BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,504,638	A→G	262 (TAA→TAG)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,504,638	0	A	G	99.1%	1097.5 / NA	318	262 (TAA→TAG)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (125/190); minor base T (2/1); total (127/191)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

ATCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCCTC  >  NZ_CP025268/3504501‑3504773

 tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                      <  2:2595315/140‑1 (MQ=255)

 tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                      <  1:5161187/140‑1 (MQ=255)

 tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                      <  1:1928208/140‑1 (MQ=255)

 tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                      <  1:6428301/140‑1 (MQ=255)

    acacCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATaa                                                                                                                                   >  2:3662130/1‑140 (MQ=255)

       ccATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGt                                                                                                                                <  1:32375/140‑1 (MQ=255)

       ccATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGt                                                                                                                                <  1:5274116/140‑1 (MQ=255)

        cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACgat                                                                                                                               >  1:5009044/1‑138 (MQ=255)

        cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGtt                                                                                                                               <  1:4404597/140‑1 (MQ=255)

        cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGtt                                                                                                                               >  1:2227717/1‑140 (MQ=255)

        cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGtt                                                                                                                               >  2:850643/1‑140 (MQ=255)

          tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGACAACCAGTCAGTACCACGGTGCCTGGTAGGTTTAACGGTgg                                                                                                                             >  2:6015907/1‑140 (MQ=255)

          tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTgg                                                                                                                             >  1:998328/1‑140 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGCTGACCTGAACGCAGGCGATAGCGCAGGGAACGGCGGGCGCGGCGACAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                            <  2:2673449/140‑1 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                            >  2:3312062/1‑140 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                            >  2:3312729/1‑140 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                            <  1:4530585/140‑1 (MQ=255)

            ggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCg                                                                                                                           <  1:6303501/140‑1 (MQ=255)

             gTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGt                                                                                                                          >  1:2433257/1‑140 (MQ=255)

             gTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGt                                                                                                                          >  1:3719403/1‑140 (MQ=255)

               gCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGa                                                                                                                        >  1:1736064/1‑140 (MQ=255)

               gCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGa                                                                                                                        >  1:6367510/1‑140 (MQ=255)

                ccGGAGATTCGTTCATTGCCGGAGTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAg                                                                                                                       <  2:5099633/140‑1 (MQ=255)

                  ggAGATTCGTTCATTGCCGGATTCCTTTGCGTCTGGTCTGAGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGAGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCa                                                                                                                     >  2:6400849/1‑140 (MQ=255)

                    agaTTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATc                                                                                                                   >  2:807307/1‑140 (MQ=255)

                          gTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACg                                                                                                             <  2:2003154/140‑1 (MQ=255)

                          gTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACg                                                                                                             <  2:4741380/140‑1 (MQ=255)

                           ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGc                                                                                                            >  1:1646377/1‑140 (MQ=255)

                           ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGc                                                                                                            >  2:3602548/1‑140 (MQ=255)

                           ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGc                                                                                                            <  2:3719403/140‑1 (MQ=255)

                             cagtGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                          <  2:2826743/137‑1 (MQ=255)

                               ttGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGGAGGTATAACGTTGGCGTGAGCATCTTCACGCCAAc                                                                                                        <  2:4681570/140‑1 (MQ=255)

                                     gATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTg                                                                                                  >  1:619904/1‑140 (MQ=255)

                                     gATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTg                                                                                                  <  1:308771/140‑1 (MQ=255)

                                         ccTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTAc                                                                                              <  1:4645611/140‑1 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                             <  1:288003/130‑1 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                             <  1:2524647/130‑1 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                             >  2:288003/1‑130 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                   <  2:136022/140‑1 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                   >  2:1991711/1‑140 (MQ=255)

                                                      tCTGGGGGGATGACATTACCGCAGGCGATAGCGCTGGTAACGGCGTGCGCGGCGAAAACCATTCAGTACCAGGGTGCCTGGTTGGTATAACGTTGGCGTGAGCATCTTCACGCCAAGGTGCTGTTACTTGCCGTAAAACg                                                                                 <  1:4866710/140‑1 (MQ=255)

                                                      tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                 <  2:6441762/140‑1 (MQ=255)

                                                      tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCGTGTAGGTATAACGTGGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                 <  2:5019833/140‑1 (MQ=255)

                                                      gcTGCGGGGATGACATTACCGCTGGCGATAGCGCAGGTAACGGCGTGCGCGGCTAAAACATTTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCAAAGGTGCTGTTACTTGCCGGAAAACg                                                                                 <  1:1010386/139‑1 (MQ=255)

                                                       cTGCGGGGAGGCCATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCCTTCAGTACCTCGTGGCCTGGTAGGTATACCGTTGGCGTGAGCATCTTCACGCCACCGTGATGTTACTGGCCGGAAAACGa                                                                                <  2:932777/140‑1 (MQ=255)

                                                          cGGGGATGACATTACCGCAGGCGATCGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGAccc                                                                             >  2:5498589/1‑140 (MQ=255)

                                                          cGGGGATGACATTACCGCAGGCGATAGCGCAGGTAACGGCGTGCGCGGCTAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGGTTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGAccc                                                                             <  1:2603847/140‑1 (MQ=255)

                                                           ggggATGACATTACCGCTGGCGTTAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCt                                                                            <  2:4665516/140‑1 (MQ=255)

                                                            gggATGACATTACCGCAGGCGATAGCGCACGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCta                                                                           >  2:5513971/1‑140 (MQ=255)

                                                               aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCAACTTCACGCCAACGTGCTGTTACTAGCCGGAAAACGACCCTATaa                                                                        >  2:5234641/1‑140 (MQ=255)

                                                                             ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcat                                                          >  2:5919990/1‑140 (MQ=255)

                                                                             ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcat                                                          >  1:2978264/1‑140 (MQ=255)

                                                                             ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcat                                                          >  2:4901556/1‑140 (MQ=255)

                                                                              gCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                         >  1:5355501/1‑140 (MQ=255)

                                                                                 aTAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCtt                                                      >  2:1644447/1‑140 (MQ=255)

                                                                                 aTAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCtt                                                      <  1:1383235/140‑1 (MQ=255)

                                                                                        aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                   >  1:5885632/1‑104 (MQ=255)

                                                                                        aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCa                                               >  1:5089242/1‑140 (MQ=255)

                                                                                        aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCa                                               >  1:1589483/1‑140 (MQ=255)

                                                                                        aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTACACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTACTCCATTCTTTATTTcg                                               >  2:571426/1‑139 (MQ=255)

                                                                                         gggAACGGCGTGCGCGGCGACAACCATTCAGTACCACGGTGCCTGGTAGGTATACCGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAg                                              <  2:4576408/140‑1 (MQ=255)

                                                                                          ggAACGGCGTGCGCGGCGAAAACAATTCAGTACCACGGTGCCTGTTAGGTATAACGTTGGCGTGAGCATCTGCACGCCAACNTGATTTTAATTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                             <  1:5696205/140‑1 (MQ=255)

                                                                                           gAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAggg                                            <  2:1762281/140‑1 (MQ=255)

                                                                                              cGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATCACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGG‑AAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGa                                        <  1:1563109/140‑1 (MQ=255)

                                                                                               ggCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGa                                        <  1:3312062/140‑1 (MQ=255)

                                                                                               ggCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGa                                        <  1:3312729/140‑1 (MQ=255)

                                                                                                 cGTGCTCGTCGAAAACCATTCTGTACGACGTTGGCTGTTATGTATAACTTTGGGGTGAGATTATTCACGCATAGGTGATGTTACTTGCAGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAtt                                      <  1:5509702/140‑1 (MQ=255)

                                                                                                  gTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTa                                     >  1:2328889/1‑140 (MQ=255)

                                                                                                     cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                  <  1:5598803/140‑1 (MQ=255)

                                                                                                      gcggcgAAAACCTTTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACTTGCTGTTACTTGCCGTAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTc                                 <  1:6059642/140‑1 (MQ=255)

                                                                                                        ggcgAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTACACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAg                               <  1:2756258/140‑1 (MQ=255)

                                                                                                         gcgAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGc                              <  1:616672/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                          <  1:5937485/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                          <  2:3071563/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                          <  2:2215270/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                          <  2:4123690/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                          <  1:688167/140‑1 (MQ=255)

                                                                                                                  aTTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCg                     >  2:4006293/1‑140 (MQ=255)

                                                                                                                  aTTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATATTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCg                     <  1:727475/140‑1 (MQ=255)

                                                                                                                    tCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCt                   >  1:2366922/1‑140 (MQ=255)

                                                                                                                       gTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGTAAAACGACCCTTTAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTAct                <  1:1186720/140‑1 (MQ=255)

                                                                                                                       gTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTAct                <  1:425888/140‑1 (MQ=255)

                                                                                                                          ccACGGTGCCTGGTAGGTATAACGTTGGCGAGAGCATCTTCACGCCAACGTGCTGTTCCTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCCGGGTCGATTATGTCAGCTACGGACCGCTACtctc             <  2:4387576/140‑1 (MQ=255)

                                                                                                                           cACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTACTTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACtctcg            >  2:3109563/1‑139 (MQ=255)

                                                                                                                            aCGGTGCCTGGTAGGTAGAACGTTGGCGTGATCATCTTCTCGCCAACGTGCTGTTACTTGCCGGATAACGACCCTAGCCTCCGAGTACTTCATTCTTTATGTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcat           <  2:5625189/140‑1 (MQ=255)

                                                                                                                             cGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcatc          >  2:5407398/1‑140 (MQ=255)

                                                                                                                             cGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCAAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcatc          <  1:5467121/140‑1 (MQ=255)

                                                                                                                               gTGCCTGGTAGGTATAACTTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa        <  1:8467/140‑1 (MQ=255)

                                                                                                                               gTGCCTGCTAGGGATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGGTACTTGCCGGCAAACGACCCTCTACTCCGAGTAATTCCTTCTTTATTTCAGGGTCGATTATGTCAGCAACGGACCGCTACTCTcaacaa        <  2:6338414/140‑1 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCaacaac       >  1:660730/1‑140 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc       >  2:2391469/1‑140 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc       <  1:4039387/140‑1 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc       >  1:2154758/1‑140 (MQ=255)

                                                                                                                                tGCCTGGTAGGGATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCGACTCTCATCAAc       >  2:1470149/1‑140 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                          <  2:2910228/119‑1 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc     <  1:807307/140‑1 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc     >  2:4266833/1‑140 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGCGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc     >  1:3792444/1‑140 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGACCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc     <  1:4994758/140‑1 (MQ=255)

                                                                                                                                     ggtaggtaTAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGACAACGACCCTATAATCCGAGTCATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCACctcctc  >  2:1916804/1‑140 (MQ=255)

ATCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCCTC  >  NZ_CP025268/3504501‑3504773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`