BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,386,542	T→C	91 (TAA→TAG)	yhcO ←	barstar family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,386,542	0	T	C	99.4%	572.2 / NA	166	91 (TAA→TAG)	yhcO	barstar family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (97/68); minor base A (1/0); total (98/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

AGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCAGGA  >  NZ_CP025268/3386411‑3386678

aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACg                                                                                                                                  <  2:6013496/140‑1 (MQ=255)

  gcgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGaa                                                                                                                                <  1:4628359/140‑1 (MQ=255)

     caccGGCCTACCAGATGACTGATGCTCGTAGCGGTGACACCTGGCTCGCTACGGCTGAACTGTACAAAGATACCCTCATCGTCTTGTCGGTCGCTATTGCCCCCGGTTCGGGGGCTTTTTTGTGCGCTAATGACGACCag                                                                                                                             >  2:2789147/4‑139 (MQ=255)

        cGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaa                                                                                                                          <  2:3407653/140‑1 (MQ=255)

        cGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaa                                                                                                                          >  1:183126/1‑140 (MQ=255)

        cGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaa                                                                                                                          >  2:2001048/1‑140 (MQ=255)

          gCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaaaa                                                                                                                        <  2:5996807/140‑1 (MQ=255)

          gCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaaaa                                                                                                                        <  1:6031260/140‑1 (MQ=255)

            cTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACg                                                                                                                      >  2:524642/1‑140 (MQ=255)

                   aTTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGc                                                                                                               <  2:3198748/140‑1 (MQ=255)

                   aTTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGc                                                                                                               <  1:5095286/140‑1 (MQ=255)

                   aTTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGc                                                                                                               <  1:2141356/140‑1 (MQ=255)

                     tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                             >  2:1623323/1‑140 (MQ=255)

                     tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                             >  2:1357443/1‑140 (MQ=255)

                       cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                                           >  2:1847544/1‑140 (MQ=255)

                       cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                                           >  2:5694486/1‑140 (MQ=255)

                       cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                                           >  1:1279941/1‑140 (MQ=255)

                       cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                                           >  1:25817/1‑140 (MQ=255)

                             cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                     <  2:3278295/140‑1 (MQ=255)

                             cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                     >  1:4308576/1‑140 (MQ=255)

                              tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctc                                                                                                    <  1:696638/140‑1 (MQ=255)

                               cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct                                                                                                   >  1:960158/1‑140 (MQ=255)

                               cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct                                                                                                   <  1:3089861/140‑1 (MQ=255)

                                 tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                                 <  2:149439/140‑1 (MQ=255)

                                 tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                                 >  1:4546991/1‑140 (MQ=255)

                                      gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                            <  1:675535/140‑1 (MQ=255)

                                      gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                            >  1:4766840/1‑140 (MQ=255)

                                      gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                            >  2:25108/1‑140 (MQ=255)

                                      gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                            >  1:109919/1‑140 (MQ=255)

                                           acCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtca                                                                                       <  1:2801102/140‑1 (MQ=255)

                                            ccTGGCACGCTACGGCTGTACTGGACAAATACACCCACATCGTCTTGTCCGTCGATGTTGCCCCCCGTGCGGGTGCTTTTGTTTGCGCTAAAGAGGAACAGTCTCACGCAAAGGCCGTGCCAGGTCGTCCTCTGCCtccg                                                                                      >  2:6413210/1‑138 (MQ=255)

                                              tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                    >  2:4716789/1‑140 (MQ=255)

                                              tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                    <  1:5891701/140‑1 (MQ=255)

                                                gCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatcata                                                                                  >  1:4451483/1‑138 (MQ=255)

                                                 cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTGGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTGTTGCGCTAATGACGAACAGTAAAACGCTAATGCCCTTCCAGCTCTTCCTCGGCCTCCTCacac                                                                                 >  2:5866515/1‑140 (MQ=255)

                                                  acggttcGGCTGTAATTTACAAATAAACCCTCATCGTCTTGTCCGTAGATATTGCCCCCGGTTCGGGGGGTTTTTTTTTCGCTAATGTCGAAAATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACa                                                                                <  1:3911574/134‑1 (MQ=255)

                                                   cGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                               <  1:4962277/140‑1 (MQ=255)

                                                   cGCTACGGCTGAACTGTACAAAGAAACCCTCATCGTCTTGTCCGACGATTTTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTTATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                               <  1:4199947/140‑1 (MQ=255)

                                                     cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTACAGCTCATCCTCTGCCTCATCAAACAGCa                                                                             >  2:5488810/1‑140 (MQ=255)

                                                      tACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACg                                                                                                                                  >  1:2912309/1‑86 (MQ=255)

                                                      tACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAATCGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCaa                                                                            >  2:2334775/1‑140 (MQ=255)

                                                            tGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTaa                                                                      <  1:825641/140‑1 (MQ=255)

                                                              aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                                                    >  2:5656882/1‑140 (MQ=255)

                                                              aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                                                    >  1:1063100/1‑140 (MQ=255)

                                                              aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                                                    <  1:1867550/140‑1 (MQ=255)

                                                                  gTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTACCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGcc                                                                >  1:2589632/1‑140 (MQ=255)

                                                                   tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                               >  2:5973122/1‑140 (MQ=255)

                                                                   tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                               >  1:1807049/1‑140 (MQ=255)

                                                                   tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGAAATATTAACGCGCCa                                                               >  1:4916862/1‑140 (MQ=255)

                                                                   tACAAATAAACCCACATCGACTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTATTTTTTGCGCTAATGACGAACAATAAAACGCAAAAGCCCATCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                               >  1:4643280/1‑140 (MQ=255)

                                                                     cAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCaaa                                                             >  2:3272121/1‑140 (MQ=255)

                                                                         tAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACgg                                                         <  1:2922420/140‑1 (MQ=255)

                                                                             cccTCATCGTCTTGTCCGACGATATTTCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCACACAGCAATATTAACGCGCCAAAACGGCGAc                                                     >  2:2121005/1‑140 (MQ=255)

                                                                             cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                    >  1:1200394/1‑109 (MQ=255)

                                                                             cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                    <  2:1200394/109‑1 (MQ=255)

                                                                             cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                     >  1:4378287/1‑140 (MQ=255)

                                                                             cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                     >  1:6382133/1‑140 (MQ=255)

                                                                                  aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                                                >  1:2728025/1‑140 (MQ=255)

                                                                                    cGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                              <  1:518200/140‑1 (MQ=255)

                                                                                     gTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtc                                             <  1:3438407/140‑1 (MQ=255)

                                                                                       cTTGTCCGACGATATTGCCCNCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctc                                           >  1:5637442/1‑140 (MQ=255)

                                                                                        ttGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                          >  1:5282546/1‑140 (MQ=255)

                                                                                        ttGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                          <  1:1671688/140‑1 (MQ=255)

                                                                                        ttGTCCGACGATATTGCCCCCGGTTCGGGGGCTATTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                          <  1:6307576/140‑1 (MQ=255)

                                                                                          gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACAGCGACGCGTTTTCTCTcc                                        >  1:823338/1‑140 (MQ=255)

                                                                                              gacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                          <  1:3265471/134‑1 (MQ=255)

                                                                                              gacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGa                                    >  2:6141578/1‑140 (MQ=255)

                                                                                                cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATg                                                                                                                                     <  2:2939127/41‑1 (MQ=255)

                                                                                                cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg                                  <  1:4828443/140‑1 (MQ=255)

                                                                                                cgaTATTGCCCCCAGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg                                  >  1:6154224/1‑140 (MQ=255)

                                                                                                 gaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGa                                 <  1:4317267/140‑1 (MQ=255)

                                                                                                    atTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACa                              >  1:3538394/1‑140 (MQ=255)

                                                                                                    atTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACa                              <  2:6272475/140‑1 (MQ=255)

                                                                                                    atTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACa                              >  1:2522361/1‑140 (MQ=255)

                                                                                                     ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAATCGCACATGCCCTTCCAGCTCTTCCTCTGCCTGGTCAAACAGCAATATTATGGCGCCCTAACGGCGTCGCGGTGGCTCTCCCAGATGTACaa                             >  2:5580759/1‑140 (MQ=255)

                                                                                                     ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACaa                             >  1:230395/1‑140 (MQ=255)

                                                                                                     ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACaa                             >  1:3329405/1‑140 (MQ=255)

                                                                                                     ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCGTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACaa                             >  1:2029089/1‑140 (MQ=255)

                                                                                                        cccccGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                                          >  2:1103855/1‑60 (MQ=255)

                                                                                                        cccccGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAAtt                          <  1:575022/140‑1 (MQ=255)

                                                                                                         ccccGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTc                         <  1:2001048/140‑1 (MQ=255)

                                                                                                           ccGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa                       <  1:1479920/140‑1 (MQ=255)

                                                                                                           ccGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa                       >  1:110897/1‑140 (MQ=255)

                                                                                                           ccGGTTCGGGGGCATTTTTTTGCGCTAATGACGAACATTAAAACACAAATGCCCTTCCAGCTCTTCCTCTGCATCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCACAGATGAACAAATTCaa                       >  1:5717262/1‑140 (MQ=255)

                                                                                                               ttCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtctc                   <  2:4118534/140‑1 (MQ=255)

                                                                                                               ttCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtctc                   <  2:5637442/140‑1 (MQ=255)

                                                                                                                tCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCa                  >  1:5305521/1‑140 (MQ=255)

                                                                                                                 cGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCaa                 <  2:999352/140‑1 (MQ=255)

                                                                                                                 cGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCaa                 <  2:5787536/140‑1 (MQ=255)

                                                                                                                  gggggCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAg                >  1:5164644/1‑140 (MQ=255)

                                                                                                                      gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt            <  2:340723/140‑1 (MQ=255)

                                                                                                                      gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt            <  1:1738676/140‑1 (MQ=255)

                                                                                                                      gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt            <  2:817665/140‑1 (MQ=255)

                                                                                                                      gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt            <  1:1178508/140‑1 (MQ=255)

                                                                                                                       cTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTa           >  1:2410183/1‑140 (MQ=255)

                                                                                                                        ttttttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGCAg          <  2:109919/140‑1 (MQ=255)

                                                                                                                           tttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCACAAGTGGTAGCgg       <  1:5656882/140‑1 (MQ=255)

                                                                                                                              ttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCAg    >  2:2598907/1‑140 (MQ=255)

                                                                                                                                gcgcTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCAGGa  <  1:386319/140‑1 (MQ=255)

                                                                                                                                gcgcTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCAGGa  <  1:1617831/140‑1 (MQ=255)

AGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCAGGA  >  NZ_CP025268/3386411‑3386678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`