BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,434,451	A→G	T26A (ACA→GCA)	def →	peptide deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,434,451	0	A	G	99.8%	1491.0 / NA	428	T26A (ACA→GCA)	def	peptide deformylase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (185/242); minor base T (0/1); total (185/243)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 428 total aligned reads displayed.

GGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATACAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGC  >  NZ_CP025268/3434314‑3434586

ggggATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCa                                                                                                                                       >  2:4877798/1‑140 (MQ=255)

ggggATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCa                                                                                                                                       >  1:3458358/1‑140 (MQ=255)

ggggATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCa                                                                                                                                       >  1:5194129/1‑140 (MQ=255)

  ggATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGa                                                                                                                                     >  1:2624726/1‑140 (MQ=255)

   gATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGaa                                                                                                                                    <  2:3310740/140‑1 (MQ=255)

      ttGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAAtt                                                                                                                                 <  1:6103629/140‑1 (MQ=255)

        gTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCACAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCa                                                                                                                               >  2:2373036/1‑140 (MQ=255)

        gTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCa                                                                                                                               >  1:416449/1‑140 (MQ=255)

        gTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCa                                                                                                                               >  2:3559226/1‑140 (MQ=255)

          cTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGc                                                                                                                             >  2:3019540/1‑140 (MQ=255)

          cTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGc                                                                                                                             <  1:254322/140‑1 (MQ=255)

            agaatagaaGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGt                                                                                                                           <  1:2055142/140‑1 (MQ=255)

             gaatagaaGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTa                                                                                                                          <  2:2458475/140‑1 (MQ=255)

              aatagaaGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTACACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAt                                                                                                                         >  1:1434040/1‑140 (MQ=255)

                  gaagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcgtc                                                                                                                     <  2:2034451/140‑1 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTTAACCGGTAGTAGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  1:2685473/1‑140 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  1:5835313/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               >  1:1745340/1‑140 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  1:4490444/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               >  1:4328147/1‑140 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  2:2518275/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  2:2697115/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTAAATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               >  2:437036/1‑140 (MQ=255)

                        aataacattCAAACTCCACAACACATCTCAGGAGATTTAAGACAGTATTCCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCACCGTAACGTCgatcat                                                                                                               >  2:5403676/8‑140 (MQ=255)

                                tCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCga                                                                                                       >  1:5512878/1‑140 (MQ=255)

                                tCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCga                                                                                                       >  2:1819827/1‑140 (MQ=255)

                                 cccccTCCGGAACACACCACTGGAGAATCAGGTCCGTAGTCCAAGTGTTCCATCTCCCGGCCGAGCGCCATCGCAAAGTTGCTACACCGGTAGAAGAAGTGAATTCATAACTTCCGCGCATCGTAGATGCTAGGTTCgag                                                                                                      <  2:147036/136‑1 (MQ=255)

                                 cTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCgag                                                                                                      <  2:2126472/140‑1 (MQ=255)

                                  tAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCgaga                                                                                                     <  2:6419403/140‑1 (MQ=255)

                                  tAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCgaga                                                                                                     <  1:3183638/140‑1 (MQ=255)

                                   aaCTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGAc                                                                                                    <  2:5816604/140‑1 (MQ=255)

                                       ccTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATg                                                                                                >  1:2102351/1‑140 (MQ=255)

                                          gAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTAc                                                                                             <  1:4810777/140‑1 (MQ=255)

                                          gAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTAc                                                                                             <  1:245043/140‑1 (MQ=255)

                                           aaCACATCTCTGGAGATTTCTGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTGGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACg                                                                                            <  2:2270635/140‑1 (MQ=255)

                                           aaCACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACg                                                                                            <  1:5588695/140‑1 (MQ=255)

                                             cacaTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCa                                                                                          <  1:4693345/140‑1 (MQ=255)

                                             cacaTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCa                                                                                          <  1:3019540/140‑1 (MQ=255)

                                                aTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaa                                                                                       >  1:4979907/1‑140 (MQ=255)

                                                aTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaa                                                                                       >  1:5338900/1‑140 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTCAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     >  2:3166955/1‑140 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGCAGTGACTGCAGAAATTCCGCGTATCGTCGATGATATGTTCGAGACGATGTCCGCagaaga                                                                                     <  2:5577597/140‑1 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     >  1:117899/1‑140 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     >  2:1350279/1‑140 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     <  2:1725814/140‑1 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     <  2:762174/140‑1 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     >  1:3916578/1‑140 (MQ=255)

                                                     tGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAgg                                                                                  >  1:6026682/1‑140 (MQ=255)

                                                            ttATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGc                                                                           >  1:1830013/1‑140 (MQ=255)

                                                            ttATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGc                                                                           >  1:978414/1‑140 (MQ=255)

                                                            ttATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTAGAGACGATGTACGCAGAAGAAGGTATTGGc                                                                           >  2:1119839/1‑140 (MQ=255)

                                                              aTGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCt                                                                         <  1:1059913/140‑1 (MQ=255)

                                                                 tCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggc                                                                      <  2:5773244/140‑1 (MQ=255)

                                                                   aGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGACGAAGGTATTGGCCGggcgg                                                                    >  2:6145194/1‑140 (MQ=255)

                                                                   aGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcgg                                                                    <  1:6333220/140‑1 (MQ=255)

                                                                   aGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcgg                                                                    <  1:5111003/140‑1 (MQ=255)

                                                                   aGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcgg                                                                    <  1:6234710/140‑1 (MQ=255)

                                                                    gTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcg                                                                     >  1:5957224/1‑138 (MQ=255)

                                                                     ttttGCAAGTGTTACATATTCCGGACGTGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGTCGATGTACGCAGACGAAGGTATTGGCCTggcggct                                                                  >  2:5481880/1‑139 (MQ=255)

                                                                     ttttGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCa                                                                  >  1:2784079/1‑140 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGCGCGGCTTCGCAAAGTAGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  2:6069857/140‑1 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  2:4125735/140‑1 (MQ=255)

                                                                         gCAAGTGTGACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAATCCGGGAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAccc                                                              <  2:2853473/140‑1 (MQ=255)

                                                                               gtTACATATTCCGGACGAGCGTCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGAGAATGCAGAAAATCAGCGAATCGTCGATGATATGTTCGAGACGATGTAAGCAGAAGAATGTATTTGCCTGGCGGCAACCCACGTTg                                                        >  2:5196361/1‑140 (MQ=255)

                                                                               gtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGTGACGATGTACGCAGAAGTAGGTATTGGCCTGGCGGCAACCCAGGTTg                                                        >  1:1335927/1‑140 (MQ=255)

                                                                                  aCATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGata                                                     >  2:5546445/1‑140 (MQ=255)

                                                                                   cATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGatat                                                    <  1:3757149/140‑1 (MQ=255)

                                                                                       ttCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCAt                                                <  2:3893785/140‑1 (MQ=255)

                                                                                       ttCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCAt                                                <  2:886673/140‑1 (MQ=255)

                                                                                       ttCCGGACGAGCGGCTACGCAAAGTTGCTAAACCGGTAGAAGAAGAGAATGCAGAAATTCAGCGTATCGTCGATGATATGATCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCAt                                                <  1:1609453/140‑1 (MQ=255)

                                                                                        tCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATc                                               >  2:2572218/1‑140 (MQ=255)

                                                                                        tCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCAGGCGGCAACCCAGGTTGATATCCATc                                               >  2:3192201/1‑140 (MQ=255)

                                                                                         ccGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCa                                              <  1:5295622/140‑1 (MQ=255)

                                                                                         ccGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCa                                              <  2:4960026/140‑1 (MQ=255)

                                                                                          cGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCCGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCaa                                             >  2:2169973/1‑140 (MQ=255)

                                                                                          cGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCaa                                             >  1:825116/1‑140 (MQ=255)

                                                                                            gACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACg                                           >  1:4551181/1‑140 (MQ=255)

                                                                                             aCGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGt                                          <  1:3298242/140‑1 (MQ=255)

                                                                                               gAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTAt                                        <  2:6315267/140‑1 (MQ=255)

                                                                                               gAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTAt                                        <  2:4500067/140‑1 (MQ=255)

                                                                                                aGCGGCTTCGCAAAGTTGCTCAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATAAGTTCGAGACGATTTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCCACGTATc                                       >  2:5267049/1‑140 (MQ=255)

                                                                                                  cGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCAt                                     >  1:2914341/1‑140 (MQ=255)

                                                                                                             aGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATg                          <  1:4918892/140‑1 (MQ=255)

                                                                                                                       ccGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGaaaa                <  2:4776893/140‑1 (MQ=255)

                                                                                                                         ggTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAAcc              >  2:1167525/1‑140 (MQ=255)

                                                                                                                         ggTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAAcc              >  2:3741733/1‑140 (MQ=255)

                                                                                                                         ggTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAAcc              >  2:1550193/1‑140 (MQ=255)

                                                                                                                            agaagaagaGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTg           >  2:5066444/1‑140 (MQ=255)

                                                                                                                            agaagaAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACCATGTACGCAGAAGAAGGTAATGGCCTGGCGGAAACCCAGGTTGATATCCCACAACGTATCATTGTTATTGAAGTTTCGGAAAACCGTg           >  1:1956303/1‑140 (MQ=255)

                                                                                                                                gaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCANCCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCCGAAAANCGTGACGa       <  1:5710430/140‑1 (MQ=255)

                                                                                                                                gaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGa       <  2:4271348/140‑1 (MQ=255)

                                                                                                                                gaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGa       <  2:4625171/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  1:716345/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  2:1901688/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  1:2393751/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  1:1686950/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  2:3643256/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGAGGTACGCAGAAGAAGGTATGGGCCTGGCGGCAACCCAGGTTGATATCCATCACCGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  2:1627070/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATAGGTTCGAGACGATGTACGCAGAAGAAGGGATTCGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  2:5965562/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATAGTCGATGATATGTTCGAGAAGATGTAAGCAGAAGAAGGTATTGGACTGTCTGCAACACAGGTTGATATCAATAAACGTATCATTGTTATTTATGTTTCTGAAAACCGTGACGatagga  <  2:1296429/140‑6 (MQ=255)

GGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATACAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGC  >  NZ_CP025268/3434314‑3434586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`