BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,828,512	T→C	N362S (AAC→AGC)	mltB ←	lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,828,512	0	T	C	99.5%	1261.1 / NA	364	N362S (AAC→AGC)	mltB	lytic murein transglycosylase B
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (125/236); minor base A (2/0); total (127/237)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 384 total aligned reads displayed.

TCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAC‑T‑TACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCC  >  NZ_CP025268/2828375‑2828646

  tGTCAGTCAAAAATTTATGCCGGACAAGAGAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGctac‑t                                                                                                                                       <  2:3479494/140‑1 (MQ=255)

   gtcagtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGctac‑tg                                                                                                                                      >  2:1952832/1‑139 (MQ=255)

   gtcagtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGGTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGctac‑tg                                                                                                                                      >  2:3878231/1‑139 (MQ=255)

       gtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAACAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGctac‑tgtact                                                                                                                                  <  2:2760666/140‑6 (MQ=255)

       gtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGctac‑tgtact                                                                                                                                  <  2:2328277/140‑6 (MQ=255)

            aaaTTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCgcgc                                                                                                                             >  2:6377050/1‑140 (MQ=255)

            aaaGTTATGCCGGACAAGATCGCACTGTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGTTTCAGAAACAAGCGGAGTCGCCAGAGGGAAGCTCACGCCCCCCTCGGGTAAATAGCTCC‑TGTACTCgcgc                                                                                                                             >  2:1084518/1‑140 (MQ=255)

             aaTTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCgcgcc                                                                                                                            <  2:6070852/140‑1 (MQ=255)

              aTTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCgcgcca                                                                                                                           <  2:3409217/140‑1 (MQ=255)

               tttATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCgcgccag                                                                                                                          <  1:6192979/140‑1 (MQ=255)

                   tGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCgcgccagcg‑cc                                                                                                                     <  2:6446690/140‑1 (MQ=255)

                   tGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCgcgccagcg‑cc                                                                                                                     <  1:6338338/140‑1 (MQ=255)

                        gACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGc                                                                                                                <  1:3980047/140‑1 (MQ=255)

                        gACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGc                                                                                                                >  1:674067/1‑140 (MQ=255)

                           aaGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTg                                                                                                            <  1:2408433/140‑1 (MQ=255)

                           aaGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTg                                                                                                            <  1:1737286/140‑1 (MQ=255)

                           aaGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTg                                                                                                            <  2:2463122/140‑1 (MQ=255)

                           aaGAGAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCCGAAAAAAGCTGATTAGCCAGAGGGACGCTCACGCCCCCCTCTTGTTAAGAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTg                                                                                                            <  2:264578/140‑1 (MQ=255)

                               tAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCt                                                                                                        <  1:860618/140‑1 (MQ=255)

                                aGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTa                                                                                                       >  2:4862056/1‑140 (MQ=255)

                                   aCTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGATGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACt                                                                                                    >  1:2079927/1‑140 (MQ=255)

                                     tttCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGc                                                                                                  >  1:4704256/1‑140 (MQ=255)

                                       tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGACGCTCACGCCCCCCTCGTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCa                                                                                                >  2:6252021/1‑140 (MQ=255)

                                       tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTCC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCa                                                                                                >  2:3158577/1‑140 (MQ=255)

                                       tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCa                                                                                                >  2:5984907/1‑140 (MQ=255)

                                       tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCa                                                                                                >  2:1421540/1‑140 (MQ=255)

                                       tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCa                                                                                                >  2:5641866/1‑140 (MQ=255)

                                       tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCa                                                                                                >  1:201398/1‑140 (MQ=255)

                                       tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGAGTAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑GTGTCCTAACTgacc                                                                                                >  1:5849012/1‑137 (MQ=255)

                                       tCGTCAGGAAGATGGGGTTGGCGTTGCCATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGACGCTCACGCCCCCCGCTTGTATATAGCGAC‑TGTACTCGCGCCCGCG‑CCACGGC‑TTGTCCTCACGGCCa                                                                                                >  2:1469477/1‑140 (MQ=255)

                                       tCGTCAGGAAGATGGGGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCa                                                                                                >  2:2014883/1‑140 (MQ=255)

                                          tCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGAc                                                                                             <  1:1002571/140‑1 (MQ=255)

                                             ggAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGc                                                                                          <  2:4252535/140‑1 (MQ=255)

                                              gAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGcc                                                                                         <  1:5061928/140‑1 (MQ=255)

                                              gAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGcc                                                                                         >  2:5475452/1‑140 (MQ=255)

                                              gAAGATGGTGTTGGCGTTGAAATTTTACGAGGAGGATTCAGTAAAAAGCTGATTAGCAAGAGGTAAGCTCACGCCCCCCACTTGTAAATAGATAC‑TGTAATAGCGCCAGCG‑CCACGTC‑TTGTCCTAACTGCAAGACCgac                                                                                         >  2:2083193/1‑138 (MQ=255)

                                               aaGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCa                                                                                        >  2:75881/1‑140 (MQ=255)

                                                aGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCAt                                                                                       <  1:2800256/140‑1 (MQ=255)

                                                    ggTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCg                                                                                   <  1:1230252/140‑1 (MQ=255)

                                                     gcggtGGCCTGGCAATTCTCCGCGGAGGAGACAGCAAAAAGCAGATTAGCCAGCGGGAAGCTCACGCCCCCCTCGTGTAACTAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGt                                                                                  <  2:5749094/136‑1 (MQ=255)

                                                        ttGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAAt                                                                               <  2:4044989/140‑1 (MQ=255)

                                                           gCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                                            <  1:4277293/140‑1 (MQ=255)

                                                              ttGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                                                         <  1:961113/140‑1 (MQ=255)

                                                               tGCAATTTTACGAGGAGGATTCAGAAAANAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                                        <  1:5678752/140‑1 (MQ=255)

                                                               tGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                                        <  1:5488283/140‑1 (MQ=255)

                                                               tGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                                        >  2:3620958/1‑140 (MQ=255)

                                                                gCAATTTTACGAGGAGGATTCAGAAAAATGCTGAGTAGCCAGAGGGAAGCTCACGCCGCCCTCTTGTTATTAGCTCCGG‑GTCGCGCGCCAGCG‑CCACGGC‑GTGTCCTAACTGCCAGACCGCCCTTGCGTAATGGGTgggg                                                                       >  2:4609070/1‑137 (MQ=255)

                                                                gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtg                                                                       <  2:5211546/140‑1 (MQ=255)

                                                                gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGAACACCCCCCCCACTTGTAAATCGCTAA‑T‑TAC‑CACCCCCCCGCCCCCGGCATTGACATAACTCCCAGACCGCCATGGCTAAATGGTTGAtg                                                                       >  2:118489/1‑140 (MQ=255)

                                                                              aggaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGt                                                         <  2:5892287/140‑1 (MQ=255)

                                                                               ggaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑CCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                                       <  1:3526909/140‑1 (MQ=255)

                                                                                gaTTCAGAAAAAAGCTGATTAGCCAGAGGGACGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCAGTGCGAAATGGGTGCTGTGGTTGTAACGGCTGa                                                       <  2:1757031/140‑1 (MQ=255)

                                                                                gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                                       <  1:1157344/140‑1 (MQ=255)

                                                                                gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                                       <  1:593540/140‑1 (MQ=255)

                                                                                gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                                       <  1:6061179/140‑1 (MQ=255)

                                                                                gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                                       <  1:3284570/140‑1 (MQ=255)

                                                                                gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                                       <  1:3093216/140‑1 (MQ=255)

                                                                                  ttCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATg                                                     >  1:4792496/1‑140 (MQ=255)

                                                                                      gAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGgtgt                                                 <  1:2211348/140‑1 (MQ=255)

                                                                                                gATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCT‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCgg                                       <  2:1273190/140‑1 (MQ=255)

                                                                                                gATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCgg                                       <  1:2670880/140‑1 (MQ=255)

                                                                                                gATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCgg                                       <  1:826600/140‑1 (MQ=255)

                                                                                                gATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCgg                                       <  1:1394313/140‑1 (MQ=255)

                                                                                                gATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCgg                                       <  1:3638582/140‑1 (MQ=255)

                                                                                                     gCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTCCTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                  <  2:214009/140‑1 (MQ=255)

                                                                                                     gCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                  <  2:2893754/140‑1 (MQ=255)

                                                                                                     gCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                  <  1:1810170/140‑1 (MQ=255)

                                                                                                     gCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                  <  1:1435308/140‑1 (MQ=255)

                                                                                                     gCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                  <  1:4113570/140‑1 (MQ=255)

                                                                                                     gCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                  <  2:1591123/140‑1 (MQ=255)

                                                                                                     gCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAAGAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                  <  2:4275583/140‑1 (MQ=255)

                                                                                                         gagGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa                              <  1:2175352/140‑1 (MQ=255)

                                                                                                          agGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc                             >  1:4475778/1‑140 (MQ=255)

                                                                                                          agGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc                             <  2:1887866/140‑1 (MQ=255)

                                                                                                          agGGAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc                             <  1:3000187/140‑1 (MQ=255)

                                                                                                           gggAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc                            <  1:1639333/140‑1 (MQ=255)

                                                                                                           gggAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc                            <  2:5095868/140‑1 (MQ=255)

                                                                                                           gggAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc                            <  2:4941189/140‑1 (MQ=255)

                                                                                                           gggAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc                            <  2:371775/140‑1 (MQ=255)

                                                                                                           gggAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTGGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAcc                            >  2:2675692/1‑140 (MQ=255)

                                                                                                             gAAGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAg                          <  1:5568977/140‑1 (MQ=255)

                                                                                                              aaGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGt                         >  1:725142/1‑140 (MQ=255)

                                                                                                               aGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTa                        <  2:1711743/140‑1 (MQ=255)

                                                                                                               aGCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTa                        >  1:5989987/1‑140 (MQ=255)

                                                                                                                gCTCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTAc                       >  1:2762319/1‑140 (MQ=255)

                                                                                                                  tCACGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTg                     <  1:4411619/140‑1 (MQ=255)

                                                                                                                    aCGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGt                   >  1:5238853/1‑140 (MQ=255)

                                                                                                                    aCGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGt                   >  1:385288/1‑140 (MQ=255)

                                                                                                                    aCGCCCCCCTCTTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGt                   >  1:5601687/1‑140 (MQ=255)

                                                                                                                        cccccTCTTGTAAATAGCGAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGcc               <  2:2227896/140‑1 (MQ=255)

                                                                                                                             tctTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGc          <  2:5385077/140‑1 (MQ=255)

                                                                                                                              ctTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGcc         <  1:2610826/140‑1 (MQ=255)

                                                                                                                              ctTGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGcc         <  1:2480489/140‑1 (MQ=255)

                                                                                                                              ctTGAAAATAGCTAC‑TGCACTCGCGCCAGCG‑CCACGGC‑TTGACCTACCTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGGAGCCGGTGcc         <  2:1527235/140‑1 (MQ=255)

                                                                                                                                tGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCaa       <  1:456020/140‑1 (MQ=255)

                                                                                                                                tGTAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATAGCGTAATCGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCaa       >  1:2290129/1‑140 (MQ=255)

                                                                                                                                  tAAATAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTGGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGTTAGCCGGTGCCAACa     >  2:3456271/1‑140 (MQ=255)

                                                                                                                                    aaTAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTACCGGGTGATGGTGTAGAAGTACGGCAGACCGTACCAGTACTGGTAGCCGGTGccaccagc   >  2:4346980/1‑138 (MQ=255)

                                                                                                                                    aaTAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATc   >  2:3289550/1‑140 (MQ=255)

                                                                                                                                     aTAGCTAC‑TGTACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTACTGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATcc  <  1:4932738/140‑1 (MQ=255)

TCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAC‑T‑TACTCGCGCCAGCG‑CCACGGC‑TTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCC  >  NZ_CP025268/2828375‑2828646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`