BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,258,360	A→G	G112G (GGT→GGC)	cyuP ←	amino acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,258,360	0	A	G	99.7%	1072.3 / NA	310	G112G (GGT→GGC)	cyuP	amino acid permease
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (155/153); minor base T (0/1); total (155/155)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

TAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAAT  >  NZ_CP025268/3258223‑3258495

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                       >  2:4455366/1‑140 (MQ=255)

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                       >  2:1111454/1‑140 (MQ=255)

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                       >  2:3981610/1‑140 (MQ=255)

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                       >  2:1091011/1‑140 (MQ=255)

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                       >  2:41005/1‑140 (MQ=255)

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                       >  2:2366934/1‑140 (MQ=255)

 aatatgtaCTTTCTGACATCTACCCTTCCGTCAAGCCTTAGGTATGCATGTAGGAAGAACTATCGTTGGTGATGGCGGTGGAATATACGAACATCCTAATCACCATCATTACGAAATTGAGCGCTCCTAATATGATGccc                                                                                                                                      <  2:5269478/133‑1 (MQ=255)

      ggACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGtt                                                                                                                                 >  1:154664/1‑140 (MQ=255)

         cTGTCTGACAGCACCCCTTCCGTCACGCCCAAGGTATGCAGGTCGGAAGCACTATCGTTGGAGACGGCGGTGGAATAGACGAACATCCACCTCACCAGCATTACGAAATAGAGCGCACCTACCAGGATCCCCCAGttttg                                                                                                                              <  2:2345384/140‑2 (MQ=255)

         cTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTCGGAAGCACTATCGTTGGAGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGAGCACCTAACAGGATGCCCCAGttttt                                                                                                                              >  2:2121842/1‑140 (MQ=255)

          tGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTa                                                                                                                             >  2:1612208/1‑140 (MQ=255)

          tGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACAAGCATTACGAAAGAGAGCGCACCTAACAGGATGCCCCAGTTTTTa                                                                                                                             >  2:5789109/1‑140 (MQ=255)

           gTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAc                                                                                                                            >  1:752060/1‑140 (MQ=255)

           gTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAc                                                                                                                            <  1:3376408/140‑1 (MQ=255)

            tCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAcc                                                                                                                           >  1:399004/1‑140 (MQ=255)

            tCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAcc                                                                                                                           >  1:4912638/1‑140 (MQ=255)

            tCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACGATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAcc                                                                                                                           <  1:1263729/140‑1 (MQ=255)

                 cAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAAt                                                                                                                      <  1:4335290/140‑1 (MQ=255)

                 cAGCAACCCATCCGTCACGCCGAAGGTATTCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCTCACCTAACAGTATTCCCCAGTATTTACCTAAAt                                                                                                                      <  2:2156060/140‑1 (MQ=255)

                 cAGCAACCCATCCGTCACGCCGAAGGTAAGCACGTAGGAAGCACTAACGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTACCAGGATGCCCCAGTTTTTACCTAAAt                                                                                                                      <  2:4690200/140‑1 (MQ=255)

                  aGCACCCCTTCCGTCACGCCGAAGGTCTGCAGGTAGGAAGCACTATCGATGGTGATGGCGGTGGAATAGACGAACATCCACAACACCAGCATTACGCAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTACATa                                                                                                                     <  2:2738008/140‑1 (MQ=255)

                  aGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATATACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATa                                                                                                                     <  2:2898551/140‑1 (MQ=255)

                  aGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAAAAGACGAACATCCAAAGCACCCGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAACTa                                                                                                                     <  2:6170887/140‑1 (MQ=255)

                     aaCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAAcc                                                                                                                  <  2:2545416/140‑1 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGATATAGAGCGCACCTAACAGGAGGCCCCAGTTTTTACCTAAATAACCg                                                                                                                 >  1:3158191/1‑140 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                 >  1:2889325/1‑140 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                 >  2:1748610/1‑140 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                 <  1:6117734/140‑1 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                 >  2:4631432/1‑140 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                 >  1:5284675/1‑140 (MQ=255)

                               tCACGCCGAAGGTATGCCGGTAGGAAGCACTAACGTTGGTGATGGCGGTGGATTAGACGCACATCCAAAACCCCAGCAATACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCCCTACTGACg                                                                                                        <  2:1793148/140‑1 (MQ=255)

                               tCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                        >  2:2261263/1‑140 (MQ=255)

                               tCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                        <  1:1404770/140‑1 (MQ=255)

                               tCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                                        <  1:4187876/140‑1 (MQ=255)

                                cACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAACTCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGc                                                                                                       <  1:5408243/140‑1 (MQ=255)

                                 aCGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCt                                                                                                      <  1:1240962/140‑1 (MQ=255)

                                    ccGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCgg                                                                                                   <  2:895341/140‑1 (MQ=255)

                                      gAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGTACATCCAAATCACCAGCATTACGTAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                 <  2:2068443/140‑1 (MQ=255)

                                      gAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                 <  2:3336406/140‑1 (MQ=255)

                                      gAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                 <  1:5142958/140‑1 (MQ=255)

                                      gAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                 <  1:809123/140‑1 (MQ=255)

                                       aaGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTa                                                                                                >  1:5177221/1‑140 (MQ=255)

                                        aGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTaa                                                                                               <  1:197347/140‑1 (MQ=255)

                                           tATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                                                                            <  1:5847887/140‑1 (MQ=255)

                                           tATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                                                                            <  2:4906922/140‑1 (MQ=255)

                                                    aGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCt                                                                                   >  2:49971/1‑140 (MQ=255)

                                                    aGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCt                                                                                   >  2:3467179/1‑140 (MQ=255)

                                                    aGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCt                                                                                   >  2:2459498/1‑140 (MQ=255)

                                                     ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTCATCTTTACATTCTg                                                                                  >  2:5058954/1‑140 (MQ=255)

                                                     ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                  >  2:3246796/1‑140 (MQ=255)

                                                     ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                  <  1:1221543/140‑1 (MQ=255)

                                                     cgAAGCACTATCGTTGGTGATGGCTGTGGAATAGACGAACATCCAAATCACCAGCAATACCAAATACAGCGCACCTAACACGATGCCCCAGAATTTACCAAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                  <  2:5575286/139‑1 (MQ=255)

                                                            cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTc                                                                           >  2:5251645/1‑140 (MQ=255)

                                                             tATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCt                                                                          >  1:181120/1‑140 (MQ=255)

                                                               tCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                        <  1:251807/140‑1 (MQ=255)

                                                                  ttGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAg                                                                     >  2:3687130/1‑140 (MQ=255)

                                                                  atGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAg                                                                     <  2:6106308/139‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAGCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                   <  1:1389854/140‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                   <  1:201575/140‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                   <  1:6323889/140‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                   <  1:5594083/140‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACANCCAAATCNCCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACNTTCTGGTGATTCTGCCAGCg                                                                   <  1:5630813/140‑1 (MQ=255)

                                                                         tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATta                                                              >  2:5319321/1‑140 (MQ=255)

                                                                                ggAATAGTAGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCGCAGATTTTACCTAAATAACCGATAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACa                                                       <  2:2505358/140‑1 (MQ=255)

                                                                                  aaTAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAc                                                                                                                            >  1:2257401/1‑69 (MQ=255)

                                                                                      gACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCt                                                 <  2:387907/140‑1 (MQ=255)

                                                                                      gACGAACATCAAAATCACCAGCATTACGAAATAGAGAGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGTTGATTCTGCCAGCGTATTAATAAACAATCGCt                                                 <  1:2451507/140‑1 (MQ=255)

                                                                                       aCGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTg                                                >  1:3597855/1‑140 (MQ=255)

                                                                                        cGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCGAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGa                                               <  2:4342703/140‑1 (MQ=255)

                                                                                           aCATCCAAATCTCCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                            <  2:3013169/140‑1 (MQ=255)

                                                                                           aCATCCAAATCCCCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                            <  1:1070995/140‑1 (MQ=255)

                                                                                           aCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                            >  1:1008757/1‑140 (MQ=255)

                                                                                           aCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                            >  2:1022829/1‑140 (MQ=255)

                                                                                            cATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACa                                           >  1:1755331/1‑140 (MQ=255)

                                                                                              tCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGGTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGa                                         >  2:4760255/1‑140 (MQ=255)

                                                                                               ccAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGAt                                        >  2:3676217/1‑140 (MQ=255)

                                                                                                   aTCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACAt                                    <  1:6057260/140‑1 (MQ=255)

                                                                                                    tCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACAtt                                   <  2:5536607/140‑1 (MQ=255)

                                                                                                    tCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACAtt                                   <  1:6415994/140‑1 (MQ=255)

                                                                                                       ccAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGcc                                >  2:825648/1‑140 (MQ=255)

                                                                                                       ccAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGcc                                >  1:3659150/1‑140 (MQ=255)

                                                                                                        cAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCg                               >  1:4999118/1‑140 (MQ=255)

                                                                                                          gCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCggg                             <  1:1102668/140‑1 (MQ=255)

                                                                                                          gCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCggg                             >  1:377877/1‑140 (MQ=255)

                                                                                                          gCAGTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGTGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCggg                             <  2:2198324/140‑1 (MQ=255)

                                                                                                           cagtACGAAATAGAGCGCACCGAACCGGATGCCCCAGTTTTTACCTAAATCACCGCTAATGACGCTCGGGTAATCTTTACATTCGGGTGATTCTGCCAGCGTATTCATAAACAATCGCTGACACAGATACATTGCCGGGt                            <  2:2274868/137‑1 (MQ=255)

                                                                                                            aTTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAGTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTa                           <  1:267423/140‑1 (MQ=255)

                                                                                                                cGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCa                       >  1:3493557/1‑140 (MQ=255)

                                                                                                                cGAAATAGAGCGCACCTAACAGGATGCCACAGTTTTTACCTAAATAAACGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGACGGGTAACCa                       >  2:3543858/1‑140 (MQ=255)

                                                                                                                 gcaaTAGAGCGCCCCCAACAGGATGCCCCAGTTTTTACCAAAAAAACCGCTAATCACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCGGAAACAGATACATTGCCGGGAAACCaa                      <  2:3934860/138‑1 (MQ=255)

                                                                                                                  aaaTAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAAt                     >  1:5780326/1‑140 (MQ=255)

                                                                                                                     tAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCAc                  >  1:4716641/1‑140 (MQ=255)

                                                                                                                     tAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCAc                  >  2:5945730/1‑140 (MQ=255)

                                                                                                                         gcgcACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGAt              <  1:2478073/140‑1 (MQ=255)

                                                                                                                          cgcACCTAACAGGATGCCCCAGTTTTTACCTAAATa                                                                                                                     <  2:1435165/36‑1 (MQ=255)

                                                                                                                              ccTAACAGGATGCCCCAGTTTTTACCTAAATAACCGATAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGagag         <  2:5620412/140‑1 (MQ=255)

                                                                                                                               cTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGc        >  2:1254201/1‑140 (MQ=255)

                                                                                                                                 aaCAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaa      <  2:2145395/140‑1 (MQ=255)

                                                                                                                                     ggATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAAt  <  1:3319898/140‑1 (MQ=255)

                                                                                                                                     ggATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAAt  <  1:2104936/140‑1 (MQ=255)

TAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAAT  >  NZ_CP025268/3258223‑3258495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`