BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,869,477	A→C	I128S (ATC→AGC)	rpoS ←	RNA polymerase sigma factor RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,869,477	0	A	C	100.0%	1262.9 / NA	361	I128S (ATC→AGC)	rpoS	RNA polymerase sigma factor RpoS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (196/165); total (196/165)

Only 100 of 361 total aligned reads displayed.

CTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGC  >  NZ_CP025268/2869341‑2869613

cTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCa                                                                                                                                       >  1:16033/1‑140 (MQ=255)

  ttACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGc                                                                                                                                     >  1:5800977/1‑140 (MQ=255)

   tACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGcc                                                                                                                                    <  1:4439874/140‑1 (MQ=255)

   tACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGcc                                                                                                                                    <  1:4554580/140‑1 (MQ=255)

   tACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGcc                                                                                                                                    <  2:763551/140‑1 (MQ=255)

       aTGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAg                                                                                                                                >  1:2486281/1‑140 (MQ=255)

       aTGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAg                                                                                                                                >  2:3848462/1‑140 (MQ=255)

        tgtgAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAgg                                                                                                                               <  1:3407590/140‑1 (MQ=255)

        tgtgAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCGCTACCGCGCGGCTCAGCCCCAgg                                                                                                                               <  2:1914418/140‑1 (MQ=255)

          tgAATCGGCAAACGAATAGTACGGGTTTTGTTCATAATCGACCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTTAGAACCGGAAACCACGTTCCGGGTCAAACTTATCTACCGCGCGGCTCAGCCCCAGGtt                                                                                                                             >  2:4170729/1‑140 (MQ=255)

          tgAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCNAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGtt                                                                                                                             >  1:5687125/1‑140 (MQ=255)

          tgAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGtt                                                                                                                             >  1:5627388/1‑140 (MQ=255)

          tgAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGtt                                                                                                                             >  1:180473/1‑140 (MQ=255)

           gAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTg                                                                                                                            <  2:6341930/140‑1 (MQ=255)

           gAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGGTCCGGGTCAAACTTCTCTCCCGCGCGGCTCAGCCCCAGGTTg                                                                                                                            <  2:614450/140‑1 (MQ=255)

            aaTCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGc                                                                                                                           >  1:322411/1‑140 (MQ=255)

             aTCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCTATCGTCTGGCTAATCCACCAGGTTGCGTATGTTGAGAAGAGGAAACCACGTTCAGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGcc                                                                                                                          <  2:2551126/140‑1 (MQ=255)

             aTCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGcc                                                                                                                          <  2:1160983/140‑1 (MQ=255)

             aTCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGcc                                                                                                                          <  2:2676230/140‑1 (MQ=255)

             aTCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGcc                                                                                                                          <  2:5307611/140‑1 (MQ=255)

             aTCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGcc                                                                                                                          <  2:446544/140‑1 (MQ=255)

               cGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCct                                                                                                                        >  2:4023872/1‑140 (MQ=255)

                ggCAAACGAATAGTACGGGTTTGGTTCATACTCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCctc                                                                                                                       <  2:4809644/140‑1 (MQ=255)

                ggCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCctc                                                                                                                       <  1:6257170/140‑1 (MQ=255)

                    aaCGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCg                                                                                                                   >  2:5775012/1‑140 (MQ=255)

                      cGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGAt                                                                                                                 <  2:354030/140‑1 (MQ=255)

                      cGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGAt                                                                                                                 <  1:3018778/140‑1 (MQ=255)

                       gAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATa                                                                                                                <  2:1255175/140‑1 (MQ=255)

                       gAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATa                                                                                                                <  2:696169/140‑1 (MQ=255)

                          tAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAgg                                                                                                             <  1:1318059/140‑1 (MQ=255)

                           aGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGt                                                                                                            >  2:2133473/1‑140 (MQ=255)

                           aGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGt                                                                                                            >  2:4289414/1‑140 (MQ=255)

                                gggTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGc                                                                                                       <  2:5087158/140‑1 (MQ=255)

                                 ggTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCa                                                                                                      <  1:4601291/140‑1 (MQ=255)

                                  gTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCaa                                                                                                     >  1:6423798/1‑140 (MQ=255)

                                  gTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCaa                                                                                                     >  1:4864581/1‑140 (MQ=255)

                                  gTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCaa                                                                                                     <  1:2823207/140‑1 (MQ=255)

                                  gTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCaa                                                                                                     >  1:900655/1‑140 (MQ=255)

                                     tGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGc                                                                                                  >  1:1152698/1‑140 (MQ=255)

                                       gTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCa                                                                                                >  1:5247660/1‑140 (MQ=255)

                                       gTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCGCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCa                                                                                                >  1:3821703/1‑140 (MQ=255)

                                            tAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCa                                                                                           >  1:1502468/1‑140 (MQ=255)

                                              aTCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACg                                                                                         <  1:3120851/140‑1 (MQ=255)

                                                cGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAt                                                                                       <  1:3848462/140‑1 (MQ=255)

                                                cGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAt                                                                                       <  1:5036426/140‑1 (MQ=255)

                                                 gCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAtt                                                                                      >  2:2949689/1‑140 (MQ=255)

                                                  cccGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTg                                                                                     >  1:2404980/1‑140 (MQ=255)

                                                  cccGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTg                                                                                     >  1:1005733/1‑140 (MQ=255)

                                                  cccGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACGTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTg                                                                                     >  2:5597430/1‑140 (MQ=255)

                                                    cGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGcc                                                                                   >  1:3057066/1‑140 (MQ=255)

                                                      ttCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCCCGTCCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCCGCAACGCCAGACCACGATTGCCAt                                                                                 >  2:4869747/1‑140 (MQ=255)

                                                      ttCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCAt                                                                                 >  2:4697087/1‑140 (MQ=255)

                                                      ttCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCAt                                                                                 >  1:1996761/1‑140 (MQ=255)

                                                          aTCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcg                                                                             <  1:4375684/140‑1 (MQ=255)

                                                               cTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCggg                                                                        <  1:5978901/140‑1 (MQ=255)

                                                               cTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCggg                                                                        <  1:173496/140‑1 (MQ=255)

                                                                tGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGc                                                                       >  1:1189081/1‑140 (MQ=255)

                                                                     aaTCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAttt                                                                  >  2:3433175/1‑140 (MQ=255)

                                                                       tCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAttttt                                                                >  2:5139879/1‑140 (MQ=255)

                                                                       tCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAttttt                                                                >  2:856161/1‑140 (MQ=255)

                                                                       tCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAttttt                                                                >  1:1536494/1‑140 (MQ=255)

                                                                       tCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAttttt                                                                >  1:3984150/1‑140 (MQ=255)

                                                                               gTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAg                                                        <  1:3641025/140‑1 (MQ=255)

                                                                                 tGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGAc                                                      >  1:2153811/1‑140 (MQ=255)

                                                                                 tGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGAc                                                      >  2:452943/1‑140 (MQ=255)

                                                                                   cGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGc                                                    >  1:4122423/1‑140 (MQ=255)

                                                                                      aTGTTGAGTAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTTCCATAACGGCGGGCAATTTTTACCACCAGACGCAAg                                                 <  2:4192987/140‑1 (MQ=255)

                                                                                      aTGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAg                                                 <  1:5479473/140‑1 (MQ=255)

                                                                                      aTGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACGTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAg                                                 <  2:501400/140‑1 (MQ=255)

                                                                                          tGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTAc                                             >  1:3471569/1‑140 (MQ=255)

                                                                                           gagaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCTGACCACGATTGCCATAACGGCGGGCAATGTTTACCACCAGACGCAGGTGAct                                            >  2:3035668/1‑140 (MQ=255)

                                                                                             gaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTActct                                          >  2:2273401/1‑140 (MQ=255)

                                                                                             gaAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTActct                                          >  1:2289967/1‑140 (MQ=255)

                                                                                                        cACGTTCCGGGGCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATCAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATGTTTACCACCAGACGCAAGTTACTCTCGATCATCcgg                               >  2:1344107/1‑140 (MQ=255)

                                                                                                           gTTCCGGGTCAATCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCcggcga                            <  2:2807650/140‑2 (MQ=255)

                                                                                                              ccGGGTCAAACTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCga                         <  2:5687125/140‑1 (MQ=255)

                                                                                                                     aaaCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGa                  <  2:5923905/140‑1 (MQ=255)

                                                                                                                     aaaCGTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGa                  <  1:5370002/140‑1 (MQ=255)

                                                                                                                      aaCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGAc                 >  1:4898733/1‑140 (MQ=255)

                                                                                                                       aCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATCAGGTCCAGCAACGCCAGCCCACGATTCCCATAACCGCGCGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACa                <  2:1685174/140‑1 (MQ=255)

                                                                                                                       aCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACa                <  1:1451312/140‑1 (MQ=255)

                                                                                                                       aCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACa                <  1:2547847/140‑1 (MQ=255)

                                                                                                                       aCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACa                <  1:657021/140‑1 (MQ=255)

                                                                                                                       aCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACa                <  2:3904760/140‑1 (MQ=255)

                                                                                                                       aCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACa                <  1:829887/140‑1 (MQ=255)

                                                                                                                       aCTTCTCTACCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAATTACTCTCGATCATCCGGCGGCGAGAGGCGACa                <  2:5470506/140‑1 (MQ=255)

                                                                                                                       aCTTCTCTACCGCGCGGCTCAGCCCCAGGGTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACa                <  2:4312879/140‑1 (MQ=255)

                                                                                                                               aCCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATTAGGTCCAGCATCGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCTTGTTACTCTCGATCATCCGGCGGCGAGAGGCGTCATCTCCTCg        <  2:1632545/140‑1 (MQ=255)

                                                                                                                               aCCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACg        <  1:426126/140‑1 (MQ=255)

                                                                                                                               aCCGCGCGGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACg        <  2:6384082/140‑1 (MQ=255)

                                                                                                                                 cgcgcgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCa      >  2:1825462/1‑140 (MQ=255)

                                                                                                                                 cgcgcgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCa      >  2:3485506/1‑140 (MQ=255)

                                                                                                                                   cgcgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGt    >  1:3362920/1‑140 (MQ=255)

                                                                                                                                   cgcgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGt    >  1:2721579/1‑140 (MQ=255)

                                                                                                                                   cgcgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGt    >  1:2699728/1‑140 (MQ=255)

                                                                                                                                   cgcgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGt    >  1:4791598/1‑140 (MQ=255)

                                                                                                                                   cgcgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGt    >  2:2365550/1‑140 (MQ=255)

                                                                                                                                   cgcgGCTCAGCCCCAGGTTGCCCGCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCACGTGACTCTCGATCATCCGGCGGCGAGAGGCGACAGCTCCACGCAgg    >  2:6052994/1‑139 (MQ=255)

                                                                                                                                     cgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCCGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACAGCTCCACGCAGTgc  >  2:4578262/1‑140 (MQ=255)

                                                                                                                                     cgGCTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTgc  >  1:1385151/1‑140 (MQ=255)

CTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGC  >  NZ_CP025268/2869341‑2869613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`