BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,255,705	A→G	234 (TAA→TAG)	yhaK →	pirin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,255,705	0	A	G	99.4%	1059.5 / NA	308	234 (TAA→TAG)	yhaK	pirin family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (183/123); minor base T (1/1); total (184/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

AATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCT  >  NZ_CP025268/3255569‑3255841

      aTGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTa                                                                                                                                 <  1:2945834/140‑1 (MQ=255)

        gcgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACa                                                                                                                               <  1:5399316/140‑1 (MQ=255)

        gcgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACa                                                                                                                               <  1:2543433/140‑1 (MQ=255)

         cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                                                                              <  2:837927/140‑1 (MQ=255)

           cTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                                                                            <  2:6399215/140‑1 (MQ=255)

           cTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                                                                            <  2:2413833/140‑1 (MQ=255)

           cTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                                                                            <  1:3933122/140‑1 (MQ=255)

           cTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                                                                            <  2:5311535/140‑1 (MQ=255)

                    catGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatga                                                                                                                   >  1:131222/1‑140 (MQ=255)

                     atGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  <  2:3574556/140‑1 (MQ=255)

                     atGAAGAGAAAGCGGCGCTGACCTGCGGNGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  <  1:5669292/140‑1 (MQ=255)

                      tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgctgatg                                                                                                                 >  2:5107111/1‑140 (MQ=255)

                      tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgatgatg                                                                                                                 >  2:1687971/1‑140 (MQ=255)

                      tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGaagatgatg                                                                                                                 >  2:5651439/1‑140 (MQ=255)

                      tGAAGAGAAAGCGGCGATGTCCTGCGGTGCTGGGGCGTTAGTTCGGGACGAGGCTAGCATTACGCTGGTTGCCGAGTCCCCACTGCGCGCGTTGCTGATAGATTTGCCTGGCTGGTTGTTTTTACGGGGAGatgaggatg                                                                                                                 >  2:3419957/1‑140 (MQ=255)

                           agaAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaa                                                                                                            >  1:3663499/1‑140 (MQ=255)

                            gataGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTATCTTTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGGTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaa                                                                                                           >  1:876736/4‑140 (MQ=255)

                            gaAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaa                                                                                                           >  1:4758818/1‑140 (MQ=255)

                             aaaGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCGGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaa                                                                                                          >  2:5560495/1‑140 (MQ=255)

                              aaGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaaa                                                                                                         >  2:4034909/1‑140 (MQ=255)

                              aaGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaaa                                                                                                         >  1:1517491/1‑140 (MQ=255)

                               aGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaaaa                                                                                                        >  1:3239544/1‑140 (MQ=255)

                                             gCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcg                                                                                          <  2:719152/140‑1 (MQ=255)

                                             gCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACGGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcg                                                                                          <  2:6136517/140‑1 (MQ=255)

                                              cGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAACTTGGCCAAAAAgcgc                                                                                         >  2:2950613/1‑140 (MQ=255)

                                              cGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCGGTCTAGTTGTTTTTACAGGGAGAGGATGATGAGTAAAAAATTGGCCAAAAAgcgc                                                                                         >  1:4450542/1‑140 (MQ=255)

                                               ggTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGcc                                                                                        <  1:5964612/140‑1 (MQ=255)

                                                 tGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAg                                                                                      <  1:3913623/140‑1 (MQ=255)

                                                  gATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGc                                                                                     <  1:2787234/140‑1 (MQ=255)

                                                   aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGcc                                                                                    >  2:5070975/1‑140 (MQ=255)

                                                   aTGGGGCGGTTATTCGTGCCGAGGCTAACAGTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCGGATAGATTTGCCTGTCTCGTTGTTTTTACAGGGAGATGATGCTGAGGAAAAAATTGGCCGAAATGCGCCAGcc                                                                                    >  1:3975319/1‑140 (MQ=255)

                                                      gggCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGt                                                                                 <  1:6457560/140‑1 (MQ=255)

                                                       ggCGTTTATTCGTGACGAGGCTAACAGTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGAGGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTg                                                                                <  1:4213729/140‑1 (MQ=255)

                                                        gCGTTTCTTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAATAGCGCCAGCCGGTGa                                                                               >  1:2702795/1‑140 (MQ=255)

                                                        gCGTTTCGTCGTGACGAGGCTAACATTACGCTGGGTGCCGATTCCCCACTGCGCGCTTTGCTGTGAGATTTGCCTGTCTCGTTGTTTGTACTGGGAGAGGATGAGGAGTAAAACATGGGCCAAAAAGCGCCCGCCGGTGa                                                                               >  2:1268120/1‑140 (MQ=255)

                                                        gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGa                                                                               >  1:261754/1‑140 (MQ=255)

                                                           tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                                                                            >  1:1751339/1‑140 (MQ=255)

                                                           tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                                                                            <  1:6022621/140‑1 (MQ=255)

                                                                 cGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGTAGCCCgtgg                                                                      >  2:1860952/1‑140 (MQ=255)

                                                                 cGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtgg                                                                      >  1:5607649/1‑140 (MQ=255)

                                                                   tGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATTAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggtg                                                                    <  2:2299533/140‑1 (MQ=255)

                                                                   cgccgccgCTACCAGTACGCTTGTTGCCGATACCCCACTGCGCGCTTGGCTGATCGCCGTGCCTGTCTAGTTGTTTTTACAGGCAGAAGATTCCGAGTAAACCATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggtg                                                                    <  2:281817/133‑1 (MQ=255)

                                                                       gAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGa                                                                <  1:1131791/140‑1 (MQ=255)

                                                                        aGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGaa                                                               >  1:1556751/1‑140 (MQ=255)

                                                                         ggCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGaaa                                                              >  1:3758421/1‑140 (MQ=255)

                                                                          gCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAg                                                             <  1:2664056/140‑1 (MQ=255)

                                                                          gCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCGGATAGATTTGCCTGTCTAGTGGTTGTTACTGGGAGATGATGATGAGTAAAACATTGGCCACAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGaagg                                                             >  2:142690/1‑138 (MQ=255)

                                                                            tAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGaa                                                           >  1:104131/1‑140 (MQ=255)

                                                                                 ttACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAATAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGc                                                      >  1:3541464/1‑140 (MQ=255)

                                                                                    cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGCTGATGATGAGTACAAAATTGGCCACAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGCAAGACCCTGCTCg                                                   >  2:1493361/1‑140 (MQ=255)

                                                                                    cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:3259889/1‑140 (MQ=255)

                                                                                    cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:1779987/1‑140 (MQ=255)

                                                                                    cGCTGGTTGCCGATTCCCAACTGCGCGCTTTGCTGATAGATTTGCCTGTGTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAATTTGGCCAAAAAGCGCCAGCCGGTGAAGCCAGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:4449242/1‑140 (MQ=255)

                                                                                     gCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGc                                                  <  1:423455/140‑1 (MQ=255)

                                                                                      cTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAACGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCa                                                 <  1:1424651/140‑1 (MQ=255)

                                                                                      cTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCa                                                 <  2:2009916/140‑1 (MQ=255)

                                                                                       tGGTTGCCGAGTCCCCCCGGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCAGCTCGctc                                                <  1:6115919/140‑3 (MQ=255)

                                                                                        ggTTGCCGATTCCCCACTGCGCGCTTAGCTGATAGATTTGCCAGTCGAGTTGTTTTTACAGGGAGTTGATGTGGAGTCAAAACTTGGCCAAAAAGCGCCAGCCGGTGACGCCCGTGGTGGCGAAAGAACCTGGTCGCAcc                                               >  2:1117630/1‑140 (MQ=255)

                                                                                         gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  1:6048010/1‑140 (MQ=255)

                                                                                          ttGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGc                                             >  2:31098/1‑140 (MQ=255)

                                                                                                 ttCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAAt                                      >  1:520756/1‑140 (MQ=255)

                                                                                                   ccccACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCATAAAttt                                    >  2:3222972/1‑140 (MQ=255)

                                                                                                     ccACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTg                                  <  1:2845511/140‑1 (MQ=255)

                                                                                                       ccTGCGCGCTTTGCTGATAGATTTGCCTGGCTAGTGGTTTTTACAGGGAGCTGATGATGAGTCAAAAATAGGCCAAAAAGCGCCAGCCGGTGAAGCACGTGGTGGCGAAAGAACCTGCTCGCACCGCCCAAAAtgtatgc                                >  2:4521467/2‑134 (MQ=255)

                                                                                                       aCTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGc                                >  1:207015/1‑140 (MQ=255)

                                                                                                       aCTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGc                                >  1:489215/1‑140 (MQ=255)

                                                                                                        cTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCt                               <  2:1534961/140‑1 (MQ=255)

                                                                                                         tGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTa                              <  2:3729500/140‑1 (MQ=255)

                                                                                                          gcgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAt                             >  2:5210198/1‑140 (MQ=255)

                                                                                                          gcgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAt                             >  1:5039867/1‑140 (MQ=255)

                                                                                                           cgcgcTTTTCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGTTGATGAGTAAAAAATTGGCAAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            <  2:3832351/140‑1 (MQ=255)

                                                                                                           cgcgcTTTGCTGATTGATTTGCCTGTCTAGTTGTGTTTCCAGGGAGATGATGAGGAGTACAACATTGGGCAAAATGCGCCAGCCGGTGACGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:4015573/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTNCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAANGAACCTGNTCGCACCGCCAAAAATTTTGGCTATg                            >  1:5633733/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTATTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGACAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:243097/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTCAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:1990793/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGACGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTagg                            >  2:4612343/1‑138 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:1223157/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:6287310/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:5933537/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:772361/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:4414768/1‑140 (MQ=255)

                                                                                                           cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:4285792/1‑140 (MQ=255)

                                                                                                           cgcgcTTAGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:1775852/1‑140 (MQ=255)

                                                                                                            gcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGa                           >  1:5586912/1‑140 (MQ=255)

                                                                                                            gcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATATTGGCTATGa                           >  2:1020068/1‑140 (MQ=255)

                                                                                                             cgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGaa                          <  1:3357652/140‑1 (MQ=255)

                                                                                                                tttGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAagag                       <  1:2277248/140‑1 (MQ=255)

                                                                                                                tttGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAagag                       >  1:4602645/1‑140 (MQ=255)

                                                                                                                   gCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGa                                                                <  2:4732024/96‑1 (MQ=255)

                                                                                                                        tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:4121460/140‑1 (MQ=255)

                                                                                                                          gATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTACAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcg             >  1:2894214/1‑140 (MQ=255)

                                                                                                                          gATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcg             >  2:1477402/1‑140 (MQ=255)

                                                                                                                          gATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcg             >  1:1611683/1‑140 (MQ=255)

                                                                                                                            tttGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcgag           >  2:4919769/1‑140 (MQ=255)

                                                                                                                            tttGCCTGTCGAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcgag           <  2:5925464/140‑1 (MQ=255)

                                                                                                                              tGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCt         >  2:217252/1‑140 (MQ=255)

                                                                                                                               gCCTGTCTAGTTGTTTTTAGAGGGAGATGATGATGAGTAAAATATTGCACAAAATGCGCCAGCCGGTTATGCACGTTGTGGAGAAAGAACCTGCTAGCTCCGAGAAAAATTTTGGCTTTGAAGAGATTTTGATCGATCTg        <  2:3323810/140‑1 (MQ=255)

                                                                                                                                    tCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGc   >  1:2391937/1‑140 (MQ=255)

                                                                                                                                    tCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGc   >  1:5116053/1‑140 (MQ=255)

                                                                                                                                     cTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCt  <  1:1371810/140‑1 (MQ=255)

AATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCT  >  NZ_CP025268/3255569‑3255841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`