BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,174,183	T→C	111 (TAA→TAG)	ygiZ ←	DUF2645 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,174,183	0	T	C	99.6%	781.0 / NA	228	111 (TAA→TAG)	ygiZ	DUF2645 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (75/151); minor base A (0/1); total (75/153)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

CGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGC  >  NZ_CP025268/3174046‑3174318

cgtttGTCCAGCGAATCGCCAAATTGCATGGCATGATTGTTGAATTGGGGAATGCGGAACAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTTTGTGAGCCGTTAACACGACTCTCATAGATTttacta                                                                                                                                       <  1:473415/137‑1 (MQ=255)

 gATTGTCCAGCGAATCGCCAAATGGCATGGCATGAATGTTGAATTTGGGAATGCGTAACACGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                                                                                      <  2:1101577/140‑1 (MQ=255)

 gATGGTCCAGCGAATCGCCAAATTGCATGGCTTGAATGTTGAATTTGGGAAGGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                                                                                      <  2:3354689/140‑1 (MQ=255)

     gTCCAGCTAATCGCCAATTTGCATGGCATGAATGTTTAATTTGGGAATGCGGAACATGGTGGATTTGAGGCTAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  <  1:5267234/140‑1 (MQ=255)

     gTCCAGCTAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGTATTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  <  1:4387493/140‑1 (MQ=255)

     gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTTAATTTGGGAATGCGGAACAAGGTGTATTTGAGGCTAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  <  1:1546534/140‑1 (MQ=255)

      tcccccGAATCGCCAAATTGCCTGGCATGACTGTTGAATTTGGGTATGCGCACCAAGGTGCATTTGAGGCGATGGTAAGATGGAAACGTCACTGTCTATGAGAGCCGTTAAAACGACGCTCATAGATTTTACGAATAGCa                                                                                                                                 <  2:5797140/135‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGCTTGGAATTAATGTTTAATTTGGTAATGCGGAACAAGGTGTATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATTAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  1:647551/140‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGTATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:1666838/140‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:408625/140‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:3593713/140‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  1:2555551/140‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:5475879/140‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGCATGGAATGATTTTTTAATTTGGTATTGCGGAACAAGGTGGATTTGCGGCTAAGTTAAGCTGGTAACTTCTCTGTTTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAGTAATAGCa                                                                                                                                 <  1:5718920/140‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGCAGGGCATGAATGTTGAATGTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTATATGACAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:4830199/140‑1 (MQ=255)

      tCCAGCGAATCGCCAAATTGAATGGAATGAATGTTTATTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:5674823/140‑1 (MQ=255)

       ccAGCTAATCGACAATTTGCATGGAATGATTTTTTAATTTGGGAATGCGTAACATGGTGGTTTTGTGGCGAAGGTAAGCTGTTAACTTCTCTTTATATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                <  1:1183948/140‑1 (MQ=255)

         aGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaaa                                                                                                                              >  1:4927452/1‑140 (MQ=255)

                cGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                                                                                       >  1:5102058/1‑140 (MQ=255)

                cGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                                                                                       >  2:3280039/1‑140 (MQ=255)

                  ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCg                                                                                                                     >  1:557594/1‑140 (MQ=255)

                              gcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                                                                         <  1:6186490/140‑1 (MQ=255)

                                  gactGTTGAATTTGGGACTGCGGAACCAGGTGGATTTGAGGCGAATGTAAGCTGGTAACTTCTCTGTCGATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                                                                                     <  2:5051349/137‑1 (MQ=255)

                                  gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                                                                                     <  2:1185104/140‑1 (MQ=255)

                                   aaTGTTTAATTTGGGAATGCGTAACAAGGTGTATTTGCGGCGATGTTAAGATGTTAACTTCACTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                                                    <  1:3781518/140‑1 (MQ=255)

                                   aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCACTGTCTATTAGAGCCGTTAAAACGACTCTCATAGATATTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                                                    <  2:6389174/140‑1 (MQ=255)

                                       ctGACTTCGGGAATGCGGAACACGGTGGATTTGCGGCCAAGCAAAGCTGGTCACTTCTCTGACTATCAGAGCCGATCAAACGACTCACATAGATTTTCCTAATCGCCCAATATAAACCGTCCCCAAAAAAGCCaccaacc                                                                                                <  2:4871654/139‑1 (MQ=255)

                                         gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  2:4011290/1‑140 (MQ=255)

                                         gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  2:6427908/1‑140 (MQ=255)

                                         gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  1:2119007/1‑140 (MQ=255)

                                         gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  2:4996916/1‑140 (MQ=255)

                                                 gAATGGGGAACAAGGTGGTTTTGTGGCGAAGGTATGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                                                                                      <  2:2987698/140‑1 (MQ=255)

                                                     gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaaa                                                                                  >  1:4212985/1‑140 (MQ=255)

                                                        gAACATGGTGGTTTTGAGGCGATGGTACGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                                                                               <  1:351942/140‑1 (MQ=255)

                                                           cAAGGTGTTTTTGTGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                                                                            <  1:3771098/140‑1 (MQ=255)

                                                            atggTGTATTTGAGGCGAAGTTAAGCTGGTAACTTCTCTGTCTATTAGTGACTTTAAAACGACTATCATAGATTTTAATAATAGCAAAATATAAACCGTCCACAAAAAATACACAAACCAAAAACCTGACACATAGcgtc                                                                           <  2:2436197/138‑3 (MQ=255)

                                                            acggaggATATGAGGCGAAGGTACGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  2:6147752/135‑1 (MQ=255)

                                                            acggTGGATTTGCGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACACACCAGACAAATACcgag                                                                           <  1:2428381/138‑1 (MQ=255)

                                                            aaGTTGTATTTTAGTCTAAGTTAAGATGTTAAATTCTCTGTCTATTAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACagat                                                                           <  2:4890325/140‑2 (MQ=255)

                                                            aaGGTGGTTTTGAGGCTAAGGTATGCTGTTAATTTCTCTGTCTTTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:2819814/140‑1 (MQ=255)

                                                            aaGGTGGATTTGAGGCGAAGGTATGCTGGTAACTTCTCGGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACACACCAGACAAAGACcgag                                                                           <  2:1937290/140‑1 (MQ=255)

                                                            aaGGTGGATTTGAGGCGAAGGTAAGCTGGTACCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:1857094/140‑1 (MQ=255)

                                                             tggTGTATTTGAGGCTAAGGTAAGCTGGTAAATTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                          <  2:5241016/139‑1 (MQ=255)

                                                             aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                          <  1:1567404/140‑1 (MQ=255)

                                                              ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                                         >  2:687707/1‑140 (MQ=255)

                                                                tgtaTTTGTGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                       <  1:942021/137‑1 (MQ=255)

                                                                tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                       >  1:4151526/1‑140 (MQ=255)

                                                                tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                       >  2:1898172/1‑140 (MQ=255)

                                                                 gtaTTTGCGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga                                                                      <  1:2244254/138‑1 (MQ=255)

                                                                  gttttGAGGCTAAGTAATGCTGGTAACTTCTCTGTATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAATGCCACCAACCACAAACCAGACAAATTCCGAGCGAGaa                                                                     <  2:4820204/138‑1 (MQ=255)

                                                                  gATTTGTGGCTATGGTAAGCTGGTAACTTCTCTGTATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  1:3413185/140‑1 (MQ=255)

                                                                  gATTTGCGGCTATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  1:3088847/140‑1 (MQ=255)

                                                                  gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  2:6192747/140‑1 (MQ=255)

                                                                  gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  2:4367398/140‑1 (MQ=255)

                                                                  gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  1:3248008/140‑1 (MQ=255)

                                                                      tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACa                                                                 >  1:5852732/1‑140 (MQ=255)

                                                                        aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                               >  1:2790866/1‑140 (MQ=255)

                                                                              aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCa                                                         >  2:4567088/1‑140 (MQ=255)

                                                                               ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCaa                                                        <  2:2807381/140‑1 (MQ=255)

                                                                                        gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATATCAAAATATAAACAGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACCTATACCCAAAACTCGAAc                                               >  1:5041274/1‑140 (MQ=255)

                                                                                        gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                               >  2:2539249/1‑140 (MQ=255)

                                                                                          aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAt                                             >  2:2039141/1‑140 (MQ=255)

                                                                                            cTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt                                           <  1:6250402/140‑1 (MQ=255)

                                                                                              tctcTTTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  1:2044147/140‑1 (MQ=255)

                                                                                              tctcTGTCTATGAGAGCCGTTAACACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  2:1322333/140‑1 (MQ=255)

                                                                                              tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  1:2538676/140‑1 (MQ=255)

                                                                                              tctcGGTCGATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  2:2752391/140‑1 (MQ=255)

                                                                                                  tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAGTATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCa                                     >  1:368491/1‑140 (MQ=255)

                                                                                                   gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCag                                    >  2:3096279/1‑139 (MQ=255)

                                                                                                      tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa                                 <  1:5868318/140‑1 (MQ=255)

                                                                                                       aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAag                                >  1:3273589/1‑140 (MQ=255)

                                                                                                       aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAag                                >  1:2427381/1‑140 (MQ=255)

                                                                                                            agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGttt                           >  1:971249/1‑140 (MQ=255)

                                                                                                              ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                          >  2:1982782/1‑91 (MQ=255)

                                                                                                              ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:3442574/1‑140 (MQ=255)

                                                                                                              ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:5777196/1‑140 (MQ=255)

                                                                                                              ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:1226334/1‑140 (MQ=255)

                                                                                                              ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:4406531/1‑140 (MQ=255)

                                                                                                               cGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCt                        <  1:14065/140‑1 (MQ=255)

                                                                                                                gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                       <  1:2968317/140‑1 (MQ=255)

                                                                                                                gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                       >  2:2715370/1‑140 (MQ=255)

                                                                                                                gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                       >  1:833403/1‑140 (MQ=255)

                                                                                                                    acaCGACTCTCATAGATTTTACTAAGAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAGCATTTCCCGCATAAAGAGTTTTCTtaaga                   <  2:4018685/138‑1 (MQ=255)

                                                                                                                    aaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaaga                   <  1:1100472/140‑1 (MQ=255)

                                                                                                                     aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat                  <  1:1460067/140‑1 (MQ=255)

                                                                                                                     aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat                  <  2:4399107/140‑1 (MQ=255)

                                                                                                                      aCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata                 <  2:1163024/140‑1 (MQ=255)

                                                                                                                      aCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata                 <  2:2653145/140‑1 (MQ=255)

                                                                                                                      aCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAAGACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata                 <  1:1007167/140‑1 (MQ=255)

                                                                                                                       cGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa                <  1:1139561/140‑1 (MQ=255)

                                                                                                                       cGACTCTCATAGATTTGACTAATAGCAAAATATAAACCGTCCCCAAAAAACCCACCAACCACAAACCAGACAAATACCGAGCGAGAAGACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa                <  2:6136688/140‑1 (MQ=255)

                                                                                                                         aCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAACCCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga              <  2:1432854/140‑1 (MQ=255)

                                                                                                                         aCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga              >  2:3410323/1‑140 (MQ=255)

                                                                                                                           tctcATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaat            >  1:3699786/1‑140 (MQ=255)

                                                                                                                           tctcATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaat            <  1:717786/140‑1 (MQ=255)

                                                                                                                              cATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAACAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataat         <  1:6034246/140‑1 (MQ=255)

                                                                                                                              cATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACCAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataat         <  2:174193/140‑1 (MQ=255)

                                                                                                                              cATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataat         <  1:3873832/140‑1 (MQ=255)

                                                                                                                              cATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataat         >  1:542064/1‑140 (MQ=255)

                                                                                                                                 aGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAg      <  1:4557792/140‑1 (MQ=255)

CGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGC  >  NZ_CP025268/3174046‑3174318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`