BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,986,326	C→T	C35Y (TGC→TAC)	kduI ←	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,986,326	0	C	T	100.0%	1368.7 / NA	351	C35Y (TGC→TAC)	kduI	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (81/270); total (81/270)

Only 100 of 351 total aligned reads displayed.

TAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGAAAA  >  NZ_CP025268/2986189‑2986461

    acCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTGGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGGATTATGCCGCCTACATGAATTCGGTCAATGTGGCTGTAAACCATGGTGTAGTCAt                                                                                                                                    >  2:1604815/1‑140 (MQ=255)

      cTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCg                                                                                                                                  >  1:5233755/1‑140 (MQ=255)

       tAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCgg                                                                                                                                 >  2:288501/1‑140 (MQ=255)

        aaCTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGc                                                                                                                                <  2:3102927/140‑1 (MQ=255)

        aaCTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGc                                                                                                                                >  1:4433859/1‑140 (MQ=255)

         aCTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCg                                                                                                                               >  2:440715/1‑140 (MQ=255)

             gcgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTGAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACaa                                                                                                                           >  1:5793007/1‑140 (MQ=255)

              cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACaaa                                                                                                                          >  2:870093/1‑140 (MQ=255)

              cgACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACaaa                                                                                                                          >  1:2505129/1‑140 (MQ=255)

               gACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACaaag                                                                                                                         <  2:1718241/140‑2 (MQ=255)

               gACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAAt                                                                                                                         <  2:314212/140‑1 (MQ=255)

                      aaGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCtttt                                                                                                                  <  1:1838798/140‑1 (MQ=255)

                             tAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCaa                                                                                                           >  2:3623914/1‑140 (MQ=255)

                                    cGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCa                                                                                                    >  2:657742/1‑140 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACTGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAAGAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATCCCTTTTCAACCAAAAATTCATTgcg                                                                                               <  2:729382/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  2:1292813/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:1150162/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               >  2:3732760/1‑140 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:4780129/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:4608760/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:1435320/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:4025777/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:3815294/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:377182/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  2:4725806/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:2172266/140‑1 (MQ=255)

                                         aGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  1:2806671/140‑1 (MQ=255)

                                         aGTTGTTTAACAACTTCCCCGAAAAATGAAAAAGATTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTTGATTTAAACCATGGTTTAATCATCTGATACAAATACCTTTTCAACCAAAAATTCATTgcg                                                                                               <  2:3261955/140‑1 (MQ=255)

                                          gttgttTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTgcgc                                                                                              >  2:5530526/1‑140 (MQ=255)

                                            tgttTACCAACTTCCCCGACAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAg                                                                                            <  2:2444213/140‑1 (MQ=255)

                                              tttACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGcc                                                                                          >  2:6336422/1‑140 (MQ=255)

                                                   cAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTgg                                                                                     >  2:1402877/1‑140 (MQ=255)

                                                     aCTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGt                                                                                   >  2:2639362/1‑140 (MQ=255)

                                                        tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATc                                                                                >  1:4553895/1‑140 (MQ=255)

                                                        tCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCATTTCAACCAAAAATTCATTTCGCAGCCCTTGGGTATc                                                                                <  2:6121576/140‑1 (MQ=255)

                                                          cccGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCa                                                                              <  1:3628035/140‑1 (MQ=255)

                                                          cccGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCa                                                                              <  2:5705725/140‑1 (MQ=255)

                                                          cccGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCATTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCa                                                                              <  2:3363828/140‑1 (MQ=255)

                                                                 aCGGAAACCGTTTTAGTTATCGGCATCATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCATTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGtt‑tt                                                                      <  1:5555512/140‑1 (MQ=255)

                                                                   ggAAACCGTTTTAGTTATCGGCATAATTCCGCCAACAATAATTCGGTCAATGTGGCTGTATACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTtg                                                                    <  2:619551/140‑1 (MQ=255)

                                                                      aaCCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTtgcgt                                                                 <  1:2290869/140‑1 (MQ=255)

                                                                       cccGATTTAGTTATCGGCATACTGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TGtgcgtg                                                                <  2:1563682/139‑1 (MQ=255)

                                                                       aCCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTtgcgtg                                                                <  2:1344287/140‑1 (MQ=255)

                                                                       aCCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTtgcgtg                                                                <  2:4526523/140‑1 (MQ=255)

                                                                       aCCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTtgcgtg                                                                <  1:1564320/140‑1 (MQ=255)

                                                                       aCCGTTTTAGTTATCGGCAGAATGCCGCCAACAATAATTCGGTCACTGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTtgcgtg                                                                <  2:4003367/140‑1 (MQ=255)

                                                                       aCCGTTTTAGTTAACGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTtgcgtg                                                                <  2:4117849/140‑1 (MQ=255)

                                                                         cGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTtgcgtgcg                                                              <  2:4250074/140‑1 (MQ=255)

                                                                          gTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTgcgc                                                             <  1:6326375/140‑1 (MQ=255)

                                                                           ttttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCa                                                            <  1:3615009/140‑1 (MQ=255)

                                                                           ttttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCa                                                            <  2:3211761/140‑1 (MQ=255)

                                                                           ttttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCa                                                            <  2:5467485/140‑1 (MQ=255)

                                                                            tttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCAc                                                           <  1:4687087/140‑1 (MQ=255)

                                                                            tttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCAc                                                           <  1:5844294/140‑1 (MQ=255)

                                                                            tttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCAc                                                           <  2:5092755/140‑1 (MQ=255)

                                                                            tttAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCAc                                                           <  2:4777043/140‑1 (MQ=255)

                                                                            tttAGTTAACGGCATACTGCCGCCAACAATAATTCTGTCAATGTTGCTGTAAACCATGGTGTACTCAACGGCGACAACTACCTTTTCAACCAAAAAGTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCAc                                                           <  2:2535828/140‑1 (MQ=255)

                                                                               aGTTATCGGCATAATGCCGCCAACAATAATTCGGTCACTGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACtgt                                                        <  2:5185680/140‑1 (MQ=255)

                                                                                 ttATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTgg                                                      <  2:4642651/140‑1 (MQ=255)

                                                                                  tATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGa                                                     <  1:1983321/140‑1 (MQ=255)

                                                                                  tATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGCGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGa                                                     <  1:6005457/140‑1 (MQ=255)

                                                                                   aTCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGAt                                                    <  1:1322742/140‑1 (MQ=255)

                                                                                   aTCGGCATAATGCCGCCAACAATAATTCGGTAAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTGTTTGCGTGCCCAGTGTggt                                                     >  2:2006307/1‑139 (MQ=255)

                                                                                     cGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGc                                                  <  2:5428409/140‑1 (MQ=255)

                                                                                      gtcATAATGCCGCCAACAATAAGTCGCACAATGTGTCTGTAAACCATGGTGTACTCCTCGGCGACAAATACCTTTTCAACCAAACATTCATTGCGCAGCCCTTGGGTATCCAGCGAT‑TTTGCGTGCGCACTGTGGATGct                                                 <  2:932294/138‑1 (MQ=255)

                                                                                      ggCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGct                                                 <  1:3254742/140‑1 (MQ=255)

                                                                                      ggCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGct                                                 <  1:3720085/140‑1 (MQ=255)

                                                                                      ggCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGct                                                 <  1:834140/140‑1 (MQ=255)

                                                                                      ggCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGct                                                 <  2:3497957/140‑1 (MQ=255)

                                                                                      ggCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGct                                                 <  1:6065487/140‑1 (MQ=255)

                                                                                       gCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGctc                                                <  1:3902862/140‑1 (MQ=255)

                                                                                       gCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTATCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGctc                                                <  1:2359191/140‑1 (MQ=255)

                                                                                            aTGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCt                                           <  1:1195284/140‑1 (MQ=255)

                                                                                            aTGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCt                                           <  2:5737631/140‑1 (MQ=255)

                                                                                              gccgccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTa                                         >  2:3907551/1‑140 (MQ=255)

                                                                                                cgccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACg                                       >  2:1013697/1‑140 (MQ=255)

                                                                                                cgccAACAATAATTCGCTCAATGTCTCTTTAAACCATGGTCTACACAACGGCGACAAATACCCATTCAACCAAAAATTCATTGCGCAGCCCTTGGGAATCCAGCGTT‑TTTGCGTGCGCCCTGTGGATGCTCTGTCTTACg                                       <  2:2368377/140‑1 (MQ=255)

                                                                                                 gccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGt                                      <  1:3156452/140‑1 (MQ=255)

                                                                                                  ccAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTc                                     <  1:416465/140‑1 (MQ=255)

                                                                                                   caacaaTAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTcc                                    <  1:3684947/140‑1 (MQ=255)

                                                                                                           atTCGGTCACTGTGGCTGTAAACCAGGGTGTACTCCTCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACGGTGGATGCACTGTCTTACGTCCACATCAAc                            <  2:4176781/140‑1 (MQ=255)

                                                                                                           atTCGGTCACAGAGGCGGTACACCATGGTGTACTCAACGGCGACAACGACCATTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGAT‑TTTGCGTGCGCACTGTGGATGCTCGGTCGTCCGTCCACATCacc                            <  2:5602523/140‑3 (MQ=255)

                                                                                                           atTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAAc                            <  2:1779409/140‑1 (MQ=255)

                                                                                                           atTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAAc                            <  1:342177/140‑1 (MQ=255)

                                                                                                           atTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAAc                            <  2:5016895/140‑1 (MQ=255)

                                                                                                              cGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTc                         >  2:1853090/1‑140 (MQ=255)

                                                                                                                gTCACTGTGGCTGTAAACCATGGGGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTNTCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                       <  1:5687993/140‑1 (MQ=255)

                                                                                                                gTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                       <  1:311943/140‑1 (MQ=255)

                                                                                                                gTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                       <  1:2617597/140‑1 (MQ=255)

                                                                                                                gTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                       <  1:6156862/140‑1 (MQ=255)

                                                                                                                gTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCg                       <  1:729964/140‑1 (MQ=255)

                                                                                                                 tCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGa                      >  2:5303793/1‑140 (MQ=255)

                                                                                                                  cAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGAt                     <  1:192989/140‑1 (MQ=255)

                                                                                                                         gCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGt              >  1:4977970/1‑140 (MQ=255)

                                                                                                                         gCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGt              >  1:101684/1‑140 (MQ=255)

                                                                                                                           tGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGa            <  1:2965495/140‑1 (MQ=255)

                                                                                                                           tGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGa            <  1:2726554/140‑1 (MQ=255)

                                                                                                                               aaCCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCg        >  1:3018728/1‑140 (MQ=255)

                                                                                                                               aaCCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCg        >  1:2127990/1‑140 (MQ=255)

                                                                                                                                     ggTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGaaaa  <  1:1272604/140‑1 (MQ=255)

TAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGCACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTT‑TTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACCTCCGATAATCAGTGAAGCGTGAAAA  >  NZ_CP025268/2986189‑2986461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`