 (MQ=255)

                                                                                                  aGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCAttt                                   <  1:841309/140‑1 (MQ=255)

                                                                                                    gCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCCTTtct                                 >  1:2312316/1‑140 (MQ=255)

                                                                                                    gCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtct                                 >  1:409083/1‑140 (MQ=255)

                                                                                                        tCGGGCCGCATCCGACAATAAACTCCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTGACGGTGCGCGGTAATCGCCGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATATCTCTGt                             >  2:3713966/1‑140 (MQ=255)

                                                                                                              cgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGcc                       <  2:149216/140‑1 (MQ=255)

                                                                                                              cgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGcc                       <  2:5752758/140‑1 (MQ=255)

                                                                                                              cgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGcc                       <  1:657258/140‑1 (MQ=255)

                                                                                                              cgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGcc                       >  1:1037232/1‑140 (MQ=255)

                                                                                                                 aTCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCt                    <  1:3284408/140‑1 (MQ=255)

                                                                                                                 aTCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCt                    <  1:5353852/140‑1 (MQ=255)

                                                                                                                 aTCCGACAATAAACACCTTATCTACAACTTCAGAATTTCCTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATCAATAGCGTGCGCATTTCACTGTCGAGCCGCt                    <  2:671186/140‑1 (MQ=255)

                                                                                                                  tCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCtt                   <  2:2611582/140‑1 (MQ=255)

                                                                                                                           aaaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaa          >  1:4509968/1‑140 (MQ=255)

                                                                                                                            aaCACCTTATCTACAACTTCATAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  2:2887674/140‑1 (MQ=255)

                                                                                                                            aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  2:4177206/140‑1 (MQ=255)

                                                                                                                            aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  2:3115340/140‑1 (MQ=255)

                                                                                                                             acacATTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAAc        >  2:1273193/1‑140 (MQ=255)

                                                                                                                                ccTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGcc     >  2:917720/1‑140 (MQ=255)

                                                                                                                                   tATCTACAACTTCAGAATTTCTTTCACAACCGGAATGGTCAGCTTACGGTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  2:256442/140‑1 (MQ=255)

                                                                                                                                   tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGATTACGTTGCTCGTTAATCGACGCACGATCCAAATGATCCAACGTCATAAATAGCTTGCGAATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  2:761146/140‑1 (MQ=255)

GTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCCA  >  NZ_CP025268/2621965‑2622235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`